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The map label for this gene is glyS [H]

Identifier: 218883366

GI number: 218883366

Start: 47489

End: 49240

Strand: Reverse

Name: glyS [H]

Synonym: DKAM_0052

Alternate gene names: 218883366

Gene position: 49240-47489 (Counterclockwise)

Preceding gene: 218883368

Following gene: 218883365

Centisome position: 3.61

GC content: 43.66

Gene sequence:

>1752_bases
TTGAGTAATGGAGAAGACATATATGGTAACATAATGGAATTAGCAAAGAAACGTGGTATATTCTGGGGAAGCTTCGACAT
ATATGGTGGGTTAGCAGGCTTCTATGATTTCGGTCCGGTTGGAGTACTCCTCAAGAGGAATTTAATCAACCTATGGTTGA
AGACATTCGTATACTCTAATGACCTAGTTGTGGAGATTGAAACACCAGTGATTAACCCCAGGATAGTATTTAAGGCAAGC
GGACACGAGGAAAGCTTCATGGATCCTGTTGTAGAATGCCTTAAATGCGGTAGAGTATACAGGGCTGATCACCTGGTTAA
GGAGGCAGCAGGCATAGATGTAGAGGGTTTAAGCCCCGAGGAAATACACAGTGTTATCCTGAGAAACAATATCAAGTGCC
CCGAGTGCGGTGGTGATCTAGGTAAACCCTTCTATACCCTCCTATTGTTTAAAACAGAGATAGGCCCATACAAGGGGGAA
ATAGGCTACCTCAGGCCCGAGAACGCTCAAGGGATGTTCATAAATTTCGCGAATATTCTCAGAGTAGCTAGAAACAGGCT
TCCACTAGGCATAGCACAGGTCGGCAAGGTTGCTAGAAACGAGATATCGCCACGCCAAGGGTTACTCAGGCTCAGGGAGT
TCACTATAATGGAGCTCGAGTTCTTCTTCGATCCAGAAGAGGTGGAAGAGGCAAGTGGATACATTAATAGCATTGCAGAT
GTAAAAGTAAGAGTGCTCACTAGTGAAGCTAGAGTGAGGGGTGTAGAAGAACCAGTTGAATATACTATTAGAGAACTAGT
TGAGAAACAGATAGTTAAAACCCCATGGCTTGTCTACTGGATTGGTTTAGGAGAGGTGTTTGCGAGAAGACTTGGAATAC
CACATGAAAAAATCAGGTTCATCGAGAAGCTACCTCATGAAAGAGCCCATTACTCCCAGCAGACATTCGACCAGGAAGTA
TACACCGAGAAATATGGATGGATAGAGATAGCTGGATACGCTTATAGAACGACATACGATTTAAGCAGACATATACAGTA
TAGTAAAGCGGACCTAACGTTCTTTAAACACTATGAGAAACCAGTTGAAAGAATTATCCCAAAAGCATATCCTGATCCAG
GTAAAATAAAAGACCTCTATGGCGGGGAAATGGGAAGAATAATGGAAAGACTCGCTCAGAAAACTCCTGAGGAGATCTAT
AGAGAGCTGGCTGAAAAAGGATATGTCGAAATAGAGGGGCTTAAGATGCCTAGTGAAGCCTTCATAGTGAAGCAGGAAAG
GGAGAAAATACATGGAGAGAAAATCATTCCACACGTCGTAGAACCATCATTCGGGGTTGAGAGGCTCCTATATATAGTCC
TTGAATACTCGTACACGGTGAGGGATGGTAAGACAGTTCTAAAACTACCAGTACACCTAGCCCCCTACCATGTAGCAGTA
TTCCCATTAGTAACGGGCAAAAAGCAAGAGCATGCGAGGATAATTGAAATAGCTAGAAACATATATAGAAGACTCATTAC
AAGAGGATTCAGAGTTATCTACGATGATGATGGAAGCATCGGTAGGAGGTATGCCAGGGTCGATGAGATAGGCATCCCAT
ACGCTGTAACAGTAGACTACCAGACCCTGGAAGACGGCACAGTAACTGTGAGAGATAGAGATAGCTCTCTCCAGGAGAGA
GTAAAGATCGATGAGCTCGAGAAATACCTGCTTATGAAATACGGGTTCCAGCCCGACCTACTTACTCCTTAA

Upstream 100 bases:

>100_bases
CTGATCCCTGTAGTAGTAGCCTCCAATTCATGTTCACCACCACTTAAACGAATATTTAAGGTGAACGCGTTATTTAAAAT
CCAAGTACAAGGGGATCGAG

Downstream 100 bases:

>100_bases
CCAGCATTCGTGTTTTTAAGGTTGAACACGGTATATCTCAATATAATGGTGATTGAATGGAGCAGTCTACTAGTGGAGGA
GAAAAGGAAACAGGTACACA

Product: glycyl-tRNA synthetase

Products: NA

Alternate protein names: Glycine--tRNA ligase; GlyRS [H]

Number of amino acids: Translated: 583; Mature: 582

Protein sequence:

>583_residues
MSNGEDIYGNIMELAKKRGIFWGSFDIYGGLAGFYDFGPVGVLLKRNLINLWLKTFVYSNDLVVEIETPVINPRIVFKAS
GHEESFMDPVVECLKCGRVYRADHLVKEAAGIDVEGLSPEEIHSVILRNNIKCPECGGDLGKPFYTLLLFKTEIGPYKGE
IGYLRPENAQGMFINFANILRVARNRLPLGIAQVGKVARNEISPRQGLLRLREFTIMELEFFFDPEEVEEASGYINSIAD
VKVRVLTSEARVRGVEEPVEYTIRELVEKQIVKTPWLVYWIGLGEVFARRLGIPHEKIRFIEKLPHERAHYSQQTFDQEV
YTEKYGWIEIAGYAYRTTYDLSRHIQYSKADLTFFKHYEKPVERIIPKAYPDPGKIKDLYGGEMGRIMERLAQKTPEEIY
RELAEKGYVEIEGLKMPSEAFIVKQEREKIHGEKIIPHVVEPSFGVERLLYIVLEYSYTVRDGKTVLKLPVHLAPYHVAV
FPLVTGKKQEHARIIEIARNIYRRLITRGFRVIYDDDGSIGRRYARVDEIGIPYAVTVDYQTLEDGTVTVRDRDSSLQER
VKIDELEKYLLMKYGFQPDLLTP

Sequences:

>Translated_583_residues
MSNGEDIYGNIMELAKKRGIFWGSFDIYGGLAGFYDFGPVGVLLKRNLINLWLKTFVYSNDLVVEIETPVINPRIVFKAS
GHEESFMDPVVECLKCGRVYRADHLVKEAAGIDVEGLSPEEIHSVILRNNIKCPECGGDLGKPFYTLLLFKTEIGPYKGE
IGYLRPENAQGMFINFANILRVARNRLPLGIAQVGKVARNEISPRQGLLRLREFTIMELEFFFDPEEVEEASGYINSIAD
VKVRVLTSEARVRGVEEPVEYTIRELVEKQIVKTPWLVYWIGLGEVFARRLGIPHEKIRFIEKLPHERAHYSQQTFDQEV
YTEKYGWIEIAGYAYRTTYDLSRHIQYSKADLTFFKHYEKPVERIIPKAYPDPGKIKDLYGGEMGRIMERLAQKTPEEIY
RELAEKGYVEIEGLKMPSEAFIVKQEREKIHGEKIIPHVVEPSFGVERLLYIVLEYSYTVRDGKTVLKLPVHLAPYHVAV
FPLVTGKKQEHARIIEIARNIYRRLITRGFRVIYDDDGSIGRRYARVDEIGIPYAVTVDYQTLEDGTVTVRDRDSSLQER
VKIDELEKYLLMKYGFQPDLLTP
>Mature_582_residues
SNGEDIYGNIMELAKKRGIFWGSFDIYGGLAGFYDFGPVGVLLKRNLINLWLKTFVYSNDLVVEIETPVINPRIVFKASG
HEESFMDPVVECLKCGRVYRADHLVKEAAGIDVEGLSPEEIHSVILRNNIKCPECGGDLGKPFYTLLLFKTEIGPYKGEI
GYLRPENAQGMFINFANILRVARNRLPLGIAQVGKVARNEISPRQGLLRLREFTIMELEFFFDPEEVEEASGYINSIADV
KVRVLTSEARVRGVEEPVEYTIRELVEKQIVKTPWLVYWIGLGEVFARRLGIPHEKIRFIEKLPHERAHYSQQTFDQEVY
TEKYGWIEIAGYAYRTTYDLSRHIQYSKADLTFFKHYEKPVERIIPKAYPDPGKIKDLYGGEMGRIMERLAQKTPEEIYR
ELAEKGYVEIEGLKMPSEAFIVKQEREKIHGEKIIPHVVEPSFGVERLLYIVLEYSYTVRDGKTVLKLPVHLAPYHVAVF
PLVTGKKQEHARIIEIARNIYRRLITRGFRVIYDDDGSIGRRYARVDEIGIPYAVTVDYQTLEDGTVTVRDRDSSLQERV
KIDELEKYLLMKYGFQPDLLTP

Specific function: Catalyzes the attachment of glycine to tRNA(Gly) [H]

COG id: COG0423

COG function: function code J; Glycyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI116805340, Length=589, Percent_Identity=35.9932088285229, Blast_Score=351, Evalue=9e-97,
Organism=Caenorhabditis elegans, GI32564541, Length=589, Percent_Identity=35.4838709677419, Blast_Score=342, Evalue=4e-94,
Organism=Caenorhabditis elegans, GI17554996, Length=589, Percent_Identity=35.4838709677419, Blast_Score=342, Evalue=4e-94,
Organism=Saccharomyces cerevisiae, GI6319597, Length=625, Percent_Identity=31.52, Blast_Score=302, Evalue=9e-83,
Organism=Saccharomyces cerevisiae, GI6325338, Length=597, Percent_Identity=31.4907872696817, Blast_Score=296, Evalue=6e-81,
Organism=Drosophila melanogaster, GI24664462, Length=583, Percent_Identity=35.1629502572899, Blast_Score=339, Evalue=4e-93,
Organism=Drosophila melanogaster, GI21357965, Length=583, Percent_Identity=35.1629502572899, Blast_Score=338, Evalue=5e-93,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR022960
- InterPro:   IPR002315 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 67223; Mature: 67092

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNGEDIYGNIMELAKKRGIFWGSFDIYGGLAGFYDFGPVGVLLKRNLINLWLKTFVYSN
CCCCCHHHHHHHHHHHHCCCEEECHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
DLVVEIETPVINPRIVFKASGHEESFMDPVVECLKCGRVYRADHLVKEAAGIDVEGLSPE
CEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHH
EIHSVILRNNIKCPECGGDLGKPFYTLLLFKTEIGPYKGEIGYLRPENAQGMFINFANIL
HHHHHHHHCCCCCCCCCCCCCCHHHHHEEEEECCCCCCCCCCCCCCCCCCEEEEEHHHHH
RVARNRLPLGIAQVGKVARNEISPRQGLLRLREFTIMELEFFFDPEEVEEASGYINSIAD
HHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHH
VKVRVLTSEARVRGVEEPVEYTIRELVEKQIVKTPWLVYWIGLGEVFARRLGIPHEKIRF
EEEEEEECHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHCCCHHHHHH
IEKLPHERAHYSQQTFDQEVYTEKYGWIEIAGYAYRTTYDLSRHIQYSKADLTFFKHYEK
HHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEEEHHHHHCCCCCHHHHHHHHHHHH
PVERIIPKAYPDPGKIKDLYGGEMGRIMERLAQKTPEEIYRELAEKGYVEIEGLKMPSEA
HHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCCHH
FIVKQEREKIHGEKIIPHVVEPSFGVERLLYIVLEYSYTVRDGKTVLKLPVHLAPYHVAV
HEEEHHHHHCCCCCCCCHHCCCCCCHHHHHHHHHHHCEEECCCCEEEEECCEECCCEEEE
FPLVTGKKQEHARIIEIARNIYRRLITRGFRVIYDDDGSIGRRYARVDEIGIPYAVTVDY
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEE
QTLEDGTVTVRDRDSSLQERVKIDELEKYLLMKYGFQPDLLTP
EECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SNGEDIYGNIMELAKKRGIFWGSFDIYGGLAGFYDFGPVGVLLKRNLINLWLKTFVYSN
CCCCHHHHHHHHHHHHCCCEEECHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
DLVVEIETPVINPRIVFKASGHEESFMDPVVECLKCGRVYRADHLVKEAAGIDVEGLSPE
CEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHH
EIHSVILRNNIKCPECGGDLGKPFYTLLLFKTEIGPYKGEIGYLRPENAQGMFINFANIL
HHHHHHHHCCCCCCCCCCCCCCHHHHHEEEEECCCCCCCCCCCCCCCCCCEEEEEHHHHH
RVARNRLPLGIAQVGKVARNEISPRQGLLRLREFTIMELEFFFDPEEVEEASGYINSIAD
HHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHH
VKVRVLTSEARVRGVEEPVEYTIRELVEKQIVKTPWLVYWIGLGEVFARRLGIPHEKIRF
EEEEEEECHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHCCCHHHHHH
IEKLPHERAHYSQQTFDQEVYTEKYGWIEIAGYAYRTTYDLSRHIQYSKADLTFFKHYEK
HHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEEEHHHHHCCCCCHHHHHHHHHHHH
PVERIIPKAYPDPGKIKDLYGGEMGRIMERLAQKTPEEIYRELAEKGYVEIEGLKMPSEA
HHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCEEEECCCCCCHH
FIVKQEREKIHGEKIIPHVVEPSFGVERLLYIVLEYSYTVRDGKTVLKLPVHLAPYHVAV
HEEEHHHHHCCCCCCCCHHCCCCCCHHHHHHHHHHHCEEECCCCEEEEECCEECCCEEEE
FPLVTGKKQEHARIIEIARNIYRRLITRGFRVIYDDDGSIGRRYARVDEIGIPYAVTVDY
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEE
QTLEDGTVTVRDRDSSLQERVKIDELEKYLLMKYGFQPDLLTP
EECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]