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The map label for this gene is purB [H]

Identifier: 220903381

GI number: 220903381

Start: 121680

End: 122990

Strand: Direct

Name: purB [H]

Synonym: Ddes_0095

Alternate gene names: 220903381

Gene position: 121680-122990 (Clockwise)

Preceding gene: 220903380

Following gene: 220903382

Centisome position: 4.23

GC content: 60.56

Gene sequence:

>1311_bases
ATGATCGAACGCTATACCCGCCCGGAAATGGACCGTATATGGACTTTGAAAAACCGCTATCAGGCATGGCTTGACGTTGA
GCTGGCCGTTTGCCGGGCGTGGAGCGAAATGGGCCGGATCCCGGCCGAGGCCGTTGCCGTCATCTGCGACAAGGCTGCCA
TTGATGTGGACCGCATTCTCGAAATCGAGGAAGTAACCCGCCATGACGTCATCGCCTTTCTGACCTCGCTGGAAGAAAAG
GTAGGGCCGGATGCGCGCTACATCCACCTGGGCTGCACCTCGTCAGATATTGTGGACACGGCCAACGCCCTGCTGCTGGT
GCAGGCGGGCGAACTGATTCTCAAAGACATACGCGGGCTTCTGGGCAGCATTGAATCGCTGGCCCGGCGCCACAAGGGTG
TGCTGTGCATGGGCCGCACCCACGGCATCCACGCGGAACCCACCAGCTTCGGCCTCAAGATGGCCGGTTTTTACGCCGAA
TTTTCGCGTCATCTGGCGCGGGTGGAAGCCGGGCTTGAGAGCGTGCGCGTGGGCAAGATATCGGGCGCGGTGGGCACATA
CGCCTTTTTGTCGCCCGAACTGGAAGCGCGCGCCCTGGGCCATCTGGGCCTTGGGGTTGATCCGCACTCCACCCAGATAA
TCCAGCGCGACCGCTACGCCCACTTCTTTACCTCTCTGGCGATTCTGGGCGGCGGCATAGAGCGCCTTTGTGTGGAACTG
CGCCATCTGCAGCGCACCGAGGTGCTGGAAGTGGAAGAGGGCTTTGCCAGAGGGCAGAAAGGCTCCTCGGCCATGCCGCA
CAAAAAGAACCCCATTTCGGCAGAAAACATGACCGGGCTTTCACGCCTGCTGCGTACCAACGCCCTTGCCGCTCTGGAAA
ACCAGGCTCTCTGGCATGAGCGCGACATCAGCCATTCGTCGGTAGAGCGCGTTATCATGCCCGATTCCACCATACTGGCC
GACTACATTCTTGCGCGCCTTACCCGCCTTCTGGACGGCCTTGTGGTCAAGCCCGACCGCATGCGCGAAAATATGGACCG
TTCTTTTGGCCTGTATTTTTCGCAGCGTGTGCTCACGGCGCTTGTGTCCGGCGGGCTGCCGCGCCAGCAGGCCTACGAGG
CCGTGCAGCGTCTTGCCATGCAGAGCTGGGAAAGCCGCGTGCCTTTCCCCGATCTTGTGCGCGCCGATGCGGATATGAGC
AGCCGTCTGGGCGAAGCCGCCCTGAAAGACCTTTTTGATCCTTCCTATTACCTGCAACACGAAGATGAAATTTTCGCCCG
CGTGTTTGCTGAAAGCCCTGCCCGGGCCTAG

Upstream 100 bases:

>100_bases
GCCGCGCCGGCCGCACCCGCACCTGCCGCCGCCCCGGCGGCCGCTCCCGCAACCCCGGCCCCTGCTGCCGGCCCCGCGAA
AACGGATTCATGAGGCTGTC

Downstream 100 bases:

>100_bases
CCGAGGTTGTTCATGAGCGTTTGCACCCAAGAGCATATGATGGAACTTAAACGCCGTCTCGCGCGCCTGCTGGTGGAAAA
ATCCTATCGTGAAGGGGATT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MIERYTRPEMDRIWTLKNRYQAWLDVELAVCRAWSEMGRIPAEAVAVICDKAAIDVDRILEIEEVTRHDVIAFLTSLEEK
VGPDARYIHLGCTSSDIVDTANALLLVQAGELILKDIRGLLGSIESLARRHKGVLCMGRTHGIHAEPTSFGLKMAGFYAE
FSRHLARVEAGLESVRVGKISGAVGTYAFLSPELEARALGHLGLGVDPHSTQIIQRDRYAHFFTSLAILGGGIERLCVEL
RHLQRTEVLEVEEGFARGQKGSSAMPHKKNPISAENMTGLSRLLRTNALAALENQALWHERDISHSSVERVIMPDSTILA
DYILARLTRLLDGLVVKPDRMRENMDRSFGLYFSQRVLTALVSGGLPRQQAYEAVQRLAMQSWESRVPFPDLVRADADMS
SRLGEAALKDLFDPSYYLQHEDEIFARVFAESPARA

Sequences:

>Translated_436_residues
MIERYTRPEMDRIWTLKNRYQAWLDVELAVCRAWSEMGRIPAEAVAVICDKAAIDVDRILEIEEVTRHDVIAFLTSLEEK
VGPDARYIHLGCTSSDIVDTANALLLVQAGELILKDIRGLLGSIESLARRHKGVLCMGRTHGIHAEPTSFGLKMAGFYAE
FSRHLARVEAGLESVRVGKISGAVGTYAFLSPELEARALGHLGLGVDPHSTQIIQRDRYAHFFTSLAILGGGIERLCVEL
RHLQRTEVLEVEEGFARGQKGSSAMPHKKNPISAENMTGLSRLLRTNALAALENQALWHERDISHSSVERVIMPDSTILA
DYILARLTRLLDGLVVKPDRMRENMDRSFGLYFSQRVLTALVSGGLPRQQAYEAVQRLAMQSWESRVPFPDLVRADADMS
SRLGEAALKDLFDPSYYLQHEDEIFARVFAESPARA
>Mature_436_residues
MIERYTRPEMDRIWTLKNRYQAWLDVELAVCRAWSEMGRIPAEAVAVICDKAAIDVDRILEIEEVTRHDVIAFLTSLEEK
VGPDARYIHLGCTSSDIVDTANALLLVQAGELILKDIRGLLGSIESLARRHKGVLCMGRTHGIHAEPTSFGLKMAGFYAE
FSRHLARVEAGLESVRVGKISGAVGTYAFLSPELEARALGHLGLGVDPHSTQIIQRDRYAHFFTSLAILGGGIERLCVEL
RHLQRTEVLEVEEGFARGQKGSSAMPHKKNPISAENMTGLSRLLRTNALAALENQALWHERDISHSSVERVIMPDSTILA
DYILARLTRLLDGLVVKPDRMRENMDRSFGLYFSQRVLTALVSGGLPRQQAYEAVQRLAMQSWESRVPFPDLVRADADMS
SRLGEAALKDLFDPSYYLQHEDEIFARVFAESPARA

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=395, Percent_Identity=25.8227848101266, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI183227688, Length=414, Percent_Identity=25.3623188405797, Blast_Score=125, Evalue=8e-29,
Organism=Escherichia coli, GI1787376, Length=400, Percent_Identity=30, Blast_Score=111, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17508577, Length=382, Percent_Identity=26.4397905759162, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI32564234, Length=327, Percent_Identity=27.5229357798165, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323391, Length=402, Percent_Identity=25.8706467661692, Blast_Score=136, Evalue=8e-33,
Organism=Drosophila melanogaster, GI24647570, Length=396, Percent_Identity=27.020202020202, Blast_Score=116, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48704; Mature: 48704

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYTRPEMDRIWTLKNRYQAWLDVELAVCRAWSEMGRIPAEAVAVICDKAAIDVDRIL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHH
EIEEVTRHDVIAFLTSLEEKVGPDARYIHLGCTSSDIVDTANALLLVQAGELILKDIRGL
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCEEEEHHHHHHHHHHHHH
LGSIESLARRHKGVLCMGRTHGIHAEPTSFGLKMAGFYAEFSRHLARVEAGLESVRVGKI
HHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SGAVGTYAFLSPELEARALGHLGLGVDPHSTQIIQRDRYAHFFTSLAILGGGIERLCVEL
CCCHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RHLQRTEVLEVEEGFARGQKGSSAMPHKKNPISAENMTGLSRLLRTNALAALENQALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSVERVIMPDSTILADYILARLTRLLDGLVVKPDRMRENMDRSFGLYFSQRVLTA
HCCCHHHHCEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LVSGGLPRQQAYEAVQRLAMQSWESRVPFPDLVRADADMSSRLGEAALKDLFDPSYYLQH
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCHHHHCC
EDEIFARVFAESPARA
HHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIERYTRPEMDRIWTLKNRYQAWLDVELAVCRAWSEMGRIPAEAVAVICDKAAIDVDRIL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHH
EIEEVTRHDVIAFLTSLEEKVGPDARYIHLGCTSSDIVDTANALLLVQAGELILKDIRGL
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCEEEEHHHHHHHHHHHHH
LGSIESLARRHKGVLCMGRTHGIHAEPTSFGLKMAGFYAEFSRHLARVEAGLESVRVGKI
HHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SGAVGTYAFLSPELEARALGHLGLGVDPHSTQIIQRDRYAHFFTSLAILGGGIERLCVEL
CCCHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RHLQRTEVLEVEEGFARGQKGSSAMPHKKNPISAENMTGLSRLLRTNALAALENQALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDISHSSVERVIMPDSTILADYILARLTRLLDGLVVKPDRMRENMDRSFGLYFSQRVLTA
HCCCHHHHCEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LVSGGLPRQQAYEAVQRLAMQSWESRVPFPDLVRADADMSSRLGEAALKDLFDPSYYLQH
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCHHHHCC
EDEIFARVFAESPARA
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]