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The map label for this gene is purF [H]

Identifier: 220903294

GI number: 220903294

Start: 11489

End: 12892

Strand: Direct

Name: purF [H]

Synonym: Ddes_0008

Alternate gene names: 220903294

Gene position: 11489-12892 (Clockwise)

Preceding gene: 220903293

Following gene: 220903295

Centisome position: 0.4

GC content: 56.13

Gene sequence:

>1404_bases
ATGATTAAGCACGAATGCGGTGTTTTCGGCATTTATGACCATGACGATGCTGCCCGTCTGGCCTATTTCGGCCTTTATGC
CCAGCAGCACCGGGGACAGGAAAGCGCGGGCATAGTCACTTTTGACGGCAAGGCCGTCAATGAACACAGGGGTATGGGCC
TTGTACCCGACGTGTTTTCCGAAGCTGACCTCCAGCGTCTTGTGGGGCGTCACGCTGTGGGGCATGTGCGCTACTCCACC
ACAGGCAGTTCGTCCAGCGGCAACGCCCAGCCTTTTCTTGCCAATTACAAGGGCAGGGCCATTGCGCTGGCCCATAACGG
CAACCTGGTCAACGCCGCCCAACTGCGCGAAGACCTGGAAAACGAAGGGGCCATTTTTTCTACCAGCAATGACACTGAAG
TGTTCATGCACCTTCTGGTACGCGCCCTGCGCCACAATGATCTGCCTGGGGCCGTCAAGGAAGCCTGTTACAGAGTGCAG
GGGGCCTATTGCCTTCTGGTCATGGCTGACGGCGTGATGGTAGCCGTGCGCGACCCCCACGGTTTTCATCCGCTTGCCTT
CGGCCGCATGAACGGCAGCCCTGTCTTTGCCTCTGAAACCTGTGCCTTTGACCTGCTTGAAGCCGAATTCGTGCGTACGG
TTGAACCGGGCGAAGTGGTTGTGGTGGAAGGCAACAGCGTGCGCAGCGAGCGCCTCATAGGTCCCATGCCCGAAAAGCCC
GCCCAGTGCATTTTTGAGCTGATCTATTTTGCCCGGCCGGACTCGTATGTTTTTGACGAACAGGTGTATCAATGCCGTAA
AAAAATGGGCTGGAACCTGGCGATGGAATCCGCGCCGGATGTGGATTTTGTCATGCCTTTTCCTGACTCGGGTATTTACC
CGGCCCTGGGGTTCGCGCAGCGTTCCGGCCTGCACTACGAGCATGCCATGATACGCAACCACTATGTTGGGCGCACGTTT
ATCCAGCCTTCGCAGAGCATGCGCAGCTTCGGCGTACGGGTAAAGATCAATCCCGTGCGTGAAATGATAGAAGGAAAGCG
CATCTGCATTATCGACGACAGTATTGTACGCGGCACAACCATGATGACCCGTGTAAAAAAACTGCGCGAGCTGGGGGCAA
AAGAGGTGCACATACGTATTTCTTCTCCGCCGGTCATGTCGCCCTGTCACTACGGCATCGACTTCTCATCGCGGGGAGAG
CTTATTGCCGCGCAACACAGCCTGGAAGAGATACAGCGCAAACTGGATGTGGATTCCCTGCATTATCTGAGTATTGAAGG
GCTCATGGGGTCTGTGAGTAAACCGCAGCACTACTGTATGGCCTGCTTTACCGGAGAGTACCCCGTGCCTTGCGAGGAAT
GCGGCGACAAGTTTAAGCTTGAAGGCTCCTGCGGTTCACGCTAA

Upstream 100 bases:

>100_bases
CCCTGAAACAGTTTGAACTGGCGCGCGACGCATACCCCAATCCGTCGGTCATCGACAATCGCATTGCGCATTTGCGCAAA
AAACTGAAGAAGTAAGGCTT

Downstream 100 bases:

>100_bases
TTTCTGGCCCCGGGCCGAAATTCAGGGCCGGGAGCGCCGGAACATTTTAGTTTTGGGATGTATATGCTCAAAGAATTACG
GCGCTCGCTGTGGCCTTGAC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MIKHECGVFGIYDHDDAARLAYFGLYAQQHRGQESAGIVTFDGKAVNEHRGMGLVPDVFSEADLQRLVGRHAVGHVRYST
TGSSSSGNAQPFLANYKGRAIALAHNGNLVNAAQLREDLENEGAIFSTSNDTEVFMHLLVRALRHNDLPGAVKEACYRVQ
GAYCLLVMADGVMVAVRDPHGFHPLAFGRMNGSPVFASETCAFDLLEAEFVRTVEPGEVVVVEGNSVRSERLIGPMPEKP
AQCIFELIYFARPDSYVFDEQVYQCRKKMGWNLAMESAPDVDFVMPFPDSGIYPALGFAQRSGLHYEHAMIRNHYVGRTF
IQPSQSMRSFGVRVKINPVREMIEGKRICIIDDSIVRGTTMMTRVKKLRELGAKEVHIRISSPPVMSPCHYGIDFSSRGE
LIAAQHSLEEIQRKLDVDSLHYLSIEGLMGSVSKPQHYCMACFTGEYPVPCEECGDKFKLEGSCGSR

Sequences:

>Translated_467_residues
MIKHECGVFGIYDHDDAARLAYFGLYAQQHRGQESAGIVTFDGKAVNEHRGMGLVPDVFSEADLQRLVGRHAVGHVRYST
TGSSSSGNAQPFLANYKGRAIALAHNGNLVNAAQLREDLENEGAIFSTSNDTEVFMHLLVRALRHNDLPGAVKEACYRVQ
GAYCLLVMADGVMVAVRDPHGFHPLAFGRMNGSPVFASETCAFDLLEAEFVRTVEPGEVVVVEGNSVRSERLIGPMPEKP
AQCIFELIYFARPDSYVFDEQVYQCRKKMGWNLAMESAPDVDFVMPFPDSGIYPALGFAQRSGLHYEHAMIRNHYVGRTF
IQPSQSMRSFGVRVKINPVREMIEGKRICIIDDSIVRGTTMMTRVKKLRELGAKEVHIRISSPPVMSPCHYGIDFSSRGE
LIAAQHSLEEIQRKLDVDSLHYLSIEGLMGSVSKPQHYCMACFTGEYPVPCEECGDKFKLEGSCGSR
>Mature_467_residues
MIKHECGVFGIYDHDDAARLAYFGLYAQQHRGQESAGIVTFDGKAVNEHRGMGLVPDVFSEADLQRLVGRHAVGHVRYST
TGSSSSGNAQPFLANYKGRAIALAHNGNLVNAAQLREDLENEGAIFSTSNDTEVFMHLLVRALRHNDLPGAVKEACYRVQ
GAYCLLVMADGVMVAVRDPHGFHPLAFGRMNGSPVFASETCAFDLLEAEFVRTVEPGEVVVVEGNSVRSERLIGPMPEKP
AQCIFELIYFARPDSYVFDEQVYQCRKKMGWNLAMESAPDVDFVMPFPDSGIYPALGFAQRSGLHYEHAMIRNHYVGRTF
IQPSQSMRSFGVRVKINPVREMIEGKRICIIDDSIVRGTTMMTRVKKLRELGAKEVHIRISSPPVMSPCHYGIDFSSRGE
LIAAQHSLEEIQRKLDVDSLHYLSIEGLMGSVSKPQHYCMACFTGEYPVPCEECGDKFKLEGSCGSR

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=509, Percent_Identity=39.8821218074656, Blast_Score=337, Evalue=1e-92,
Organism=Escherichia coli, GI1788651, Length=469, Percent_Identity=36.8869936034115, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1790167, Length=230, Percent_Identity=29.1304347826087, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17554892, Length=484, Percent_Identity=40.2892561983471, Blast_Score=322, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6323958, Length=478, Percent_Identity=33.2635983263598, Blast_Score=233, Evalue=6e-62,
Organism=Drosophila melanogaster, GI28573187, Length=488, Percent_Identity=39.5491803278689, Blast_Score=330, Evalue=9e-91,
Organism=Drosophila melanogaster, GI24659598, Length=482, Percent_Identity=40.0414937759336, Blast_Score=329, Evalue=3e-90,
Organism=Drosophila melanogaster, GI24659604, Length=458, Percent_Identity=40.174672489083, Blast_Score=321, Evalue=6e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51868; Mature: 51868

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKHECGVFGIYDHDDAARLAYFGLYAQQHRGQESAGIVTFDGKAVNEHRGMGLVPDVFS
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCHHHHH
EADLQRLVGRHAVGHVRYSTTGSSSSGNAQPFLANYKGRAIALAHNGNLVNAAQLREDLE
HHHHHHHHHHHHHCEEEEECCCCCCCCCCCCEEECCCCCEEEEEECCCEEEHHHHHHHHH
NEGAIFSTSNDTEVFMHLLVRALRHNDLPGAVKEACYRVQGAYCLLVMADGVMVAVRDPH
CCCCEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCEEEEEECCC
GFHPLAFGRMNGSPVFASETCAFDLLEAEFVRTVEPGEVVVVEGNSVRSERLIGPMPEKP
CCCCEEEECCCCCCEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCH
AQCIFELIYFARPDSYVFDEQVYQCRKKMGWNLAMESAPDVDFVMPFPDSGIYPALGFAQ
HHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCEEEECCCCCCCCCCCCHHH
RSGLHYEHAMIRNHYVGRTFIQPSQSMRSFGVRVKINPVREMIEGKRICIIDDSIVRGTT
HCCCCHHHHHHHHCCCCCEEECCCHHHHHCCCEEEEHHHHHHHCCCEEEEECCCHHCCHH
MMTRVKKLRELGAKEVHIRISSPPVMSPCHYGIDFSSRGELIAAQHSLEEIQRKLDVDSL
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCE
HYLSIEGLMGSVSKPQHYCMACFTGEYPVPCEECGDKFKLEGSCGSR
EEEEEHHHHCCCCCCCEEEEEEECCCCCCCHHHCCCEEEECCCCCCC
>Mature Secondary Structure
MIKHECGVFGIYDHDDAARLAYFGLYAQQHRGQESAGIVTFDGKAVNEHRGMGLVPDVFS
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCHHHHH
EADLQRLVGRHAVGHVRYSTTGSSSSGNAQPFLANYKGRAIALAHNGNLVNAAQLREDLE
HHHHHHHHHHHHHCEEEEECCCCCCCCCCCCEEECCCCCEEEEEECCCEEEHHHHHHHHH
NEGAIFSTSNDTEVFMHLLVRALRHNDLPGAVKEACYRVQGAYCLLVMADGVMVAVRDPH
CCCCEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCEEEEEECCC
GFHPLAFGRMNGSPVFASETCAFDLLEAEFVRTVEPGEVVVVEGNSVRSERLIGPMPEKP
CCCCEEEECCCCCCEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCH
AQCIFELIYFARPDSYVFDEQVYQCRKKMGWNLAMESAPDVDFVMPFPDSGIYPALGFAQ
HHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCEEEECCCCCCCCCCCCHHH
RSGLHYEHAMIRNHYVGRTFIQPSQSMRSFGVRVKINPVREMIEGKRICIIDDSIVRGTT
HCCCCHHHHHHHHCCCCCEEECCCHHHHHCCCEEEEHHHHHHHCCCEEEEECCCHHCCHH
MMTRVKKLRELGAKEVHIRISSPPVMSPCHYGIDFSSRGELIAAQHSLEEIQRKLDVDSL
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCCE
HYLSIEGLMGSVSKPQHYCMACFTGEYPVPCEECGDKFKLEGSCGSR
EEEEEHHHHCCCCCCCEEEEEEECCCCCCCHHHCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]