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The map label for this gene is pol-alpha [H]

Identifier: 218884625

GI number: 218884625

Start: 1226731

End: 1229301

Strand: Reverse

Name: pol-alpha [H]

Synonym: DKAM_1314

Alternate gene names: 218884625

Gene position: 1229301-1226731 (Counterclockwise)

Preceding gene: 218884626

Following gene: 218884624

Centisome position: 90.04

GC content: 42.2

Gene sequence:

>2571_bases
ATGGATACACCACTACATGTTAAGGAGTCTTTCACATCGTACAAGGTTTTAAGTAAACCACACTACCTGCTCGGAGTATA
CTATGATGGAAGGCTGGGGAAAGCAATACTAGAGTTCCTCGATGAGGATGGCAGTAAACTGGTAAAAATAATGGATCCAG
TTGGGCATAAGCCATATTTCCTAACAGATCTAAGCATTGACGAGGTAAAATCATTAAATGATATAGCCAGGGATAAGCAT
GTCGAAGGCTTTGAGGAAGTATATAAGACTAACCCGTTAACCATGAAGAAGATCAGAGTCACCAAGATAATCACGAGAGA
CCCATTAGCAGTGAAGAAGCTAAGGTCAAAGATGCCTAGTAACGCCTCTGTATGGGAAGCTAAAATCAAATACCATAATA
ACTATGTCTATGATAATGGATTAATCCCGGGAATGAAGTACATCATCGAGAACGATGGCTCCAAGCCGAGAATAACACTG
GTGAAACCCGAGATACCGTTAACACTGATACAATCGTTAAGGGAATTATTTAAGAACGAGCCCGAGGAAACTATTAAACT
AGCCGAGGACTACCTACTACTATTTGAGGAAGCGCCACCAAAAGCATTGAGAATAGCTATCGATATCGAGGTGTATACGC
CCTTTAAAGGTAGAATACCTAATCCAAGACAGGGAGAATACCCTGTTATCAGTATAGGGCTTTCATCAAATAATGGCTTT
AGAAAGGTACTTCTACTGGGGAGAAGATTCAAAGAGAGATTCGACTACATGTCTGATGACTACCCTGTCGATATTGAGGT
TGAGGTATTCGACTCAGAGAAGGCGTTGATCCTTGAAGCTCTCAAGACTATTTCAAGGTACCCTGTAGTACTCACTTATA
ATGGGGATAACTTCGACTTAATGTATATTTATACAAGGGCCATAAGACTAGGGATACCGAGACACACAATCCCATTCATC
CTCGGCGAGGACATGGTTAGAATTGATACAGGGATACACCTAGACCTCTATAAGTTCTTCTCGAATAGAGCCGTGAAGAA
CTATGCCTTTGGAGGCAAGTACCAGGAGGAGAAGCTTGATGCGGTGTCCTCGGCACTCCTCGGCATATCTAAAATAGGGT
TTGAGGAGGCGATCGGCGATATCTCGGCATCCCACCTTATAGCTTACAACATGAGGGACGCCGATATAACTCTACAGTTA
ACTATATTCAGCAACGAGCTTGTATGGAAGCTAATGATTCTACTAGCCCGTATTTCCAAAACGAGTATCGAGGATGTGTG
TAGACGACAGATATCTAACTGGATACAGAACCAGTTCTTCTGGGAGCATAGGAGGCTGGGATATCTTATACCTAATAGTG
AGGATATAAGAAGGTATGCTGGTAAAGCAGGTACAAAAGCGACTATCGAAGGTAAGAAATACGCTGGTGCACTGGTAATT
GAGCCTCCACGAGGCATATTCTTCAATGTAACAGTACTAGATATAGCGTCACTGTACCCAAGCATAATTAAAAAATATAA
TTTAAGCTATGAAACGGTTGATATGCCCTGGTGTAAGTCAACAATAGATATAATTGATGAAACGGGTAGAAAACTACACG
TGGTCTGCGTTGAGAAACCTGGTTTAACAGCACAGATCACGGGTATACTGAGGGATTTTAGAGTTGGAATATACAAGAAG
AGAGCAAAGGATAAGACATTACCTAAAGACCTACTGGCATGGTATGACGTTGTCCAACGCGCTATAAAAGTCTTCATTAA
CGCGAGCTATGGCGTCTTCGGTGATGAACGATTCTCCTTATTCTCGCCAGCTGTGGCTGAAAGCGTTACAGCCATGGGTA
GGAAGTCTTTTCTAACAATTATTCTCAAAGCTGCCGAACTAGGGATAAAGCCATTATATGGCGACACAGACTCTATCTTC
GTGTGGAATCCTAACCAAAGCCAGCTGGAGATGCTCCAGAAATGGATACTGGAAAACCTCGGCTTGGAAATAGAGTTGGA
TAAGGAGTTCACATACGTGGTTTTCACAGGGTTGAAAAAGAATTACCTAGGCAGGATGAAGGACGGGGGGATCGAGATAA
AGGGGCTCGTCGCAAAGAAGCGGAACACACCAGAGTTTCTTAAGGAGCTATTTAAGGAGATGCTAGAGAAGATGAAGGCT
ATTGAAACCCCTGAGAACTTCGTGGTGTTCGTTGACTGGCTTAGAGAGATGCTGAAAGAATATTATGATGGATTAAAGCG
GAAGGAGATACCATTGGATCATCTAGCGATAAGGGTTGCATTAACCAAGCCTCCTTCAAGTTATAATAAAACGAAGCCAC
CGCATGTAAAGGCAGCCCAGCAATTAATCGATTATGGTATCAGCGTGCAGGAAGGCGATATAATAACCTATGTTAAAGTA
AAGAGCAGGGACGATTATAAGGCAATACAGTTAACCAGGCTTCATGAAGTAGATCCTGAGAAATATATTGAATTATTGAA
GAGCGGGTTAGAGCAATTATTATCCGCTCTCTCCATAAAATGGGAGGACATAGTTGGAGTAAAGCTAGCAAGCTATATGT
CACAAAGATAA

Upstream 100 bases:

>100_bases
GCTAGTTACGAGAAAGCCAGGGAAGCTCTATTAAAGACAAGGGGAGATATTCTGCAGGCAATAATGATTCTCAGAGAAGG
ATGAAGGATCCATGCGAAGC

Downstream 100 bases:

>100_bases
ATGGTGTTCGATATTGGAATCCCGCATTAAATTAATGTTGGCTGTAACCATTATTCTAGTGATTTTATCGATAATACTAC
ATATGCCCATAGAAGTCCAG

Product: DNA polymerase I

Products: NA

Alternate protein names: DNA polymerase B1; DNA polymerase I [H]

Number of amino acids: Translated: 856; Mature: 856

Protein sequence:

>856_residues
MDTPLHVKESFTSYKVLSKPHYLLGVYYDGRLGKAILEFLDEDGSKLVKIMDPVGHKPYFLTDLSIDEVKSLNDIARDKH
VEGFEEVYKTNPLTMKKIRVTKIITRDPLAVKKLRSKMPSNASVWEAKIKYHNNYVYDNGLIPGMKYIIENDGSKPRITL
VKPEIPLTLIQSLRELFKNEPEETIKLAEDYLLLFEEAPPKALRIAIDIEVYTPFKGRIPNPRQGEYPVISIGLSSNNGF
RKVLLLGRRFKERFDYMSDDYPVDIEVEVFDSEKALILEALKTISRYPVVLTYNGDNFDLMYIYTRAIRLGIPRHTIPFI
LGEDMVRIDTGIHLDLYKFFSNRAVKNYAFGGKYQEEKLDAVSSALLGISKIGFEEAIGDISASHLIAYNMRDADITLQL
TIFSNELVWKLMILLARISKTSIEDVCRRQISNWIQNQFFWEHRRLGYLIPNSEDIRRYAGKAGTKATIEGKKYAGALVI
EPPRGIFFNVTVLDIASLYPSIIKKYNLSYETVDMPWCKSTIDIIDETGRKLHVVCVEKPGLTAQITGILRDFRVGIYKK
RAKDKTLPKDLLAWYDVVQRAIKVFINASYGVFGDERFSLFSPAVAESVTAMGRKSFLTIILKAAELGIKPLYGDTDSIF
VWNPNQSQLEMLQKWILENLGLEIELDKEFTYVVFTGLKKNYLGRMKDGGIEIKGLVAKKRNTPEFLKELFKEMLEKMKA
IETPENFVVFVDWLREMLKEYYDGLKRKEIPLDHLAIRVALTKPPSSYNKTKPPHVKAAQQLIDYGISVQEGDIITYVKV
KSRDDYKAIQLTRLHEVDPEKYIELLKSGLEQLLSALSIKWEDIVGVKLASYMSQR

Sequences:

>Translated_856_residues
MDTPLHVKESFTSYKVLSKPHYLLGVYYDGRLGKAILEFLDEDGSKLVKIMDPVGHKPYFLTDLSIDEVKSLNDIARDKH
VEGFEEVYKTNPLTMKKIRVTKIITRDPLAVKKLRSKMPSNASVWEAKIKYHNNYVYDNGLIPGMKYIIENDGSKPRITL
VKPEIPLTLIQSLRELFKNEPEETIKLAEDYLLLFEEAPPKALRIAIDIEVYTPFKGRIPNPRQGEYPVISIGLSSNNGF
RKVLLLGRRFKERFDYMSDDYPVDIEVEVFDSEKALILEALKTISRYPVVLTYNGDNFDLMYIYTRAIRLGIPRHTIPFI
LGEDMVRIDTGIHLDLYKFFSNRAVKNYAFGGKYQEEKLDAVSSALLGISKIGFEEAIGDISASHLIAYNMRDADITLQL
TIFSNELVWKLMILLARISKTSIEDVCRRQISNWIQNQFFWEHRRLGYLIPNSEDIRRYAGKAGTKATIEGKKYAGALVI
EPPRGIFFNVTVLDIASLYPSIIKKYNLSYETVDMPWCKSTIDIIDETGRKLHVVCVEKPGLTAQITGILRDFRVGIYKK
RAKDKTLPKDLLAWYDVVQRAIKVFINASYGVFGDERFSLFSPAVAESVTAMGRKSFLTIILKAAELGIKPLYGDTDSIF
VWNPNQSQLEMLQKWILENLGLEIELDKEFTYVVFTGLKKNYLGRMKDGGIEIKGLVAKKRNTPEFLKELFKEMLEKMKA
IETPENFVVFVDWLREMLKEYYDGLKRKEIPLDHLAIRVALTKPPSSYNKTKPPHVKAAQQLIDYGISVQEGDIITYVKV
KSRDDYKAIQLTRLHEVDPEKYIELLKSGLEQLLSALSIKWEDIVGVKLASYMSQR
>Mature_856_residues
MDTPLHVKESFTSYKVLSKPHYLLGVYYDGRLGKAILEFLDEDGSKLVKIMDPVGHKPYFLTDLSIDEVKSLNDIARDKH
VEGFEEVYKTNPLTMKKIRVTKIITRDPLAVKKLRSKMPSNASVWEAKIKYHNNYVYDNGLIPGMKYIIENDGSKPRITL
VKPEIPLTLIQSLRELFKNEPEETIKLAEDYLLLFEEAPPKALRIAIDIEVYTPFKGRIPNPRQGEYPVISIGLSSNNGF
RKVLLLGRRFKERFDYMSDDYPVDIEVEVFDSEKALILEALKTISRYPVVLTYNGDNFDLMYIYTRAIRLGIPRHTIPFI
LGEDMVRIDTGIHLDLYKFFSNRAVKNYAFGGKYQEEKLDAVSSALLGISKIGFEEAIGDISASHLIAYNMRDADITLQL
TIFSNELVWKLMILLARISKTSIEDVCRRQISNWIQNQFFWEHRRLGYLIPNSEDIRRYAGKAGTKATIEGKKYAGALVI
EPPRGIFFNVTVLDIASLYPSIIKKYNLSYETVDMPWCKSTIDIIDETGRKLHVVCVEKPGLTAQITGILRDFRVGIYKK
RAKDKTLPKDLLAWYDVVQRAIKVFINASYGVFGDERFSLFSPAVAESVTAMGRKSFLTIILKAAELGIKPLYGDTDSIF
VWNPNQSQLEMLQKWILENLGLEIELDKEFTYVVFTGLKKNYLGRMKDGGIEIKGLVAKKRNTPEFLKELFKEMLEKMKA
IETPENFVVFVDWLREMLKEYYDGLKRKEIPLDHLAIRVALTKPPSSYNKTKPPHVKAAQQLIDYGISVQEGDIITYVKV
KSRDDYKAIQLTRLHEVDPEKYIELLKSGLEQLLSALSIKWEDIVGVKLASYMSQR

Specific function: Thought To Be Involved In DNA Repair And/Or Mutagenesis. [C]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-B family [H]

Homologues:

Organism=Homo sapiens, GI106507301, Length=475, Percent_Identity=25.6842105263158, Blast_Score=103, Evalue=9e-22,
Organism=Homo sapiens, GI156616275, Length=607, Percent_Identity=26.1943986820428, Blast_Score=98, Evalue=4e-20,
Organism=Escherichia coli, GI1786246, Length=772, Percent_Identity=22.9274611398964, Blast_Score=93, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI32565317, Length=358, Percent_Identity=28.2122905027933, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559488, Length=587, Percent_Identity=26.2350936967632, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324227, Length=469, Percent_Identity=26.226012793177, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320101, Length=577, Percent_Identity=24.6100519930676, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648780, Length=594, Percent_Identity=23.7373737373737, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24664937, Length=617, Percent_Identity=24.7974068071313, Blast_Score=84, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR017964
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR023211
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 98223; Mature: 98223

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS00116 DNA_POLYMERASE_B

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTPLHVKESFTSYKVLSKPHYLLGVYYDGRLGKAILEFLDEDGSKLVKIMDPVGHKPYF
CCCCCCHHHCCHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCCHHHHHHHCCCCCCCEE
LTDLSIDEVKSLNDIARDKHVEGFEEVYKTNPLTMKKIRVTKIITRDPLAVKKLRSKMPS
EECCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEEEEEECCCCHHHHHHHHHCCC
NASVWEAKIKYHNNYVYDNGLIPGMKYIIENDGSKPRITLVKPEIPLTLIQSLRELFKNE
CCCEEEEEEEEECCEEECCCCCCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCC
PEETIKLAEDYLLLFEEAPPKALRIAIDIEVYTPFKGRIPNPRQGEYPVISIGLSSNNGF
CHHHHHHHHHHEEEEECCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCH
RKVLLLGRRFKERFDYMSDDYPVDIEVEVFDSEKALILEALKTISRYPVVLTYNGDNFDL
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCEEE
MYIYTRAIRLGIPRHTIPFILGEDMVRIDTGIHLDLYKFFSNRAVKNYAFGGKYQEEKLD
EEEEEHHHHHCCCCCCCCCEECCCEEEECCCCCHHHHHHHCCCCHHHCCCCCCCHHHHHH
AVSSALLGISKIGFEEAIGDISASHLIAYNMRDADITLQLTIFSNELVWKLMILLARISK
HHHHHHHHHHHCCHHHHHCCCCCCEEEEEECCCCCEEEEEEEECCHHHHHHHHHHHHHCC
TSIEDVCRRQISNWIQNQFFWEHRRLGYLIPNSEDIRRYAGKAGTKATIEGKKYAGALVI
CCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHCCCCCEEEECCCCCCCEEEE
EPPRGIFFNVTVLDIASLYPSIIKKYNLSYETVDMPWCKSTIDIIDETGRKLHVVCVEKP
ECCCCEEEEEHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCCEEEEEEEECC
GLTAQITGILRDFRVGIYKKRAKDKTLPKDLLAWYDVVQRAIKVFINASYGVFGDERFSL
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
FSPAVAESVTAMGRKSFLTIILKAAELGIKPLYGDTDSIFVWNPNQSQLEMLQKWILENL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHC
GLEIELDKEFTYVVFTGLKKNYLGRMKDGGIEIKGLVAKKRNTPEFLKELFKEMLEKMKA
CCEEEECCCEEEEEEECCHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
IETPENFVVFVDWLREMLKEYYDGLKRKEIPLDHLAIRVALTKPPSSYNKTKPPHVKAAQ
HCCCCHHEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHH
QLIDYGISVQEGDIITYVKVKSRDDYKAIQLTRLHEVDPEKYIELLKSGLEQLLSALSIK
HHHHCCCEEECCCEEEEEEEECCCCCCCEEEEHHHCCCHHHHHHHHHHHHHHHHHHHCCC
WEDIVGVKLASYMSQR
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDTPLHVKESFTSYKVLSKPHYLLGVYYDGRLGKAILEFLDEDGSKLVKIMDPVGHKPYF
CCCCCCHHHCCHHHHHHCCCCEEEEEEECCHHHHHHHHHHCCCCHHHHHHHCCCCCCCEE
LTDLSIDEVKSLNDIARDKHVEGFEEVYKTNPLTMKKIRVTKIITRDPLAVKKLRSKMPS
EECCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEEEEEECCCCHHHHHHHHHCCC
NASVWEAKIKYHNNYVYDNGLIPGMKYIIENDGSKPRITLVKPEIPLTLIQSLRELFKNE
CCCEEEEEEEEECCEEECCCCCCCCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCC
PEETIKLAEDYLLLFEEAPPKALRIAIDIEVYTPFKGRIPNPRQGEYPVISIGLSSNNGF
CHHHHHHHHHHEEEEECCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCH
RKVLLLGRRFKERFDYMSDDYPVDIEVEVFDSEKALILEALKTISRYPVVLTYNGDNFDL
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCEEE
MYIYTRAIRLGIPRHTIPFILGEDMVRIDTGIHLDLYKFFSNRAVKNYAFGGKYQEEKLD
EEEEEHHHHHCCCCCCCCCEECCCEEEECCCCCHHHHHHHCCCCHHHCCCCCCCHHHHHH
AVSSALLGISKIGFEEAIGDISASHLIAYNMRDADITLQLTIFSNELVWKLMILLARISK
HHHHHHHHHHHCCHHHHHCCCCCCEEEEEECCCCCEEEEEEEECCHHHHHHHHHHHHHCC
TSIEDVCRRQISNWIQNQFFWEHRRLGYLIPNSEDIRRYAGKAGTKATIEGKKYAGALVI
CCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHCCCCCEEEECCCCCCCEEEE
EPPRGIFFNVTVLDIASLYPSIIKKYNLSYETVDMPWCKSTIDIIDETGRKLHVVCVEKP
ECCCCEEEEEHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCCEEEEEEEECC
GLTAQITGILRDFRVGIYKKRAKDKTLPKDLLAWYDVVQRAIKVFINASYGVFGDERFSL
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
FSPAVAESVTAMGRKSFLTIILKAAELGIKPLYGDTDSIFVWNPNQSQLEMLQKWILENL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHC
GLEIELDKEFTYVVFTGLKKNYLGRMKDGGIEIKGLVAKKRNTPEFLKELFKEMLEKMKA
CCEEEECCCEEEEEEECCHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
IETPENFVVFVDWLREMLKEYYDGLKRKEIPLDHLAIRVALTKPPSSYNKTKPPHVKAAQ
HCCCCHHEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHH
QLIDYGISVQEGDIITYVKVKSRDDYKAIQLTRLHEVDPEKYIELLKSGLEQLLSALSIK
HHHHCCCEEECCCEEEEEEEECCCCCCCEEEEHHHCCCHHHHHHHHHHHHHHHHHHHCCC
WEDIVGVKLASYMSQR
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10997874 [H]