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The map label for this gene is tenI [H]

Identifier: 218901924

GI number: 218901924

Start: 769723

End: 770343

Strand: Direct

Name: tenI [H]

Synonym: BCAH820_0806

Alternate gene names: 218901924

Gene position: 769723-770343 (Clockwise)

Preceding gene: 218901923

Following gene: 218901925

Centisome position: 14.52

GC content: 38.81

Gene sequence:

>621_bases
ATGAAAAATGAGCTTCATGTAATCTCAAATGGTCACATGTCATTCGAAGAGTTAGTGAGTGTAGCGATGCAAATTGAGAG
TGAGATTGATTATTTGCATATTCGTGAGCGTGAAAAAAGTACGAAGGAATTGTATGAAGGGGTGGAAAGCCTTTTGAAAA
AAGGCTTTCCAGCATCAAAGCTTGTTATCAATGATCGAATTGATATTGCTATTTTATTAAATATCCCGCGTGTACAGCTA
GGGTACCGAAGCACAGATGTAAGGTCAGTGAAAGAAAAGTTTTCTTATTTGCATGTCGGTTATTCCGTACATTCTTTAGA
AGAGGCGATAGAGGCTTTTAAGAACGGGGCTGATTCGCTCGTTTATGGTCATGTATTCCCGACAGAGTGTAAAAAAGGTG
TGCCAGCGAGAGGGTTAGAGGAAATTTCGGACATTGCAAGGAGCTTATCGATACCAATTATAGCTATTGGAGGGATTACT
CCTGAAAACACAAAGGATATTTTGGCAAGTGAAGTAAGTGGAATTGCTGTTATGTCTGGAATTGTAAGTAGTAGTAACCC
ATATAGCAAAGCGAAGTCTTATAAGGAATCAATAAGAAAGTGGGCGGAAAAACATGTGTAA

Upstream 100 bases:

>100_bases
TGGGAGAAACAAAATAAATGGTTGAAAGACAAAGGAATGACGAAAGAAATCGTTCCAGCCAATGAGCTATTCGAAAACAT
TTTAAAGTAGGCGAATGAAT

Downstream 100 bases:

>100_bases
GAAGTATGATGTAGCGATTATTGGCGGAGGTGTAATTGGTAGTTCAGTTGCACATTTTCTAGCAGAAAGAGGACATAAAG
TAGCGATTGTAGAGAAGCAA

Product: transcriptional regulator TenI

Products: Diphosphate; Thiamin monophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MKNELHVISNGHMSFEELVSVAMQIESEIDYLHIREREKSTKELYEGVESLLKKGFPASKLVINDRIDIAILLNIPRVQL
GYRSTDVRSVKEKFSYLHVGYSVHSLEEAIEAFKNGADSLVYGHVFPTECKKGVPARGLEEISDIARSLSIPIIAIGGIT
PENTKDILASEVSGIAVMSGIVSSSNPYSKAKSYKESIRKWAEKHV

Sequences:

>Translated_206_residues
MKNELHVISNGHMSFEELVSVAMQIESEIDYLHIREREKSTKELYEGVESLLKKGFPASKLVINDRIDIAILLNIPRVQL
GYRSTDVRSVKEKFSYLHVGYSVHSLEEAIEAFKNGADSLVYGHVFPTECKKGVPARGLEEISDIARSLSIPIIAIGGIT
PENTKDILASEVSGIAVMSGIVSSSNPYSKAKSYKESIRKWAEKHV
>Mature_206_residues
MKNELHVISNGHMSFEELVSVAMQIESEIDYLHIREREKSTKELYEGVESLLKKGFPASKLVINDRIDIAILLNIPRVQL
GYRSTDVRSVKEKFSYLHVGYSVHSLEEAIEAFKNGADSLVYGHVFPTECKKGVPARGLEEISDIARSLSIPIIAIGGIT
PENTKDILASEVSGIAVMSGIVSSSNPYSKAKSYKESIRKWAEKHV

Specific function: Reduces the stimulatory effect of the tenA transcriptional activator on production of certain extracellular enzymes. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: 2.5.1.3 [C]

Molecular weight: Translated: 22891; Mature: 22891

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNELHVISNGHMSFEELVSVAMQIESEIDYLHIREREKSTKELYEGVESLLKKGFPASK
CCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LVINDRIDIAILLNIPRVQLGYRSTDVRSVKEKFSYLHVGYSVHSLEEAIEAFKNGADSL
EEEECCCCEEEEEECCCEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCE
VYGHVFPTECKKGVPARGLEEISDIARSLSIPIIAIGGITPENTKDILASEVSGIAVMSG
EECCCCHHHHHCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHH
IVSSSNPYSKAKSYKESIRKWAEKHV
HHCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNELHVISNGHMSFEELVSVAMQIESEIDYLHIREREKSTKELYEGVESLLKKGFPASK
CCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LVINDRIDIAILLNIPRVQLGYRSTDVRSVKEKFSYLHVGYSVHSLEEAIEAFKNGADSL
EEEECCCCEEEEEECCCEEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCE
VYGHVFPTECKKGVPARGLEEISDIARSLSIPIIAIGGITPENTKDILASEVSGIAVMSG
EECCCCHHHHHCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHH
IVSSSNPYSKAKSYKESIRKWAEKHV
HHCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0004 {thiazole} 0.00085 {hydroxymethylpyrimidine} [C]

Substrates: 2-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate; 4-Methyl-5-(2-phosphoethyl)-thiazole; H+ [C]

Specific reaction: 2-Methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-Methyl-5-(2-phosphoethyl)-thiazole + H+ --> Diphosphate + Thiamin monophosphate [C]

General reaction: Hydroxymethylpyrimidine group transfer [C]

Inhibitor: Acetyl phosphate substrates; ADP; ATP; CTP; Diphosphate; GTP; Phosphocreatine; Phosphoenolpyruvate; UTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]