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The map label for this gene is hisS [H]

Identifier: 220903295

GI number: 220903295

Start: 13137

End: 14387

Strand: Direct

Name: hisS [H]

Synonym: Ddes_0009

Alternate gene names: 220903295

Gene position: 13137-14387 (Clockwise)

Preceding gene: 220903294

Following gene: 220903296

Centisome position: 0.46

GC content: 59.71

Gene sequence:

>1251_bases
ATGAGTATTGCACGTATCAAGGGCTTTGCGGACATGTTTCCGCCGGACAGCGACATCTTTACCCGCATGGAAAACACCGC
GCGGGATATTTTCGGCCGCTACGGCTTTGTGGAACTGCGTACGCCCATAGTGGAGTTTACCGAGCTTTTTCAGCGCTCCA
TCGGTGAAGAAACCGATGTGGTGCAAAAAGAAATGTATACCTTTCCCGACCGCAAGGACAGGTCGCTTACCCTGCGGCCG
GAGGCTACGGCGGGCGTTATGCGTGCCTACATTGAAAGCGGCCTTGCCAACAGGGAAGCGGTCAGCCGTCTGTTCACCAC
AGGTCCCATGTTCCGCTACGAGCGGCCGCAAAAGGGCCGCATGCGGCAGTTCCACCAGATCAACTGCGAGTGCCTGGGTT
CGCACAGCCCGTACGCCGATGCCGACCTTATCAGCATGCTGCTGCGTTTTCTTGATGCCCTGGGGCTTGAAGATCTGACC
CTCAAGGTCAACTCCCTGGGCTGTTCGCAGTGTCGTCCAAAGTTCAAGGACGCACTGCTGGCCTATCTTGAAACCGTGGA
CAGTGCCGCCCTCTGCGCGGACTGCGCCCGCAGGGTGCACACCAATCCCTTGCGTGTGCTGGACTGCAAACAGCCCGGCT
GCCGGGCCATCACCGATGCGGCCCCGAAACTGCTGGATTATAACTGTCCGGAGTGCAGGGCGCATTTTGATACGGTGCTC
GGCCTGCTTAACGGTGAAAAGCTGCCCTTTGAGCTTGACCACAGGCTCGTGCGCGGGCTTGACTACTATTGCCGCACCAC
CTTTGAGGTGGTAAGCGGCAGTATTGGCGCACAGGCGGCGGTGGCGGGCGGCGGCCGTTATGACGGGCTTATCAGGAGCC
TCGGCGGTCCTGATGCCCCTGGCGTGGGCTTTGCCGCCGGTATGGAGCGCCTGGCCCTTATGATGGGTGCCGGGGCGGCT
GTTCCGGCTGATTTTTACCTTGTGGGCATGGATGCGCAGAGCAGGGCGCAGGGTTACGCCCTGGCGCAAAACCTGCGCGC
CGCCGGCCTGCGGGGCGAGATGAATTTCAGTGAGGCGGGCTTTAAAAGTCTTATGCGGCAGGCCGGAAAATCCGGTGCGC
GGTATTGCCTCATCATGGGACCGGACGAAGCGGCCCAGAACACTGTGGTTGTAAAAAATCTGGAAAGCGGAGAACAGACC
ACTGTGCCGCAAGCCCAGGCACTACACATTTTGCAAACGGGAACCAACTGA

Upstream 100 bases:

>100_bases
GCAACGGCTGAAGCTGCGCACCTTGCAAAGACGTCTGCCTGTGCAGCGTCAGGGCAATGTTAAGGCGAAATTTTCTTATT
GCGCCACTATTGAGGACGAC

Downstream 100 bases:

>100_bases
CATGACTGAAGAAAGCCAGATGGCGGAGCAGACGGCCCAGGCGGTCGCGCAGGGCCAGGACGTACAGCAGGAACACCAGA
AGTATATCACCGGCCTTGGC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MSIARIKGFADMFPPDSDIFTRMENTARDIFGRYGFVELRTPIVEFTELFQRSIGEETDVVQKEMYTFPDRKDRSLTLRP
EATAGVMRAYIESGLANREAVSRLFTTGPMFRYERPQKGRMRQFHQINCECLGSHSPYADADLISMLLRFLDALGLEDLT
LKVNSLGCSQCRPKFKDALLAYLETVDSAALCADCARRVHTNPLRVLDCKQPGCRAITDAAPKLLDYNCPECRAHFDTVL
GLLNGEKLPFELDHRLVRGLDYYCRTTFEVVSGSIGAQAAVAGGGRYDGLIRSLGGPDAPGVGFAAGMERLALMMGAGAA
VPADFYLVGMDAQSRAQGYALAQNLRAAGLRGEMNFSEAGFKSLMRQAGKSGARYCLIMGPDEAAQNTVVVKNLESGEQT
TVPQAQALHILQTGTN

Sequences:

>Translated_416_residues
MSIARIKGFADMFPPDSDIFTRMENTARDIFGRYGFVELRTPIVEFTELFQRSIGEETDVVQKEMYTFPDRKDRSLTLRP
EATAGVMRAYIESGLANREAVSRLFTTGPMFRYERPQKGRMRQFHQINCECLGSHSPYADADLISMLLRFLDALGLEDLT
LKVNSLGCSQCRPKFKDALLAYLETVDSAALCADCARRVHTNPLRVLDCKQPGCRAITDAAPKLLDYNCPECRAHFDTVL
GLLNGEKLPFELDHRLVRGLDYYCRTTFEVVSGSIGAQAAVAGGGRYDGLIRSLGGPDAPGVGFAAGMERLALMMGAGAA
VPADFYLVGMDAQSRAQGYALAQNLRAAGLRGEMNFSEAGFKSLMRQAGKSGARYCLIMGPDEAAQNTVVVKNLESGEQT
TVPQAQALHILQTGTN
>Mature_415_residues
SIARIKGFADMFPPDSDIFTRMENTARDIFGRYGFVELRTPIVEFTELFQRSIGEETDVVQKEMYTFPDRKDRSLTLRPE
ATAGVMRAYIESGLANREAVSRLFTTGPMFRYERPQKGRMRQFHQINCECLGSHSPYADADLISMLLRFLDALGLEDLTL
KVNSLGCSQCRPKFKDALLAYLETVDSAALCADCARRVHTNPLRVLDCKQPGCRAITDAAPKLLDYNCPECRAHFDTVLG
LLNGEKLPFELDHRLVRGLDYYCRTTFEVVSGSIGAQAAVAGGGRYDGLIRSLGGPDAPGVGFAAGMERLALMMGAGAAV
PADFYLVGMDAQSRAQGYALAQNLRAAGLRGEMNFSEAGFKSLMRQAGKSGARYCLIMGPDEAAQNTVVVKNLESGEQTT
VPQAQALHILQTGTN

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788861, Length=421, Percent_Identity=47.9809976247031, Blast_Score=370, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 45605; Mature: 45474

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIARIKGFADMFPPDSDIFTRMENTARDIFGRYGFVELRTPIVEFTELFQRSIGEETDV
CCCHHHCCHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCHHHH
VQKEMYTFPDRKDRSLTLRPEATAGVMRAYIESGLANREAVSRLFTTGPMFRYERPQKGR
HHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHH
MRQFHQINCECLGSHSPYADADLISMLLRFLDALGLEDLTLKVNSLGCSQCRPKFKDALL
HHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHCCHHHHHHHH
AYLETVDSAALCADCARRVHTNPLRVLDCKQPGCRAITDAAPKLLDYNCPECRAHFDTVL
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHCHHHHCCCCHHHHHHHHHHH
GLLNGEKLPFELDHRLVRGLDYYCRTTFEVVSGSIGAQAAVAGGGRYDGLIRSLGGPDAP
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHCCCCCCC
GVGFAAGMERLALMMGAGAAVPADFYLVGMDAQSRAQGYALAQNLRAAGLRGEMNFSEAG
CCCHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
FKSLMRQAGKSGARYCLIMGPDEAAQNTVVVKNLESGEQTTVPQAQALHILQTGTN
HHHHHHHHCCCCCCEEEEECCCCHHCCEEEEEECCCCCCCCCCHHHHHHHEECCCC
>Mature Secondary Structure 
SIARIKGFADMFPPDSDIFTRMENTARDIFGRYGFVELRTPIVEFTELFQRSIGEETDV
CCHHHCCHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCHHHH
VQKEMYTFPDRKDRSLTLRPEATAGVMRAYIESGLANREAVSRLFTTGPMFRYERPQKGR
HHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHH
MRQFHQINCECLGSHSPYADADLISMLLRFLDALGLEDLTLKVNSLGCSQCRPKFKDALL
HHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHCCHHHHHHHH
AYLETVDSAALCADCARRVHTNPLRVLDCKQPGCRAITDAAPKLLDYNCPECRAHFDTVL
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHCHHHHCCCCHHHHHHHHHHH
GLLNGEKLPFELDHRLVRGLDYYCRTTFEVVSGSIGAQAAVAGGGRYDGLIRSLGGPDAP
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHCCCCCCC
GVGFAAGMERLALMMGAGAAVPADFYLVGMDAQSRAQGYALAQNLRAAGLRGEMNFSEAG
CCCHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
FKSLMRQAGKSGARYCLIMGPDEAAQNTVVVKNLESGEQTTVPQAQALHILQTGTN
HHHHHHHHCCCCCCEEEEECCCCHHCCEEEEEECCCCCCCCCCHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA