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The map label for this gene is leuC [H]

Identifier: 218902610

GI number: 218902610

Start: 1411912

End: 1413306

Strand: Direct

Name: leuC [H]

Synonym: BCAH820_1493

Alternate gene names: 218902610

Gene position: 1411912-1413306 (Clockwise)

Preceding gene: 218902609

Following gene: 218902611

Centisome position: 26.63

GC content: 41.65

Gene sequence:

>1395_bases
ATGGGGAAAAGGTTGCTAGATAAGCTTTGGGAAAGACACGTAGTTACGACCAACGAAAATGGATTGGATTTATTATATAT
CGATCTGCATCTTGTTCATGAAGTAACGTCACCGCAAGCCTTTGAAGGCTTGCGGCTTACAAATCGAACGGTACGCAGAC
CAGATTTAACATTCGCAACGATGGATCATAATATTCCAACGAAAGATGTTTGGAATATTACCGATCGCATTGCGAAGCAG
CAACTAGATATGCTTCGGGAAAATTGTAAACAATTTCAGGTGCCGTTAGCGGATATCGGGGATGAAGAGCAAGGGATCGT
TCATGTTATCGGACCAGAACTTGGGCTCACGCAGCCAGGAAAAACAATTGTTTGTGGTGATAGTCATACAGCAACGCACG
GTGCGTTTGGCGCGCTAGCATTTGGCATTGGTACGAGTGAAGTGGAACATGTATTGGCAACGCAAACGTTGTGGCAAAGA
AAACCGAAAGCGATGGGAATTGAGTTAAAAGGAAAATTACAGAAAGGCGTTTACGCAAAAGATATTATTTTGCATCTCCT
TTCAAAGTACGGTGTAGCAGTTGGAACTGGATACGTAATGGAATTTTACGGAGAGACGATTGGAACCATGGAGATGGAAG
AGAGAATGACGCTTTGTAATATGGCGATTGAAGGAGGAGCAAAAGCGGGTATTATCGCACCAGATGAAAAAACATTTGCT
TATGTAAAAGGACGTAAATATGCACCGAGAGACTATGAAACTTTCGAGAAGAAATGGTTTGAACTGTATACAGATGCAGA
TGCAATTTATGATTTACATATTTCGATAGATGTTACGGATTTAGCGCCGTATGTTACGTGGGGAACGAACCCGAGTATGG
GAGTTCGTATTGATGAGAAATTGCCAGAAAAGCATGATGTAAATGACGAAAGAGCATTCTCTTATATGGGATTAATCCCT
GGACAAAGCACGTATGACATTCCAGTTCAGCATGTCTTCATTGGATCTTGTACAAATTCTAGATTATCTGATTTAGAAAT
TGCTGCATCTGTTGTGAAAGGGAGAAAGGTAAAAGAAGGTGTGCGAGCACTCGTTGTACCTGGATCGAAAAGAGTAAGGG
ATGCGGCGATGCAAAAAGGACTACATCACATATTTGAAGAAGCTGGATTTGAATGGAGAGAACCTGGATGTTCCATGTGT
CTTGGAATGAATCCAGATCAAGTACCTGAAGGAGAGCATTGTGCTTCAACTTCAAATCGTAATTTTGAAGGAAGACAAGG
AAAAGGAGCACGAACGCATTTAGTTAGCCCAGCAATGGCGGCAGCAGCTGCGTTATATGGTCATTTTGTTGATATTAGAA
AGGAGAGTTATGATGGAGCCATTTCGTATTCATAA

Upstream 100 bases:

>100_bases
AATGTACAGCAGATATCGGGGGGACTGAAACGACAACTTCATTTACAAAGGCAGTTATGCAAGAAATGGAAGAACAAGCG
CTAGTAGGGAGAGGAAGATA

Downstream 100 bases:

>100_bases
AGGTACTGCCGCAGTACTAATGAATGATAACATTGATACGGATCAAATTATTCCGAAGCAATATTTAAAGAGAATTGAAA
GAACTGGGTTTGGAAAGTTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MGKRLLDKLWERHVVTTNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRTVRRPDLTFATMDHNIPTKDVWNITDRIAKQ
QLDMLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPGKTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQR
KPKAMGIELKGKLQKGVYAKDIILHLLSKYGVAVGTGYVMEFYGETIGTMEMEERMTLCNMAIEGGAKAGIIAPDEKTFA
YVKGRKYAPRDYETFEKKWFELYTDADAIYDLHISIDVTDLAPYVTWGTNPSMGVRIDEKLPEKHDVNDERAFSYMGLIP
GQSTYDIPVQHVFIGSCTNSRLSDLEIAASVVKGRKVKEGVRALVVPGSKRVRDAAMQKGLHHIFEEAGFEWREPGCSMC
LGMNPDQVPEGEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAALYGHFVDIRKESYDGAISYS

Sequences:

>Translated_464_residues
MGKRLLDKLWERHVVTTNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRTVRRPDLTFATMDHNIPTKDVWNITDRIAKQ
QLDMLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPGKTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQR
KPKAMGIELKGKLQKGVYAKDIILHLLSKYGVAVGTGYVMEFYGETIGTMEMEERMTLCNMAIEGGAKAGIIAPDEKTFA
YVKGRKYAPRDYETFEKKWFELYTDADAIYDLHISIDVTDLAPYVTWGTNPSMGVRIDEKLPEKHDVNDERAFSYMGLIP
GQSTYDIPVQHVFIGSCTNSRLSDLEIAASVVKGRKVKEGVRALVVPGSKRVRDAAMQKGLHHIFEEAGFEWREPGCSMC
LGMNPDQVPEGEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAALYGHFVDIRKESYDGAISYS
>Mature_463_residues
GKRLLDKLWERHVVTTNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRTVRRPDLTFATMDHNIPTKDVWNITDRIAKQQ
LDMLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPGKTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQRK
PKAMGIELKGKLQKGVYAKDIILHLLSKYGVAVGTGYVMEFYGETIGTMEMEERMTLCNMAIEGGAKAGIIAPDEKTFAY
VKGRKYAPRDYETFEKKWFELYTDADAIYDLHISIDVTDLAPYVTWGTNPSMGVRIDEKLPEKHDVNDERAFSYMGLIPG
QSTYDIPVQHVFIGSCTNSRLSDLEIAASVVKGRKVKEGVRALVVPGSKRVRDAAMQKGLHHIFEEAGFEWREPGCSMCL
GMNPDQVPEGEHCASTSNRNFEGRQGKGARTHLVSPAMAAAAALYGHFVDIRKESYDGAISYS

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=355, Percent_Identity=26.4788732394366, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI8659555, Length=386, Percent_Identity=25.9067357512953, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI41352693, Length=377, Percent_Identity=25.9946949602122, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=58.7096774193548, Blast_Score=523, Evalue=1e-150,
Organism=Escherichia coli, GI1787531, Length=363, Percent_Identity=27.5482093663912, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI87081781, Length=343, Percent_Identity=24.4897959183673, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI2367097, Length=337, Percent_Identity=25.2225519287834, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=359, Percent_Identity=30.9192200557103, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32564738, Length=359, Percent_Identity=31.7548746518106, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI25149342, Length=305, Percent_Identity=30.4918032786885, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17568399, Length=429, Percent_Identity=26.8065268065268, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=55.7203389830508, Blast_Score=491, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6320440, Length=414, Percent_Identity=28.2608695652174, Blast_Score=149, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6323335, Length=356, Percent_Identity=29.2134831460674, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6322261, Length=404, Percent_Identity=29.2079207920792, Blast_Score=136, Evalue=5e-33,
Organism=Drosophila melanogaster, GI281365315, Length=393, Percent_Identity=27.4809160305344, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI17864292, Length=393, Percent_Identity=27.4809160305344, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI161076999, Length=384, Percent_Identity=27.34375, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI28571643, Length=391, Percent_Identity=28.1329923273657, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24645686, Length=380, Percent_Identity=27.6315789473684, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17137564, Length=380, Percent_Identity=25.7894736842105, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 51547; Mature: 51416

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKRLLDKLWERHVVTTNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRTVRRPDLTFAT
CCHHHHHHHHHCCEEEECCCCCEEEEEEEEEHHHHCCCHHHCCCCCCCCCCCCCCCEEEE
MDHNIPTKDVWNITDRIAKQQLDMLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPG
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCCCCCCC
KTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQRKPKAMGIELKGKLQKGVYAK
CEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECHHHCCCHHH
DIILHLLSKYGVAVGTGYVMEFYGETIGTMEMEERMTLCNMAIEGGAKAGIIAPDEKTFA
HHHHHHHHHCCCCCCCHHHHHHHCCHHCCHHHHHHHHHHHHHHCCCCCCCEECCCCCEEE
YVKGRKYAPRDYETFEKKWFELYTDADAIYDLHISIDVTDLAPYVTWGTNPSMGVRIDEK
EEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEHHCCCEEEECCCCCCCEEEHHH
LPEKHDVNDERAFSYMGLIPGQSTYDIPVQHVFIGSCTNSRLSDLEIAASVVKGRKVKEG
CCCCCCCCHHHHHHEEECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCHHHCC
VRALVVPGSKRVRDAAMQKGLHHIFEEAGFEWREPGCSMCLGMNPDQVPEGEHCASTSNR
CEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCHHCCCCCC
NFEGRQGKGARTHLVSPAMAAAAALYGHFVDIRKESYDGAISYS
CCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEECC
>Mature Secondary Structure 
GKRLLDKLWERHVVTTNENGLDLLYIDLHLVHEVTSPQAFEGLRLTNRTVRRPDLTFAT
CHHHHHHHHHCCEEEECCCCCEEEEEEEEEHHHHCCCHHHCCCCCCCCCCCCCCCEEEE
MDHNIPTKDVWNITDRIAKQQLDMLRENCKQFQVPLADIGDEEQGIVHVIGPELGLTQPG
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEECCCCCCCCCC
KTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLWQRKPKAMGIELKGKLQKGVYAK
CEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECHHHCCCHHH
DIILHLLSKYGVAVGTGYVMEFYGETIGTMEMEERMTLCNMAIEGGAKAGIIAPDEKTFA
HHHHHHHHHCCCCCCCHHHHHHHCCHHCCHHHHHHHHHHHHHHCCCCCCCEECCCCCEEE
YVKGRKYAPRDYETFEKKWFELYTDADAIYDLHISIDVTDLAPYVTWGTNPSMGVRIDEK
EEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEEEHHCCCEEEECCCCCCCEEEHHH
LPEKHDVNDERAFSYMGLIPGQSTYDIPVQHVFIGSCTNSRLSDLEIAASVVKGRKVKEG
CCCCCCCCHHHHHHEEECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCHHHCC
VRALVVPGSKRVRDAAMQKGLHHIFEEAGFEWREPGCSMCLGMNPDQVPEGEHCASTSNR
CEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCHHCCCCCC
NFEGRQGKGARTHLVSPAMAAAAALYGHFVDIRKESYDGAISYS
CCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA