Click here to switch to the map view.

The map label for this gene is mptN [H]

Identifier: 218884360

GI number: 218884360

Start: 995207

End: 996097

Strand: Direct

Name: mptN [H]

Synonym: DKAM_1049

Alternate gene names: 218884360

Gene position: 995207-996097 (Clockwise)

Preceding gene: 218884359

Following gene: 218884362

Centisome position: 72.9

GC content: 46.46

Gene sequence:

>891_bases
TTGAGGATAGCTGTTGTGGATTATAAAAAAGAGCCTAGGAAGGGCTCACTTGAACTAATGGAGTCGATATCTAGGCATGG
ACATGAACCAGTTTACTTGAAGACACCGATGCTTGATGCTGTGTTAACGCCTGGGGGCATCAAGGTGTACCAGTCCACGG
AGGAGGTAAGTGTTAACGCTGCTATTCTAAGAGGAATAGGCTTCATAACCAGCTTAGAGACTCTAATGAAGAGGATCGGT
GTTCTCGAGGCACTTGCTTCACAAATACCCGTGATCAACGATCCTTCTAAATCCCTTATCGCTAGAGACAAGTGGAGATG
CCTCCTACACCTCTACTTAAAGGGGGTTCCCGTACCAGAGACGCTTGTAACCGAGAACCCCTTTACAGCTATGAGGTATG
TTAAAGACAAGCATATAGTGGTATACAAGCCCCTCATGGGGAGCCTTGGCCTAGGGAGCACGCTAGTGCAGGACCCAGAT
CTCGCATTTAACGTTACAAGGGGATTAATGAATATAGGTTTACCAAGCTACTACCAGGTGTACCTTGAGAAGCCGGGTTA
TGATTACAGGGTTTTCGTAGTTGGCGAAGAAGTAATAGGTGCCATGAAACGTGTTAACCCATATTCTTGGAAGACAAATG
TGGCCCAGGGAGCTGGTGGAGTACCTGTCAGCGAGAAGGAGGAGCCAGAGGTATATGAGCTGGGCTTGAAAGCCGTGAAA
ATACTTGGCCTAGAGTATGCGGGCGTCGATATAGCGTATGATAAGGGTACCGAGAGATACTATATTCTAGAGGTGAACGC
GTTTCCACAATGGGAGGGGTTGAGGAGCGCTACAGGAGTAAATCCGCCCGATTACATTGTAAAATATGTTATCGAAAAAA
TAAAAAAGTAG

Upstream 100 bases:

>100_bases
TGCTGCTCTCTCAGGGTTTACGCTTACCTCAGCCCTTACCGCTACAGGCATATCCTCGGCTCTACTTATACTAGCTGCAT
TAATTGAGAAGGTGTAGGGT

Downstream 100 bases:

>100_bases
AGTAGGCTTAGAAGACTAATGTTTCATCCTGATCTTTACTACTGAGCCCTGGAATTCCTGCTGTATATTTAATCCTCCCT
GAACCCTGTTCTGCAGCTCC

Product: RimK family alpha-L-glutamate ligase

Products: NA

Alternate protein names: H(4)MPT:alpha-L-glutamate ligase [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MRIAVVDYKKEPRKGSLELMESISRHGHEPVYLKTPMLDAVLTPGGIKVYQSTEEVSVNAAILRGIGFITSLETLMKRIG
VLEALASQIPVINDPSKSLIARDKWRCLLHLYLKGVPVPETLVTENPFTAMRYVKDKHIVVYKPLMGSLGLGSTLVQDPD
LAFNVTRGLMNIGLPSYYQVYLEKPGYDYRVFVVGEEVIGAMKRVNPYSWKTNVAQGAGGVPVSEKEEPEVYELGLKAVK
ILGLEYAGVDIAYDKGTERYYILEVNAFPQWEGLRSATGVNPPDYIVKYVIEKIKK

Sequences:

>Translated_296_residues
MRIAVVDYKKEPRKGSLELMESISRHGHEPVYLKTPMLDAVLTPGGIKVYQSTEEVSVNAAILRGIGFITSLETLMKRIG
VLEALASQIPVINDPSKSLIARDKWRCLLHLYLKGVPVPETLVTENPFTAMRYVKDKHIVVYKPLMGSLGLGSTLVQDPD
LAFNVTRGLMNIGLPSYYQVYLEKPGYDYRVFVVGEEVIGAMKRVNPYSWKTNVAQGAGGVPVSEKEEPEVYELGLKAVK
ILGLEYAGVDIAYDKGTERYYILEVNAFPQWEGLRSATGVNPPDYIVKYVIEKIKK
>Mature_296_residues
MRIAVVDYKKEPRKGSLELMESISRHGHEPVYLKTPMLDAVLTPGGIKVYQSTEEVSVNAAILRGIGFITSLETLMKRIG
VLEALASQIPVINDPSKSLIARDKWRCLLHLYLKGVPVPETLVTENPFTAMRYVKDKHIVVYKPLMGSLGLGSTLVQDPD
LAFNVTRGLMNIGLPSYYQVYLEKPGYDYRVFVVGEEVIGAMKRVNPYSWKTNVAQGAGGVPVSEKEEPEVYELGLKAVK
ILGLEYAGVDIAYDKGTERYYILEVNAFPQWEGLRSATGVNPPDYIVKYVIEKIKK

Specific function: Catalyzes the ATP or GTP-dependent addition of one L- glutamate molecule to tetrahydromethanopterin, producing tetrahydrosarcinapterin [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1787076, Length=194, Percent_Identity=27.319587628866, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 33041; Mature: 33041

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAVVDYKKEPRKGSLELMESISRHGHEPVYLKTPMLDAVLTPGGIKVYQSTEEVSVNA
CEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEECCCHHHHCCCCCEEEEECCCHHHHHH
AILRGIGFITSLETLMKRIGVLEALASQIPVINDPSKSLIARDKWRCLLHLYLKGVPVPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCH
TLVTENPFTAMRYVKDKHIVVYKPLMGSLGLGSTLVQDPDLAFNVTRGLMNIGLPSYYQV
HHCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCEEEE
YLEKPGYDYRVFVVGEEVIGAMKRVNPYSWKTNVAQGAGGVPVSEKEEPEVYELGLKAVK
EEECCCCCEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ILGLEYAGVDIAYDKGTERYYILEVNAFPQWEGLRSATGVNPPDYIVKYVIEKIKK
HHCEEECCEEEEEECCCCEEEEEEECCCCCCCCCHHCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIAVVDYKKEPRKGSLELMESISRHGHEPVYLKTPMLDAVLTPGGIKVYQSTEEVSVNA
CEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEECCCHHHHCCCCCEEEEECCCHHHHHH
AILRGIGFITSLETLMKRIGVLEALASQIPVINDPSKSLIARDKWRCLLHLYLKGVPVPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCH
TLVTENPFTAMRYVKDKHIVVYKPLMGSLGLGSTLVQDPDLAFNVTRGLMNIGLPSYYQV
HHCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCEEEE
YLEKPGYDYRVFVVGEEVIGAMKRVNPYSWKTNVAQGAGGVPVSEKEEPEVYELGLKAVK
EEECCCCCEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ILGLEYAGVDIAYDKGTERYYILEVNAFPQWEGLRSATGVNPPDYIVKYVIEKIKK
HHCEEECCEEEEEECCCCEEEEEEECCCCCCCCCHHCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]