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The map label for this gene is thiO [H]

Identifier: 218901925

GI number: 218901925

Start: 770336

End: 771445

Strand: Direct

Name: thiO [H]

Synonym: BCAH820_0807

Alternate gene names: 218901925

Gene position: 770336-771445 (Clockwise)

Preceding gene: 218901924

Following gene: 218901926

Centisome position: 14.53

GC content: 39.64

Gene sequence:

>1110_bases
ATGTGTAAGAAGTATGATGTAGCGATTATTGGCGGAGGTGTAATTGGTAGTTCAGTTGCACATTTTCTAGCAGAAAGAGG
ACATAAAGTAGCGATTGTAGAGAAGCAACAGATTGCATCTGAAGCCTCGAAAGCAGCTGCTGGTCTACTTGGTGTTCAGG
CAGAATGGGATGCATATGATCCACTATTTGATCTTGCTAGAGAAAGCCGTGCTATATTTCCACAACTTGCAGCAGTTTTA
CGTGAAAAAACAGGTATCGATATTGGGTATGAAGAGAAAGGTATTTACCGCATTGCTCAAAATGAAGCGGAGAGAGAAAG
AATTCTTAATATTATGGATTGGCAGCAGAAAACAGGTGAAGATTCTTACTTTCTAACGGGAGATCGTTTACGAGAGCAAG
AGCCGTATCTATCTGAGTCAATTATAGGTGCTGTATATTATCCAAAAGATGGCCATGTTATTGCGCCAGAGCTTACGAAA
GCATTTGCGCATTCTGCGGCAATTTCCGGTGCAGATATATATGAGCAAACAGAAGTGTTTGATATTCGTATTGAAAATAA
GAAAGTGATTGGGATTGTTACAAGTGAAGGTATGATCTCGTGCGAGAAAGTTGTTATTGCCGGTGGTTCATGGAGCACGA
AGTTACTAGGTTATTTTCACCGCGAATGGGGTACATATCCAGTTAAAGGAGAAGTAGTAGCAGTAAAAAGTAGAAAACAA
CTTTTAAAAGCACCTATTTTCCAAGAAAGATTTTACATTGCCCCAAAACGCGGCGGACGTTACGTAATTGGAGCAACGAT
GAAGCCACATACGTTCAATAAAACTGTGCACCCAGAAAGTATTACTTCTATATTAGAGCGTGCTTATACAATATTACCAG
CTTTAAAAGAAGCAGAATGGGAAAGTACGTGGGCAGGGCTAAGACCACAATCGAATCATGAAGCTCCTTATATGGGAGAG
CATGAAGAAATAAAAGGTTTATATGCTTGCACCGGCCATTATCGAAACGGTATTTTATTAAGTCCTGTTTCTGGTCAATA
TATGGCTGATTTAATAGAAGGAAAGCAAGAGAATCACTTGCTAGATTCATTGCTTTCTAAAACGGTTTAG

Upstream 100 bases:

>100_bases
GTAAGTGGAATTGCTGTTATGTCTGGAATTGTAAGTAGTAGTAACCCATATAGCAAAGCGAAGTCTTATAAGGAATCAAT
AAGAAAGTGGGCGGAAAAAC

Downstream 100 bases:

>100_bases
AAAGGGGATGGAAGTTTGAATTTGAAAATTAATGGTAATCAAATTGAAGTGCCAGAGAGTGTAAAAACAGTAGCCGAGCT
ACTTACACATTTAGAGTTAG

Product: glycine oxidase ThiO

Products: NA

Alternate protein names: GO [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MCKKYDVAIIGGGVIGSSVAHFLAERGHKVAIVEKQQIASEASKAAAGLLGVQAEWDAYDPLFDLARESRAIFPQLAAVL
REKTGIDIGYEEKGIYRIAQNEAERERILNIMDWQQKTGEDSYFLTGDRLREQEPYLSESIIGAVYYPKDGHVIAPELTK
AFAHSAAISGADIYEQTEVFDIRIENKKVIGIVTSEGMISCEKVVIAGGSWSTKLLGYFHREWGTYPVKGEVVAVKSRKQ
LLKAPIFQERFYIAPKRGGRYVIGATMKPHTFNKTVHPESITSILERAYTILPALKEAEWESTWAGLRPQSNHEAPYMGE
HEEIKGLYACTGHYRNGILLSPVSGQYMADLIEGKQENHLLDSLLSKTV

Sequences:

>Translated_369_residues
MCKKYDVAIIGGGVIGSSVAHFLAERGHKVAIVEKQQIASEASKAAAGLLGVQAEWDAYDPLFDLARESRAIFPQLAAVL
REKTGIDIGYEEKGIYRIAQNEAERERILNIMDWQQKTGEDSYFLTGDRLREQEPYLSESIIGAVYYPKDGHVIAPELTK
AFAHSAAISGADIYEQTEVFDIRIENKKVIGIVTSEGMISCEKVVIAGGSWSTKLLGYFHREWGTYPVKGEVVAVKSRKQ
LLKAPIFQERFYIAPKRGGRYVIGATMKPHTFNKTVHPESITSILERAYTILPALKEAEWESTWAGLRPQSNHEAPYMGE
HEEIKGLYACTGHYRNGILLSPVSGQYMADLIEGKQENHLLDSLLSKTV
>Mature_369_residues
MCKKYDVAIIGGGVIGSSVAHFLAERGHKVAIVEKQQIASEASKAAAGLLGVQAEWDAYDPLFDLARESRAIFPQLAAVL
REKTGIDIGYEEKGIYRIAQNEAERERILNIMDWQQKTGEDSYFLTGDRLREQEPYLSESIIGAVYYPKDGHVIAPELTK
AFAHSAAISGADIYEQTEVFDIRIENKKVIGIVTSEGMISCEKVVIAGGSWSTKLLGYFHREWGTYPVKGEVVAVKSRKQ
LLKAPIFQERFYIAPKRGGRYVIGATMKPHTFNKTVHPESITSILERAYTILPALKEAEWESTWAGLRPQSNHEAPYMGE
HEEIKGLYACTGHYRNGILLSPVSGQYMADLIEGKQENHLLDSLLSKTV

Specific function: Catalyzes the FAD-dependent oxidative deamination of various amines and D-amino acids to yield the corresponding alpha- keto acids, ammonia/amine, and hydrogen peroxide. Oxidizes sarcosine (N-methylglycine), N-ethylglycine and glycine. Can also oxidize th

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DAO family [H]

Homologues:

Organism=Homo sapiens, GI24797151, Length=380, Percent_Identity=24.2105263157895, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI194306651, Length=211, Percent_Identity=26.5402843601896, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI197927446, Length=383, Percent_Identity=22.7154046997389, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI21361378, Length=383, Percent_Identity=22.7154046997389, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1787438, Length=404, Percent_Identity=24.5049504950495, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI32563613, Length=389, Percent_Identity=24.6786632390745, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71994045, Length=390, Percent_Identity=23.5897435897436, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71994052, Length=397, Percent_Identity=23.1738035264484, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20130091, Length=364, Percent_Identity=25.8241758241758, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR012727 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.4.3.19 [H]

Molecular weight: Translated: 41108; Mature: 41108

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCKKYDVAIIGGGVIGSSVAHFLAERGHKVAIVEKQQIASEASKAAAGLLGVQAEWDAYD
CCCEEEEEEEECCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHEEECCCCCCCC
PLFDLARESRAIFPQLAAVLREKTGIDIGYEEKGIYRIAQNEAERERILNIMDWQQKTGE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHCHHHHHHHHHHHHHHHHCCC
DSYFLTGDRLREQEPYLSESIIGAVYYPKDGHVIAPELTKAFAHSAAISGADIYEQTEVF
CCEEECCHHHHCCCCCHHHHHEEEEEECCCCCEECHHHHHHHHHHHHCCCCHHHCCCEEE
DIRIENKKVIGIVTSEGMISCEKVVIAGGSWSTKLLGYFHREWGTYPVKGEVVAVKSRKQ
EEEECCCEEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHH
LLKAPIFQERFYIAPKRGGRYVIGATMKPHTFNKTVHPESITSILERAYTILPALKEAEW
HHHCCCHHHHEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
ESTWAGLRPQSNHEAPYMGEHEEIKGLYACTGHYRNGILLSPVSGQYMADLIEGKQENHL
HHHHCCCCCCCCCCCCCCCCHHHHCEEEEEECCCCCCEEECCCCCHHHHHHHCCCCHHHH
LDSLLSKTV
HHHHHHHCC
>Mature Secondary Structure
MCKKYDVAIIGGGVIGSSVAHFLAERGHKVAIVEKQQIASEASKAAAGLLGVQAEWDAYD
CCCEEEEEEEECCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHEEECCCCCCCC
PLFDLARESRAIFPQLAAVLREKTGIDIGYEEKGIYRIAQNEAERERILNIMDWQQKTGE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHCHHHHHHHHHHHHHHHHCCC
DSYFLTGDRLREQEPYLSESIIGAVYYPKDGHVIAPELTKAFAHSAAISGADIYEQTEVF
CCEEECCHHHHCCCCCHHHHHEEEEEECCCCCEECHHHHHHHHHHHHCCCCHHHCCCEEE
DIRIENKKVIGIVTSEGMISCEKVVIAGGSWSTKLLGYFHREWGTYPVKGEVVAVKSRKQ
EEEECCCEEEEEEECCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHH
LLKAPIFQERFYIAPKRGGRYVIGATMKPHTFNKTVHPESITSILERAYTILPALKEAEW
HHHCCCHHHHEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
ESTWAGLRPQSNHEAPYMGEHEEIKGLYACTGHYRNGILLSPVSGQYMADLIEGKQENHL
HHHHCCCCCCCCCCCCCCCCHHHHCEEEEEECCCCCCEEECCCCCHHHHHHHCCCCHHHH
LDSLLSKTV
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 9827558; 11744710 [H]