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The map label for this gene is aegA [H]

Identifier: 218883414

GI number: 218883414

Start: 86839

End: 88251

Strand: Direct

Name: aegA [H]

Synonym: DKAM_0100

Alternate gene names: 218883414

Gene position: 86839-88251 (Clockwise)

Preceding gene: 218883413

Following gene: 218883419

Centisome position: 6.36

GC content: 60.08

Gene sequence:

>1413_bases
GTGGTCAAGAAGAGGTTTACACCTAAGGTCCTGCCCGTAAGCGAGAGGCTGAGGAGCTTCGGCGAGGTGGTCTTGGGCTA
CGACTACGAGACTTCTGTTAGAGAGGCCTCGAGGTGCCTCCGCTGCCCCCTCTGGTTCGCCCCATGCTCGAAGGCGTGCC
CGTTGAGGGTCAAAGTCCCGGCCTTCCTAGAGTACATTGTCAAGGGGGACCTCGAGGCGGCCGCCAGGAAGGTCTACGAG
GACAACCTCTTCCCCTCCATAACGGGCAGGGTCTGCCCCCAGGAGTGGTTCTGTGAAGGCAACTGCGTAGTCTCCAAGAT
AGGCGAGAGCGTCTCCGTCGGCCTCTTAGAGAGGTACGTCGGAGACTACGCGAGGGAGAATGGACTGGACGAGAAGATCC
TGGCCGAGATGGTCTCTAAGAACAGTGGGGTCAAGGGAAGGGTAGCGGTGGTAGGCTCGGGCCCCGCCGGGCTGTCAGCG
GCTGGCTGGCTTAGGTTGAGAGGTTATGAAGTCGTCGTATACGAGGCCCTCCACAGGCCTGGGGGTGTGCTGGCGTACGG
GATACCCGAGTTCAGGTTGCCCAGGAGCGTCCTAGACCACGAGATCAAGAAGGTCGAGAACCTAGGCGTTGAGATAAGGC
TGAACCACGTGGTGGGGAAAACCGTGCTGCTAAGCGAGCTATTGAGCGAGTACGACGCCGTCTTTATTGGCAGCGGCGCC
GGTACACCCAAGCTACTCAACATCCCGGGCGTCGACTTGAACAACATCTACACGGCTAACGAGTTCCTCACCAGGGTCAA
CCTAATGAGGGCGCACCTCTTCCCAGAGTGGGACACACCGATCAGCGTCGGGAAGACCACGTTGATAATCGGGGGAGGTA
ACACCGCCATGGACGCAGCGAGGGTAGCTCTGAGACTGGGCAGCAAGCCGGTCGTCGTCTACAGGAGGGACAGGGAGGAC
ATGTTGAAGACGACTAGGAGGGACGAGGTGCTGCACGCGGAAGAGGAGGGCGTGGAGTTCAAGTACTACCTGCAGCCGAT
CGAGTTCCTCGGCGACGAGAACGGCTTCGTTAAGGCTGTGAGGTTCGAGGTAATGGAGCCCCTCAACGAGCTCGACGAGA
GGGGCAAGAAGAAGATCGTCGGGACGGGTAGGTACGTCGAGATCCCCGCGGACACGGTGGTTATAGCGATAGGGCTGGAA
CCCAACAGGTTGCTCATGGAGGAGAGCGGGCTGAGGTTCAACGAGGACGGGACGATAGCCGTAGACGACCACCTCATGAT
GAGCCTGCCCGGCGTGTTCGCGGGGGGCGACGCTATAAGAGGCGAGTCGACGGTCGTCGAGGCCATGGCAGACGGGAAGA
GGGCTGCGGAAGAGATAGACCTGTACGTGAGGAGGAAGAAGGGACTAGCGTAG

Upstream 100 bases:

>100_bases
GGTCGACTGGGACGACGTCCTGAGGAGGGACGCGAGGTTCAAGAAGGTCGAGAAGATCGCACTGGACAAGTACCTGGCGA
GGTGAGCTGTCTTGTCCCGA

Downstream 100 bases:

>100_bases
GGCACTTTTCTCGTACAGCTCTCTATTGAGTATTTTTGTCAACGCCATCAGGGTGGTCAGCCTCGACAGCTCGGACCTCA
CTACGACGTGCCTGGTCAGG

Product: Ferredoxin:NADP oxidoreductase subunit alpha

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MVKKRFTPKVLPVSERLRSFGEVVLGYDYETSVREASRCLRCPLWFAPCSKACPLRVKVPAFLEYIVKGDLEAAARKVYE
DNLFPSITGRVCPQEWFCEGNCVVSKIGESVSVGLLERYVGDYARENGLDEKILAEMVSKNSGVKGRVAVVGSGPAGLSA
AGWLRLRGYEVVVYEALHRPGGVLAYGIPEFRLPRSVLDHEIKKVENLGVEIRLNHVVGKTVLLSELLSEYDAVFIGSGA
GTPKLLNIPGVDLNNIYTANEFLTRVNLMRAHLFPEWDTPISVGKTTLIIGGGNTAMDAARVALRLGSKPVVVYRRDRED
MLKTTRRDEVLHAEEEGVEFKYYLQPIEFLGDENGFVKAVRFEVMEPLNELDERGKKKIVGTGRYVEIPADTVVIAIGLE
PNRLLMEESGLRFNEDGTIAVDDHLMMSLPGVFAGGDAIRGESTVVEAMADGKRAAEEIDLYVRRKKGLA

Sequences:

>Translated_470_residues
MVKKRFTPKVLPVSERLRSFGEVVLGYDYETSVREASRCLRCPLWFAPCSKACPLRVKVPAFLEYIVKGDLEAAARKVYE
DNLFPSITGRVCPQEWFCEGNCVVSKIGESVSVGLLERYVGDYARENGLDEKILAEMVSKNSGVKGRVAVVGSGPAGLSA
AGWLRLRGYEVVVYEALHRPGGVLAYGIPEFRLPRSVLDHEIKKVENLGVEIRLNHVVGKTVLLSELLSEYDAVFIGSGA
GTPKLLNIPGVDLNNIYTANEFLTRVNLMRAHLFPEWDTPISVGKTTLIIGGGNTAMDAARVALRLGSKPVVVYRRDRED
MLKTTRRDEVLHAEEEGVEFKYYLQPIEFLGDENGFVKAVRFEVMEPLNELDERGKKKIVGTGRYVEIPADTVVIAIGLE
PNRLLMEESGLRFNEDGTIAVDDHLMMSLPGVFAGGDAIRGESTVVEAMADGKRAAEEIDLYVRRKKGLA
>Mature_470_residues
MVKKRFTPKVLPVSERLRSFGEVVLGYDYETSVREASRCLRCPLWFAPCSKACPLRVKVPAFLEYIVKGDLEAAARKVYE
DNLFPSITGRVCPQEWFCEGNCVVSKIGESVSVGLLERYVGDYARENGLDEKILAEMVSKNSGVKGRVAVVGSGPAGLSA
AGWLRLRGYEVVVYEALHRPGGVLAYGIPEFRLPRSVLDHEIKKVENLGVEIRLNHVVGKTVLLSELLSEYDAVFIGSGA
GTPKLLNIPGVDLNNIYTANEFLTRVNLMRAHLFPEWDTPISVGKTTLIIGGGNTAMDAARVALRLGSKPVVVYRRDRED
MLKTTRRDEVLHAEEEGVEFKYYLQPIEFLGDENGFVKAVRFEVMEPLNELDERGKKKIVGTGRYVEIPADTVVIAIGLE
PNRLLMEESGLRFNEDGTIAVDDHLMMSLPGVFAGGDAIRGESTVVEAMADGKRAAEEIDLYVRRKKGLA

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=456, Percent_Identity=30.4824561403509, Blast_Score=167, Evalue=1e-41,
Organism=Escherichia coli, GI1788811, Length=464, Percent_Identity=36.2068965517241, Blast_Score=271, Evalue=5e-74,
Organism=Escherichia coli, GI1789606, Length=470, Percent_Identity=34.8936170212766, Blast_Score=251, Evalue=5e-68,
Organism=Escherichia coli, GI87082180, Length=469, Percent_Identity=35.181236673774, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1788468, Length=432, Percent_Identity=33.3333333333333, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1789243, Length=418, Percent_Identity=29.6650717703349, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17570289, Length=480, Percent_Identity=31.25, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI71984108, Length=449, Percent_Identity=30.5122494432071, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17543860, Length=162, Percent_Identity=33.9506172839506, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320030, Length=454, Percent_Identity=29.9559471365639, Blast_Score=176, Evalue=6e-45,
Organism=Drosophila melanogaster, GI24665547, Length=456, Percent_Identity=33.5526315789474, Blast_Score=211, Evalue=8e-55,
Organism=Drosophila melanogaster, GI24665543, Length=456, Percent_Identity=33.5526315789474, Blast_Score=211, Evalue=8e-55,
Organism=Drosophila melanogaster, GI28574881, Length=456, Percent_Identity=33.5526315789474, Blast_Score=211, Evalue=9e-55,
Organism=Drosophila melanogaster, GI24665539, Length=456, Percent_Identity=33.5526315789474, Blast_Score=211, Evalue=9e-55,
Organism=Drosophila melanogaster, GI24640763, Length=447, Percent_Identity=31.0961968680089, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI18858217, Length=447, Percent_Identity=31.0961968680089, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 51967; Mature: 51967

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKKRFTPKVLPVSERLRSFGEVVLGYDYETSVREASRCLRCPLWFAPCSKACPLRVKVP
CCCCCCCCCCCCHHHHHHHHCCEEEECCCCHHHHHHHHHHCCCCEECCCCCCCCEEEEHH
AFLEYIVKGDLEAAARKVYEDNLFPSITGRVCPQEWFCEGNCVVSKIGESVSVGLLERYV
HHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCEEHHHCCCHHHHHHHHHHH
GDYARENGLDEKILAEMVSKNSGVKGRVAVVGSGPAGLSAAGWLRLRGYEVVVYEALHRP
HHHHHHCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEECCEEEEEEEHHCCC
GGVLAYGIPEFRLPRSVLDHEIKKVENLGVEIRLNHVVGKTVLLSELLSEYDAVFIGSGA
CCEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHCCEEEEECCC
GTPKLLNIPGVDLNNIYTANEFLTRVNLMRAHLFPEWDTPISVGKTTLIIGGGNTAMDAA
CCCEEEECCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHH
RVALRLGSKPVVVYRRDREDMLKTTRRDEVLHAEEEGVEFKYYLQPIEFLGDENGFVKAV
HHHHHCCCCCEEEEECCHHHHHHHHHHHHHHEHHHCCCEEEEEECHHHHHCCCCCHHHHH
RFEVMEPLNELDERGKKKIVGTGRYVEIPADTVVIAIGLEPNRLLMEESGLRFNEDGTIA
HHHHHHHHHHHHHCCCEEEEECCCEEEECCCEEEEEEECCCCEEEEECCCCEECCCCCEE
VDDHLMMSLPGVFAGGDAIRGESTVVEAMADGKRAAEEIDLYVRRKKGLA
ECCCHHHHCCCEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVKKRFTPKVLPVSERLRSFGEVVLGYDYETSVREASRCLRCPLWFAPCSKACPLRVKVP
CCCCCCCCCCCCHHHHHHHHCCEEEECCCCHHHHHHHHHHCCCCEECCCCCCCCEEEEHH
AFLEYIVKGDLEAAARKVYEDNLFPSITGRVCPQEWFCEGNCVVSKIGESVSVGLLERYV
HHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCEEHHHCCCHHHHHHHHHHH
GDYARENGLDEKILAEMVSKNSGVKGRVAVVGSGPAGLSAAGWLRLRGYEVVVYEALHRP
HHHHHHCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCEEEECCEEEEEEEHHCCC
GGVLAYGIPEFRLPRSVLDHEIKKVENLGVEIRLNHVVGKTVLLSELLSEYDAVFIGSGA
CCEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHCCEEEEECCC
GTPKLLNIPGVDLNNIYTANEFLTRVNLMRAHLFPEWDTPISVGKTTLIIGGGNTAMDAA
CCCEEEECCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHH
RVALRLGSKPVVVYRRDREDMLKTTRRDEVLHAEEEGVEFKYYLQPIEFLGDENGFVKAV
HHHHHCCCCCEEEEECCHHHHHHHHHHHHHHEHHHCCCEEEEEECHHHHHCCCCCHHHHH
RFEVMEPLNELDERGKKKIVGTGRYVEIPADTVVIAIGLEPNRLLMEESGLRFNEDGTIA
HHHHHHHHHHHHHCCCEEEEECCCEEEECCCEEEEEEECCCCEEEEECCCCEECCCCCEE
VDDHLMMSLPGVFAGGDAIRGESTVVEAMADGKRAAEEIDLYVRRKKGLA
ECCCHHHHCCCEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]