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The map label for this gene is leuB [H]

Identifier: 218902609

GI number: 218902609

Start: 1410848

End: 1411912

Strand: Direct

Name: leuB [H]

Synonym: BCAH820_1492

Alternate gene names: 218902609

Gene position: 1410848-1411912 (Clockwise)

Preceding gene: 218902608

Following gene: 218902610

Centisome position: 26.61

GC content: 42.63

Gene sequence:

>1065_bases
TTGGAAAAACGTATCGTTTGTTTAGCTGGTGATGGTGTTGGCCCAGAAGTTATGGAAAGTGCGAAGGAAGTATTGCATAT
GGTAGAGAGGCTATATGGACATCATTTTCATTTGCAAGATGAGCATTTTGGTGGTGTTGCTATCGATTTAACGGGGCAAC
CATTACCACAACGAACACTTGCAGCTTGTTTAGCAAGTGATGCGGTTTTACTAGGCGCAGTTGGTGGACCAAGGTGGGAT
GGTGCGAAAGAAAGGCCAGAGAAAGGATTATTAGCTTTAAGAAAAGGGCTTGGTGTATTTGCGAACGTCCGCCCTGTCAC
GGTAGAAAGTGCAACGGCACATTTATCGCCATTGAAAAAGGCTGATGAAATTGATTTCGTTGTCGTTCGTGAATTAACAG
GTGGGATTTATTTTTCTTATCCGAAAGAACGAACGGACGAAGTAGCAACAGATACACTTACGTATCATCGCCATGAAATT
GAACGTATCGTTTCTTACGCCTTTCAATTAGCGAGTAAGCGGAAGAAAAAAGTAACATCTATTGATAAGGCGAATGTTTT
AGAATCTAGTAAACTATGGAGAACTGTAACAGAAGAAGTAGCACTTCGTTATCCAGATGTTGAACTAGAACATATTTTAG
TAGATGCGGCCGCTATGGAGCTAATTCGGAATCCAGGACGGTTTGATGTAATTGTAACAGAAAATTTATTTGGGGATATT
TTAAGTGACGAAGCTTCCGTATTAGCTGGATCATTAGGGATGCTTCCGTCAGCAAGTCACGCGGAAAAGGGACCTTCCTT
ATATGAGCCTATTCACGGATCGGCACCGGATATTGCCGGGAAAAATAAGGCGAATCCAATTGCTATGATGCGCTCTGTTG
CCATGATGCTTGGGCAATCGTTCGGATTAACGAGGGAAGGGTGTGCAATTGAAGAAGCGATTTCAGCAGTCCTTAAATCA
GGAAAATGTACAGCAGATATCGGGGGGACTGAAACGACAACTTCATTTACAAAGGCAGTTATGCAAGAAATGGAAGAACA
AGCGCTAGTAGGGAGAGGAAGATAA

Upstream 100 bases:

>100_bases
CTTTTATCCAGCTTGTGAAATAAGTGTTTGATAGGTGAGAGGAGCTATTCTCGCCTATCGAATTAAAGTTCTGAAAATTC
GGTTAAAAGGAGTGTTGAAA

Downstream 100 bases:

>100_bases
TGGGGAAAAGGTTGCTAGATAAGCTTTGGGAAAGACACGTAGTTACGACCAACGAAAATGGATTGGATTTATTATATATC
GATCTGCATCTTGTTCATGA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEHFGGVAIDLTGQPLPQRTLAACLASDAVLLGAVGGPRWD
GAKERPEKGLLALRKGLGVFANVRPVTVESATAHLSPLKKADEIDFVVVRELTGGIYFSYPKERTDEVATDTLTYHRHEI
ERIVSYAFQLASKRKKKVTSIDKANVLESSKLWRTVTEEVALRYPDVELEHILVDAAAMELIRNPGRFDVIVTENLFGDI
LSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLGQSFGLTREGCAIEEAISAVLKS
GKCTADIGGTETTTSFTKAVMQEMEEQALVGRGR

Sequences:

>Translated_354_residues
MEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEHFGGVAIDLTGQPLPQRTLAACLASDAVLLGAVGGPRWD
GAKERPEKGLLALRKGLGVFANVRPVTVESATAHLSPLKKADEIDFVVVRELTGGIYFSYPKERTDEVATDTLTYHRHEI
ERIVSYAFQLASKRKKKVTSIDKANVLESSKLWRTVTEEVALRYPDVELEHILVDAAAMELIRNPGRFDVIVTENLFGDI
LSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLGQSFGLTREGCAIEEAISAVLKS
GKCTADIGGTETTTSFTKAVMQEMEEQALVGRGR
>Mature_354_residues
MEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEHFGGVAIDLTGQPLPQRTLAACLASDAVLLGAVGGPRWD
GAKERPEKGLLALRKGLGVFANVRPVTVESATAHLSPLKKADEIDFVVVRELTGGIYFSYPKERTDEVATDTLTYHRHEI
ERIVSYAFQLASKRKKKVTSIDKANVLESSKLWRTVTEEVALRYPDVELEHILVDAAAMELIRNPGRFDVIVTENLFGDI
LSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLGQSFGLTREGCAIEEAISAVLKS
GKCTADIGGTETTTSFTKAVMQEMEEQALVGRGR

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=349, Percent_Identity=30.945558739255, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI28178821, Length=351, Percent_Identity=31.0541310541311, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI28178816, Length=345, Percent_Identity=31.304347826087, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI4758582, Length=255, Percent_Identity=31.3725490196078, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI28178819, Length=194, Percent_Identity=34.5360824742268, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI28178838, Length=230, Percent_Identity=31.304347826087, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI87081683, Length=333, Percent_Identity=51.0510510510511, Blast_Score=319, Evalue=1e-88,
Organism=Escherichia coli, GI1788101, Length=356, Percent_Identity=35.3932584269663, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1787381, Length=373, Percent_Identity=23.8605898123324, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71986051, Length=354, Percent_Identity=31.638418079096, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17550882, Length=352, Percent_Identity=30.3977272727273, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI25144293, Length=354, Percent_Identity=27.683615819209, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=347, Percent_Identity=27.3775216138329, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319830, Length=358, Percent_Identity=48.0446927374302, Blast_Score=290, Evalue=3e-79,
Organism=Saccharomyces cerevisiae, GI6322097, Length=357, Percent_Identity=36.6946778711485, Blast_Score=174, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6324709, Length=346, Percent_Identity=33.5260115606936, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6324291, Length=353, Percent_Identity=29.4617563739377, Blast_Score=131, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24643268, Length=348, Percent_Identity=32.183908045977, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24643270, Length=348, Percent_Identity=32.183908045977, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI281362242, Length=352, Percent_Identity=29.8295454545455, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24648872, Length=352, Percent_Identity=29.8295454545455, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24661184, Length=357, Percent_Identity=27.7310924369748, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI161078635, Length=348, Percent_Identity=27.0114942528736, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161078637, Length=348, Percent_Identity=27.0114942528736, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161078633, Length=348, Percent_Identity=27.0114942528736, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24650122, Length=348, Percent_Identity=27.0114942528736, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161078639, Length=345, Percent_Identity=27.2463768115942, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI20130355, Length=342, Percent_Identity=24.2690058479532, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38377; Mature: 38377

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEHFGGVAIDLTGQPLPQRTL
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCCCHHHH
AACLASDAVLLGAVGGPRWDGAKERPEKGLLALRKGLGVFANVRPVTVESATAHLSPLKK
HHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEECCCEEEECCHHHHHHHHHH
ADEIDFVVVRELTGGIYFSYPKERTDEVATDTLTYHRHEIERIVSYAFQLASKRKKKVTS
CCCCCEEEEEEHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDKANVLESSKLWRTVTEEVALRYPDVELEHILVDAAAMELIRNPGRFDVIVTENLFGDI
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHH
LSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLGQS
HCCHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
FGLTREGCAIEEAISAVLKSGKCTADIGGTETTTSFTKAVMQEMEEQALVGRGR
CCCCCCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEKRIVCLAGDGVGPEVMESAKEVLHMVERLYGHHFHLQDEHFGGVAIDLTGQPLPQRTL
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEEECCCCCCHHHH
AACLASDAVLLGAVGGPRWDGAKERPEKGLLALRKGLGVFANVRPVTVESATAHLSPLKK
HHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCEECCCEEEECCHHHHHHHHHH
ADEIDFVVVRELTGGIYFSYPKERTDEVATDTLTYHRHEIERIVSYAFQLASKRKKKVTS
CCCCCEEEEEEHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDKANVLESSKLWRTVTEEVALRYPDVELEHILVDAAAMELIRNPGRFDVIVTENLFGDI
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHH
LSDEASVLAGSLGMLPSASHAEKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLGQS
HCCHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
FGLTREGCAIEEAISAVLKSGKCTADIGGTETTTSFTKAVMQEMEEQALVGRGR
CCCCCCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]