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The map label for this gene is gltX [H]

Identifier: 218884665

GI number: 218884665

Start: 1259001

End: 1260737

Strand: Reverse

Name: gltX [H]

Synonym: DKAM_1354

Alternate gene names: 218884665

Gene position: 1260737-1259001 (Counterclockwise)

Preceding gene: 218884666

Following gene: 218884664

Centisome position: 92.35

GC content: 43.24

Gene sequence:

>1737_bases
GTGAGAGAAACCGCTTTCAAACACGCATTGGTAAACGCTGTAAAACACGAGGGTAAAGCCGATTTTAAAGCGGTGGTTTC
AAAGGTTATCGGGGAAATCCCAGAGATACGTAGCAGAATCAAAGAATTCATCGACTTGATAAAGGGCACCATAGAGGAAG
TAAACAAGTTAAGCATCGAAGACCAGTTGAAAATAATCAGAGAGAACTGGCCAGAACTACTGGAGGAGAAGAGGGAAGCC
AAGGAGAAGGAACTGCCCCCATTACCCAATGCCGAGAAAGGTAAAGTGGTAACCAGGTTTGCACCTAACCCAGATTACAC
AATTCACCTTGGAAACGCTAGGCCAGCGCTGTTAAGCTACTGGTATGCTGAATTATATAATGGAGCGATGATACTTCGTT
TCGAGGACACCGACCCAAGGATAAAATCACCTTACCCAGAAGCATATACAATGATAAAGGACGCATTGAAATGGCTTGGA
GTAAAATGGAGCAAGGAATACATACAGAGCCTCCGGATGAATATATTCTACAATGTAGCAAGAGAGCTGATAAGCCGTGG
AGGAGCATATATTGACACCTGCCCCGATAAGGAATTCCGCTTATATAGGAACGCTGGCAAGGCATGTCCCCATAGGAATA
CGTCAGTCGAAGAGAACCTTGAGAAATTCGATAAAATGCTTGAGGGGCACTATGGTGAAGGAGATGCTGTTCTAAGGGTG
AAAACCGATTTACAACACCCGGATCCAAGCGTAAGGGATTGGGTTGCATTCCGTGTAATAGATACATCTAAAACACCACA
CCCGGTCACCGGGGAGAAATACATTGTATGGCCAACTTACAATTTTGCTGCTGGTGTCGACGATTATTTAATGGGTGTAA
CACATGTCCTCCGCGCAAAGGAACACATATCGAATACTGTCAAGCAGAAATACCTATATAAGCACATGGGCTGGAAATAC
CCCGAGACAATACATTTTGGACGTTTATCATTAGAGGGTGTGATACTGAGCAAGTCTAAAATGAGAAAGCTAGTTAAAGA
ACAAGGTATCTCACCATATGATGACCCAAGACTCGGAACAATTAATGGGTTACGTAGAAGAGGCATTCTCCGTGAAACAA
TCTGGAAAATAGTTAAGGAAGTGGGAATCAAACCCATCGATGCCAAGATAAGCCTAGTTAATCTCTACGCTATCAACAGG
ATCCTAGTTGATCCAGTCGCGAACAGGTATATGGGTGTTGAAGAACCGGTTCCAGTAATACTAAAGGATGTGAAAGAGGA
GCTTAAAGCACAAATACCAATACATCCATCAAGACCGGGGCATTACGAGTATAGGATACTGGATGGCTCGGTAATCCATA
TATCATCCAAAGACTTGGGAACACTGCTTCAAAGCGAACATAAAACATTCAGAGCCATGGGGCTAGCTAACTTTACGATA
ACGGAGCCAACGATATTGGATGGTAAGCCAGCATTCATAGCCAGGCTCCACAGTATTTCACAGGAAGATGCAAGGAAGCT
AAATGCTCCAATAATTCAATGGGTTAGCAACGATGAGAAAACAGATTTATTCCTCCTGATCCCGAGTGGATCAGAGATCA
GGGAAGCCAGGCTCCTAGTTGAAAAACGCATTATTAATGAAAAACCAGACTCCATTGTGCAATTATACAGGGTCGGGTTC
ATTAGAATAGATACTATCAAAGAGGAAAAAGTAATAGCCGTCTTCTCACACGAATAA

Upstream 100 bases:

>100_bases
GGATTTATACTAGCAGTCATCGATTTAACCTGGGAGAGGCCTGAGAGAATCATAGTGGTGGTGGCGATTGAGCAGCCAAG
AGAAATCATTATTAGATAGG

Downstream 100 bases:

>100_bases
GACCACCGGATTCATTATTATATGGAAATGGAAAGTCGCATCAAAAGTAGTTTAAAAATTATTTAATGCCCGTAATTTAT
AAAGCCCTGACATTATTCTA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MRETAFKHALVNAVKHEGKADFKAVVSKVIGEIPEIRSRIKEFIDLIKGTIEEVNKLSIEDQLKIIRENWPELLEEKREA
KEKELPPLPNAEKGKVVTRFAPNPDYTIHLGNARPALLSYWYAELYNGAMILRFEDTDPRIKSPYPEAYTMIKDALKWLG
VKWSKEYIQSLRMNIFYNVARELISRGGAYIDTCPDKEFRLYRNAGKACPHRNTSVEENLEKFDKMLEGHYGEGDAVLRV
KTDLQHPDPSVRDWVAFRVIDTSKTPHPVTGEKYIVWPTYNFAAGVDDYLMGVTHVLRAKEHISNTVKQKYLYKHMGWKY
PETIHFGRLSLEGVILSKSKMRKLVKEQGISPYDDPRLGTINGLRRRGILRETIWKIVKEVGIKPIDAKISLVNLYAINR
ILVDPVANRYMGVEEPVPVILKDVKEELKAQIPIHPSRPGHYEYRILDGSVIHISSKDLGTLLQSEHKTFRAMGLANFTI
TEPTILDGKPAFIARLHSISQEDARKLNAPIIQWVSNDEKTDLFLLIPSGSEIREARLLVEKRIINEKPDSIVQLYRVGF
IRIDTIKEEKVIAVFSHE

Sequences:

>Translated_578_residues
MRETAFKHALVNAVKHEGKADFKAVVSKVIGEIPEIRSRIKEFIDLIKGTIEEVNKLSIEDQLKIIRENWPELLEEKREA
KEKELPPLPNAEKGKVVTRFAPNPDYTIHLGNARPALLSYWYAELYNGAMILRFEDTDPRIKSPYPEAYTMIKDALKWLG
VKWSKEYIQSLRMNIFYNVARELISRGGAYIDTCPDKEFRLYRNAGKACPHRNTSVEENLEKFDKMLEGHYGEGDAVLRV
KTDLQHPDPSVRDWVAFRVIDTSKTPHPVTGEKYIVWPTYNFAAGVDDYLMGVTHVLRAKEHISNTVKQKYLYKHMGWKY
PETIHFGRLSLEGVILSKSKMRKLVKEQGISPYDDPRLGTINGLRRRGILRETIWKIVKEVGIKPIDAKISLVNLYAINR
ILVDPVANRYMGVEEPVPVILKDVKEELKAQIPIHPSRPGHYEYRILDGSVIHISSKDLGTLLQSEHKTFRAMGLANFTI
TEPTILDGKPAFIARLHSISQEDARKLNAPIIQWVSNDEKTDLFLLIPSGSEIREARLLVEKRIINEKPDSIVQLYRVGF
IRIDTIKEEKVIAVFSHE
>Mature_578_residues
MRETAFKHALVNAVKHEGKADFKAVVSKVIGEIPEIRSRIKEFIDLIKGTIEEVNKLSIEDQLKIIRENWPELLEEKREA
KEKELPPLPNAEKGKVVTRFAPNPDYTIHLGNARPALLSYWYAELYNGAMILRFEDTDPRIKSPYPEAYTMIKDALKWLG
VKWSKEYIQSLRMNIFYNVARELISRGGAYIDTCPDKEFRLYRNAGKACPHRNTSVEENLEKFDKMLEGHYGEGDAVLRV
KTDLQHPDPSVRDWVAFRVIDTSKTPHPVTGEKYIVWPTYNFAAGVDDYLMGVTHVLRAKEHISNTVKQKYLYKHMGWKY
PETIHFGRLSLEGVILSKSKMRKLVKEQGISPYDDPRLGTINGLRRRGILRETIWKIVKEVGIKPIDAKISLVNLYAINR
ILVDPVANRYMGVEEPVPVILKDVKEELKAQIPIHPSRPGHYEYRILDGSVIHISSKDLGTLLQSEHKTFRAMGLANFTI
TEPTILDGKPAFIARLHSISQEDARKLNAPIIQWVSNDEKTDLFLLIPSGSEIREARLLVEKRIINEKPDSIVQLYRVGF
IRIDTIKEEKVIAVFSHE

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=394, Percent_Identity=30.7106598984772, Blast_Score=190, Evalue=4e-48,
Organism=Homo sapiens, GI4826960, Length=501, Percent_Identity=28.1437125748503, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI134288884, Length=278, Percent_Identity=30.9352517985612, Blast_Score=109, Evalue=8e-24,
Organism=Escherichia coli, GI1786895, Length=359, Percent_Identity=34.2618384401114, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1788743, Length=269, Percent_Identity=28.2527881040892, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI226510925, Length=289, Percent_Identity=28.3737024221453, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17511007, Length=507, Percent_Identity=26.232741617357, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17543332, Length=512, Percent_Identity=27.5390625, Blast_Score=161, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI71993394, Length=294, Percent_Identity=29.5918367346939, Blast_Score=95, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324742, Length=403, Percent_Identity=32.7543424317618, Blast_Score=215, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6321192, Length=404, Percent_Identity=30.4455445544554, Blast_Score=184, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6324540, Length=324, Percent_Identity=30.2469135802469, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24649466, Length=409, Percent_Identity=29.5843520782396, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI17864488, Length=502, Percent_Identity=29.8804780876494, Blast_Score=178, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24665321, Length=416, Percent_Identity=30.2884615384615, Blast_Score=109, Evalue=5e-24,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004526
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR022888
- InterPro:   IPR011035
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 66390; Mature: 66390

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRETAFKHALVNAVKHEGKADFKAVVSKVIGEIPEIRSRIKEFIDLIKGTIEEVNKLSIE
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHH
DQLKIIRENWPELLEEKREAKEKELPPLPNAEKGKVVTRFAPNPDYTIHLGNARPALLSY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHH
WYAELYNGAMILRFEDTDPRIKSPYPEAYTMIKDALKWLGVKWSKEYIQSLRMNIFYNVA
HHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RELISRGGAYIDTCPDKEFRLYRNAGKACPHRNTSVEENLEKFDKMLEGHYGEGDAVLRV
HHHHHCCCCEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEE
KTDLQHPDPSVRDWVAFRVIDTSKTPHPVTGEKYIVWPTYNFAAGVDDYLMGVTHVLRAK
EECCCCCCCCHHHEEEEEEEECCCCCCCCCCCEEEEEECCCHHCCHHHHHHHHHHHHHHH
EHISNTVKQKYLYKHMGWKYPETIHFGRLSLEGVILSKSKMRKLVKEQGISPYDDPRLGT
HHHHHHHHHHHHHHHCCCCCCCEEEECCEEECEEEECHHHHHHHHHHCCCCCCCCCCCCC
INGLRRRGILRETIWKIVKEVGIKPIDAKISLVNLYAINRILVDPVANRYMGVEEPVPVI
HHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHH
LKDVKEELKAQIPIHPSRPGHYEYRILDGSVIHISSKDLGTLLQSEHKTFRAMGLANFTI
HHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEE
TEPTILDGKPAFIARLHSISQEDARKLNAPIIQWVSNDEKTDLFLLIPSGSEIREARLLV
CCCEEECCCHHHHHHHHHCCHHHHHHCCCHHHHHCCCCCCCEEEEEECCCCHHHHHHHHH
EKRIINEKPDSIVQLYRVGFIRIDTIKEEKVIAVFSHE
HHHHHCCCCHHHHHHHHHCEEEEEEECCCCEEEEEECC
>Mature Secondary Structure
MRETAFKHALVNAVKHEGKADFKAVVSKVIGEIPEIRSRIKEFIDLIKGTIEEVNKLSIE
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHH
DQLKIIRENWPELLEEKREAKEKELPPLPNAEKGKVVTRFAPNPDYTIHLGNARPALLSY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHH
WYAELYNGAMILRFEDTDPRIKSPYPEAYTMIKDALKWLGVKWSKEYIQSLRMNIFYNVA
HHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RELISRGGAYIDTCPDKEFRLYRNAGKACPHRNTSVEENLEKFDKMLEGHYGEGDAVLRV
HHHHHCCCCEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEE
KTDLQHPDPSVRDWVAFRVIDTSKTPHPVTGEKYIVWPTYNFAAGVDDYLMGVTHVLRAK
EECCCCCCCCHHHEEEEEEEECCCCCCCCCCCEEEEEECCCHHCCHHHHHHHHHHHHHHH
EHISNTVKQKYLYKHMGWKYPETIHFGRLSLEGVILSKSKMRKLVKEQGISPYDDPRLGT
HHHHHHHHHHHHHHHCCCCCCCEEEECCEEECEEEECHHHHHHHHHHCCCCCCCCCCCCC
INGLRRRGILRETIWKIVKEVGIKPIDAKISLVNLYAINRILVDPVANRYMGVEEPVPVI
HHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHHH
LKDVKEELKAQIPIHPSRPGHYEYRILDGSVIHISSKDLGTLLQSEHKTFRAMGLANFTI
HHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEE
TEPTILDGKPAFIARLHSISQEDARKLNAPIIQWVSNDEKTDLFLLIPSGSEIREARLLV
CCCEEECCCHHHHHHHHHCCHHHHHHCCCHHHHHCCCCCCCEEEEEECCCCHHHHHHHHH
EKRIINEKPDSIVQLYRVGFIRIDTIKEEKVIAVFSHE
HHHHHCCCCHHHHHHHHHCEEEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA