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The map label for this gene is leuA [H]

Identifier: 218902608

GI number: 218902608

Start: 1409250

End: 1410770

Strand: Direct

Name: leuA [H]

Synonym: BCAH820_1491

Alternate gene names: 218902608

Gene position: 1409250-1410770 (Clockwise)

Preceding gene: 218902607

Following gene: 218902609

Centisome position: 26.58

GC content: 38.33

Gene sequence:

>1521_bases
ATGAAGCAAATTTTGTTCATGGATACGACGCTACGTGATGGCGAACAATCACCAGGAGTAAATTTAAATGAACAAGAGAA
ATTGCAAATTGCAAGGCAATTAGAGAGACTAGGGATTCATGTCATGGAAGCTGGCTTTGCAGCAGCTTCCGAAGGGGATT
TTCAATCGGTAAAGCGCATCGCAAATACAATTCAAAATGCTACAGTTATGAGTTTAGCAAGAGCGAAAGAAAGTGATATT
CGAAGAGCATATGAAGCTGTGAAAGGTGCGGTATCTCCTCGTTTACACGTATTTTTAGCTACGAGTGATATTCATATGAA
GTATAAACTTTGTATGTCAAAAGAAGATGTATTAGATAGTATTTATCGATCGGTTACACTTGGGAAATCGTTATTTCCGA
CAGTACAATTTTCAGCAGAAGATGCAACAAGAACAGCAAGAGATTTTTTAGCTGAAGCGGTAGAAGTTGCGATTCGTGCA
GGAGCGAATGTAATTAATATTCCAGATACAGTTGGGTATACGAATCCAGAAGAATATTATGCTCTTTTTAAATATTTACA
AGAATCTGTTCCTTCATATGAAAAAGCAATTTTCTCTTGTCATTGTCATGATGATCTTGGGATGGCGGTAGCAAATTCAT
TAGCTGCAGTTGAAGGCGGAGCGTTGCAAGTAGAAGGAACAATTAATGGGATTGGAGAAAGAGCAGGGAATGCGGCGTTA
GAAGAGGTTGCAGTTGCTCTTCATATTAGAAAAGATTTCTATAAAGCAGAGCCTTCTATGACGCTAAAAGAAATTAAAGC
GACAAGTACATTAGTAAGCAGATTAACGGGTATGGTTGTACCGAAAAATAAAGCGATTGTTGGAGCAAATGCATTCGCTC
ATGAATCAGGCATTCATCAAGATGGTGTATTAAAAGAAGTGACAACATATGAAATTATTGAACCAGCGCTAGTAGGCGAA
TCTCAAAATCTATTTGTACTTGGAAAACATTCTGGGCGTCACGCGTTTACAGAAAAAATGAAAGAACTTGGCTATGAATT
TACAAACGACGAGCGGGATGCAGTATTTGAAGCATTTAAAAAATTAGCTGATCGTAAGAAGGAAATTACTGAGGAAGATT
TACGAGCACTTATGCTTGGTGAAGCAGCATTTGCGGCTCAGCAATATAACATTACGCAATTGCAAGTACATTTCGTATCA
AATAGTACACAATGTGCGACAGTTGTACTAAAAGATGAGGAAGGAAATGTATTCGAAGATGCAGCTACTGGCTCTGGTAG
TATTGAAGCGATTTATAATGCAATCCAAAGAATTTTAGGATTGGAATGTGAATTGGCGGATTATCGCATACAATCTATTA
CACAAGGTCAAGATGCACTGGCTCATGTTCATGTTGAATTAAAAGAAGGAGCACATCAAGTATCAGGTTTTGGTGTTGCA
CAAGACGTATTGGAAGCGTCGGCAAGAGCATATGTCCACGCAGCGGGGAAATTGAAATCTTTTATCCAGCTTGTGAAATA
A

Upstream 100 bases:

>100_bases
AATGAGAAAGAAAATGAACATGAAATCGAAGTAGTTGGACGTAAATTACGTGAAATGATGCCTTTCGTACAGCCGCGAGT
AAAGGCAGGGATCAAATAAT

Downstream 100 bases:

>100_bases
GTGTTTGATAGGTGAGAGGAGCTATTCTCGCCTATCGAATTAAAGTTCTGAAAATTCGGTTAAAAGGAGTGTTGAAATTG
GAAAAACGTATCGTTTGTTT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MKQILFMDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRIANTIQNATVMSLARAKESDI
RRAYEAVKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLDSIYRSVTLGKSLFPTVQFSAEDATRTARDFLAEAVEVAIRA
GANVINIPDTVGYTNPEEYYALFKYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAAL
EEVAVALHIRKDFYKAEPSMTLKEIKATSTLVSRLTGMVVPKNKAIVGANAFAHESGIHQDGVLKEVTTYEIIEPALVGE
SQNLFVLGKHSGRHAFTEKMKELGYEFTNDERDAVFEAFKKLADRKKEITEEDLRALMLGEAAFAAQQYNITQLQVHFVS
NSTQCATVVLKDEEGNVFEDAATGSGSIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGAHQVSGFGVA
QDVLEASARAYVHAAGKLKSFIQLVK

Sequences:

>Translated_506_residues
MKQILFMDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRIANTIQNATVMSLARAKESDI
RRAYEAVKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLDSIYRSVTLGKSLFPTVQFSAEDATRTARDFLAEAVEVAIRA
GANVINIPDTVGYTNPEEYYALFKYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAAL
EEVAVALHIRKDFYKAEPSMTLKEIKATSTLVSRLTGMVVPKNKAIVGANAFAHESGIHQDGVLKEVTTYEIIEPALVGE
SQNLFVLGKHSGRHAFTEKMKELGYEFTNDERDAVFEAFKKLADRKKEITEEDLRALMLGEAAFAAQQYNITQLQVHFVS
NSTQCATVVLKDEEGNVFEDAATGSGSIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGAHQVSGFGVA
QDVLEASARAYVHAAGKLKSFIQLVK
>Mature_506_residues
MKQILFMDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRIANTIQNATVMSLARAKESDI
RRAYEAVKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLDSIYRSVTLGKSLFPTVQFSAEDATRTARDFLAEAVEVAIRA
GANVINIPDTVGYTNPEEYYALFKYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAAL
EEVAVALHIRKDFYKAEPSMTLKEIKATSTLVSRLTGMVVPKNKAIVGANAFAHESGIHQDGVLKEVTTYEIIEPALVGE
SQNLFVLGKHSGRHAFTEKMKELGYEFTNDERDAVFEAFKKLADRKKEITEEDLRALMLGEAAFAAQQYNITQLQVHFVS
NSTQCATVVLKDEEGNVFEDAATGSGSIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGAHQVSGFGVA
QDVLEASARAYVHAAGKLKSFIQLVK

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=493, Percent_Identity=45.4361054766734, Blast_Score=406, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6320071, Length=365, Percent_Identity=29.3150684931507, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320019, Length=361, Percent_Identity=29.3628808864266, Blast_Score=146, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6324225, Length=528, Percent_Identity=24.4318181818182, Blast_Score=137, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6324682, Length=529, Percent_Identity=24.1965973534972, Blast_Score=130, Evalue=4e-31,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 55385; Mature: 55385

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQILFMDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRI
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHHHH
ANTIQNATVMSLARAKESDIRRAYEAVKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEHHHHCCHHHHHHH
IYRSVTLGKSLFPTVQFSAEDATRTARDFLAEAVEVAIRAGANVINIPDTVGYTNPEEYY
HHHHHHCCCHHCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHH
ALFKYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAAL
HHHHHHHHHCCHHHHHHEEEECCCHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCHHHH
EEVAVALHIRKDFYKAEPSMTLKEIKATSTLVSRLTGMVVPKNKAIVGANAFAHESGIHQ
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCEEECCCCEEEECCHHHHCCCCCC
DGVLKEVTTYEIIEPALVGESQNLFVLGKHSGRHAFTEKMKELGYEFTNDERDAVFEAFK
CCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
KLADRKKEITEEDLRALMLGEAAFAAQQYNITQLQVHFVSNSTQCATVVLKDEEGNVFED
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEECCCCCEECC
AATGSGSIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGAHQVSGFGVA
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHEEEEEEHHHCHHHHCCCHHH
QDVLEASARAYVHAAGKLKSFIQLVK
HHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKQILFMDTTLRDGEQSPGVNLNEQEKLQIARQLERLGIHVMEAGFAAASEGDFQSVKRI
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCHHHHHHH
ANTIQNATVMSLARAKESDIRRAYEAVKGAVSPRLHVFLATSDIHMKYKLCMSKEDVLDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEHHHHCCHHHHHHH
IYRSVTLGKSLFPTVQFSAEDATRTARDFLAEAVEVAIRAGANVINIPDTVGYTNPEEYY
HHHHHHCCCHHCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHH
ALFKYLQESVPSYEKAIFSCHCHDDLGMAVANSLAAVEGGALQVEGTINGIGERAGNAAL
HHHHHHHHHCCHHHHHHEEEECCCHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCHHHH
EEVAVALHIRKDFYKAEPSMTLKEIKATSTLVSRLTGMVVPKNKAIVGANAFAHESGIHQ
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCEEECCCCEEEECCHHHHCCCCCC
DGVLKEVTTYEIIEPALVGESQNLFVLGKHSGRHAFTEKMKELGYEFTNDERDAVFEAFK
CCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
KLADRKKEITEEDLRALMLGEAAFAAQQYNITQLQVHFVSNSTQCATVVLKDEEGNVFED
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEECCCCCEECC
AATGSGSIEAIYNAIQRILGLECELADYRIQSITQGQDALAHVHVELKEGAHQVSGFGVA
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHEEEEEEHHHCHHHHCCCHHH
QDVLEASARAYVHAAGKLKSFIQLVK
HHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA