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The map label for this gene is pstS [H]

Identifier: 218883382

GI number: 218883382

Start: 60569

End: 61693

Strand: Reverse

Name: pstS [H]

Synonym: DKAM_0068

Alternate gene names: 218883382

Gene position: 61693-60569 (Counterclockwise)

Preceding gene: 218883384

Following gene: 218883380

Centisome position: 4.52

GC content: 44.71

Gene sequence:

>1125_bases
ATGAAGGAGCCATTACTGCAGTCAATGGCGATTATTATGACTGTTCTCGCGCTCACAATAGCATTATACTTTATAGTTCT
AGCACCCGATACACAGTTAGAGGGATATCATGGAACCCTTGTTATAAATGGAGCCGGCTCAACGCTTATAGCCCCACAGA
TACTTGAATGGAGTAAAGAATTCACCGAGAAAACCGGCATAGTTATCGAGTACTCTCCCACTGGTAGTGGAGCCGGTAGA
GCAATGTTCTTCAATAAGACAGTGGTATTCGCGGTTAGCGACCCCCCATTGGATAGGGAGACATATCTTGAATACCGGGG
CAGGGTACTCCAGTTGCCCATAGTGTTAAGCGCTATAGTTATCACCTACAATATACCTGGAATACCACGCGATATACAAT
TAAACCTGACTGGTAGAATTCTCGCGTTGATATACAAGGGTGAGATCACTAGATGGAACGATGAGAGAATTAAGGAGGTC
AATCCAGCGATCGCCGATCTACTACCCAATAAAGAAATAGTGGTTGTGCACAGGTCGGATTCTAGTGGGACAACACGTGT
CTTCGCCGCTTACCTCAACAAGGCTGCACCTGATATATGGGGGAACGAGCTTGTTGGTTTTTCAATCGAGTGGCCAGTAG
ATAGAACCGGCAGAGGCCTCGGTGGGAAAGGTAATGAGGGGGTTGCAAACCTTGTAACATCAACTCCCTACTCTATAGGC
TATATTGAGGCCAGCTACGCGTTATATGTTGGAATGCCAGTAGCTAGACTAGAGAATAAAGAAGGATATTTTGTTACAGC
ATCCAGGGAGAATGTTCTCTCAGCTGTAAAACATGTTCTGAATCAATTACCGGGTAGTCCACTGGAAGATTTTAGCATGG
TACTTGACGCGTTACTAAACACGCCCGGCCCAGACTCATACCCTATTGTATCATTCTCATACATGTTTATTTACAGTGAC
TATAAAGATGTATATGTTACTGAGGCATTAAGCAGGCTCATAGAATATATTCTAATAGAGGGGCAGGATAGACTACTCCC
CGGCTACTATCAGCTCCCCGATGAAGTCATCGAGATAGGCCTCAAAGCACTTGAAATTATCTCGAGGGGAAGCTATAATG
GGTAA

Upstream 100 bases:

>100_bases
AATACCGTAAATATAGTTTGTACACGCTTCTTACAGTAAGAACTGTAATAATATCCATGCTTTAACCTGGATACACAGTA
ATCATTATCAGGGGAATATT

Downstream 100 bases:

>100_bases
GAGCGAGTACACCTTCTACTTACTCCTCATCCCATCCCTACTCTCATTCTCTATTCTCACACTTATCCTCATACACGTTG
TCACCCTATCCCTGCCAGCA

Product: phosphate ABC transporter, phosphate binding protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKEPLLQSMAIIMTVLALTIALYFIVLAPDTQLEGYHGTLVINGAGSTLIAPQILEWSKEFTEKTGIVIEYSPTGSGAGR
AMFFNKTVVFAVSDPPLDRETYLEYRGRVLQLPIVLSAIVITYNIPGIPRDIQLNLTGRILALIYKGEITRWNDERIKEV
NPAIADLLPNKEIVVVHRSDSSGTTRVFAAYLNKAAPDIWGNELVGFSIEWPVDRTGRGLGGKGNEGVANLVTSTPYSIG
YIEASYALYVGMPVARLENKEGYFVTASRENVLSAVKHVLNQLPGSPLEDFSMVLDALLNTPGPDSYPIVSFSYMFIYSD
YKDVYVTEALSRLIEYILIEGQDRLLPGYYQLPDEVIEIGLKALEIISRGSYNG

Sequences:

>Translated_374_residues
MKEPLLQSMAIIMTVLALTIALYFIVLAPDTQLEGYHGTLVINGAGSTLIAPQILEWSKEFTEKTGIVIEYSPTGSGAGR
AMFFNKTVVFAVSDPPLDRETYLEYRGRVLQLPIVLSAIVITYNIPGIPRDIQLNLTGRILALIYKGEITRWNDERIKEV
NPAIADLLPNKEIVVVHRSDSSGTTRVFAAYLNKAAPDIWGNELVGFSIEWPVDRTGRGLGGKGNEGVANLVTSTPYSIG
YIEASYALYVGMPVARLENKEGYFVTASRENVLSAVKHVLNQLPGSPLEDFSMVLDALLNTPGPDSYPIVSFSYMFIYSD
YKDVYVTEALSRLIEYILIEGQDRLLPGYYQLPDEVIEIGLKALEIISRGSYNG
>Mature_374_residues
MKEPLLQSMAIIMTVLALTIALYFIVLAPDTQLEGYHGTLVINGAGSTLIAPQILEWSKEFTEKTGIVIEYSPTGSGAGR
AMFFNKTVVFAVSDPPLDRETYLEYRGRVLQLPIVLSAIVITYNIPGIPRDIQLNLTGRILALIYKGEITRWNDERIKEV
NPAIADLLPNKEIVVVHRSDSSGTTRVFAAYLNKAAPDIWGNELVGFSIEWPVDRTGRGLGGKGNEGVANLVTSTPYSIG
YIEASYALYVGMPVARLENKEGYFVTASRENVLSAVKHVLNQLPGSPLEDFSMVLDALLNTPGPDSYPIVSFSYMFIYSD
YKDVYVTEALSRLIEYILIEGQDRLLPGYYQLPDEVIEIGLKALEIISRGSYNG

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

Organism=Escherichia coli, GI2367271, Length=319, Percent_Identity=33.5423197492163, Blast_Score=170, Evalue=1e-43,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005673
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 41300; Mature: 41300

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEPLLQSMAIIMTVLALTIALYFIVLAPDTQLEGYHGTLVINGAGSTLIAPQILEWSKE
CCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHH
FTEKTGIVIEYSPTGSGAGRAMFFNKTVVFAVSDPPLDRETYLEYRGRVLQLPIVLSAIV
HHHHCCEEEEECCCCCCCCEEEEEEEEEEEEECCCCCCHHHHHHHCCCEEEHHHHHEEHH
ITYNIPGIPRDIQLNLTGRILALIYKGEITRWNDERIKEVNPAIADLLPNKEIVVVHRSD
HEECCCCCCCEEEEEECCEEEEEEEECCCCCCCHHHHHHCCHHHHHHCCCCCEEEEECCC
SSGTTRVFAAYLNKAAPDIWGNELVGFSIEWPVDRTGRGLGGKGNEGVANLVTSTPYSIG
CCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCEEE
YIEASYALYVGMPVARLENKEGYFVTASRENVLSAVKHVLNQLPGSPLEDFSMVLDALLN
EEEECEEEEECCCHHHCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
TPGPDSYPIVSFSYMFIYSDYKDVYVTEALSRLIEYILIEGQDRLLPGYYQLPDEVIEIG
CCCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHEECCCCCCCCCHHHCCHHHHHHH
LKALEIISRGSYNG
HHHHHHHHCCCCCC
>Mature Secondary Structure
MKEPLLQSMAIIMTVLALTIALYFIVLAPDTQLEGYHGTLVINGAGSTLIAPQILEWSKE
CCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHH
FTEKTGIVIEYSPTGSGAGRAMFFNKTVVFAVSDPPLDRETYLEYRGRVLQLPIVLSAIV
HHHHCCEEEEECCCCCCCCEEEEEEEEEEEEECCCCCCHHHHHHHCCCEEEHHHHHEEHH
ITYNIPGIPRDIQLNLTGRILALIYKGEITRWNDERIKEVNPAIADLLPNKEIVVVHRSD
HEECCCCCCCEEEEEECCEEEEEEEECCCCCCCHHHHHHCCHHHHHHCCCCCEEEEECCC
SSGTTRVFAAYLNKAAPDIWGNELVGFSIEWPVDRTGRGLGGKGNEGVANLVTSTPYSIG
CCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCEEE
YIEASYALYVGMPVARLENKEGYFVTASRENVLSAVKHVLNQLPGSPLEDFSMVLDALLN
EEEECEEEEECCCHHHCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHC
TPGPDSYPIVSFSYMFIYSDYKDVYVTEALSRLIEYILIEGQDRLLPGYYQLPDEVIEIG
CCCCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHEECCCCCCCCCHHHCCHHHHHHH
LKALEIISRGSYNG
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]