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The map label for this gene is tetB [H]

Identifier: 218902233

GI number: 218902233

Start: 1066068

End: 1067459

Strand: Direct

Name: tetB [H]

Synonym: BCAH820_1115

Alternate gene names: 218902233

Gene position: 1066068-1067459 (Clockwise)

Preceding gene: 218902232

Following gene: 218902234

Centisome position: 20.1

GC content: 39.08

Gene sequence:

>1392_bases
ATGGTAGATCATTCATCACAAAATGCAGATAAATTACTAGGAATTTTAGTAGTTACGCTAATATTTTCAGTTATGAACGG
TACGATGTTTAATGTAGCTCTTCCTGAAATCGGTAAAGAATTCAACCTTGTTCCTTCAGAGGTTAGTTGGATTATGACGA
GTTATATGGTCGTTTATGCTGTTGGTTCCATCGTAATGGGAAAACTGGCGGATAAATATCGGCTAAAAGATTTGTTAACG
TATGGATTACTTATTTTCGCTCTAGGTTCCTTGCTTGGCTTACTTGCTACTGAGTATTGGGTTATCATTTTAGGGCGTGT
TATTCAAGCAGCGGGAGCTTCTGTTCTTCCTGCGACAGCAATGATTATTCCGGTTAGATACTTTGCGCCAGAGAAAAGGG
GGCGTGCGCTTGGAACTTCAGCAGTTGGTTTAGCGCTTGGGAATGCTTTAGGGCCCGTTGCCGCTGGATTAATTACGAGT
TTTGGAAGCTGGAGACTCATGTTTGTTCTTTCATTACTACCGCTTCTAACGCTACCTTTCTTTAGAAAGTATTTAGACAA
TGCAAAAGGGAAGGCGGGTAGTATTGATATTCTTGGCGGAGGTCTATTAGCTGTATCAGTCGCGTTCTTCTTGCTTGCTA
TTACGCAGATGCAAGTGCTGTTATTCCTTAGTGGATTTGCTACGTTAGCTTTCTTCATTTTGCGGATTAGAAAAGCTAAA
GAGCCTTTTATTAAACCGATTCTGTTTAAAAATAAGAACTTTTCGATTGGGTTACTCCTTGCTTTTATGACGACAGCGAT
GAGCTTTAGTATGACATTCATGACGCCGCAATTTCTATCGGCAGTAAATGGATTGACTCCTTCGAATATTGGTTTTGTTT
TAGTTCCAGCTGCAATTGCCTCGGCAATTATGGGAAGAAAGGGAGGAAGGGTAGCTGATACTCGGGGCAATTTTGCACTC
GTTTTTATTGCCTCAGTATTCATATTCCTTGCTTTCTCATTATTATCTACTTTCATTGGGATCTCGGCTTTTGTCATAGC
ATTGATTTTAATTTTCGGAAATGTAGGACAGACCTTGATGCAGATTTCAATGTCTAATACAATTTCACAAACACTGTCAA
AGGAAGAGACAGGTGTTGGAATGGGATTACTGTCGATGATTAATTTCATATCTGGCGCGATGGCTATGAGTGTAGTAGGG
AAATTGCTTGATAAGGGATCGACTTCACTAAAGCTAAATCCTTTTGTTGCAAATGAAGCTGCAAATATGTATAGTAATAT
CTTCGGCGTGATGTCATTATTGATCCTACTTGTAGTTATGCTGTATCGTTTTCAATTTGGTACGGGCGTTTCTACGTTAA
ATATGACTAGCAAAGTTAATAAAAATTTATAA

Upstream 100 bases:

>100_bases
AATGATATCCTCATTATAATAAAATAGTGAGGATATTCATTATGTTGATACTCATTTAAATCTGAATAGATTACCGGAAA
AAGAAAAGGAGGTATGCACG

Downstream 100 bases:

>100_bases
AAGAATACAAGGAGGTTTATGTATGAATGCTCAAACTGCACCTGTTTCTTCGCAGTCAAAGGAGTTTGACAATGTGAAAA
GACTCCCCATTTTAATATCG

Product: major facilitator family transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MVDHSSQNADKLLGILVVTLIFSVMNGTMFNVALPEIGKEFNLVPSEVSWIMTSYMVVYAVGSIVMGKLADKYRLKDLLT
YGLLIFALGSLLGLLATEYWVIILGRVIQAAGASVLPATAMIIPVRYFAPEKRGRALGTSAVGLALGNALGPVAAGLITS
FGSWRLMFVLSLLPLLTLPFFRKYLDNAKGKAGSIDILGGGLLAVSVAFFLLAITQMQVLLFLSGFATLAFFILRIRKAK
EPFIKPILFKNKNFSIGLLLAFMTTAMSFSMTFMTPQFLSAVNGLTPSNIGFVLVPAAIASAIMGRKGGRVADTRGNFAL
VFIASVFIFLAFSLLSTFIGISAFVIALILIFGNVGQTLMQISMSNTISQTLSKEETGVGMGLLSMINFISGAMAMSVVG
KLLDKGSTSLKLNPFVANEAANMYSNIFGVMSLLILLVVMLYRFQFGTGVSTLNMTSKVNKNL

Sequences:

>Translated_463_residues
MVDHSSQNADKLLGILVVTLIFSVMNGTMFNVALPEIGKEFNLVPSEVSWIMTSYMVVYAVGSIVMGKLADKYRLKDLLT
YGLLIFALGSLLGLLATEYWVIILGRVIQAAGASVLPATAMIIPVRYFAPEKRGRALGTSAVGLALGNALGPVAAGLITS
FGSWRLMFVLSLLPLLTLPFFRKYLDNAKGKAGSIDILGGGLLAVSVAFFLLAITQMQVLLFLSGFATLAFFILRIRKAK
EPFIKPILFKNKNFSIGLLLAFMTTAMSFSMTFMTPQFLSAVNGLTPSNIGFVLVPAAIASAIMGRKGGRVADTRGNFAL
VFIASVFIFLAFSLLSTFIGISAFVIALILIFGNVGQTLMQISMSNTISQTLSKEETGVGMGLLSMINFISGAMAMSVVG
KLLDKGSTSLKLNPFVANEAANMYSNIFGVMSLLILLVVMLYRFQFGTGVSTLNMTSKVNKNL
>Mature_463_residues
MVDHSSQNADKLLGILVVTLIFSVMNGTMFNVALPEIGKEFNLVPSEVSWIMTSYMVVYAVGSIVMGKLADKYRLKDLLT
YGLLIFALGSLLGLLATEYWVIILGRVIQAAGASVLPATAMIIPVRYFAPEKRGRALGTSAVGLALGNALGPVAAGLITS
FGSWRLMFVLSLLPLLTLPFFRKYLDNAKGKAGSIDILGGGLLAVSVAFFLLAITQMQVLLFLSGFATLAFFILRIRKAK
EPFIKPILFKNKNFSIGLLLAFMTTAMSFSMTFMTPQFLSAVNGLTPSNIGFVLVPAAIASAIMGRKGGRVADTRGNFAL
VFIASVFIFLAFSLLSTFIGISAFVIALILIFGNVGQTLMQISMSNTISQTLSKEETGVGMGLLSMINFISGAMAMSVVG
KLLDKGSTSLKLNPFVANEAANMYSNIFGVMSLLILLVVMLYRFQFGTGVSTLNMTSKVNKNL

Specific function: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=430, Percent_Identity=24.1860465116279, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081983, Length=410, Percent_Identity=23.9024390243902, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1789042, Length=334, Percent_Identity=23.9520958083832, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788392, Length=405, Percent_Identity=23.9506172839506, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788710, Length=317, Percent_Identity=24.9211356466877, Blast_Score=62, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6321663, Length=139, Percent_Identity=27.3381294964029, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49654; Mature: 49654

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDHSSQNADKLLGILVVTLIFSVMNGTMFNVALPEIGKEFNLVPSEVSWIMTSYMVVYA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECHHHCCCCCCCHHHHHHHHHHHHHHHH
VGSIVMGKLADKYRLKDLLTYGLLIFALGSLLGLLATEYWVIILGRVIQAAGASVLPATA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
MIIPVRYFAPEKRGRALGTSAVGLALGNALGPVAAGLITSFGSWRLMFVLSLLPLLTLPF
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
FRKYLDNAKGKAGSIDILGGGLLAVSVAFFLLAITQMQVLLFLSGFATLAFFILRIRKAK
HHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EPFIKPILFKNKNFSIGLLLAFMTTAMSFSMTFMTPQFLSAVNGLTPSNIGFVLVPAAIA
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCEEHHHHHH
SAIMGRKGGRVADTRGNFALVFIASVFIFLAFSLLSTFIGISAFVIALILIFGNVGQTLM
HHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QISMSNTISQTLSKEETGVGMGLLSMINFISGAMAMSVVGKLLDKGSTSLKLNPFVANEA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHH
ANMYSNIFGVMSLLILLVVMLYRFQFGTGVSTLNMTSKVNKNL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MVDHSSQNADKLLGILVVTLIFSVMNGTMFNVALPEIGKEFNLVPSEVSWIMTSYMVVYA
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECHHHCCCCCCCHHHHHHHHHHHHHHHH
VGSIVMGKLADKYRLKDLLTYGLLIFALGSLLGLLATEYWVIILGRVIQAAGASVLPATA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
MIIPVRYFAPEKRGRALGTSAVGLALGNALGPVAAGLITSFGSWRLMFVLSLLPLLTLPF
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
FRKYLDNAKGKAGSIDILGGGLLAVSVAFFLLAITQMQVLLFLSGFATLAFFILRIRKAK
HHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EPFIKPILFKNKNFSIGLLLAFMTTAMSFSMTFMTPQFLSAVNGLTPSNIGFVLVPAAIA
CCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCEEHHHHHH
SAIMGRKGGRVADTRGNFALVFIASVFIFLAFSLLSTFIGISAFVIALILIFGNVGQTLM
HHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QISMSNTISQTLSKEETGVGMGLLSMINFISGAMAMSVVGKLLDKGSTSLKLNPFVANEA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHH
ANMYSNIFGVMSLLILLVVMLYRFQFGTGVSTLNMTSKVNKNL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2844262; 7584024; 9384377 [H]