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The map label for this gene is vanS [H]

Identifier: 218901463

GI number: 218901463

Start: 265656

End: 267098

Strand: Direct

Name: vanS [H]

Synonym: BCAH820_0297

Alternate gene names: 218901463

Gene position: 265656-267098 (Clockwise)

Preceding gene: 218901462

Following gene: 218901464

Centisome position: 5.01

GC content: 34.58

Gene sequence:

>1443_bases
ATGAAGAGTGTGAAACGGAGTATACGGATTCAACTTATTACGGCTTTTACTGCTTGTGCGTTATTAGGTCTCTTTGTATC
AACGAAGATAGTAGCGCCTGTTTTTGAAGATGCTAGTCGACATGCTGAGATTAATTATAGAGATGGTATGGAACAAATTA
ATCGTAAAGCGCAAAGTACTGCGGAAATGATGGTTGATGCAAATAAATTAGATGCCGTACAAAGTATGATAGAGCTAGAA
AATGAACATTTAGAGCAGGGGCAGGATGCTTTTAAAATATTAGTTACCGATGAAAATGGTAAGGTTTTGTATAAAACGAA
GCAGGCACAAGAAGAACAAATTAATTTGCATAATACAATTCGTAATGCAACTTCGTTTGCTATCAATTATTCGAATAATA
ATGATATTGAAAGATCTAGAAAAGAGTTTATTACTTTCTCACCTATTACAATAGAAGGTAAGAATTTATATATGTTTGTG
AGTGGAATTCCTCAAGGGGAAGTAGTGTATTATAAAGTAGAAGGGCCATTTCCATTTTTAATTGGTGTACTTGTATTCAT
TTTCTCTTTCTTCTATATAACAAAGAGAAAGATGAAACAAATTGAAGCGATGGCAGAAGGTGTAAAGGAAATAGAAAAAG
GCAACTTAGCGTACCGTATTGAGAAGAAAGGTGAAGATGAGATTGCGTCTTTAACTGAGAATATTAATAATATGGCGGAA
GAGCTTATGAATAATATAGAAAAGGAACGTAGATTAGAGAAGCAAAAGAATGAGCTTATTACGAATGTATCTCACGATTT
ACGTACACCACTGACTTCTATTATGGGTTACTTGCGATTACTTCGAGATTCTAAATATGAAAATAAAGAACAACATGATG
AGTATACAAGAATTGCTTTTGCTAAATCGGAGCAGTTAAAGAATTTAATAGAAGATTTATTTGAGTATACAAAGTTAACG
AATGAACAAGTTGTATTAGAAAAACAAGAAGTATGTGTAAATGAGTTGCTCGAGCAATTAATAGAAGAGTTAGTACCGCA
AGCGGAAGAGCATGGACTTACGTTCGTTAAGAAGTTTCCTGAGGAACGTGCCTATGCAGCGATTGATTCGGAAAAGATGG
TCCGTGTATTTGATAATCTATTAATGAACGCGATTAAGTATAGTAAAGATGATGGGGAAATCAAAGTTTCTCTTCAAAGG
CAGCGCCGGGATATACAAATTGTAATTGCGAATCATAGTGAAGAGTTTACGAGAGAAGAGTTAGCGAGTTTATTTGAACG
CTTTTATAAGAAAGATCAATCTAGAAGTAGGGCAACAGAAGGATCGGGCCTTGGATTGGCGATTGCGAAAAGTATTGTTG
AATTACAAGGTGGTAGTATTCGGGCTGAATATGAGGATGGTATTATTCAGTTTATCGTCTCGTTACCAATTATAGAAAAA
TAA

Upstream 100 bases:

>100_bases
AGATATATAAAAACAGTATGGGGAGTGGGGTATAAGATTGAAAAAGATATTTAATCCATTCACTTATATTAGGAAAATCC
GAGAGTTAATTGGAAAAATA

Downstream 100 bases:

>100_bases
TAAACGAATAGAATGGTATATGATAAAAAGGCTTATTTTAAAAGAAATAAGCTTTTTTATACTTTTTTAGAAAAACATGT
TGACGATTAGAGATTAGAGG

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MKSVKRSIRIQLITAFTACALLGLFVSTKIVAPVFEDASRHAEINYRDGMEQINRKAQSTAEMMVDANKLDAVQSMIELE
NEHLEQGQDAFKILVTDENGKVLYKTKQAQEEQINLHNTIRNATSFAINYSNNNDIERSRKEFITFSPITIEGKNLYMFV
SGIPQGEVVYYKVEGPFPFLIGVLVFIFSFFYITKRKMKQIEAMAEGVKEIEKGNLAYRIEKKGEDEIASLTENINNMAE
ELMNNIEKERRLEKQKNELITNVSHDLRTPLTSIMGYLRLLRDSKYENKEQHDEYTRIAFAKSEQLKNLIEDLFEYTKLT
NEQVVLEKQEVCVNELLEQLIEELVPQAEEHGLTFVKKFPEERAYAAIDSEKMVRVFDNLLMNAIKYSKDDGEIKVSLQR
QRRDIQIVIANHSEEFTREELASLFERFYKKDQSRSRATEGSGLGLAIAKSIVELQGGSIRAEYEDGIIQFIVSLPIIEK

Sequences:

>Translated_480_residues
MKSVKRSIRIQLITAFTACALLGLFVSTKIVAPVFEDASRHAEINYRDGMEQINRKAQSTAEMMVDANKLDAVQSMIELE
NEHLEQGQDAFKILVTDENGKVLYKTKQAQEEQINLHNTIRNATSFAINYSNNNDIERSRKEFITFSPITIEGKNLYMFV
SGIPQGEVVYYKVEGPFPFLIGVLVFIFSFFYITKRKMKQIEAMAEGVKEIEKGNLAYRIEKKGEDEIASLTENINNMAE
ELMNNIEKERRLEKQKNELITNVSHDLRTPLTSIMGYLRLLRDSKYENKEQHDEYTRIAFAKSEQLKNLIEDLFEYTKLT
NEQVVLEKQEVCVNELLEQLIEELVPQAEEHGLTFVKKFPEERAYAAIDSEKMVRVFDNLLMNAIKYSKDDGEIKVSLQR
QRRDIQIVIANHSEEFTREELASLFERFYKKDQSRSRATEGSGLGLAIAKSIVELQGGSIRAEYEDGIIQFIVSLPIIEK
>Mature_480_residues
MKSVKRSIRIQLITAFTACALLGLFVSTKIVAPVFEDASRHAEINYRDGMEQINRKAQSTAEMMVDANKLDAVQSMIELE
NEHLEQGQDAFKILVTDENGKVLYKTKQAQEEQINLHNTIRNATSFAINYSNNNDIERSRKEFITFSPITIEGKNLYMFV
SGIPQGEVVYYKVEGPFPFLIGVLVFIFSFFYITKRKMKQIEAMAEGVKEIEKGNLAYRIEKKGEDEIASLTENINNMAE
ELMNNIEKERRLEKQKNELITNVSHDLRTPLTSIMGYLRLLRDSKYENKEQHDEYTRIAFAKSEQLKNLIEDLFEYTKLT
NEQVVLEKQEVCVNELLEQLIEELVPQAEEHGLTFVKKFPEERAYAAIDSEKMVRVFDNLLMNAIKYSKDDGEIKVSLQR
QRRDIQIVIANHSEEFTREELASLFERFYKKDQSRSRATEGSGLGLAIAKSIVELQGGSIRAEYEDGIIQFIVSLPIIEK

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=281, Percent_Identity=26.3345195729537, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1786783, Length=304, Percent_Identity=25.3289473684211, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788713, Length=249, Percent_Identity=28.5140562248996, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1787894, Length=338, Percent_Identity=28.1065088757396, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1790346, Length=284, Percent_Identity=25.3521126760563, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI145693157, Length=247, Percent_Identity=27.9352226720648, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789149, Length=320, Percent_Identity=25.9375, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786912, Length=281, Percent_Identity=25.9786476868327, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082128, Length=318, Percent_Identity=28.3018867924528, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI48994928, Length=223, Percent_Identity=26.0089686098655, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786600, Length=210, Percent_Identity=27.6190476190476, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788549, Length=283, Percent_Identity=24.3816254416961, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1790436, Length=227, Percent_Identity=26.431718061674, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788279, Length=444, Percent_Identity=23.6486486486486, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789808, Length=298, Percent_Identity=26.1744966442953, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790861, Length=395, Percent_Identity=23.0379746835443, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55308; Mature: 55308

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSVKRSIRIQLITAFTACALLGLFVSTKIVAPVFEDASRHAEINYRDGMEQINRKAQST
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHH
AEMMVDANKLDAVQSMIELENEHLEQGQDAFKILVTDENGKVLYKTKQAQEEQINLHNTI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECHHHHHHHHHHHHH
RNATSFAINYSNNNDIERSRKEFITFSPITIEGKNLYMFVSGIPQGEVVYYKVEGPFPFL
HHHHEEEEECCCCCCHHHHHHHCEEECCEEEECCEEEEEEECCCCCCEEEEEECCCHHHH
IGVLVFIFSFFYITKRKMKQIEAMAEGVKEIEKGNLAYRIEKKGEDEIASLTENINNMAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHH
ELMNNIEKERRLEKQKNELITNVSHDLRTPLTSIMGYLRLLRDSKYENKEQHDEYTRIAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AKSEQLKNLIEDLFEYTKLTNEQVVLEKQEVCVNELLEQLIEELVPQAEEHGLTFVKKFP
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHCC
EERAYAAIDSEKMVRVFDNLLMNAIKYSKDDGEIKVSLQRQRRDIQIVIANHSEEFTREE
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCHHHHHHH
LASLFERFYKKDQSRSRATEGSGLGLAIAKSIVELQGGSIRAEYEDGIIQFIVSLPIIEK
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKSVKRSIRIQLITAFTACALLGLFVSTKIVAPVFEDASRHAEINYRDGMEQINRKAQST
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHH
AEMMVDANKLDAVQSMIELENEHLEQGQDAFKILVTDENGKVLYKTKQAQEEQINLHNTI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECHHHHHHHHHHHHH
RNATSFAINYSNNNDIERSRKEFITFSPITIEGKNLYMFVSGIPQGEVVYYKVEGPFPFL
HHHHEEEEECCCCCCHHHHHHHCEEECCEEEECCEEEEEEECCCCCCEEEEEECCCHHHH
IGVLVFIFSFFYITKRKMKQIEAMAEGVKEIEKGNLAYRIEKKGEDEIASLTENINNMAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHH
ELMNNIEKERRLEKQKNELITNVSHDLRTPLTSIMGYLRLLRDSKYENKEQHDEYTRIAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AKSEQLKNLIEDLFEYTKLTNEQVVLEKQEVCVNELLEQLIEELVPQAEEHGLTFVKKFP
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHCC
EERAYAAIDSEKMVRVFDNLLMNAIKYSKDDGEIKVSLQRQRRDIQIVIANHSEEFTREE
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEECCCHHHHHHH
LASLFERFYKKDQSRSRATEGSGLGLAIAKSIVELQGGSIRAEYEDGIIQFIVSLPIIEK
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]