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The map label for this gene is proB [H]

Identifier: 218904105

GI number: 218904105

Start: 2825861

End: 2827105

Strand: Reverse

Name: proB [H]

Synonym: BCAH820_2989

Alternate gene names: 218904105

Gene position: 2827105-2825861 (Counterclockwise)

Preceding gene: 218904106

Following gene: 218904104

Centisome position: 53.31

GC content: 40.24

Gene sequence:

>1245_bases
ATGAAGGGCGAAGCGGTCATTTTTATTTTGCTCTTAAATAGACTAGGTGCAGCACTGATAAAAATAGAAATCGCTGCAAG
CTGTATGAATAAAAGTGTAATGTATAAAAAAACTTTGATTTTAGACTTGGGGGATATAAAAGTGAAAAAACAACGAATTG
TTGTAAAGATTGGGAGCAGTTCTTTGGCGGATAGTCACCAAGGCATTTCTAAAGAACAACTTTCAGATCATGTTGCGGCA
TTAGCTCGATTGAAAGAGGAAGGGCATGAAGTGTTGTTAATTACATCTGGGGCCGTTGCAGCAGGTTTTTCAGCTCTAGG
GTACCCTAGTAGACCTGTAACAATTAAAGGAAAACAGGCAGCTGCGGCTGTCGGACAAAGTTTGTTAATGCAGGCGTATA
CGGAGGAATTTCGTAAATACGGTATCGTTACTGCGCAACTATTGCTGACGAGAAGTGATTTTTCTAGAAAAGAACAATAT
AGTAACGCTTACGCCACTTTAGGAGAGTTACTAAACCGTTCTGCTCTTCCAATCATTAATGAAAATGATTCCATTTCTTT
AGAGGAGCTAACGTTCGGTGATAATGATATGCTGTCAGCTTTAGTAAGCGGACTTGTTTCGGCGGATATGCTGATGATTT
TTACAGATGTGAACGGTTTATATGATAAAAATCCTCAGAAAAATGAGGATGCGAAAAAATATTATTTCCTTCCTGAAGTT
ACAGAAGAAATCGCTTCTCTTGCAGGAGATGCTGGCTCAAAACTTGGGACTGGCGGAATGAAATCAAAAGTCGATGCTGC
AAAGACAGCACTCTCTCTTGGTGTCAGTGTATTTATCGGAACAGGACGTGGACAAGAGAAATTTGTAGATGTTTTGAAGG
GGAAAGGTGATGGAACGTATGTCGGAAATGCCCCTCAAAAAGAAATGAAGATGAATAAGCAATGGATCGCTTTGCATTCG
GTTGTTAGCGGACAAATTGAAATAGACGCCGGAGCAGCTACAGCTATCATTCAGCATGGCAAGAGTCTTCTTCCTGCTGG
TATTACAAATGTTTCAGGGTTTTTCCAAGTGGGCGAAGTCGTAGAAGTAATGACGCAACAAGGACGCGTGATTGGAAAAG
GACAATGCACATATAGTGCAGAGGAGCTAAGGGATGTAAAAGGTATGCAAAGCCAACAGATTCAAGCAAGAGGCGAAAGA
CATAATTATGAAGTCATTCATAGAGATTATTGGGTTTCATTTTAA

Upstream 100 bases:

>100_bases
AGTTTAGGCGTTCGCCTGCGTTATAGGCACCAAGTGGATATCTATGTATATCAATTAAGGTGGTACCGCGGAACAATGAC
CGTTTCGTCCTTTTTATTTT

Downstream 100 bases:

>100_bases
GAAGATGGATCAAAAAGGAGGAAATAAAAATGAATGAAGTGTTAGCAAAGGGAAAGAAGGCGAAAGAGATCGCTAGAGAG
CTAGTGCTTAAATCTACAGA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MKGEAVIFILLLNRLGAALIKIEIAASCMNKSVMYKKTLILDLGDIKVKKQRIVVKIGSSSLADSHQGISKEQLSDHVAA
LARLKEEGHEVLLITSGAVAAGFSALGYPSRPVTIKGKQAAAAVGQSLLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQY
SNAYATLGELLNRSALPIINENDSISLEELTFGDNDMLSALVSGLVSADMLMIFTDVNGLYDKNPQKNEDAKKYYFLPEV
TEEIASLAGDAGSKLGTGGMKSKVDAAKTALSLGVSVFIGTGRGQEKFVDVLKGKGDGTYVGNAPQKEMKMNKQWIALHS
VVSGQIEIDAGAATAIIQHGKSLLPAGITNVSGFFQVGEVVEVMTQQGRVIGKGQCTYSAEELRDVKGMQSQQIQARGER
HNYEVIHRDYWVSF

Sequences:

>Translated_414_residues
MKGEAVIFILLLNRLGAALIKIEIAASCMNKSVMYKKTLILDLGDIKVKKQRIVVKIGSSSLADSHQGISKEQLSDHVAA
LARLKEEGHEVLLITSGAVAAGFSALGYPSRPVTIKGKQAAAAVGQSLLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQY
SNAYATLGELLNRSALPIINENDSISLEELTFGDNDMLSALVSGLVSADMLMIFTDVNGLYDKNPQKNEDAKKYYFLPEV
TEEIASLAGDAGSKLGTGGMKSKVDAAKTALSLGVSVFIGTGRGQEKFVDVLKGKGDGTYVGNAPQKEMKMNKQWIALHS
VVSGQIEIDAGAATAIIQHGKSLLPAGITNVSGFFQVGEVVEVMTQQGRVIGKGQCTYSAEELRDVKGMQSQQIQARGER
HNYEVIHRDYWVSF
>Mature_414_residues
MKGEAVIFILLLNRLGAALIKIEIAASCMNKSVMYKKTLILDLGDIKVKKQRIVVKIGSSSLADSHQGISKEQLSDHVAA
LARLKEEGHEVLLITSGAVAAGFSALGYPSRPVTIKGKQAAAAVGQSLLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQY
SNAYATLGELLNRSALPIINENDSISLEELTFGDNDMLSALVSGLVSADMLMIFTDVNGLYDKNPQKNEDAKKYYFLPEV
TEEIASLAGDAGSKLGTGGMKSKVDAAKTALSLGVSVFIGTGRGQEKFVDVLKGKGDGTYVGNAPQKEMKMNKQWIALHS
VVSGQIEIDAGAATAIIQHGKSLLPAGITNVSGFFQVGEVVEVMTQQGRVIGKGQCTYSAEELRDVKGMQSQQIQARGER
HNYEVIHRDYWVSF

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=286, Percent_Identity=31.8181818181818, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI62912457, Length=284, Percent_Identity=32.0422535211268, Blast_Score=119, Evalue=5e-27,
Organism=Escherichia coli, GI1786437, Length=364, Percent_Identity=39.010989010989, Blast_Score=255, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI17569969, Length=305, Percent_Identity=33.4426229508197, Blast_Score=148, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17569967, Length=284, Percent_Identity=34.8591549295775, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6320506, Length=415, Percent_Identity=34.6987951807229, Blast_Score=209, Evalue=7e-55,
Organism=Saccharomyces cerevisiae, GI6321822, Length=401, Percent_Identity=32.9177057356608, Blast_Score=199, Evalue=7e-52,
Organism=Drosophila melanogaster, GI21357643, Length=287, Percent_Identity=35.8885017421603, Blast_Score=145, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 44746; Mature: 44746

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGEAVIFILLLNRLGAALIKIEIAASCMNKSVMYKKTLILDLGDIKVKKQRIVVKIGSS
CCCCCEEHHHHHHHHCHHHHHHHHHHHHHCCHHEEEEEEEEECCCCEEEEEEEEEEECCC
SLADSHQGISKEQLSDHVAALARLKEEGHEVLLITSGAVAAGFSALGYPSRPVTIKGKQA
CCCHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCHHHCCCCCCCEEEECCHH
AAAVGQSLLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQYSNAYATLGELLNRSALPIIN
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEC
ENDSISLEELTFGDNDMLSALVSGLVSADMLMIFTDVNGLYDKNPQKNEDAKKYYFLPEV
CCCCCEEEEEECCCHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCEEEECHHH
TEEIASLAGDAGSKLGTGGMKSKVDAAKTALSLGVSVFIGTGRGQEKFVDVLKGKGDGTY
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHCCCCCCC
VGNAPQKEMKMNKQWIALHSVVSGQIEIDAGAATAIIQHGKSLLPAGITNVSGFFQVGEV
CCCCCHHHHHHCHHHHHHHHHHCCEEEECCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
VEVMTQQGRVIGKGQCTYSAEELRDVKGMQSQQIQARGERHNYEVIHRDYWVSF
HHHHHHCCCEEECCCCCCCHHHHHHHHCCHHHHHHHCCCCCCEEEEEEEEEECC
>Mature Secondary Structure
MKGEAVIFILLLNRLGAALIKIEIAASCMNKSVMYKKTLILDLGDIKVKKQRIVVKIGSS
CCCCCEEHHHHHHHHCHHHHHHHHHHHHHCCHHEEEEEEEEECCCCEEEEEEEEEEECCC
SLADSHQGISKEQLSDHVAALARLKEEGHEVLLITSGAVAAGFSALGYPSRPVTIKGKQA
CCCHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCHHHCCCCCCCEEEECCHH
AAAVGQSLLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQYSNAYATLGELLNRSALPIIN
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEC
ENDSISLEELTFGDNDMLSALVSGLVSADMLMIFTDVNGLYDKNPQKNEDAKKYYFLPEV
CCCCCEEEEEECCCHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCEEEECHHH
TEEIASLAGDAGSKLGTGGMKSKVDAAKTALSLGVSVFIGTGRGQEKFVDVLKGKGDGTY
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHCCCCCCC
VGNAPQKEMKMNKQWIALHSVVSGQIEIDAGAATAIIQHGKSLLPAGITNVSGFFQVGEV
CCCCCHHHHHHCHHHHHHHHHHCCEEEECCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
VEVMTQQGRVIGKGQCTYSAEELRDVKGMQSQQIQARGERHNYEVIHRDYWVSF
HHHHHHCCCEEECCCCCCCHHHHHHHHCCHHHHHHHCCCCCCEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]