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The map label for this gene is yaaI [H]

Identifier: 218884761

GI number: 218884761

Start: 1343840

End: 1344394

Strand: Reverse

Name: yaaI [H]

Synonym: DKAM_1450

Alternate gene names: 218884761

Gene position: 1344394-1343840 (Counterclockwise)

Preceding gene: 218884763

Following gene: 218884760

Centisome position: 98.47

GC content: 43.96

Gene sequence:

>555_bases
GTGTCGCTCTTGAGGCCGGCTCTACTAGTAGTGGACATGTTGAAAGAGTTTGTACATGGTAGACTAAGAAGCCCGGAGGC
ATCGAGAATAATCCCTGTGATAAAGGATCTAACGAGCATCATGCATAAAAGAGGATTCCCAGTTATATACCTAGCCGATC
ACCACTACCCATTCGATCATGAGTTATCCATATGGGGACCGCATGCAATGCATAATGATCCAGAGTCGGAGATAATAGAG
GAGCTGAGGCCTGGACCAAGCGATATAGTATTATATAAGAGATCCTATAGCGGCTTCAGGGAAACAGGCCTAGACTACAT
ACTCAGAGACCTAGGCATAGACACCGTGATTATAACAGGTATACATACTCATATATGCGTACTCCACACGGCAATGGATG
CATTCTATAACAGGTACCAGGTTATAGTGGTCGAGGATGCAGTGAGCGCTTTTAGTAGAAGCGACCACGAATGGGCATTA
AACTATATGAAGACCATACTTGGATGCAGAATAATGAAGTCCAGGGAAGTAATAGAATTCATAAGCAGCAAATAG

Upstream 100 bases:

>100_bases
AATATCAGCCCTCACACGGGTCTTCATCGCTTTAACTCAGAACCGAAGTCGGTCATCACACTAACTTTTAATTCCCTCAA
CCACCTTATATCAATAACTG

Downstream 100 bases:

>100_bases
TGGTTAACAGTGATCAACTGCGGCTTACTGGAAACAATTAAGAATATTTCAAGGGAATCGGGATTCACTGTAGCAGAGCT
ATTAGTAGTCATGCTGGGAA

Product: isochorismatase hydrolase

Products: 2,3-Dihydro-2,3-dihydroxybenzoate; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 183

Protein sequence:

>184_residues
MSLLRPALLVVDMLKEFVHGRLRSPEASRIIPVIKDLTSIMHKRGFPVIYLADHHYPFDHELSIWGPHAMHNDPESEIIE
ELRPGPSDIVLYKRSYSGFRETGLDYILRDLGIDTVIITGIHTHICVLHTAMDAFYNRYQVIVVEDAVSAFSRSDHEWAL
NYMKTILGCRIMKSREVIEFISSK

Sequences:

>Translated_184_residues
MSLLRPALLVVDMLKEFVHGRLRSPEASRIIPVIKDLTSIMHKRGFPVIYLADHHYPFDHELSIWGPHAMHNDPESEIIE
ELRPGPSDIVLYKRSYSGFRETGLDYILRDLGIDTVIITGIHTHICVLHTAMDAFYNRYQVIVVEDAVSAFSRSDHEWAL
NYMKTILGCRIMKSREVIEFISSK
>Mature_183_residues
SLLRPALLVVDMLKEFVHGRLRSPEASRIIPVIKDLTSIMHKRGFPVIYLADHHYPFDHELSIWGPHAMHNDPESEIIEE
LRPGPSDIVLYKRSYSGFRETGLDYILRDLGIDTVIITGIHTHICVLHTAMDAFYNRYQVIVVEDAVSAFSRSDHEWALN
YMKTILGCRIMKSREVIEFISSK

Specific function: Required For Production Of 2,3-Dhb. Also Serves As An Aryl Carrier Protein And Plays A Role In Enterobactin Assembly. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family [H]

Homologues:

Organism=Escherichia coli, GI1786811, Length=180, Percent_Identity=31.1111111111111, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI87081820, Length=93, Percent_Identity=43.010752688172, Blast_Score=71, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: 3.3.2.1 [C]

Molecular weight: Translated: 21205; Mature: 21074

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLRPALLVVDMLKEFVHGRLRSPEASRIIPVIKDLTSIMHKRGFPVIYLADHHYPFDH
CCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
ELSIWGPHAMHNDPESEIIEELRPGPSDIVLYKRSYSGFRETGLDYILRDLGIDTVIITG
CEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEHH
IHTHICVLHTAMDAFYNRYQVIVVEDAVSAFSRSDHEWALNYMKTILGCRIMKSREVIEF
HHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ISSK
HHCC
>Mature Secondary Structure 
SLLRPALLVVDMLKEFVHGRLRSPEASRIIPVIKDLTSIMHKRGFPVIYLADHHYPFDH
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
ELSIWGPHAMHNDPESEIIEELRPGPSDIVLYKRSYSGFRETGLDYILRDLGIDTVIITG
CEEECCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEHH
IHTHICVLHTAMDAFYNRYQVIVVEDAVSAFSRSDHEWALNYMKTILGCRIMKSREVIEF
HHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ISSK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; Isochorismate [C]

Specific reaction: H2O + Isochorismate --> 2,3-Dihydro-2,3-dihydroxybenzoate + Pyruvate [C]

General reaction: Ether hydrolysis [C]

Inhibitor: Fe2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]