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The map label for this gene is dus1 [H]

Identifier: 218901278

GI number: 218901278

Start: 79403

End: 80401

Strand: Direct

Name: dus1 [H]

Synonym: BCAH820_0084

Alternate gene names: 218901278

Gene position: 79403-80401 (Clockwise)

Preceding gene: 218901277

Following gene: 218901279

Centisome position: 1.5

GC content: 39.04

Gene sequence:

>999_bases
GTGTTAAAAATCGCAAATATTGAGATGAAAAATCCGGTTGTATTAGCACCGATGGCGGGAGTGTGTAACTCTGCATTCCG
TTTGACAGTAAAAGAATTCGGTGCAGGACTAGTTTGTGCCGAAATGGTAAGTGATAAAGCGATACTATTGAATAATAAAA
GAACATTAGATATGTTATATATCGATGAGAGAGAAAAGCCATTAAGTTTACAAATTTTTGGTGGAGAGAAAGAAACCCTT
GTAGATGCTGCGAAATACGTAGATCAATATACGACAGCAGACATCATTGATATTAATATGGGCTGTCCAGTACCGAAAAT
CGTTAAGTGTGATGCAGGAGCAAAGTGGCTTTTAGACCCAAATAAAATATATGAAATGGTAGCGGCAGTTGTAGATGCTG
TAGAAAAACCAGTTACAGTTAAAATGCGTATTGGTTGGGATGAGGATCATATTTTCGCAATCGAGAATGCGAAAGCTGTT
GAGCGTGCTGGTGGACAAGCGGTAGCAGTTCATGGACGTACAAGAGTACAAATGTATGAAGGAAAAGCGGATTGGGATAT
TATTAAACAAGTAAAGCAAGCTGTAAATATCCCGGTTATCGGAAATGGTGATGTCGCAACGCCACAAGATGCAAAGCGTA
TGCTTGATGAAATTGGTGTAGATGGTGTTATGATTGGCCGCGCTGCCCTTGGAGATCCTTGGATGATTTATCGTACGGTA
AAGTATTTAGAGACAGGTGAATTAATGCCGGAACCGACAGTACGTGAGAAAATTGACGTATGTATGTTGCATCTAGATCG
TCTAATCGATTTAAAGAACGAAAGTATCGCTGTAAGAGAGATGAGAAAGCATGCAGCTTGGTATTTAAAAGGTATTCGTG
GTAATGCAAATGTACGTAATGGTATAAATGCTTGTAATACGCGTGAAGACCTTGCAAATGTATTAGATGTATTTGTAGAA
GAAGTGGAAGCGAAACAACAAACAATTCACGTTGGTTAA

Upstream 100 bases:

>100_bases
GATCGCCTTCCCAAGATTTTGTAGTGGAAGTTGCTAAAGCATTAAAAGTCTCGATAGATGAGCTAATGCCAAAGTAACTA
TGAAGGAAGGAGTAAATCGA

Downstream 100 bases:

>100_bases
TAATAGGGAGGGAAGATTGACATTCTTCTATACACTTCCTATAATTACGTGAAAGAAAGAAGAACTGTCAGTTAGTTGCT
GGCAGTTTTTCTAGTTGAGT

Product: putative TIM-barrel protein, NifR3 family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MLKIANIEMKNPVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKAILLNNKRTLDMLYIDEREKPLSLQIFGGEKETL
VDAAKYVDQYTTADIIDINMGCPVPKIVKCDAGAKWLLDPNKIYEMVAAVVDAVEKPVTVKMRIGWDEDHIFAIENAKAV
ERAGGQAVAVHGRTRVQMYEGKADWDIIKQVKQAVNIPVIGNGDVATPQDAKRMLDEIGVDGVMIGRAALGDPWMIYRTV
KYLETGELMPEPTVREKIDVCMLHLDRLIDLKNESIAVREMRKHAAWYLKGIRGNANVRNGINACNTREDLANVLDVFVE
EVEAKQQTIHVG

Sequences:

>Translated_332_residues
MLKIANIEMKNPVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKAILLNNKRTLDMLYIDEREKPLSLQIFGGEKETL
VDAAKYVDQYTTADIIDINMGCPVPKIVKCDAGAKWLLDPNKIYEMVAAVVDAVEKPVTVKMRIGWDEDHIFAIENAKAV
ERAGGQAVAVHGRTRVQMYEGKADWDIIKQVKQAVNIPVIGNGDVATPQDAKRMLDEIGVDGVMIGRAALGDPWMIYRTV
KYLETGELMPEPTVREKIDVCMLHLDRLIDLKNESIAVREMRKHAAWYLKGIRGNANVRNGINACNTREDLANVLDVFVE
EVEAKQQTIHVG
>Mature_332_residues
MLKIANIEMKNPVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKAILLNNKRTLDMLYIDEREKPLSLQIFGGEKETL
VDAAKYVDQYTTADIIDINMGCPVPKIVKCDAGAKWLLDPNKIYEMVAAVVDAVEKPVTVKMRIGWDEDHIFAIENAKAV
ERAGGQAVAVHGRTRVQMYEGKADWDIIKQVKQAVNIPVIGNGDVATPQDAKRMLDEIGVDGVMIGRAALGDPWMIYRTV
KYLETGELMPEPTVREKIDVCMLHLDRLIDLKNESIAVREMRKHAAWYLKGIRGNANVRNGINACNTREDLANVLDVFVE
EVEAKQQTIHVG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=233, Percent_Identity=30.4721030042918, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI239788462, Length=231, Percent_Identity=30.7359307359307, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI40807366, Length=227, Percent_Identity=31.7180616740088, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI31742496, Length=226, Percent_Identity=30.9734513274336, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI8923374, Length=249, Percent_Identity=28.9156626506024, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1789660, Length=320, Percent_Identity=37.8125, Blast_Score=221, Evalue=3e-59,
Organism=Escherichia coli, GI1788462, Length=253, Percent_Identity=37.1541501976285, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI145693211, Length=227, Percent_Identity=27.7533039647577, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17543114, Length=228, Percent_Identity=31.140350877193, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17507177, Length=243, Percent_Identity=29.6296296296296, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17510279, Length=245, Percent_Identity=29.3877551020408, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI25144369, Length=215, Percent_Identity=27.906976744186, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323560, Length=232, Percent_Identity=30.1724137931034, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323437, Length=229, Percent_Identity=26.6375545851528, Blast_Score=83, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6323433, Length=258, Percent_Identity=25.5813953488372, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24585320, Length=241, Percent_Identity=34.0248962655602, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24580595, Length=252, Percent_Identity=34.1269841269841, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19920448, Length=252, Percent_Identity=34.1269841269841, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI45549423, Length=240, Percent_Identity=30, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19921524, Length=306, Percent_Identity=25.4901960784314, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36860; Mature: 36860

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKIANIEMKNPVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKAILLNNKRTLDMLY
CEEEEEEECCCCEEEECHHHHCCHHHEEEHHHHCCCCEEHHHHCCCEEEEECCCEEEEEE
IDEREKPLSLQIFGGEKETLVDAAKYVDQYTTADIIDINMGCPVPKIVKCDAGAKWLLDP
ECCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCEEEECH
NKIYEMVAAVVDAVEKPVTVKMRIGWDEDHIFAIENAKAVERAGGQAVAVHGRTRVQMYE
HHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCCHHHHHCCCCEEEEECCEEEEEEC
GKADWDIIKQVKQAVNIPVIGNGDVATPQDAKRMLDEIGVDGVMIGRAALGDPWMIYRTV
CCCCHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHCCCEEEECCHHCCCCHHHHHHH
KYLETGELMPEPTVREKIDVCMLHLDRLIDLKNESIAVREMRKHAAWYLKGIRGNANVRN
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
GINACNTREDLANVLDVFVEEVEAKQQTIHVG
CCCCCCCHHHHHHHHHHHHHHHHHHHHEEECC
>Mature Secondary Structure
MLKIANIEMKNPVVLAPMAGVCNSAFRLTVKEFGAGLVCAEMVSDKAILLNNKRTLDMLY
CEEEEEEECCCCEEEECHHHHCCHHHEEEHHHHCCCCEEHHHHCCCEEEEECCCEEEEEE
IDEREKPLSLQIFGGEKETLVDAAKYVDQYTTADIIDINMGCPVPKIVKCDAGAKWLLDP
ECCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCEEEECH
NKIYEMVAAVVDAVEKPVTVKMRIGWDEDHIFAIENAKAVERAGGQAVAVHGRTRVQMYE
HHHHHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCCHHHHHCCCCEEEEECCEEEEEEC
GKADWDIIKQVKQAVNIPVIGNGDVATPQDAKRMLDEIGVDGVMIGRAALGDPWMIYRTV
CCCCHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHCCCEEEECCHHCCCCHHHHHHH
KYLETGELMPEPTVREKIDVCMLHLDRLIDLKNESIAVREMRKHAAWYLKGIRGNANVRN
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
GINACNTREDLANVLDVFVEEVEAKQQTIHVG
CCCCCCCHHHHHHHHHHHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]