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The map label for this gene is pyrE [H]

Identifier: 220903382

GI number: 220903382

Start: 123003

End: 123584

Strand: Direct

Name: pyrE [H]

Synonym: Ddes_0096

Alternate gene names: 220903382

Gene position: 123003-123584 (Clockwise)

Preceding gene: 220903381

Following gene: 220903383

Centisome position: 4.28

GC content: 58.59

Gene sequence:

>582_bases
ATGAGCGTTTGCACCCAAGAGCATATGATGGAACTTAAACGCCGTCTCGCGCGCCTGCTGGTGGAAAAATCCTATCGTGA
AGGGGATTTTATGCTGGCTTCAGGCCGCAGAAGCGACTATTATTTTGACTGTCGTGTAACGGCCTTGCACGCCGAAGGCT
CCTGGCTTATCGGCACGCTGTTCAACCACATGCTCCGCGATCTGGATATCCGGGGTGTGGGCGGCATGACCATGGGGGCA
GATCCGCTGGTTTCCGCCACTACGGTTGTTTCGCATGAGCAGGGCAGGCCCCTGCACGGCCTGCTGGTGCGCAAAGAGGC
CAAAGGTCACGGCACCGGGCAGTTTGTGGAAGGACTTGGCAATTTTAACGCAGGCGATCGCGTTGCCATGCTGGAAGATG
TGGTCACCACGGGCGGTTCGCTGCTCAAGGCCTGTCACAGGATCAGCGACGCAGAGCTGAACATTGTTGCTGTGTGCGCC
ATTCTGGACAGGGAGGAAGGTGGCCGTGAAAAGCTCCGTGAGGCGGGATATGAACTGCTTGCCCTCTTCACCCGCGCGGA
ACTGGTGGCCCTTGCGCGCTGA

Upstream 100 bases:

>100_bases
GAAAGACCTTTTTGATCCTTCCTATTACCTGCAACACGAAGATGAAATTTTCGCCCGCGTGTTTGCTGAAAGCCCTGCCC
GGGCCTAGCCGAGGTTGTTC

Downstream 100 bases:

>100_bases
GTGCACCCAGGCGGAACCTGACGCAGCAGCCCTCGGGGCTGTCGTGCCTGAGGGGACTTCGCGGGCGTCTTGTTTGCCGT
ACGCGGACGGGCGCACGCCC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MSVCTQEHMMELKRRLARLLVEKSYREGDFMLASGRRSDYYFDCRVTALHAEGSWLIGTLFNHMLRDLDIRGVGGMTMGA
DPLVSATTVVSHEQGRPLHGLLVRKEAKGHGTGQFVEGLGNFNAGDRVAMLEDVVTTGGSLLKACHRISDAELNIVAVCA
ILDREEGGREKLREAGYELLALFTRAELVALAR

Sequences:

>Translated_193_residues
MSVCTQEHMMELKRRLARLLVEKSYREGDFMLASGRRSDYYFDCRVTALHAEGSWLIGTLFNHMLRDLDIRGVGGMTMGA
DPLVSATTVVSHEQGRPLHGLLVRKEAKGHGTGQFVEGLGNFNAGDRVAMLEDVVTTGGSLLKACHRISDAELNIVAVCA
ILDREEGGREKLREAGYELLALFTRAELVALAR
>Mature_192_residues
SVCTQEHMMELKRRLARLLVEKSYREGDFMLASGRRSDYYFDCRVTALHAEGSWLIGTLFNHMLRDLDIRGVGGMTMGAD
PLVSATTVVSHEQGRPLHGLLVRKEAKGHGTGQFVEGLGNFNAGDRVAMLEDVVTTGGSLLKACHRISDAELNIVAVCAI
LDREEGGREKLREAGYELLALFTRAELVALAR

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=171, Percent_Identity=31.5789473684211, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17554964, Length=173, Percent_Identity=36.4161849710983, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17508631, Length=174, Percent_Identity=29.8850574712644, Blast_Score=70, Evalue=5e-13,
Organism=Drosophila melanogaster, GI17933654, Length=174, Percent_Identity=35.632183908046, Blast_Score=87, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 21262; Mature: 21131

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVCTQEHMMELKRRLARLLVEKSYREGDFMLASGRRSDYYFDCRVTALHAEGSWLIGTL
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEEEEEEECCCCCHHHHH
FNHMLRDLDIRGVGGMTMGADPLVSATTVVSHEQGRPLHGLLVRKEAKGHGTGQFVEGLG
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHEEEECCCCCCCHHHHHHHC
NFNAGDRVAMLEDVVTTGGSLLKACHRISDAELNIVAVCAILDREEGGREKLREAGYELL
CCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEHHCCCCCCHHHHHHHHHHHH
ALFTRAELVALAR
HHHHHHHHHHHCC
>Mature Secondary Structure 
SVCTQEHMMELKRRLARLLVEKSYREGDFMLASGRRSDYYFDCRVTALHAEGSWLIGTL
CCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEEEEEEECCCCCHHHHH
FNHMLRDLDIRGVGGMTMGADPLVSATTVVSHEQGRPLHGLLVRKEAKGHGTGQFVEGLG
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHEEEECCCCCCCHHHHHHHC
NFNAGDRVAMLEDVVTTGGSLLKACHRISDAELNIVAVCAILDREEGGREKLREAGYELL
CCCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEEEEHHCCCCCCHHHHHHHHHHHH
ALFTRAELVALAR
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]