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The map label for this gene is malS [H]

Identifier: 218902965

GI number: 218902965

Start: 1742826

End: 1744538

Strand: Direct

Name: malS [H]

Synonym: BCAH820_1848

Alternate gene names: 218902965

Gene position: 1742826-1744538 (Clockwise)

Preceding gene: 218902964

Following gene: 218902966

Centisome position: 32.87

GC content: 39.64

Gene sequence:

>1713_bases
ATGAGCAAGTTTACAGTAGCTTCTAATGGTTCATTAGAAACGACATTAAGAGGAGTAGAAGTATTATCAACACCACTTTT
AAATAAAGGGGTCGCTTTCACGCAAGAAGAAAGAGAAGAATTAGGTTTAAAAGGGTTATTACCGCCAGCAGTTTTAACAT
TGGAAGAACAAGCACGCCGAGCATACGAACAATTTTGTTCTCAGCCGGATGATTTATTAAAGAATGTATACTTAACAGCG
TTACATGATCGAAATGAAGTGTTATTTTATCGCATTTTAACAGATCATTTACGCGAAATGTTACCAATCGTATATACACC
AACTGTTGGTGTAGCAATCCAAAGATATAGTCATGAATACCGTAAACCACGTGGTGTGTATTTATCAATTAACGATCCGT
CAGGTATTGAAGAAGCATTCGCCAATATCGGTGCAACAGCAGAAAATATTGATTTAGTCGTTGTTACAGATGGAGAAGGT
ATACTTGGAATTGGTGACTGGGGCGTTGGTGGTATTAACATCGCAATCGGAAAATTAGCTGTTTATACAGCCGCAGTTGG
AATCAATCCTAGCCGCGTATTACCGGTAATTTTAGATGTAGGTACAAATCGTGAAGAATTATTAGACAATCCATTTTATA
TTGGAAATCGTCATCCTCGTATAACAGGTGAAGCTTACGATGAATTTATTGATACATTTGTACAAGCGGTAAATAAGCAA
TTCCCGAAAGCACTTTTACATTGGGAAGACTTCAGTTCTCGTAATGCACGAAAAATTTTAGATAAATATCGTCATGATAT
TTGTACGTTTAATGATGATATTCAAGGTACAGGTGCAGTTTCGCTTGCGGCTGTATTATCGGCAGTAAAAGCTTCTGGTG
TACCGCTAAGTGAACACCGCGTTGTTGTGTTTGGTGCTGGTACGGCTGGAATCGGTATCGCAGATCAAGTTAGAGATGCG
ATGGTACGCGTCGGCGTATCAGAAGAAGAATCATATAAGCGTTTCTGGTGTATTGATCGTAACGGATTAGTTACAGATAA
CATGGAAGATCTTCTTGATTTCCAAATTCCATATGCAAGAAAAGAAGCGGAAGTAAGTGAGTGGAAAGAAAATGGTGTGA
TCGGGCTTGCTGAAGTTGTGAAACATGTGAAACCGACAATTTTAATTGGTACATCAACTGTTGCAGGCGCATTTAAAGAA
GAAATTATTAAAGAAATGGCTTCTCACGTAGAAAGACCAATCATTTTACCGATGTCGAATCCGACGCCACTTGCTGAAGC
GAAACCAGCCGATTTAATCGAGTGGACAGAAGGAAGAGCACTAGTTGCAACAGGAAGTCCATTTGAACCAGTTACATATA
ACGGTGTAACGTATGTAATTGGGCAATCAAATAATGCACTTATTTTCCCAGGTCTTGGTCTTGGAACAATTGTCGTACGT
GCAAGCGTCATGACAGATGGAATGTTCGCAGCAGCAGCTGAAGCAGTTGCAAGTATGGTGGACACAAGTCAGCCAGGAGC
ACCTATTTTGCCAGAAGTTGAAGAGTTACGTAATATCTCGGAAATGGTAGCAATTGAAGTAGCGAAAGTTGCTGTTGCAG
AAGGTGTTGCTAGAGAGAACTTAAGTGATAACGATATTAAGATTGCAGTGAAAGAAGCAATCTGGGAGCCTGAGTATCGC
CAAATAAAAGCGGTAGAAAAGGTTACAATATAG

Upstream 100 bases:

>100_bases
GAGCAACTCTTTTAAAGAAAAATTAAAAGAAGAGGGGGACAAGCCCCTCTTTTTTGTTTACAATATAGAAATGCTATATA
TGAATAGAGGTAGGGATGTT

Downstream 100 bases:

>100_bases
AAATAAAGCCCGTTTATAATATAAGCGGGCTTTCACTTTTATTACTGAAAAAAAGAGTATAGGAAGTGGCACCTTTGAAT
TGGAATCAATTATGGAATAA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME 3 [H]

Number of amino acids: Translated: 570; Mature: 569

Protein sequence:

>570_residues
MSKFTVASNGSLETTLRGVEVLSTPLLNKGVAFTQEEREELGLKGLLPPAVLTLEEQARRAYEQFCSQPDDLLKNVYLTA
LHDRNEVLFYRILTDHLREMLPIVYTPTVGVAIQRYSHEYRKPRGVYLSINDPSGIEEAFANIGATAENIDLVVVTDGEG
ILGIGDWGVGGINIAIGKLAVYTAAVGINPSRVLPVILDVGTNREELLDNPFYIGNRHPRITGEAYDEFIDTFVQAVNKQ
FPKALLHWEDFSSRNARKILDKYRHDICTFNDDIQGTGAVSLAAVLSAVKASGVPLSEHRVVVFGAGTAGIGIADQVRDA
MVRVGVSEEESYKRFWCIDRNGLVTDNMEDLLDFQIPYARKEAEVSEWKENGVIGLAEVVKHVKPTILIGTSTVAGAFKE
EIIKEMASHVERPIILPMSNPTPLAEAKPADLIEWTEGRALVATGSPFEPVTYNGVTYVIGQSNNALIFPGLGLGTIVVR
ASVMTDGMFAAAAEAVASMVDTSQPGAPILPEVEELRNISEMVAIEVAKVAVAEGVARENLSDNDIKIAVKEAIWEPEYR
QIKAVEKVTI

Sequences:

>Translated_570_residues
MSKFTVASNGSLETTLRGVEVLSTPLLNKGVAFTQEEREELGLKGLLPPAVLTLEEQARRAYEQFCSQPDDLLKNVYLTA
LHDRNEVLFYRILTDHLREMLPIVYTPTVGVAIQRYSHEYRKPRGVYLSINDPSGIEEAFANIGATAENIDLVVVTDGEG
ILGIGDWGVGGINIAIGKLAVYTAAVGINPSRVLPVILDVGTNREELLDNPFYIGNRHPRITGEAYDEFIDTFVQAVNKQ
FPKALLHWEDFSSRNARKILDKYRHDICTFNDDIQGTGAVSLAAVLSAVKASGVPLSEHRVVVFGAGTAGIGIADQVRDA
MVRVGVSEEESYKRFWCIDRNGLVTDNMEDLLDFQIPYARKEAEVSEWKENGVIGLAEVVKHVKPTILIGTSTVAGAFKE
EIIKEMASHVERPIILPMSNPTPLAEAKPADLIEWTEGRALVATGSPFEPVTYNGVTYVIGQSNNALIFPGLGLGTIVVR
ASVMTDGMFAAAAEAVASMVDTSQPGAPILPEVEELRNISEMVAIEVAKVAVAEGVARENLSDNDIKIAVKEAIWEPEYR
QIKAVEKVTI
>Mature_569_residues
SKFTVASNGSLETTLRGVEVLSTPLLNKGVAFTQEEREELGLKGLLPPAVLTLEEQARRAYEQFCSQPDDLLKNVYLTAL
HDRNEVLFYRILTDHLREMLPIVYTPTVGVAIQRYSHEYRKPRGVYLSINDPSGIEEAFANIGATAENIDLVVVTDGEGI
LGIGDWGVGGINIAIGKLAVYTAAVGINPSRVLPVILDVGTNREELLDNPFYIGNRHPRITGEAYDEFIDTFVQAVNKQF
PKALLHWEDFSSRNARKILDKYRHDICTFNDDIQGTGAVSLAAVLSAVKASGVPLSEHRVVVFGAGTAGIGIADQVRDAM
VRVGVSEEESYKRFWCIDRNGLVTDNMEDLLDFQIPYARKEAEVSEWKENGVIGLAEVVKHVKPTILIGTSTVAGAFKEE
IIKEMASHVERPIILPMSNPTPLAEAKPADLIEWTEGRALVATGSPFEPVTYNGVTYVIGQSNNALIFPGLGLGTIVVRA
SVMTDGMFAAAAEAVASMVDTSQPGAPILPEVEELRNISEMVAIEVAKVAVAEGVARENLSDNDIKIAVKEAIWEPEYRQ
IKAVEKVTI

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=546, Percent_Identity=42.3076923076923, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI62420880, Length=546, Percent_Identity=42.3076923076923, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI239049447, Length=546, Percent_Identity=42.3076923076923, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI4505143, Length=554, Percent_Identity=40.7942238267148, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI4505145, Length=548, Percent_Identity=39.7810218978102, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI270265879, Length=459, Percent_Identity=42.483660130719, Blast_Score=360, Evalue=1e-99,
Organism=Escherichia coli, GI87081919, Length=555, Percent_Identity=46.8468468468468, Blast_Score=509, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI17537199, Length=552, Percent_Identity=43.1159420289855, Blast_Score=435, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6322823, Length=578, Percent_Identity=45.3287197231834, Blast_Score=508, Evalue=1e-144,
Organism=Drosophila melanogaster, GI281362672, Length=550, Percent_Identity=40.5454545454545, Blast_Score=422, Evalue=1e-118,
Organism=Drosophila melanogaster, GI281362674, Length=550, Percent_Identity=40.5454545454545, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI21356279, Length=550, Percent_Identity=40.5454545454545, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24646386, Length=527, Percent_Identity=39.8481973434535, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24646388, Length=527, Percent_Identity=39.8481973434535, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI78707242, Length=543, Percent_Identity=35.7274401473296, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI78707238, Length=543, Percent_Identity=35.7274401473296, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI281363505, Length=563, Percent_Identity=33.9253996447602, Blast_Score=358, Evalue=5e-99,
Organism=Drosophila melanogaster, GI78707236, Length=563, Percent_Identity=33.9253996447602, Blast_Score=358, Evalue=5e-99,
Organism=Drosophila melanogaster, GI281363503, Length=536, Percent_Identity=34.5149253731343, Blast_Score=354, Evalue=9e-98,
Organism=Drosophila melanogaster, GI78707240, Length=519, Percent_Identity=36.4161849710983, Blast_Score=353, Evalue=3e-97,
Organism=Drosophila melanogaster, GI78707232, Length=537, Percent_Identity=34.6368715083799, Blast_Score=352, Evalue=6e-97,
Organism=Drosophila melanogaster, GI19922384, Length=549, Percent_Identity=28.7795992714025, Blast_Score=251, Evalue=7e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 62213; Mature: 62082

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFTVASNGSLETTLRGVEVLSTPLLNKGVAFTQEEREELGLKGLLPPAVLTLEEQARR
CCCEEECCCCCHHHHHHHHHHHHCCHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHH
AYEQFCSQPDDLLKNVYLTALHDRNEVLFYRILTDHLREMLPIVYTPTVGVAIQRYSHEY
HHHHHHCCHHHHHHHHEEEEEECCCCEEEHHHHHHHHHHHCCEEECCCHHHHHHHHHHHH
RKPRGVYLSINDPSGIEEAFANIGATAENIDLVVVTDGEGILGIGDWGVGGINIAIGKLA
CCCCCEEEEECCCCCHHHHHHHCCCCCCCEEEEEEECCCCEEEECCCCCCCEEEEHHHHE
VYTAAVGINPSRVLPVILDVGTNREELLDNPFYIGNRHPRITGEAYDEFIDTFVQAVNKQ
EHHEEECCCCHHEEEEEEECCCCHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHH
FPKALLHWEDFSSRNARKILDKYRHDICTFNDDIQGTGAVSLAAVLSAVKASGVPLSEHR
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCE
VVVFGAGTAGIGIADQVRDAMVRVGVSEEESYKRFWCIDRNGLVTDNMEDLLDFQIPYAR
EEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCC
KEAEVSEWKENGVIGLAEVVKHVKPTILIGTSTVAGAFKEEIIKEMASHVERPIILPMSN
CCCHHHHHHHCCCEEHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEECCC
PTPLAEAKPADLIEWTEGRALVATGSPFEPVTYNGVTYVIGQSNNALIFPGLGLGTIVVR
CCCCCCCCCCCCEEECCCCEEEECCCCCCCEEECCEEEEEECCCCEEEECCCCHHHHHHH
ASVMTDGMFAAAAEAVASMVDTSQPGAPILPEVEELRNISEMVAIEVAKVAVAEGVAREN
HHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LSDNDIKIAVKEAIWEPEYRQIKAVEKVTI
CCCCCEEEEEHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SKFTVASNGSLETTLRGVEVLSTPLLNKGVAFTQEEREELGLKGLLPPAVLTLEEQARR
CCEEECCCCCHHHHHHHHHHHHCCHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHH
AYEQFCSQPDDLLKNVYLTALHDRNEVLFYRILTDHLREMLPIVYTPTVGVAIQRYSHEY
HHHHHHCCHHHHHHHHEEEEEECCCCEEEHHHHHHHHHHHCCEEECCCHHHHHHHHHHHH
RKPRGVYLSINDPSGIEEAFANIGATAENIDLVVVTDGEGILGIGDWGVGGINIAIGKLA
CCCCCEEEEECCCCCHHHHHHHCCCCCCCEEEEEEECCCCEEEECCCCCCCEEEEHHHHE
VYTAAVGINPSRVLPVILDVGTNREELLDNPFYIGNRHPRITGEAYDEFIDTFVQAVNKQ
EHHEEECCCCHHEEEEEEECCCCHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHH
FPKALLHWEDFSSRNARKILDKYRHDICTFNDDIQGTGAVSLAAVLSAVKASGVPLSEHR
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCE
VVVFGAGTAGIGIADQVRDAMVRVGVSEEESYKRFWCIDRNGLVTDNMEDLLDFQIPYAR
EEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCC
KEAEVSEWKENGVIGLAEVVKHVKPTILIGTSTVAGAFKEEIIKEMASHVERPIILPMSN
CCCHHHHHHHCCCEEHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEECCC
PTPLAEAKPADLIEWTEGRALVATGSPFEPVTYNGVTYVIGQSNNALIFPGLGLGTIVVR
CCCCCCCCCCCCEEECCCCEEEECCCCCCCEEECCEEEEEECCCCEEEECCCCHHHHHHH
ASVMTDGMFAAAAEAVASMVDTSQPGAPILPEVEELRNISEMVAIEVAKVAVAEGVAREN
HHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LSDNDIKIAVKEAIWEPEYRQIKAVEKVTI
CCCCCEEEEEHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]