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The map label for this gene is phoB [C]

Identifier: 218906439

GI number: 218906439

Start: 5050872

End: 5051543

Strand: Reverse

Name: phoB [C]

Synonym: BCAH820_5353

Alternate gene names: 218906439

Gene position: 5051543-5050872 (Counterclockwise)

Preceding gene: 218906440

Following gene: 218906438

Centisome position: 95.26

GC content: 42.26

Gene sequence:

>672_bases
ATGAAAATACTTGTAGTGGACGATGAGTCGAGTATTCGCAATTTGATTCGGATGCAGCTTGAGATGGAAGGATATGAAGT
GCTCACGGCGGCCGATGGGAGAGAAGCTTTAGAGAGATGGAATGAAGGGCCGGATGTTTTGATTTTGGATGTTATGCTTC
CAGATACAGATGGGTATGAGTTGCTTCGTTTGTTCAGGGAGAAGGATAGAGATATTCCAGTACTCATGTTAACTGCGAAG
AGTCAGATGAATGATAAATTGCTTGGTTTGCAGCTTGGCGCTGATGATTACGTGACGAAGCCATTTAATTATGCGGAGCT
TATTCTTCGGGTCAAGAATATGACGCGGCGTGTGAAGAAGACAGAGGTAACGGTAAGTCATGAAGTAATCGAGGCGGGAG
AGATAACGATCTGTCCTAAGGAAAGAAAAGTGCATGTGAGTGGGCAAGAGATTCAGTTAACGTACAGAGAGTTTAATTTA
TGTCAGTTGTTTGTTTCCAATCCGCAGCGTGTATTTATGAGAGATGAGTTACTTGAGAAAGTATGGGGATTTGAGTATAT
CGGAAATACGAGAGCGGTTGATATTATGGTGCAAAGATTGCGGAAGAAGCTAGGGAATAGCGGTGAATATATTAAGACCA
TTTATGGCGTTGGCTATAAACTGGATTGTTAA

Upstream 100 bases:

>100_bases
GTATTGTCGTTCAATATATGTTCGACAATACCCTCTCTTTTTTTATATGATTTTTGCTATACTAAACTGTACTATAATAC
AGGATGGAAGGAGGAGAATA

Downstream 100 bases:

>100_bases
GTGGTGATGGTATGTCTTTGAAAAGAAATATGGTGTTTGGAATCGTTGGACTGTTAATCCCGATTTTTGTTCTTTTGTAC
GCAGTTGTTTATATTACGTT

Product: DNA-binding response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MKILVVDDESSIRNLIRMQLEMEGYEVLTAADGREALERWNEGPDVLILDVMLPDTDGYELLRLFREKDRDIPVLMLTAK
SQMNDKLLGLQLGADDYVTKPFNYAELILRVKNMTRRVKKTEVTVSHEVIEAGEITICPKERKVHVSGQEIQLTYREFNL
CQLFVSNPQRVFMRDELLEKVWGFEYIGNTRAVDIMVQRLRKKLGNSGEYIKTIYGVGYKLDC

Sequences:

>Translated_223_residues
MKILVVDDESSIRNLIRMQLEMEGYEVLTAADGREALERWNEGPDVLILDVMLPDTDGYELLRLFREKDRDIPVLMLTAK
SQMNDKLLGLQLGADDYVTKPFNYAELILRVKNMTRRVKKTEVTVSHEVIEAGEITICPKERKVHVSGQEIQLTYREFNL
CQLFVSNPQRVFMRDELLEKVWGFEYIGNTRAVDIMVQRLRKKLGNSGEYIKTIYGVGYKLDC
>Mature_223_residues
MKILVVDDESSIRNLIRMQLEMEGYEVLTAADGREALERWNEGPDVLILDVMLPDTDGYELLRLFREKDRDIPVLMLTAK
SQMNDKLLGLQLGADDYVTKPFNYAELILRVKNMTRRVKKTEVTVSHEVIEAGEITICPKERKVHVSGQEIQLTYREFNL
CQLFVSNPQRVFMRDELLEKVWGFEYIGNTRAVDIMVQRLRKKLGNSGEYIKTIYGVGYKLDC

Specific function: This Protein Is A Positive Regulator For The Phosphate Regulon. Transcription Of This Operon Is Positively Regulated By Phob And Phor When Phosphate Is Limited. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=227, Percent_Identity=37.0044052863436, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI1786784, Length=226, Percent_Identity=35.3982300884956, Blast_Score=143, Evalue=9e-36,
Organism=Escherichia coli, GI1789402, Length=222, Percent_Identity=35.5855855855856, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI1789809, Length=226, Percent_Identity=34.070796460177, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1787375, Length=222, Percent_Identity=32.8828828828829, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI2367329, Length=225, Percent_Identity=36, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=34.0807174887892, Blast_Score=127, Evalue=4e-31,
Organism=Escherichia coli, GI1790860, Length=223, Percent_Identity=36.322869955157, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1790863, Length=229, Percent_Identity=34.4978165938865, Blast_Score=123, Evalue=8e-30,
Organism=Escherichia coli, GI1788394, Length=228, Percent_Identity=33.3333333333333, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI87082012, Length=224, Percent_Identity=30.8035714285714, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1790552, Length=222, Percent_Identity=31.5315315315315, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1787229, Length=228, Percent_Identity=31.5789473684211, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI145693140, Length=230, Percent_Identity=32.6086956521739, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1788550, Length=112, Percent_Identity=35.7142857142857, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1790299, Length=111, Percent_Identity=36.036036036036, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788713, Length=169, Percent_Identity=31.3609467455621, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788191, Length=111, Percent_Identity=38.7387387387387, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787487, Length=101, Percent_Identity=35.6435643564356, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790437, Length=108, Percent_Identity=36.1111111111111, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI145693157, Length=122, Percent_Identity=33.6065573770492, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25868; Mature: 25868

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVVDDESSIRNLIRMQLEMEGYEVLTAADGREALERWNEGPDVLILDVMLPDTDGYE
CEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCEEEEEEEECCCCCHH
LLRLFREKDRDIPVLMLTAKSQMNDKLLGLQLGADDYVTKPFNYAELILRVKNMTRRVKK
HHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
TEVTVSHEVIEAGEITICPKERKVHVSGQEIQLTYREFNLCQLFVSNPQRVFMRDELLEK
HEEEEHHHHCCCCCEEECCCCCEEEECCCEEEEEEECCCEEEEEECCCHHHHHHHHHHHH
VWGFEYIGNTRAVDIMVQRLRKKLGNSGEYIKTIYGVGYKLDC
HCCCHHCCCCHHHHHHHHHHHHHHCCCCCEEHHHHCCCEEECC
>Mature Secondary Structure
MKILVVDDESSIRNLIRMQLEMEGYEVLTAADGREALERWNEGPDVLILDVMLPDTDGYE
CEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCCEEEEEEEECCCCCHH
LLRLFREKDRDIPVLMLTAKSQMNDKLLGLQLGADDYVTKPFNYAELILRVKNMTRRVKK
HHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
TEVTVSHEVIEAGEITICPKERKVHVSGQEIQLTYREFNLCQLFVSNPQRVFMRDELLEK
HEEEEHHHHCCCCCEEECCCCCEEEECCCEEEEEEECCCEEEEEECCCHHHHHHHHHHHH
VWGFEYIGNTRAVDIMVQRLRKKLGNSGEYIKTIYGVGYKLDC
HCCCHHCCCCHHHHHHHHHHHHHHCCCCCEEHHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1554697 [H]