Click here to switch to the map view.

The map label for this gene is ndhK [H]

Identifier: 218884266

GI number: 218884266

Start: 902074

End: 903087

Strand: Reverse

Name: ndhK [H]

Synonym: DKAM_0955

Alternate gene names: 218884266

Gene position: 903087-902074 (Counterclockwise)

Preceding gene: 218884267

Following gene: 218884265

Centisome position: 66.15

GC content: 45.46

Gene sequence:

>1014_bases
ATGCTTGAGAGGATTGCTAGATGGGCTTACGCTAGAAGCCTCTGGATGATACACTACTGTTCAGCATGTGGAGCAGTAGA
GTTCCCGCCATTAGTAATGGCGCCTCTTGACTGGGAGAGATACGGTTATATGCCAACCCCCTCACCACGTCAGAGCGATC
TATATATAGGTATGGGTTATCTAACAAAGAAGACAGTCAAACTTGTTTTAAACATGTACAGGCAGATGCCGGAGCCCAAG
CTTGTATTAGCCGGTTGCAACTGTACTTCAACAGGTGGACCATACTGGGATAGCTATGCGACATACAAGAGGCTCGATGA
CTTCATCGAGGTAGAGGGATGGGTACCTGGTTGTATGCCGATGCCCGATGATTATTTATCCATGATCGAGTATGTTAGGA
AAGATCTCGGGAAGCGCCCGTTAAACGCTTACGTGTCTAAGATAAAACCAGATGCGTTCAAAAAGATAAGTGAATGGGAG
GAGCTTGAGAAACAGTGGAGGAAAGAATACGAGGAGAAAATCAAGGAAGACTCATCTAAGCCCGTAAGCTACGAGTTCAA
GGAGACCTACCCCTCATGCTACGAGAAAGACGAGAAATCAAAGATATGTAAGACTAGTGTAAACCCGGAGAAGATCAGGG
ATGCACTAATGGATTTAAAGAACAAGGGACATGTACTCTTCGTAAATATTAACACGGTAGATTATCCGGACAAGGGGGTC
ATCGAGGTATACTATGTACTCGAGAACCCCTCTGATGGATCACAGGTATGGGTAAAGACATATGCGAAGAGGGCTAGCCC
CGAGATAGATTCAGTCCACGATATATTTCCGCTGGCCAAGTACATTGAGAGAGAAGTATACGAGTTCATGGGGGTAGTAT
TCAGGAATAATCCCGAATTAAAGAAGTGGATCCTAGACGGTAACTGGGAGGGGCCGCCCCCATTAAGGAAAGATGTTGAC
ACAGCTGGTTTCGTTATGAAAACAATGTATGGAGGGTATAAGTATGGCAGGTAA

Upstream 100 bases:

>100_bases
ATGGCCCAGGGATTCATTGAGCCCGCCTCCCAATCACTTATATACGAGAGGTTCAACTACGTTATGAAAGCCCTTTCAGC
TATCGGGAGGTGAGTATGAA

Downstream 100 bases:

>100_bases
AGGTAGACTCGTAGAGATCTACTTTGGTCCGCAGCACCCCGGTGCACCGGGTAATATAGCATTTAAACTAACACTTGACG
GTGAAAGAGTAGTCAACGTG

Product: NADH-quinone oxidoreductase subunit B

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit K; NDH-1 subunit K; NDH-K [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MLERIARWAYARSLWMIHYCSACGAVEFPPLVMAPLDWERYGYMPTPSPRQSDLYIGMGYLTKKTVKLVLNMYRQMPEPK
LVLAGCNCTSTGGPYWDSYATYKRLDDFIEVEGWVPGCMPMPDDYLSMIEYVRKDLGKRPLNAYVSKIKPDAFKKISEWE
ELEKQWRKEYEEKIKEDSSKPVSYEFKETYPSCYEKDEKSKICKTSVNPEKIRDALMDLKNKGHVLFVNINTVDYPDKGV
IEVYYVLENPSDGSQVWVKTYAKRASPEIDSVHDIFPLAKYIEREVYEFMGVVFRNNPELKKWILDGNWEGPPPLRKDVD
TAGFVMKTMYGGYKYGR

Sequences:

>Translated_337_residues
MLERIARWAYARSLWMIHYCSACGAVEFPPLVMAPLDWERYGYMPTPSPRQSDLYIGMGYLTKKTVKLVLNMYRQMPEPK
LVLAGCNCTSTGGPYWDSYATYKRLDDFIEVEGWVPGCMPMPDDYLSMIEYVRKDLGKRPLNAYVSKIKPDAFKKISEWE
ELEKQWRKEYEEKIKEDSSKPVSYEFKETYPSCYEKDEKSKICKTSVNPEKIRDALMDLKNKGHVLFVNINTVDYPDKGV
IEVYYVLENPSDGSQVWVKTYAKRASPEIDSVHDIFPLAKYIEREVYEFMGVVFRNNPELKKWILDGNWEGPPPLRKDVD
TAGFVMKTMYGGYKYGR
>Mature_337_residues
MLERIARWAYARSLWMIHYCSACGAVEFPPLVMAPLDWERYGYMPTPSPRQSDLYIGMGYLTKKTVKLVLNMYRQMPEPK
LVLAGCNCTSTGGPYWDSYATYKRLDDFIEVEGWVPGCMPMPDDYLSMIEYVRKDLGKRPLNAYVSKIKPDAFKKISEWE
ELEKQWRKEYEEKIKEDSSKPVSYEFKETYPSCYEKDEKSKICKTSVNPEKIRDALMDLKNKGHVLFVNINTVDYPDKGV
IEVYYVLENPSDGSQVWVKTYAKRASPEIDSVHDIFPLAKYIEREVYEFMGVVFRNNPELKKWILDGNWEGPPPLRKDVD
TAGFVMKTMYGGYKYGR

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=137, Percent_Identity=33.5766423357664, Blast_Score=85, Evalue=9e-17,
Organism=Escherichia coli, GI1788624, Length=144, Percent_Identity=32.6388888888889, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1789074, Length=128, Percent_Identity=29.6875, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788834, Length=124, Percent_Identity=28.2258064516129, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17509685, Length=138, Percent_Identity=31.8840579710145, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI18859983, Length=137, Percent_Identity=32.8467153284672, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24642371, Length=137, Percent_Identity=32.8467153284672, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24651058, Length=135, Percent_Identity=34.0740740740741, Blast_Score=81, Evalue=8e-16,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 39341; Mature: 39341

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLERIARWAYARSLWMIHYCSACGAVEFPPLVMAPLDWERYGYMPTPSPRQSDLYIGMGY
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHCCCCCCCCCCCCCEEEEHHH
LTKKTVKLVLNMYRQMPEPKLVLAGCNCTSTGGPYWDSYATYKRLDDFIEVEGWVPGCMP
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
MPDDYLSMIEYVRKDLGKRPLNAYVSKIKPDAFKKISEWEELEKQWRKEYEEKIKEDSSK
CCHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PVSYEFKETYPSCYEKDEKSKICKTSVNPEKIRDALMDLKNKGHVLFVNINTVDYPDKGV
CCCEEHHHHCCHHHCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCE
IEVYYVLENPSDGSQVWVKTYAKRASPEIDSVHDIFPLAKYIEREVYEFMGVVFRNNPEL
EEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
KKWILDGNWEGPPPLRKDVDTAGFVMKTMYGGYKYGR
HHEEECCCCCCCCCHHHCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLERIARWAYARSLWMIHYCSACGAVEFPPLVMAPLDWERYGYMPTPSPRQSDLYIGMGY
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHCCCCCCCCCCCCCEEEEHHH
LTKKTVKLVLNMYRQMPEPKLVLAGCNCTSTGGPYWDSYATYKRLDDFIEVEGWVPGCMP
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
MPDDYLSMIEYVRKDLGKRPLNAYVSKIKPDAFKKISEWEELEKQWRKEYEEKIKEDSSK
CCHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PVSYEFKETYPSCYEKDEKSKICKTSVNPEKIRDALMDLKNKGHVLFVNINTVDYPDKGV
CCCEEHHHHCCHHHCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCE
IEVYYVLENPSDGSQVWVKTYAKRASPEIDSVHDIFPLAKYIEREVYEFMGVVFRNNPEL
EEEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
KKWILDGNWEGPPPLRKDVDTAGFVMKTMYGGYKYGR
HHEEECCCCCCCCCHHHCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA