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The map label for this gene is tgtA [H]

Identifier: 218884569

GI number: 218884569

Start: 1176759

End: 1178321

Strand: Reverse

Name: tgtA [H]

Synonym: DKAM_1258

Alternate gene names: 218884569

Gene position: 1178321-1176759 (Counterclockwise)

Preceding gene: 218884570

Following gene: 218884565

Centisome position: 86.31

GC content: 43.38

Gene sequence:

>1563_bases
GTGTTTTTCGAGCCACGCGAATATGATTTAGCAGGTAGGATAACCAGGTTAACTACTAGGCATGGAAGTATTGAAACCCC
CTATCTTTTTCCAGTCATCAACCCATTAAAGCAGGAGGTCCCACTTGATAAGCTTTTAGAGCTTGGTTTCAACGGATTAA
TAACAAACGCTTACCTCTTCTACAAGAGGAATAAGGGAGTAGCCAAGCCGATCCACGATGAGTTAAAGTGGAGTAAAACC
ATAATGACGGATTCAGGCGGCTACCAGGTCCTAATATATGGTGATGTAGAGGTCGATAATAAGACTATAGTATCATATCA
GAGGCGCATAGGAGTAGATATAGGCGTGATACTGGATAAGCCCACGGGTAATAGGGCATCATATAAGGAAGCCGTTGAAA
GCATCTATGAAACATATAGAAGAGCTGTTGAAGCTGCGCCACTAATATCCGATAGTGATCAGTTATGGGTGCTCCCTATC
CAGGGAATACCCTATAACGAGCTTGTTGAAAGATCATCGATACTAGCGTGGAAGCTCCCCGTTTACAGCATATACGCTAT
AGGATCTCCTACAGTACTCCTCGAGAAATACAGTTACTCGAAAATAATCGAATCCCTTATCCTAGCTAAAAGATTACTGC
CACCAGGGAAACCCATACATGTATTTGGGGTAGGACACCCGATGGCCCTCCCCTTCCTAGTAGCAGCCGGAGGAGATCTC
TTCGATTCAGCGAGCTATATATTATATGCCCGCGATGATAGGTATATGACTGAGACCGGAACTAGAAACATAAGGGATCT
ACATTACCTACCATGCAACTGCCCTGTCTGCAGTCGTTACACCGCCAAGGAACTACTTGAGCTACCCAGACAGGAGAGAA
CGAGATTAATAGCCTTACATAACCTCTACATGCTCTCCAAGGAATTGAAAAACGTTAAGCAAGCCATCATGGAGGGCAGG
CTCTGGGAGCTCCTGGAGTATAAGAGCAAGGGGCATCCAAGCCTCAGGGAAGCATTCAATGTCCTAGTGAAATATAGAGA
CCTATTGAAGCAGTATAACCCCTTGTCAAAGCCGGATACGAAAGCCCTGTTTCTAATTGATAGATCGTCAACACGTAATC
CCAGAGTAGAGGAAACGAAGACAAGGATCTCCAACATCCTCGGTGACTCCTTAAGGAATAAATACATCATTATAATCCCT
GCCTATAGAAAACCATATACAAGCCAAGATGAATACCTAGACCTAGAAAACAACCTTAGAGGCAATAATTCAATACAAGT
ACTCTTTATACATCCATTCCTAGGAGTAATACCACCAGGGTTATCCTCAACCTATCCGTTCTATCAGCATGAATCACGGT
TCACTACAAGGGATATCTCCCCGATGGAAATAGGCTCATTGCTTTCAACCCTTAGCAGACAAGGAGCCAAGAAGATAATC
ATAGTGGAAGCCAGCTGGTTCACTAGAAAACTATACACCAAAATACTAAGGGCTTTCCCAGACTTAAGGGATAAAATAGC
TATATATAGGATAGACGAGATTTCTTCTCAGATTCCTCTATAG

Upstream 100 bases:

>100_bases
GAGGACGGTGTACTTGGCTCCGGACCTATAGCTGAGACGCTTCACAGCATTTACAAGAAATACATAGAGTACAGAAGGAA
GATGATACAGGGATAGAAAG

Downstream 100 bases:

>100_bases
CCTTTTCAACTTTTTCTCTTTCAGCCTCTATAGCCTCTATAAGGGATGCCTCCTCAAGTAGTGATGAAGTATTTATTTCA
ACACCCAGTATACTACTTAT

Product: 7-cyano-7-deazaguanine tRNA-ribosyltransferase

Products: tRNAqueuine; guanine [C]

Alternate protein names: Archaeal tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MFFEPREYDLAGRITRLTTRHGSIETPYLFPVINPLKQEVPLDKLLELGFNGLITNAYLFYKRNKGVAKPIHDELKWSKT
IMTDSGGYQVLIYGDVEVDNKTIVSYQRRIGVDIGVILDKPTGNRASYKEAVESIYETYRRAVEAAPLISDSDQLWVLPI
QGIPYNELVERSSILAWKLPVYSIYAIGSPTVLLEKYSYSKIIESLILAKRLLPPGKPIHVFGVGHPMALPFLVAAGGDL
FDSASYILYARDDRYMTETGTRNIRDLHYLPCNCPVCSRYTAKELLELPRQERTRLIALHNLYMLSKELKNVKQAIMEGR
LWELLEYKSKGHPSLREAFNVLVKYRDLLKQYNPLSKPDTKALFLIDRSSTRNPRVEETKTRISNILGDSLRNKYIIIIP
AYRKPYTSQDEYLDLENNLRGNNSIQVLFIHPFLGVIPPGLSSTYPFYQHESRFTTRDISPMEIGSLLSTLSRQGAKKII
IVEASWFTRKLYTKILRAFPDLRDKIAIYRIDEISSQIPL

Sequences:

>Translated_520_residues
MFFEPREYDLAGRITRLTTRHGSIETPYLFPVINPLKQEVPLDKLLELGFNGLITNAYLFYKRNKGVAKPIHDELKWSKT
IMTDSGGYQVLIYGDVEVDNKTIVSYQRRIGVDIGVILDKPTGNRASYKEAVESIYETYRRAVEAAPLISDSDQLWVLPI
QGIPYNELVERSSILAWKLPVYSIYAIGSPTVLLEKYSYSKIIESLILAKRLLPPGKPIHVFGVGHPMALPFLVAAGGDL
FDSASYILYARDDRYMTETGTRNIRDLHYLPCNCPVCSRYTAKELLELPRQERTRLIALHNLYMLSKELKNVKQAIMEGR
LWELLEYKSKGHPSLREAFNVLVKYRDLLKQYNPLSKPDTKALFLIDRSSTRNPRVEETKTRISNILGDSLRNKYIIIIP
AYRKPYTSQDEYLDLENNLRGNNSIQVLFIHPFLGVIPPGLSSTYPFYQHESRFTTRDISPMEIGSLLSTLSRQGAKKII
IVEASWFTRKLYTKILRAFPDLRDKIAIYRIDEISSQIPL
>Mature_520_residues
MFFEPREYDLAGRITRLTTRHGSIETPYLFPVINPLKQEVPLDKLLELGFNGLITNAYLFYKRNKGVAKPIHDELKWSKT
IMTDSGGYQVLIYGDVEVDNKTIVSYQRRIGVDIGVILDKPTGNRASYKEAVESIYETYRRAVEAAPLISDSDQLWVLPI
QGIPYNELVERSSILAWKLPVYSIYAIGSPTVLLEKYSYSKIIESLILAKRLLPPGKPIHVFGVGHPMALPFLVAAGGDL
FDSASYILYARDDRYMTETGTRNIRDLHYLPCNCPVCSRYTAKELLELPRQERTRLIALHNLYMLSKELKNVKQAIMEGR
LWELLEYKSKGHPSLREAFNVLVKYRDLLKQYNPLSKPDTKALFLIDRSSTRNPRVEETKTRISNILGDSLRNKYIIIIP
AYRKPYTSQDEYLDLENNLRGNNSIQVLFIHPFLGVIPPGLSSTYPFYQHESRFTTRDISPMEIGSLLSTLSRQGAKKII
IVEASWFTRKLYTKILRAFPDLRDKIAIYRIDEISSQIPL

Specific function: Exchanges the guanine residue with 7-cyano-7- deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D- loop) of archaeal tRNAs [H]

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the archaeosine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1786607, Length=360, Percent_Identity=26.6666666666667, Blast_Score=93, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19920510, Length=377, Percent_Identity=25.4641909814324, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: 2.4.2.29 [C]

Molecular weight: Translated: 59724; Mature: 59724

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFEPREYDLAGRITRLTTRHGSIETPYLFPVINPLKQEVPLDKLLELGFNGLITNAYLF
CCCCCCCCCHHCCEEEEEECCCCCCCCEEHHHHHHHHHHCCHHHHHHCCCCCEEEEEEEE
YKRNKGVAKPIHDELKWSKTIMTDSGGYQVLIYGDVEVDNKTIVSYQRRIGVDIGVILDK
EECCCCCCCCHHHHHHHHHEEEECCCCEEEEEEEEEEECCCHHHHHHHHHCCEEEEEEEC
PTGNRASYKEAVESIYETYRRAVEAAPLISDSDQLWVLPIQGIPYNELVERSSILAWKLP
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHCCEEEEECC
VYSIYAIGSPTVLLEKYSYSKIIESLILAKRLLPPGKPIHVFGVGHPMALPFLVAAGGDL
EEEEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHEECCCCC
FDSASYILYARDDRYMTETGTRNIRDLHYLPCNCPVCSRYTAKELLELPRQERTRLIALH
CCCCCEEEEEECCCEEECCCCCCCCEEEEECCCCCCCCCCCHHHHHHCCHHHHHHHHHHH
NLYMLSKELKNVKQAIMEGRLWELLEYKSKGHPSLREAFNVLVKYRDLLKQYNPLSKPDT
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KALFLIDRSSTRNPRVEETKTRISNILGDSLRNKYIIIIPAYRKPYTSQDEYLDLENNLR
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEECCCCCCC
GNNSIQVLFIHPFLGVIPPGLSSTYPFYQHESRFTTRDISPMEIGSLLSTLSRQGAKKII
CCCCEEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
IVEASWFTRKLYTKILRAFPDLRDKIAIYRIDEISSQIPL
EEECCHHHHHHHHHHHHHCCCHHHCEEEEEEHHHHCCCCC
>Mature Secondary Structure
MFFEPREYDLAGRITRLTTRHGSIETPYLFPVINPLKQEVPLDKLLELGFNGLITNAYLF
CCCCCCCCCHHCCEEEEEECCCCCCCCEEHHHHHHHHHHCCHHHHHHCCCCCEEEEEEEE
YKRNKGVAKPIHDELKWSKTIMTDSGGYQVLIYGDVEVDNKTIVSYQRRIGVDIGVILDK
EECCCCCCCCHHHHHHHHHEEEECCCCEEEEEEEEEEECCCHHHHHHHHHCCEEEEEEEC
PTGNRASYKEAVESIYETYRRAVEAAPLISDSDQLWVLPIQGIPYNELVERSSILAWKLP
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHCCEEEEECC
VYSIYAIGSPTVLLEKYSYSKIIESLILAKRLLPPGKPIHVFGVGHPMALPFLVAAGGDL
EEEEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHEECCCCC
FDSASYILYARDDRYMTETGTRNIRDLHYLPCNCPVCSRYTAKELLELPRQERTRLIALH
CCCCCEEEEEECCCEEECCCCCCCCEEEEECCCCCCCCCCCHHHHHHCCHHHHHHHHHHH
NLYMLSKELKNVKQAIMEGRLWELLEYKSKGHPSLREAFNVLVKYRDLLKQYNPLSKPDT
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KALFLIDRSSTRNPRVEETKTRISNILGDSLRNKYIIIIPAYRKPYTSQDEYLDLENNLR
CEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEECCCCCCC
GNNSIQVLFIHPFLGVIPPGLSSTYPFYQHESRFTTRDISPMEIGSLLSTLSRQGAKKII
CCCCEEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
IVEASWFTRKLYTKILRAFPDLRDKIAIYRIDEISSQIPL
EEECCHHHHHHHHHHHHHCCCHHHCEEEEEEHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn; Mg [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.00006 {guanine}} 0.000053 {guanine}} 0.000014 {7-(aminomethyl)-7-deazaguanine}} [C]

Substrates: tRNAguanine; queuine [C]

Specific reaction: tRNAguanine + queuine = tRNAqueuine + guanine [C]

General reaction: Pentosyl group transfer [C]

Inhibitor: Bisimidoester; Dimethyl suberimidate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]