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The map label for this gene is glpF [H]

Identifier: 218902223

GI number: 218902223

Start: 1056877

End: 1057698

Strand: Direct

Name: glpF [H]

Synonym: BCAH820_1105

Alternate gene names: 218902223

Gene position: 1056877-1057698 (Clockwise)

Preceding gene: 218902222

Following gene: 218902224

Centisome position: 19.93

GC content: 40.51

Gene sequence:

>822_bases
ATGTCAGCATTTTTAGGAGAGTTAATAGGGACTGCGTTGTTAATCGTACTTGGTGGCGGCGTTTGTGCTGGGGTAAGTTT
AAAGAAGTCGTTTGCGAAAGATTCTGGTTGGATTGTTATTACAATGGGCTGGGGCTTAGCGGTAGCGGTTGCAGCGTACG
CGGTTGGATCAATTAGTGGGGCACATTTGAATCCAGCTTTGACGATAGGATTAGCATTTAAGGGAGCGTTCCCATGGGGT
GACGTACCTGGTTATATCGCAGCACAAATGATTGGGGCAATTATCGGGGCAGTTATCGTATATTTACATTACTTACCACA
CTGGAAAGAAACAGAAGATCCAGGAACAAAGTTAGGTGTATTTGCAACAGGTCCAGCAATTCCGAACACATTTGCAAACC
TTTTAAGTGAAATGATTGGGACATTCGTTTTAGTATTTGGTATATTAGCAATTGGTGCAAATAAATTTGCAGATGGATTA
AATCCATTTATCGTAGGTTTCTTAATTGTAAGTATTGGTTTATCATTAGGTGGAACGACAGGATATGCAATCAACCCAGC
ACGTGATTTAGGTCCGCGTATCGCGCACTTCCTCCTTCCGATTGCAGGAAAAGGCGGTTCAAACTGGAAGTATGCATGGA
TTCCAGTAGTAGGTCCGATTTTAGGTGGATCACTTGCAGGATTATTCCATCAAGTTGTATTTGAAGGAAAACAAAATGCA
GCACTTATTTATGTAATTATTGCAACTGTGATTGTACTAGCAATCTCTTATATGACAAGCAAAAAAAGTGGAAGCAATAC
AAATAGTAGAAAAGTAGCATAA

Upstream 100 bases:

>100_bases
GTGCAAAGCTGTGGGTCTTTTTCTTTTGACAAAAACGAATGGCCATTCATTTTACCTCTATCACATTTGAAAGCGTTTAA
ATTGATTGTGGAGGGATACT

Downstream 100 bases:

>100_bases
GGGGGAAACCATTATGAAAAAATATATTCTTTCATTAGACCAAGGAACAACTAGCTCACGCGCAATTCTTTTCAATAAAA
AAGGTGAAATTGTTCATTCA

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYAVGSISGAHLNPALTIGLAFKGAFPWG
DVPGYIAAQMIGAIIGAVIVYLHYLPHWKETEDPGTKLGVFATGPAIPNTFANLLSEMIGTFVLVFGILAIGANKFADGL
NPFIVGFLIVSIGLSLGGTTGYAINPARDLGPRIAHFLLPIAGKGGSNWKYAWIPVVGPILGGSLAGLFHQVVFEGKQNA
ALIYVIIATVIVLAISYMTSKKSGSNTNSRKVA

Sequences:

>Translated_273_residues
MSAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYAVGSISGAHLNPALTIGLAFKGAFPWG
DVPGYIAAQMIGAIIGAVIVYLHYLPHWKETEDPGTKLGVFATGPAIPNTFANLLSEMIGTFVLVFGILAIGANKFADGL
NPFIVGFLIVSIGLSLGGTTGYAINPARDLGPRIAHFLLPIAGKGGSNWKYAWIPVVGPILGGSLAGLFHQVVFEGKQNA
ALIYVIIATVIVLAISYMTSKKSGSNTNSRKVA
>Mature_272_residues
SAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYAVGSISGAHLNPALTIGLAFKGAFPWGD
VPGYIAAQMIGAIIGAVIVYLHYLPHWKETEDPGTKLGVFATGPAIPNTFANLLSEMIGTFVLVFGILAIGANKFADGLN
PFIVGFLIVSIGLSLGGTTGYAINPARDLGPRIAHFLLPIAGKGGSNWKYAWIPVVGPILGGSLAGLFHQVVFEGKQNAA
LIYVIIATVIVLAISYMTSKKSGSNTNSRKVA

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=242, Percent_Identity=37.603305785124, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI157266307, Length=248, Percent_Identity=35.8870967741936, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI4502187, Length=249, Percent_Identity=34.9397590361446, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI22538420, Length=243, Percent_Identity=32.0987654320988, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI4755125, Length=206, Percent_Identity=34.9514563106796, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI4502181, Length=206, Percent_Identity=34.9514563106796, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI37694062, Length=245, Percent_Identity=31.0204081632653, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI310133356, Length=200, Percent_Identity=30.5, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI310114181, Length=200, Percent_Identity=30.5, Blast_Score=72, Evalue=4e-13,
Organism=Escherichia coli, GI1790362, Length=235, Percent_Identity=36.5957446808511, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787101, Length=189, Percent_Identity=33.8624338624339, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI71994009, Length=229, Percent_Identity=33.1877729257642, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17544068, Length=246, Percent_Identity=34.9593495934959, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17533613, Length=240, Percent_Identity=30.4166666666667, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI32564052, Length=240, Percent_Identity=30.4166666666667, Blast_Score=97, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI71992966, Length=234, Percent_Identity=31.1965811965812, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17531431, Length=259, Percent_Identity=30.1158301158301, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17531429, Length=259, Percent_Identity=30.1158301158301, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17558372, Length=236, Percent_Identity=28.8135593220339, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71992961, Length=224, Percent_Identity=29.9107142857143, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71993722, Length=195, Percent_Identity=29.2307692307692, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321054, Length=206, Percent_Identity=35.9223300970874, Blast_Score=113, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6322985, Length=290, Percent_Identity=27.2413793103448, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24652747, Length=239, Percent_Identity=32.6359832635983, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI45551084, Length=239, Percent_Identity=32.6359832635983, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24762342, Length=252, Percent_Identity=28.1746031746032, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24762344, Length=243, Percent_Identity=32.0987654320988, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24762346, Length=243, Percent_Identity=32.0987654320988, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24762348, Length=243, Percent_Identity=32.0987654320988, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20130305, Length=243, Percent_Identity=32.0987654320988, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922828, Length=229, Percent_Identity=28.3842794759825, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 28170; Mature: 28038

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYAVGSISG
CCHHHHHHHHHHHHHHHCCCHHHCHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCC
AHLNPALTIGLAFKGAFPWGDVPGYIAAQMIGAIIGAVIVYLHYLPHWKETEDPGTKLGV
CCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
FATGPAIPNTFANLLSEMIGTFVLVFGILAIGANKFADGLNPFIVGFLIVSIGLSLGGTT
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHCCCCC
GYAINPARDLGPRIAHFLLPIAGKGGSNWKYAWIPVVGPILGGSLAGLFHQVVFEGKQNA
CCEECCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCC
ALIYVIIATVIVLAISYMTSKKSGSNTNSRKVA
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAFLGELIGTALLIVLGGGVCAGVSLKKSFAKDSGWIVITMGWGLAVAVAAYAVGSISG
CHHHHHHHHHHHHHHHCCCHHHCHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCC
AHLNPALTIGLAFKGAFPWGDVPGYIAAQMIGAIIGAVIVYLHYLPHWKETEDPGTKLGV
CCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
FATGPAIPNTFANLLSEMIGTFVLVFGILAIGANKFADGLNPFIVGFLIVSIGLSLGGTT
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHCCCCC
GYAINPARDLGPRIAHFLLPIAGKGGSNWKYAWIPVVGPILGGSLAGLFHQVVFEGKQNA
CCEECCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCC
ALIYVIIATVIVLAISYMTSKKSGSNTNSRKVA
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8436953; 9579061; 9384377; 2127799 [H]