Click here to switch to the map view.

The map label for this gene is lysS [H]

Identifier: 218901279

GI number: 218901279

Start: 80561

End: 82060

Strand: Direct

Name: lysS [H]

Synonym: BCAH820_0085

Alternate gene names: 218901279

Gene position: 80561-82060 (Clockwise)

Preceding gene: 218901278

Following gene: 218901280

Centisome position: 1.52

GC content: 36.67

Gene sequence:

>1500_bases
ATGGATAACATGAACCACGAAGAATTAAACGACCAATTGCTTGTTCGTCGTGAAAAGCTACATAACTTACGTGAGCAAGG
AATCGATCCTTTCGGTAAACGATTTGAACGTACAAATGCAACAAATGACTTATTAAGCCTATATGGAGAGTTCTCTAAAG
AAGAATTAGAAGAGAAAGAAATCTCTGTTTCTATCGCTGGTCGTATTATGACAAAGCGTGGTAAAGGAAAAGCTGGATTT
GCACATATTCAAGATTTACATGGACAAGTTCAAATTTATGTTCGTAAAGATGCTGTTGGAGATGAAGAATACGAATTATT
TAAAACAGCAGATTTAGGTGACTTAGTAGGTATTGAAGGTAAAGTTTTTAAAACGAACGTTGGAGAACTTTCAGTGAAAG
CAACAGGCTTTACACTATTAACGAAATCTCTTCGTCCATTACCGGATAAATATCATGGGTTAAAAGATGTTGAACAACGC
TACCGTCAACGTTACTTAGACTTAATTACAAGTATGGAAAGTCGTGAAACATTCGTCACTCGCAGTAAGATCATTCGCGA
AATGAGAAGATACTTAGATGACAACGGTTATCTTGAAGTGGAAACACCTATGATGCATGCAATTGCAGGTGGAGCTTCTG
CTCGTCCTTTCATCACGCATCATAATGCGTTAGATATGGAATTATATATGCGTATCGCAATTGAACTTCATTTAAAACGC
CTTATTGTGGGTGGATTAGAAAAAGTTTATGAAATCGGCCGTGTATTCCGTAATGAGGGTGTATCAACTCGTCATAATCC
TGAATTTACGATGATTGAATTATATGAAGCGTATGCTGATTATAAAGATATTATGAAACTAACAGAAAATATGGTTGCTC
ATATCGCGAAGCAAGTACTAGGTACAACAACAATCCAATATGGTGATTATGAAATTAATCTAGAACCAAAATGGACACGC
CTTCATATGGTAGATGCAATTAAAGAACATTCTGGAGCAGATTTCTGGAATCCGATGAGTGTAGAAGAAGCGCGTGAACT
TGCAAAAGAACATAATGTAGAAATTAAGGATACAATGGAAGTTGGTCATATTATTAATGAATTCTTCGAGCAAAAAGTAG
AAGATAAATTAATCCAACCAACATTTATTTACGGTCATCCGGTAGAAATTTCGCCACTTGCGAAAAAGAATGATGAAGAT
CCACGATTCACAGATCGTTTCGAATTGTTTATCGTTGCACGTGAACATGCAAATGCGTTCACTGAGCTAAATGATCCAAT
CGATCAGAAAGAACGTTTTGAAGCGCAATTAAAAGAACGCGAACAAGGTAATGACGAAGCTCACATGATGGATGATGATT
ATATCGAAGCTCTTGAGTATGGTATGCCTCCTACAGGTGGACTAGGAATTGGTATTGATCGCCTTGTTATGTTATTAACG
AATGCACCATCTATTCGTGATGTATTATTATTCCCTGCTATGCGTCATAAACAAGACTAA

Upstream 100 bases:

>100_bases
AAGAACTGTCAGTTAGTTGCTGGCAGTTTTTCTAGTTGAGTAGAAAATGCTATACTGTATATATTGTAAAAATTAATAGA
GCTGGAGTGATATCAATACC

Downstream 100 bases:

>100_bases
GCTTTGGAGATTTCTCCAAAGCTTTTTTTATTTGGGTGTGAACAGCTTGGAGATAGTAAATCTTGATAGTGCGAAAAATT
ATATTTATATAGAAGAAAAG

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MDNMNHEELNDQLLVRREKLHNLREQGIDPFGKRFERTNATNDLLSLYGEFSKEELEEKEISVSIAGRIMTKRGKGKAGF
AHIQDLHGQVQIYVRKDAVGDEEYELFKTADLGDLVGIEGKVFKTNVGELSVKATGFTLLTKSLRPLPDKYHGLKDVEQR
YRQRYLDLITSMESRETFVTRSKIIREMRRYLDDNGYLEVETPMMHAIAGGASARPFITHHNALDMELYMRIAIELHLKR
LIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMKLTENMVAHIAKQVLGTTTIQYGDYEINLEPKWTR
LHMVDAIKEHSGADFWNPMSVEEARELAKEHNVEIKDTMEVGHIINEFFEQKVEDKLIQPTFIYGHPVEISPLAKKNDED
PRFTDRFELFIVAREHANAFTELNDPIDQKERFEAQLKEREQGNDEAHMMDDDYIEALEYGMPPTGGLGIGIDRLVMLLT
NAPSIRDVLLFPAMRHKQD

Sequences:

>Translated_499_residues
MDNMNHEELNDQLLVRREKLHNLREQGIDPFGKRFERTNATNDLLSLYGEFSKEELEEKEISVSIAGRIMTKRGKGKAGF
AHIQDLHGQVQIYVRKDAVGDEEYELFKTADLGDLVGIEGKVFKTNVGELSVKATGFTLLTKSLRPLPDKYHGLKDVEQR
YRQRYLDLITSMESRETFVTRSKIIREMRRYLDDNGYLEVETPMMHAIAGGASARPFITHHNALDMELYMRIAIELHLKR
LIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMKLTENMVAHIAKQVLGTTTIQYGDYEINLEPKWTR
LHMVDAIKEHSGADFWNPMSVEEARELAKEHNVEIKDTMEVGHIINEFFEQKVEDKLIQPTFIYGHPVEISPLAKKNDED
PRFTDRFELFIVAREHANAFTELNDPIDQKERFEAQLKEREQGNDEAHMMDDDYIEALEYGMPPTGGLGIGIDRLVMLLT
NAPSIRDVLLFPAMRHKQD
>Mature_499_residues
MDNMNHEELNDQLLVRREKLHNLREQGIDPFGKRFERTNATNDLLSLYGEFSKEELEEKEISVSIAGRIMTKRGKGKAGF
AHIQDLHGQVQIYVRKDAVGDEEYELFKTADLGDLVGIEGKVFKTNVGELSVKATGFTLLTKSLRPLPDKYHGLKDVEQR
YRQRYLDLITSMESRETFVTRSKIIREMRRYLDDNGYLEVETPMMHAIAGGASARPFITHHNALDMELYMRIAIELHLKR
LIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMKLTENMVAHIAKQVLGTTTIQYGDYEINLEPKWTR
LHMVDAIKEHSGADFWNPMSVEEARELAKEHNVEIKDTMEVGHIINEFFEQKVEDKLIQPTFIYGHPVEISPLAKKNDED
PRFTDRFELFIVAREHANAFTELNDPIDQKERFEAQLKEREQGNDEAHMMDDDYIEALEYGMPPTGGLGIGIDRLVMLLT
NAPSIRDVLLFPAMRHKQD

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=504, Percent_Identity=41.6666666666667, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI5031815, Length=504, Percent_Identity=41.6666666666667, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI40789249, Length=281, Percent_Identity=24.5551601423488, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1789256, Length=490, Percent_Identity=52.8571428571429, Blast_Score=527, Evalue=1e-151,
Organism=Escherichia coli, GI1790571, Length=493, Percent_Identity=52.1298174442191, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI87082379, Length=325, Percent_Identity=33.2307692307692, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1788173, Length=296, Percent_Identity=26.0135135135135, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17535925, Length=491, Percent_Identity=46.4358452138493, Blast_Score=430, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17535927, Length=491, Percent_Identity=46.4358452138493, Blast_Score=429, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI71994340, Length=491, Percent_Identity=46.0285132382892, Blast_Score=428, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17551876, Length=508, Percent_Identity=22.244094488189, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI32566633, Length=271, Percent_Identity=23.6162361623616, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6320242, Length=510, Percent_Identity=42.7450980392157, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324256, Length=483, Percent_Identity=32.2981366459627, Blast_Score=209, Evalue=6e-55,
Organism=Saccharomyces cerevisiae, GI6323011, Length=350, Percent_Identity=24.8571428571429, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6321807, Length=323, Percent_Identity=26.3157894736842, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640849, Length=505, Percent_Identity=41.980198019802, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24640851, Length=505, Percent_Identity=41.980198019802, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24584738, Length=269, Percent_Identity=26.3940520446097, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922416, Length=376, Percent_Identity=23.6702127659574, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57680; Mature: 57680

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNMNHEELNDQLLVRREKLHNLREQGIDPFGKRFERTNATNDLLSLYGEFSKEELEEKE
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ISVSIAGRIMTKRGKGKAGFAHIQDLHGQVQIYVRKDAVGDEEYELFKTADLGDLVGIEG
HHHEEHHHHHCCCCCCCCCHHHHHHCCCEEEEEEEECCCCCCHHHHHCCCCCCHHCCCCC
KVFKTNVGELSVKATGFTLLTKSLRPLPDKYHGLKDVEQRYRQRYLDLITSMESRETFVT
EEEEECCCCEEEEECCHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSKIIREMRRYLDDNGYLEVETPMMHAIAGGASARPFITHHNALDMELYMRIAIELHLKR
HHHHHHHHHHHHCCCCEEEECCHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHH
LIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMKLTENMVAHIAKQVL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTTTIQYGDYEINLEPKWTRLHMVDAIKEHSGADFWNPMSVEEARELAKEHNVEIKDTME
CCCEEEECCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHH
VGHIINEFFEQKVEDKLIQPTFIYGHPVEISPLAKKNDEDPRFTDRFELFIVAREHANAF
HHHHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCCCCCCCCCCCEEEEEEEEEHHCCHH
TELNDPIDQKERFEAQLKEREQGNDEAHMMDDDYIEALEYGMPPTGGLGIGIDRLVMLLT
HHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
NAPSIRDVLLFPAMRHKQD
CCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDNMNHEELNDQLLVRREKLHNLREQGIDPFGKRFERTNATNDLLSLYGEFSKEELEEKE
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
ISVSIAGRIMTKRGKGKAGFAHIQDLHGQVQIYVRKDAVGDEEYELFKTADLGDLVGIEG
HHHEEHHHHHCCCCCCCCCHHHHHHCCCEEEEEEEECCCCCCHHHHHCCCCCCHHCCCCC
KVFKTNVGELSVKATGFTLLTKSLRPLPDKYHGLKDVEQRYRQRYLDLITSMESRETFVT
EEEEECCCCEEEEECCHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSKIIREMRRYLDDNGYLEVETPMMHAIAGGASARPFITHHNALDMELYMRIAIELHLKR
HHHHHHHHHHHHCCCCEEEECCHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHH
LIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMKLTENMVAHIAKQVL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GTTTIQYGDYEINLEPKWTRLHMVDAIKEHSGADFWNPMSVEEARELAKEHNVEIKDTME
CCCEEEECCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHH
VGHIINEFFEQKVEDKLIQPTFIYGHPVEISPLAKKNDEDPRFTDRFELFIVAREHANAF
HHHHHHHHHHHHHHHHHCCCEEEECCCCEECCCCCCCCCCCCCCCEEEEEEEEEHHCCHH
TELNDPIDQKERFEAQLKEREQGNDEAHMMDDDYIEALEYGMPPTGGLGIGIDRLVMLLT
HHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
NAPSIRDVLLFPAMRHKQD
CCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA