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The map label for this gene is gcvH [H]

Identifier: 220903438

GI number: 220903438

Start: 190703

End: 191095

Strand: Reverse

Name: gcvH [H]

Synonym: Ddes_0152

Alternate gene names: 220903438

Gene position: 191095-190703 (Counterclockwise)

Preceding gene: 220903439

Following gene: 220903437

Centisome position: 6.65

GC content: 59.03

Gene sequence:

>393_bases
ATGTCCAGCTACACACTGCCCGAAGACCGTTCCTATACTGATGAGCATATCTGGCTGCGCGAAGACGCCCAGGGCCTTAC
CGTGGGCATTTCAGATTTTGCCCAGGCCCAGCTTGAAGAAGTGGTCTACGTGGACATGCCCGCCGTGGGCAGCGCCGTTG
AGGCGGGAAAAGAATTCGGCTCCGTGGAATCAATGAAATCCGTCAGCGCGCTGTTCTCGCCTGTTTCCGGCGTGGTAGAA
GCGGTCAACACCGACCTTGAAAACAAACCGGACCTCGTCAACAGCGACTGTTACGGCCAGGGCTGGATCCTGCGCCTGCG
CAGGGAAGGCGACAATGCCGTGCCGCTGCTTTCCGCCGAAGAATACCGGACCCACCTGAAGTCACAGGCCTGA

Upstream 100 bases:

>100_bases
CGCATGCCAGGTTGACACACCCCGCCCTGTCAGGACCGCCGCCGCGCCCCACACTACGGGAGGCGCGGCGGCTTTTCACA
CCCGATAATGAGGAGAACCC

Downstream 100 bases:

>100_bases
GGCTGTTTACAAACAGGTCATTTTGCGCCACGAACAAGGGAATACGGCACCTGACGACAGGCACACGTCCTTATGGTATG
CCACTGAAAACAACGCCCCA

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 130; Mature: 129

Protein sequence:

>130_residues
MSSYTLPEDRSYTDEHIWLREDAQGLTVGISDFAQAQLEEVVYVDMPAVGSAVEAGKEFGSVESMKSVSALFSPVSGVVE
AVNTDLENKPDLVNSDCYGQGWILRLRREGDNAVPLLSAEEYRTHLKSQA

Sequences:

>Translated_130_residues
MSSYTLPEDRSYTDEHIWLREDAQGLTVGISDFAQAQLEEVVYVDMPAVGSAVEAGKEFGSVESMKSVSALFSPVSGVVE
AVNTDLENKPDLVNSDCYGQGWILRLRREGDNAVPLLSAEEYRTHLKSQA
>Mature_129_residues
SSYTLPEDRSYTDEHIWLREDAQGLTVGISDFAQAQLEEVVYVDMPAVGSAVEAGKEFGSVESMKSVSALFSPVSGVVEA
VNTDLENKPDLVNSDCYGQGWILRLRREGDNAVPLLSAEEYRTHLKSQA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=120, Percent_Identity=40, Blast_Score=97, Evalue=3e-21,
Organism=Homo sapiens, GI89057342, Length=120, Percent_Identity=39.1666666666667, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1789271, Length=127, Percent_Identity=40.1574803149606, Blast_Score=99, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17551294, Length=120, Percent_Identity=37.5, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17507493, Length=122, Percent_Identity=37.7049180327869, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319272, Length=126, Percent_Identity=37.3015873015873, Blast_Score=89, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17865652, Length=125, Percent_Identity=35.2, Blast_Score=87, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14271; Mature: 14140

Theoretical pI: Translated: 4.12; Mature: 4.12

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSYTLPEDRSYTDEHIWLREDAQGLTVGISDFAQAQLEEVVYVDMPAVGSAVEAGKEFG
CCCCCCCCCCCCCCCEEEEECCCCCEEECHHHHHHHHHHHEEEECCCCCCHHHHHHHHHC
SVESMKSVSALFSPVSGVVEAVNTDLENKPDLVNSDCYGQGWILRLRREGDNAVPLLSAE
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHH
EYRTHLKSQA
HHHHHHHCCC
>Mature Secondary Structure 
SSYTLPEDRSYTDEHIWLREDAQGLTVGISDFAQAQLEEVVYVDMPAVGSAVEAGKEFG
CCCCCCCCCCCCCCEEEEECCCCCEEECHHHHHHHHHHHEEEECCCCCCHHHHHHHHHC
SVESMKSVSALFSPVSGVVEAVNTDLENKPDLVNSDCYGQGWILRLRREGDNAVPLLSAE
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHH
EYRTHLKSQA
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA