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The map label for this gene is ywkD [H]

Identifier: 218901802

GI number: 218901802

Start: 635143

End: 635451

Strand: Direct

Name: ywkD [H]

Synonym: BCAH820_0663

Alternate gene names: 218901802

Gene position: 635143-635451 (Clockwise)

Preceding gene: 218901798

Following gene: 218901803

Centisome position: 11.98

GC content: 37.54

Gene sequence:

>309_bases
TTGGGTTTTAAAGCGATAAATGAAGTATATAGAAAGGAAAGAGATTCTTATAAGTTAGATTTATGTGTAGGAAAAGAGTA
TCAAATTGAATTATTTTCATTTCCGAATCCTCCGAAGCGTCCGAGCTTCCCAGAAGCGGCTGGACTTAGGCATTTAGCAT
TTGCTGTTACAAATATAGAAGAAGCCGTGCAACATTTAAGTCAATGTGGCGTTGAAACTGAGGCGATACGTATTGATGAC
ATAACCGGGAAAAAATTTGTTTTTTTCCAAGACCCTGACGGTCTACCGTTAGAATTGTATGAGGTTTGA

Upstream 100 bases:

>100_bases
TAAAGAAAGGAATAAAGAAATCATGAATATAAGTAGAGTGCACCACGTTGCAATTATTTGTTCGAATTATGAAGTGTCAA
AGGATTTTTATACTAGGATA

Downstream 100 bases:

>100_bases
AATTGGTGAATTTTATATAGGGTTGCTTTTGAGTAAATGAAGAAACTAAAGTGAAACTTCTTTTTAAAAGGAGGTGTAAC
TTTAGCTAAGAAAGCTAAAG

Product: glyoxylase family protein

Products: glutathione; methylglyoxal

Alternate protein names: NA

Number of amino acids: Translated: 102; Mature: 101

Protein sequence:

>102_residues
MGFKAINEVYRKERDSYKLDLCVGKEYQIELFSFPNPPKRPSFPEAAGLRHLAFAVTNIEEAVQHLSQCGVETEAIRIDD
ITGKKFVFFQDPDGLPLELYEV

Sequences:

>Translated_102_residues
MGFKAINEVYRKERDSYKLDLCVGKEYQIELFSFPNPPKRPSFPEAAGLRHLAFAVTNIEEAVQHLSQCGVETEAIRIDD
ITGKKFVFFQDPDGLPLELYEV
>Mature_101_residues
GFKAINEVYRKERDSYKLDLCVGKEYQIELFSFPNPPKRPSFPEAAGLRHLAFAVTNIEEAVQHLSQCGVETEAIRIDDI
TGKKFVFFQDPDGLPLELYEV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081697, Length=101, Percent_Identity=61.3861386138614, Blast_Score=129, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: 4.4.1.5

Molecular weight: Translated: 11666; Mature: 11535

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFKAINEVYRKERDSYKLDLCVGKEYQIELFSFPNPPKRPSFPEAAGLRHLAFAVTNIE
CCHHHHHHHHHCCCCCEEEEEEECCCEEEEEEECCCCCCCCCCCCHHCHHHHHHHHHHHH
EAVQHLSQCGVETEAIRIDDITGKKFVFFQDPDGLPLELYEV
HHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCEEEEEC
>Mature Secondary Structure 
GFKAINEVYRKERDSYKLDLCVGKEYQIELFSFPNPPKRPSFPEAAGLRHLAFAVTNIE
CHHHHHHHHHCCCCCEEEEEEECCCEEEEEEECCCCCCCCCCCCHHCHHHHHHHHHHHH
EAVQHLSQCGVETEAIRIDDITGKKFVFFQDPDGLPLELYEV
HHHHHHHHCCCCCCEEEEECCCCCEEEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-S-lactoylglutathione

Specific reaction: (R)-S-lactoylglutathione = glutathione + methylglyoxal

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]