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The map label for this gene is polB [H]

Identifier: 218884606

GI number: 218884606

Start: 1211801

End: 1214194

Strand: Reverse

Name: polB [H]

Synonym: DKAM_1295

Alternate gene names: 218884606

Gene position: 1214194-1211801 (Counterclockwise)

Preceding gene: 218884608

Following gene: 218884605

Centisome position: 88.94

GC content: 46.03

Gene sequence:

>2394_bases
ATGGAGATCGAATTTTACCTTTTAGACGTAACTTATGAGGTTGTTGGCAAGGAACCCCATGTAATCCTATGGGGTATCAC
ACGTAATGGTGAACGAGTAGTACTTAGAGACAGGAGGTTCCGTCCATATTTTTACGCCATTATAAATGAGAGGATAGATG
CTGAAACCGTAGCCAGGAAGATAAGAGCCTTCAGCGAGGCTGAGTCGCCTATAATCGAGGTGAAGCCGACTGAGAAGAAG
TACTTCGGGAAACCAGTTAAAGCATTAAGGATCACAACCGTGATCCCAGAGTATGTTAGAAAGTATAGGGAGAGGATAAG
GGAGATTAGTGAGGTAGTAGATGTACTAGAGGCTGATATAAGGTTCAGCATGAGGTATCTCCTAGACCACGGGTTAGAGC
CATGTGGATGGCATATTGCAGAAGTGATGGAGGCCCCTGTTCCAGCTAAATACAGGGCCACACGCTTCTACGAGGTCGTA
AGCTCCATTAAACCCATAGGGGATACTAGGCCGCCTAAGGATCTACGGGTAATGGCGTTCGATATAGAGGTATACAGTGA
AACCGGTACACCCCATCCGGAGAGAGACCCCGTTATAATAATAGGGGGGAAAACAAGCAGCGGCGAGGTCAAGCAGTTTA
TAGCTGAAGAATTAAGCGATAAAGATGTCATCGCGGAATTCACAGACTATATCCTCAGGTACGACCCCGATATAATAGTG
GGGTATAACAGTAGCAGGTTCGACTGGCCCTACCTAATGGAGCGTGCACGAGTAAACAACTTAAAGCTTGATGTAGGCCG
TAGACGAGATGGACAGCCCTCTCAAAGCACTTATGGACACATATCAATACCTGGAAGAATCCATGTGGATCTCTTGAACT
TCGCTGAAGAAATACCAGAGGTGAAGTTAAAGAGCCTGGACATCGTGGCTGACTATCTAGGTGTAATGAAGAGAAGTGAG
AGAGTACTCATCGACTACATAGAGTTCCCAAGGTACTGGAGAGATCCGGCAAGAAAACCCTTGTTGTTGCGATATAATTT
AGATGATGTAGAGTCAACAATGGGTCTGGCAGAGAAGTTCCTGCCTTTTGCAATGCAGTTGTCGAGTATAACAGGGCTTC
CCCTGGATCAAGTTGGAGCGGCCAGCGTGGGGAATAGGCTTGAATGGTTTTTAATGAGGGAGGCCTTCCGCAGGGATGAA
TTGATCCCGAATAGAATCGAGAAAGAGGTCGAGCCCTACAAGGGGGCGGTGGTGCTGCAGCCATTACCTGGTATACACGA
GAACATTGCTGTACTGGATTTCACAAGCATGTACCCTAACATTATGATAAAGTACAATATTGGGCCAGACACCATCATAA
GGGAGGGTGTATGCCGTGAGAGTGAACACTATGTAGCACCCGAGGTTAAGCACTGCTTCCTGAGGGAGCCACCTGGATTC
TTCAAAGGCGTACTTCAGATACTGCTCTCACTGAGGAAACAGATAAGGGGGGAGATGAAGAAGTATAAGACGGATTCACC
GGAGTATAGATTGCTTGATGAGAGACAGAAGGCGATCAAGGTATTAGCTAACGCGACATACGGGTACATGGGGTGGGTAG
CAGCTAGATGGTATTGCAAGGAGTGCGCGGAAGCTGTTACAGCATGGGGCCGTAGAACAATATTAAGTGTCATAGATTAC
GCTAGGGTAATAGGGCTTAAAGTAATATATGGCGACACCGACTCCCTCTTTGTTGAAAACATACCTGATAAAATAACCAG
GCTCTCAGAGTACGTTGAACGCGAGCTGGGCTTCGAGATAAAAATAGACAAGGTGTATAAGAGGGTCTTCTTCACAGAGG
CAAAGAAACGGTATGCCGGGCTACTTGAAGATGGACGAGTAGACATAGTTGGATTCGAGGCTGTGAGAGGAGACTGGGCT
GAGATAGCTAAGGAGGTACAGGAGAAGGTAACCGAGATCCTATTAAGCGAGAAAAACATCGAGAAGGCAATAGAATACGT
GAGAAACATCATAGCGGAGTTGAACCAGGGTAGAATACCCCTTGAAAAACTAATTATATGGAAGACCCTTACGAAAAGCG
TTGAGGAATACGAGGCGGAAGCCCCCCATGTGAATGCAGCTAGAAAAATGAAGAGGATGGGTATGAAGATAGAGGTCGGT
GATAAAATAGGATATATTGTTGTAAAGGGCTCCGGCAAGATATCCGATAGAGCCGAACCCTATATGTTCGTGAAGGACCC
CAAGCTAGTGGACACTATATATTATATTGATCACCAAATAATACCTGCCGCACTAAGGATCCTAGAGTATTTTGGAGTAA
CCGATACACAATTGAAAAAAGTAGCCAGCACAGCTGGCAGGAAAAGCCTGTTCGACTACACCAAGAAGACATAA

Upstream 100 bases:

>100_bases
TCAACTACACCTTCAGCTGACACAGCATCTGCATGCTTTCTCTATAGTAATAGCTTTATGAGCGTATTAAGATATCATAT
TATTGAAGCGTGAAAAAACA

Downstream 100 bases:

>100_bases
CCTCTTCCCGCCCTTGAGGGCGAGGGCTTCCAGTTGTAAGTGTAGATTTATAAGGTTATGCTTGATTAGGTCATTAAGTA
GATCCAGTAATAATTGGTAT

Product: DNA polymerase II

Products: NA

Alternate protein names: DNA polymerase II [H]

Number of amino acids: Translated: 797; Mature: 797

Protein sequence:

>797_residues
MEIEFYLLDVTYEVVGKEPHVILWGITRNGERVVLRDRRFRPYFYAIINERIDAETVARKIRAFSEAESPIIEVKPTEKK
YFGKPVKALRITTVIPEYVRKYRERIREISEVVDVLEADIRFSMRYLLDHGLEPCGWHIAEVMEAPVPAKYRATRFYEVV
SSIKPIGDTRPPKDLRVMAFDIEVYSETGTPHPERDPVIIIGGKTSSGEVKQFIAEELSDKDVIAEFTDYILRYDPDIIV
GYNSSRFDWPYLMERARVNNLKLDVGRRRDGQPSQSTYGHISIPGRIHVDLLNFAEEIPEVKLKSLDIVADYLGVMKRSE
RVLIDYIEFPRYWRDPARKPLLLRYNLDDVESTMGLAEKFLPFAMQLSSITGLPLDQVGAASVGNRLEWFLMREAFRRDE
LIPNRIEKEVEPYKGAVVLQPLPGIHENIAVLDFTSMYPNIMIKYNIGPDTIIREGVCRESEHYVAPEVKHCFLREPPGF
FKGVLQILLSLRKQIRGEMKKYKTDSPEYRLLDERQKAIKVLANATYGYMGWVAARWYCKECAEAVTAWGRRTILSVIDY
ARVIGLKVIYGDTDSLFVENIPDKITRLSEYVERELGFEIKIDKVYKRVFFTEAKKRYAGLLEDGRVDIVGFEAVRGDWA
EIAKEVQEKVTEILLSEKNIEKAIEYVRNIIAELNQGRIPLEKLIIWKTLTKSVEEYEAEAPHVNAARKMKRMGMKIEVG
DKIGYIVVKGSGKISDRAEPYMFVKDPKLVDTIYYIDHQIIPAALRILEYFGVTDTQLKKVASTAGRKSLFDYTKKT

Sequences:

>Translated_797_residues
MEIEFYLLDVTYEVVGKEPHVILWGITRNGERVVLRDRRFRPYFYAIINERIDAETVARKIRAFSEAESPIIEVKPTEKK
YFGKPVKALRITTVIPEYVRKYRERIREISEVVDVLEADIRFSMRYLLDHGLEPCGWHIAEVMEAPVPAKYRATRFYEVV
SSIKPIGDTRPPKDLRVMAFDIEVYSETGTPHPERDPVIIIGGKTSSGEVKQFIAEELSDKDVIAEFTDYILRYDPDIIV
GYNSSRFDWPYLMERARVNNLKLDVGRRRDGQPSQSTYGHISIPGRIHVDLLNFAEEIPEVKLKSLDIVADYLGVMKRSE
RVLIDYIEFPRYWRDPARKPLLLRYNLDDVESTMGLAEKFLPFAMQLSSITGLPLDQVGAASVGNRLEWFLMREAFRRDE
LIPNRIEKEVEPYKGAVVLQPLPGIHENIAVLDFTSMYPNIMIKYNIGPDTIIREGVCRESEHYVAPEVKHCFLREPPGF
FKGVLQILLSLRKQIRGEMKKYKTDSPEYRLLDERQKAIKVLANATYGYMGWVAARWYCKECAEAVTAWGRRTILSVIDY
ARVIGLKVIYGDTDSLFVENIPDKITRLSEYVERELGFEIKIDKVYKRVFFTEAKKRYAGLLEDGRVDIVGFEAVRGDWA
EIAKEVQEKVTEILLSEKNIEKAIEYVRNIIAELNQGRIPLEKLIIWKTLTKSVEEYEAEAPHVNAARKMKRMGMKIEVG
DKIGYIVVKGSGKISDRAEPYMFVKDPKLVDTIYYIDHQIIPAALRILEYFGVTDTQLKKVASTAGRKSLFDYTKKT
>Mature_797_residues
MEIEFYLLDVTYEVVGKEPHVILWGITRNGERVVLRDRRFRPYFYAIINERIDAETVARKIRAFSEAESPIIEVKPTEKK
YFGKPVKALRITTVIPEYVRKYRERIREISEVVDVLEADIRFSMRYLLDHGLEPCGWHIAEVMEAPVPAKYRATRFYEVV
SSIKPIGDTRPPKDLRVMAFDIEVYSETGTPHPERDPVIIIGGKTSSGEVKQFIAEELSDKDVIAEFTDYILRYDPDIIV
GYNSSRFDWPYLMERARVNNLKLDVGRRRDGQPSQSTYGHISIPGRIHVDLLNFAEEIPEVKLKSLDIVADYLGVMKRSE
RVLIDYIEFPRYWRDPARKPLLLRYNLDDVESTMGLAEKFLPFAMQLSSITGLPLDQVGAASVGNRLEWFLMREAFRRDE
LIPNRIEKEVEPYKGAVVLQPLPGIHENIAVLDFTSMYPNIMIKYNIGPDTIIREGVCRESEHYVAPEVKHCFLREPPGF
FKGVLQILLSLRKQIRGEMKKYKTDSPEYRLLDERQKAIKVLANATYGYMGWVAARWYCKECAEAVTAWGRRTILSVIDY
ARVIGLKVIYGDTDSLFVENIPDKITRLSEYVERELGFEIKIDKVYKRVFFTEAKKRYAGLLEDGRVDIVGFEAVRGDWA
EIAKEVQEKVTEILLSEKNIEKAIEYVRNIIAELNQGRIPLEKLIIWKTLTKSVEEYEAEAPHVNAARKMKRMGMKIEVG
DKIGYIVVKGSGKISDRAEPYMFVKDPKLVDTIYYIDHQIIPAALRILEYFGVTDTQLKKVASTAGRKSLFDYTKKT

Specific function: Thought To Be Involved In DNA Repair And/Or Mutagenesis. [C]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-B family [H]

Homologues:

Organism=Homo sapiens, GI156616275, Length=861, Percent_Identity=27.5261324041812, Blast_Score=258, Evalue=1e-68,
Organism=Homo sapiens, GI106507301, Length=622, Percent_Identity=25.8842443729904, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI153792012, Length=705, Percent_Identity=25.531914893617, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI62198237, Length=334, Percent_Identity=22.7544910179641, Blast_Score=68, Evalue=4e-11,
Organism=Escherichia coli, GI1786246, Length=725, Percent_Identity=25.9310344827586, Blast_Score=160, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17559488, Length=748, Percent_Identity=27.4064171122995, Blast_Score=209, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI32565317, Length=622, Percent_Identity=25.7234726688103, Blast_Score=152, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI86563326, Length=626, Percent_Identity=25.0798722044728, Blast_Score=119, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17507143, Length=429, Percent_Identity=24.4755244755245, Blast_Score=66, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6320101, Length=863, Percent_Identity=26.6512166859791, Blast_Score=236, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6324227, Length=640, Percent_Identity=25.78125, Blast_Score=151, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6325090, Length=629, Percent_Identity=24.6422893481717, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24664937, Length=871, Percent_Identity=25.8323765786452, Blast_Score=224, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24648780, Length=620, Percent_Identity=25, Blast_Score=152, Evalue=6e-37,
Organism=Drosophila melanogaster, GI24586371, Length=625, Percent_Identity=23.68, Blast_Score=107, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR017964
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR004578
- InterPro:   IPR023211
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 92089; Mature: 92089

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS00116 DNA_POLYMERASE_B

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIEFYLLDVTYEVVGKEPHVILWGITRNGERVVLRDRRFRPYFYAIINERIDAETVARK
CCEEEEEEEEEHHHHCCCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHCCCCHHHHHHH
IRAFSEAESPIIEVKPTEKKYFGKPVKALRITTVIPEYVRKYRERIREISEVVDVLEADI
HHHHHHCCCCEEEECCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFSMRYLLDHGLEPCGWHIAEVMEAPVPAKYRATRFYEVVSSIKPIGDTRPPKDLRVMAF
HHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
DIEVYSETGTPHPERDPVIIIGGKTSSGEVKQFIAEELSDKDVIAEFTDYILRYDPDIIV
EEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEE
GYNSSRFDWPYLMERARVNNLKLDVGRRRDGQPSQSTYGHISIPGRIHVDLLNFAEEIPE
ECCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEEECCCEEEHHHHHHHHHCCC
VKLKSLDIVADYLGVMKRSERVLIDYIEFPRYWRDPARKPLLLRYNLDDVESTMGLAEKF
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCCCEEEEECCHHHHHHHHHHHHH
LPFAMQLSSITGLPLDQVGAASVGNRLEWFLMREAFRRDELIPNRIEKEVEPYKGAVVLQ
HHHHHHHHHCCCCCHHHCCHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEE
PLPGIHENIAVLDFTSMYPNIMIKYNIGPDTIIREGVCRESEHYVAPEVKHCFLREPPGF
CCCCCCCCEEEEEECCCCCCEEEEEECCCHHHHHHCCCCCCCCCCCCHHHHHHCCCCCHH
FKGVLQILLSLRKQIRGEMKKYKTDSPEYRLLDERQKAIKVLANATYGYMGWVAARWYCK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
ECAEAVTAWGRRTILSVIDYARVIGLKVIYGDTDSLFVENIPDKITRLSEYVERELGFEI
HHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHCCHHHHHHHHHHHHHHCCCEE
KIDKVYKRVFFTEAKKRYAGLLEDGRVDIVGFEAVRGDWAEIAKEVQEKVTEILLSEKNI
EHHHHHHHHHHHHHHHHHHCHHCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCH
EKAIEYVRNIIAELNQGRIPLEKLIIWKTLTKSVEEYEAEAPHVNAARKMKRMGMKIEVG
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEC
DKIGYIVVKGSGKISDRAEPYMFVKDPKLVDTIYYIDHQIIPAALRILEYFGVTDTQLKK
CCEEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
VASTAGRKSLFDYTKKT
HHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MEIEFYLLDVTYEVVGKEPHVILWGITRNGERVVLRDRRFRPYFYAIINERIDAETVARK
CCEEEEEEEEEHHHHCCCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHCCCCHHHHHHH
IRAFSEAESPIIEVKPTEKKYFGKPVKALRITTVIPEYVRKYRERIREISEVVDVLEADI
HHHHHHCCCCEEEECCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFSMRYLLDHGLEPCGWHIAEVMEAPVPAKYRATRFYEVVSSIKPIGDTRPPKDLRVMAF
HHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
DIEVYSETGTPHPERDPVIIIGGKTSSGEVKQFIAEELSDKDVIAEFTDYILRYDPDIIV
EEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEE
GYNSSRFDWPYLMERARVNNLKLDVGRRRDGQPSQSTYGHISIPGRIHVDLLNFAEEIPE
ECCCCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCCCEEEECCCEEEHHHHHHHHHCCC
VKLKSLDIVADYLGVMKRSERVLIDYIEFPRYWRDPARKPLLLRYNLDDVESTMGLAEKF
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCCCEEEEECCHHHHHHHHHHHHH
LPFAMQLSSITGLPLDQVGAASVGNRLEWFLMREAFRRDELIPNRIEKEVEPYKGAVVLQ
HHHHHHHHHCCCCCHHHCCHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCEEEEE
PLPGIHENIAVLDFTSMYPNIMIKYNIGPDTIIREGVCRESEHYVAPEVKHCFLREPPGF
CCCCCCCCEEEEEECCCCCCEEEEEECCCHHHHHHCCCCCCCCCCCCHHHHHHCCCCCHH
FKGVLQILLSLRKQIRGEMKKYKTDSPEYRLLDERQKAIKVLANATYGYMGWVAARWYCK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
ECAEAVTAWGRRTILSVIDYARVIGLKVIYGDTDSLFVENIPDKITRLSEYVERELGFEI
HHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHCCHHHHHHHHHHHHHHCCCEE
KIDKVYKRVFFTEAKKRYAGLLEDGRVDIVGFEAVRGDWAEIAKEVQEKVTEILLSEKNI
EHHHHHHHHHHHHHHHHHHCHHCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCH
EKAIEYVRNIIAELNQGRIPLEKLIIWKTLTKSVEEYEAEAPHVNAARKMKRMGMKIEVG
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEEC
DKIGYIVVKGSGKISDRAEPYMFVKDPKLVDTIYYIDHQIIPAALRILEYFGVTDTQLKK
CCEEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
VASTAGRKSLFDYTKKT
HHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]