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The map label for this gene is eif2g [H]

Identifier: 218884658

GI number: 218884658

Start: 1253129

End: 1254385

Strand: Reverse

Name: eif2g [H]

Synonym: DKAM_1347

Alternate gene names: 218884658

Gene position: 1254385-1253129 (Counterclockwise)

Preceding gene: 218884659

Following gene: 218884657

Centisome position: 91.88

GC content: 47.02

Gene sequence:

>1257_bases
ATGCCGTGGGATATTAAGCCACCCGAGGTCAACATAGGCGTCGTTGGACACGTTGACCACGGTAAAACCACTCTGGTCCA
AGCATTGACGGGTGTCTGGACAGCAAAGCACAGCCTAGAGATAGAACGTGGAATGACTATTAAACTAGGATATGCTGATG
GGAACATCGCCTATTGTGATGGGCTACCGCTACCTGACGCATACACGATTAAAGAGTATTGTCCAGACGGCTCTGAGTCG
AAGTTGCTTAGAAGGGTTAGCTACGTTGACGCGCCTGGTCATGAATTATATATGACGACCATGTTAAGTGGGGCAATGAT
CATGGATGGAGCTATATTAGTTATCGCGGCCAACGAGCCATGTCCTCAGCCACAGACGGTAGAACACTTGATGGCGTTGA
ACATCCTCGGTTTACGGAACATCATCATTGTTCAGAACAAAATAGATGTCGTCGATAAAAAACGCGCATTAGAGAACTAT
AACGAGATCTTAAAGCTGGTTAAAGGCACTGTAGCCGAGAACGCACCAATAATACCGGTCAGCGCCCTACACCACATCAA
CATAGATGCGTTGCTCCAAGCTATACAAGAGATCATTCCAACACCTGAGAGAGACTACACGAAGCCTCCATTAATGTATG
TTGTGAGAAGCTTTGATGTAAACAAGCCCGGCACCCCCTATGATAAAATAGAGGGAGGAGTAATCGGGGGCTCCCTTCTA
CAAGGGCGTTTCAGAGTAGGAGATGAGATAATGATACTCCCCGGGATAAAGACGCAAGTAGGGAGTGCTAAATCACAGGT
CTCTAAATACGAGCCTATAGTGACGACTATAGAGGAGGTACGCTATGGCAACTTAATAGTGGATGAGGCCAGACCTGGAG
GACTGGTGGCAATACAGACCAAGCTGGATCCCTCGCTAACTAAAGGTGATCAATTAGTGGGTTCAATAGTAACCACTCCT
AGGAACAATATACCAGTTGTGAAGACCTTGGAGGTGCGCTTCCAGTTGTTCGAGAGAATAGTGGGCACCAAAGAACTAGG
GAAACTCCCACCAATACAGGTGAATGAGAAAATAGCCGTTGCAATCGGTACAGCGAACAGGGTAGCCGTAGTGAAGTCTA
TAAGGAAAGATATAATGACCATAGAGCTAACAGACCCTGTTGCAACATGGAATGGATCCAGGATAGCTATAAGCCGCCGT
GTATTAGCAAGGTGGAGGCTTGCTGGCTGGGGGATAATAGAGGGTGTCTCAGAATAA

Upstream 100 bases:

>100_bases
TTATTTTTAACCATGGCAGTCTCTGCACATGAGGATTGGGCGGCTTGGATTTATAAATGAATCTAAACTAAATTATTCTA
GCTAAGGTGAATAAGTAATC

Downstream 100 bases:

>100_bases
GGGGATAACTGTTTTACTCGATACAAACATGCTTCTCATGATGGCTAACGGCATACCTGTACTTGAACAAATAGAGGAGA
AGCTTCTCACGAAGCCAAGG

Product: translation initiation factor IF-2 subunit gamma

Products: NA

Alternate protein names: aIF2-gamma; eIF-2-gamma [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MPWDIKPPEVNIGVVGHVDHGKTTLVQALTGVWTAKHSLEIERGMTIKLGYADGNIAYCDGLPLPDAYTIKEYCPDGSES
KLLRRVSYVDAPGHELYMTTMLSGAMIMDGAILVIAANEPCPQPQTVEHLMALNILGLRNIIIVQNKIDVVDKKRALENY
NEILKLVKGTVAENAPIIPVSALHHINIDALLQAIQEIIPTPERDYTKPPLMYVVRSFDVNKPGTPYDKIEGGVIGGSLL
QGRFRVGDEIMILPGIKTQVGSAKSQVSKYEPIVTTIEEVRYGNLIVDEARPGGLVAIQTKLDPSLTKGDQLVGSIVTTP
RNNIPVVKTLEVRFQLFERIVGTKELGKLPPIQVNEKIAVAIGTANRVAVVKSIRKDIMTIELTDPVATWNGSRIAISRR
VLARWRLAGWGIIEGVSE

Sequences:

>Translated_418_residues
MPWDIKPPEVNIGVVGHVDHGKTTLVQALTGVWTAKHSLEIERGMTIKLGYADGNIAYCDGLPLPDAYTIKEYCPDGSES
KLLRRVSYVDAPGHELYMTTMLSGAMIMDGAILVIAANEPCPQPQTVEHLMALNILGLRNIIIVQNKIDVVDKKRALENY
NEILKLVKGTVAENAPIIPVSALHHINIDALLQAIQEIIPTPERDYTKPPLMYVVRSFDVNKPGTPYDKIEGGVIGGSLL
QGRFRVGDEIMILPGIKTQVGSAKSQVSKYEPIVTTIEEVRYGNLIVDEARPGGLVAIQTKLDPSLTKGDQLVGSIVTTP
RNNIPVVKTLEVRFQLFERIVGTKELGKLPPIQVNEKIAVAIGTANRVAVVKSIRKDIMTIELTDPVATWNGSRIAISRR
VLARWRLAGWGIIEGVSE
>Mature_417_residues
PWDIKPPEVNIGVVGHVDHGKTTLVQALTGVWTAKHSLEIERGMTIKLGYADGNIAYCDGLPLPDAYTIKEYCPDGSESK
LLRRVSYVDAPGHELYMTTMLSGAMIMDGAILVIAANEPCPQPQTVEHLMALNILGLRNIIIVQNKIDVVDKKRALENYN
EILKLVKGTVAENAPIIPVSALHHINIDALLQAIQEIIPTPERDYTKPPLMYVVRSFDVNKPGTPYDKIEGGVIGGSLLQ
GRFRVGDEIMILPGIKTQVGSAKSQVSKYEPIVTTIEEVRYGNLIVDEARPGGLVAIQTKLDPSLTKGDQLVGSIVTTPR
NNIPVVKTLEVRFQLFERIVGTKELGKLPPIQVNEKIAVAIGTANRVAVVKSIRKDIMTIELTDPVATWNGSRIAISRRV
LARWRLAGWGIIEGVSE

Specific function: eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA [H]

COG id: COG5257

COG function: function code J; Translation initiation factor 2, gamma subunit (eIF-2gamma; GTPase)

Gene ontology:

Cell location: Cytoplasmic, and membrane-associated [C]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EIF2G subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503507, Length=421, Percent_Identity=43.4679334916865, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI34147630, Length=259, Percent_Identity=32.8185328185328, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI54607086, Length=300, Percent_Identity=29.3333333333333, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1789737, Length=293, Percent_Identity=31.740614334471, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1790412, Length=293, Percent_Identity=31.740614334471, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI2367247, Length=243, Percent_Identity=31.2757201646091, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17509919, Length=421, Percent_Identity=42.0427553444181, Blast_Score=342, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI17556456, Length=301, Percent_Identity=30.2325581395349, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI25141371, Length=296, Percent_Identity=30.4054054054054, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17506081, Length=254, Percent_Identity=27.5590551181102, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320863, Length=422, Percent_Identity=43.3649289099526, Blast_Score=341, Evalue=2e-94,
Organism=Saccharomyces cerevisiae, GI6324761, Length=264, Percent_Identity=37.1212121212121, Blast_Score=132, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24647046, Length=423, Percent_Identity=42.789598108747, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI24647048, Length=423, Percent_Identity=42.789598108747, Blast_Score=339, Evalue=3e-93,
Organism=Drosophila melanogaster, GI19921738, Length=238, Percent_Identity=34.0336134453782, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI19922690, Length=262, Percent_Identity=33.969465648855, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI281363316, Length=294, Percent_Identity=30.6122448979592, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI17864358, Length=294, Percent_Identity=30.6122448979592, Blast_Score=105, Evalue=6e-23,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR022424
- InterPro:   IPR015256
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF09173 eIF2_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 45775; Mature: 45644

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPWDIKPPEVNIGVVGHVDHGKTTLVQALTGVWTAKHSLEIERGMTIKLGYADGNIAYCD
CCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCEEECCCCEEEEEECCCCEEEEC
GLPLPDAYTIKEYCPDGSESKLLRRVSYVDAPGHELYMTTMLSGAMIMDGAILVIAANEP
CCCCCCCEEHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEHHHCCCEEECCEEEEEECCCC
CPQPQTVEHLMALNILGLRNIIIVQNKIDVVDKKRALENYNEILKLVKGTVAENAPIIPV
CCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
SALHHINIDALLQAIQEIIPTPERDYTKPPLMYVVRSFDVNKPGTPYDKIEGGVIGGSLL
HHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHEECCCCCCCCCCHHHCCCCEECHHHH
QGRFRVGDEIMILPGIKTQVGSAKSQVSKYEPIVTTIEEVRYGNLIVDEARPGGLVAIQT
CCCEECCCEEEEECCCCCHHCCHHHHHHHCCHHHHHHHHHCCCCEEEECCCCCCEEEEEE
KLDPSLTKGDQLVGSIVTTPRNNIPVVKTLEVRFQLFERIVGTKELGKLPPIQVNEKIAV
CCCCCCCCCHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHCCHHHCCCCCCEECCEEEE
AIGTANRVAVVKSIRKDIMTIELTDPVATWNGSRIAISRRVLARWRLAGWGIIEGVSE
EEECCCHHHHHHHHHHCEEEEEECCCEECCCCCEEEEHHHHHHHHHHCCCHHHCCCCC
>Mature Secondary Structure 
PWDIKPPEVNIGVVGHVDHGKTTLVQALTGVWTAKHSLEIERGMTIKLGYADGNIAYCD
CCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCEEECCCCEEEEEECCCCEEEEC
GLPLPDAYTIKEYCPDGSESKLLRRVSYVDAPGHELYMTTMLSGAMIMDGAILVIAANEP
CCCCCCCEEHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEHHHCCCEEECCEEEEEECCCC
CPQPQTVEHLMALNILGLRNIIIVQNKIDVVDKKRALENYNEILKLVKGTVAENAPIIPV
CCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
SALHHINIDALLQAIQEIIPTPERDYTKPPLMYVVRSFDVNKPGTPYDKIEGGVIGGSLL
HHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHEECCCCCCCCCCHHHCCCCEECHHHH
QGRFRVGDEIMILPGIKTQVGSAKSQVSKYEPIVTTIEEVRYGNLIVDEARPGGLVAIQT
CCCEECCCEEEEECCCCCHHCCHHHHHHHCCHHHHHHHHHCCCCEEEECCCCCCEEEEEE
KLDPSLTKGDQLVGSIVTTPRNNIPVVKTLEVRFQLFERIVGTKELGKLPPIQVNEKIAV
CCCCCCCCCHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHCCHHHCCCCCCEECCEEEE
AIGTANRVAVVKSIRKDIMTIELTDPVATWNGSRIAISRRVLARWRLAGWGIIEGVSE
EEECCCHHHHHHHHHHCEEEEEECCCEECCCCCEEEEHHHHHHHHHHCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]