Click here to switch to the map view.

The map label for this gene is aspA2 [H]

Identifier: 218902964

GI number: 218902964

Start: 1741312

End: 1742751

Strand: Direct

Name: aspA2 [H]

Synonym: BCAH820_1847

Alternate gene names: 218902964

Gene position: 1741312-1742751 (Clockwise)

Preceding gene: 218902963

Following gene: 218902965

Centisome position: 32.84

GC content: 38.75

Gene sequence:

>1440_bases
ATGGCTACATTAACTGAAGTTAAAAATGGTGTTCGAATTGAAAAAGATTTTTTAGGTGAAAAAGAAGTACCAAACTATGC
ATACTACGGCGTACAAACAATGCGTGCAGTTGAAAACTTCCCAATTACAGGATACAAAATCCATGAAGGTTTAATTAGAG
CGTTCGCAGTTGTAAAAAAAGCTGCAGCGCTTGCGAATACAGATGTAGGAAGATTGGAATTGAACAAGGGCGGCGCGATC
GCAGAAGCTGCTCAAGAAATTCTTGATGGAAAATGGCATGATCATTTCATCGTAGATCCAATCCAAGGCGGAGCAGGTAC
TTCAATGAACATGAATGCAAATGAAGTCATTGCCAATCGTGCTCTTGAATTATTAGGGATGGAAAAGGGAGACTATCATT
ATATTAGCCCAAATAGTCATGTAAACATGGCGCAATCAACAAATGATGCATTCCCAACGGCGATTCATATCGCAACATTA
AACGCATTAGAAGGTTTATTACAAACGATGGGTTATATGCACGATGTATTTGAATTAAAAGCAGAACAGTTCGATCATGT
GATTAAAATGGGTCGTACACATTTACAAGACGCTGTACCAATTCGTCTTGGACAAGAATTTAAAGCGTACTCTCGTGTAC
TTGAACGTGATATGAAACGAATTCAGCAATCACGTCAACACTTATATGAAGTGAACATGGGGGCAACTGCAGTTGGTACA
GGCTTAAATGCAGATCCAGAATATATTGAAGCAGTTGTAAAACATTTAGCTGCAATTAGTGAACTACCACTTGTTGGTGC
AGAAGATTTAGTAGATGCGACGCAAAATACGGATGCTTATACTGAAGTATCTGCAGCACTTAAAGTATGTATGATGAATA
TGTCTAAAATTGCGAATGACCTTCGCTTAATGGCATCAGGTCCACGTGTTGGCTTAGCGGAAATTATGCTACCTGCTCGT
CAACCAGGTTCTTCTATTATGCCAGGGAAAGTAAACCCTGTTATGCCAGAAGTAATTAATCAAATTGCATTCCAAGTAAT
TGGTAACGACCATACAATTTGTCTTGCTTCAGAAGCAGGTCAATTAGAATTAAACGTGATGGAACCAGTACTTGTTTTCA
ACTTACTACAATCAATTAGCATTATGAATAACGGTTTCCGTGCCTTTACAGATAATTGCTTAAAAGGAATTGAAGCGAAT
GAAGATCGCTTAAAAGAGTATGTTGAGAAGAGTGTAGGAATTATTACAGCCGTGAACCCTCATATCGGTTATGAAGCAGC
AGCTCGCGTTGCGAAAGAAGCAATCGCGACAGGGCAATCCGTTCGAGAACTTTGTGTGAAAAATGGTGTATTGTCACAAG
AAGATTTAGAGTTAATTCTAGATCCATTTGAAATGACACACCCAGGGATTGCAGGAGCAACTCTTTTAAAGAAAAATTAA

Upstream 100 bases:

>100_bases
GAAGTGGGAAGGCGAATTCGATAATGAGAAAGCAAAACAATATGTAGAAACAGTCAAAGAAACAAAAGCAGCATAGGAAA
TCGTTAAGGAGTGAATAATC

Downstream 100 bases:

>100_bases
AAGAAGAGGGGGACAAGCCCCTCTTTTTTGTTTACAATATAGAAATGCTATATATGAATAGAGGTAGGGATGTTATGAGC
AAGTTTACAGTAGCTTCTAA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MATLTEVKNGVRIEKDFLGEKEVPNYAYYGVQTMRAVENFPITGYKIHEGLIRAFAVVKKAAALANTDVGRLELNKGGAI
AEAAQEILDGKWHDHFIVDPIQGGAGTSMNMNANEVIANRALELLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATL
NALEGLLQTMGYMHDVFELKAEQFDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGT
GLNADPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLMASGPRVGLAEIMLPAR
QPGSSIMPGKVNPVMPEVINQIAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEAN
EDRLKEYVEKSVGIITAVNPHIGYEAAARVAKEAIATGQSVRELCVKNGVLSQEDLELILDPFEMTHPGIAGATLLKKN

Sequences:

>Translated_479_residues
MATLTEVKNGVRIEKDFLGEKEVPNYAYYGVQTMRAVENFPITGYKIHEGLIRAFAVVKKAAALANTDVGRLELNKGGAI
AEAAQEILDGKWHDHFIVDPIQGGAGTSMNMNANEVIANRALELLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATL
NALEGLLQTMGYMHDVFELKAEQFDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGT
GLNADPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLMASGPRVGLAEIMLPAR
QPGSSIMPGKVNPVMPEVINQIAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEAN
EDRLKEYVEKSVGIITAVNPHIGYEAAARVAKEAIATGQSVRELCVKNGVLSQEDLELILDPFEMTHPGIAGATLLKKN
>Mature_478_residues
ATLTEVKNGVRIEKDFLGEKEVPNYAYYGVQTMRAVENFPITGYKIHEGLIRAFAVVKKAAALANTDVGRLELNKGGAIA
EAAQEILDGKWHDHFIVDPIQGGAGTSMNMNANEVIANRALELLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATLN
ALEGLLQTMGYMHDVFELKAEQFDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGTG
LNADPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLMASGPRVGLAEIMLPARQ
PGSSIMPGKVNPVMPEVINQIAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEANE
DRLKEYVEKSVGIITAVNPHIGYEAAARVAKEAIATGQSVRELCVKNGVLSQEDLELILDPFEMTHPGIAGATLLKKN

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=464, Percent_Identity=43.3189655172414, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI68303547, Length=245, Percent_Identity=29.7959183673469, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI31541964, Length=244, Percent_Identity=29.9180327868852, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI68303542, Length=244, Percent_Identity=29.9180327868852, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI87082375, Length=465, Percent_Identity=49.8924731182796, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1787896, Length=465, Percent_Identity=42.3655913978495, Blast_Score=374, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17553882, Length=461, Percent_Identity=44.0347071583514, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=42.2018348623853, Blast_Score=274, Evalue=9e-74,
Organism=Saccharomyces cerevisiae, GI6324993, Length=468, Percent_Identity=41.6666666666667, Blast_Score=376, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24640177, Length=463, Percent_Identity=42.548596112311, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24640179, Length=463, Percent_Identity=42.548596112311, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI78710009, Length=462, Percent_Identity=41.5584415584416, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24662684, Length=463, Percent_Identity=42.3326133909287, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24583245, Length=463, Percent_Identity=38.2289416846652, Blast_Score=335, Evalue=4e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 52308; Mature: 52177

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLTEVKNGVRIEKDFLGEKEVPNYAYYGVQTMRAVENFPITGYKIHEGLIRAFAVVKK
CCCHHHHHCCCEECHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
AAALANTDVGRLELNKGGAIAEAAQEILDGKWHDHFIVDPIQGGAGTSMNMNANEVIANR
HHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHH
ALELLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATLNALEGLLQTMGYMHDVFELK
HHHHHCCCCCCEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEQFDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGT
HHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
GLNADPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIAND
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LRLMASGPRVGLAEIMLPARQPGSSIMPGKVNPVMPEVINQIAFQVIGNDHTICLASEAG
HHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCC
QLELNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEANEDRLKEYVEKSVGIITAVNP
CEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEECCC
HIGYEAAARVAKEAIATGQSVRELCVKNGVLSQEDLELILDPFEMTHPGIAGATLLKKN
CCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHCCCHHHCCCCCCCCHHCCCC
>Mature Secondary Structure 
ATLTEVKNGVRIEKDFLGEKEVPNYAYYGVQTMRAVENFPITGYKIHEGLIRAFAVVKK
CCHHHHHCCCEECHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
AAALANTDVGRLELNKGGAIAEAAQEILDGKWHDHFIVDPIQGGAGTSMNMNANEVIANR
HHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHH
ALELLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATLNALEGLLQTMGYMHDVFELK
HHHHHCCCCCCEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEQFDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGT
HHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
GLNADPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIAND
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LRLMASGPRVGLAEIMLPARQPGSSIMPGKVNPVMPEVINQIAFQVIGNDHTICLASEAG
HHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCC
QLELNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEANEDRLKEYVEKSVGIITAVNP
CEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEECCC
HIGYEAAARVAKEAIATGQSVRELCVKNGVLSQEDLELILDPFEMTHPGIAGATLLKKN
CCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHCCCHHHCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]