Click here to switch to the map view.

The map label for this gene is hflB [C]

Identifier: 218883334

GI number: 218883334

Start: 16878

End: 18230

Strand: Direct

Name: hflB [C]

Synonym: DKAM_0020

Alternate gene names: 218883334

Gene position: 16878-18230 (Clockwise)

Preceding gene: 218883331

Following gene: 218883335

Centisome position: 1.24

GC content: 46.56

Gene sequence:

>1353_bases
ATGAGTGGAATACGTGTTGAAGCACCTGTTCGTAGGAGGATAAGTGCGCACAGCCACATCACCGGGTTAGGTTTGGATGA
AAAAGGTAGGGCTAAAATAATCGGTGATGGTTTAGTAGGACAGACGGAGGCTAGGGAGGCTGCCGGCATAGTTGTCAAGA
TGATTAGGGAGGGGAGAATAGCTGGAAGAGGGATATTGTTGGTGGGGCCACCCGGCACAGGTAAGACAGCCATAGCTGTG
GCAATAGCCAGGGAGCTCGGTGAGGAAACACCATTTGTCATAATGAGTGGTAGCGAGATATATAGTACGGAAAAGAAAAA
GACCGAAGTACTCATGGAGGCCTTAAGGAAGTCGCTTGGAATAAAGCTCAGGGAGGTTAGAAAAGTCTATGAAGGCGTTG
TAAAGGAGTTGAAGATACGTAGAGCGAGACACCCCATGGTGCCCTATCTAACAGTCCCAGTAGAGGCAAGGGTGGTTCTA
GCCACGAAGGATGAAGAGTTGACTTTGACAGTGCCTGAGGAAGTAACCCAGCAGCTCCTCGAGATGGGTATACGTAAAGG
CGACGTGATATGGATTGATGCTGAAACAGGTAGGGTCCACAGGGTTGGACGCACCAGGGAGGTGGAGGGTGCTAGAACAT
ATGATGTTGAGACTAAGAGGATCGTTGAAATACCCAAGGGGCCTGTGAGGAAGGAAAAGGAAATAGTAAACGTGTTAACC
CTGTATGATCTTGACATGATATACGCTGCACAGAGAAGCGTGATCAGCTTCTTCGGGTTAACCTTTGAAAGAGAGATACC
TTCAGAAGTAAGGCGTCAAGTAGATGAAACAGTGAAGAAGTGGATCGATGAGAAAAAAGCTGAATTACTCCCCGGTGTAC
TCTTCATAGATGACGCACATATGCTAGATATAGAATCATTCAGCTTCCTGACAAGAGCTATGGAGAGCGAGTTCGCCCCG
ATACTTATCCTGGCAACCAATAGAGGCATTGCAAGGATACGGGGTACGGACATAGAGTCGCCTCATGGCATGCCATTGGA
CTTACTGGATAGATTACTAATAATACCTACACAGCCTTACACACCTGATGAGATCAGGGAGATCATCAAGATACGTGCCA
GCGAGGAAGAGATAGCTTTATCTAATGAGGCTCTAGAGAAACTTGTCGAAATAGGTGGTAAGACAAGTCTAAGATACGCT
GTGCAACTACTTGAGCCAGCTAGAATAATTGCGGAGGAAAGGGGATCAAGCAGGATAGAGGTCGAAGACGTGGAGAGAGC
TAGAAAGCTCTTTATAGATGTATCCATAAGTACCGAGTATCTAAAACAATATGAGAGACTCTTCATGAAGTAA

Upstream 100 bases:

>100_bases
TAAAAATCCCTCTTACATAATTATTTATACTATGATAATCCATACTGCTCCGTAGAGGTTTAACCAGCTTAAGTTAGATG
ATCAGCCAAGGTGGTATGGG

Downstream 100 bases:

>100_bases
ATGAACTGGGGATGATGAGTAATAAATGTATATTGGTGATATTAATAAGGCTAGAGAATGGTTGGAGAAAGCTTTTTCTA
AGACCCCCAACCACTTGGAT

Product: TATA binding protein (TBP)-interacting protein

Products: NA

Alternate protein names: TIP49 Domain Protein; TIP49-Like Protein; Tbp-Interacting Protein Tip; TIP49 Domain-Containing Protein; DNA Helicase; TBP-Interacting Proten; TIP49 C-Terminal Domain Family Protein; DNA Helicase TIP; TIP49-Like

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MSGIRVEAPVRRRISAHSHITGLGLDEKGRAKIIGDGLVGQTEAREAAGIVVKMIREGRIAGRGILLVGPPGTGKTAIAV
AIARELGEETPFVIMSGSEIYSTEKKKTEVLMEALRKSLGIKLREVRKVYEGVVKELKIRRARHPMVPYLTVPVEARVVL
ATKDEELTLTVPEEVTQQLLEMGIRKGDVIWIDAETGRVHRVGRTREVEGARTYDVETKRIVEIPKGPVRKEKEIVNVLT
LYDLDMIYAAQRSVISFFGLTFEREIPSEVRRQVDETVKKWIDEKKAELLPGVLFIDDAHMLDIESFSFLTRAMESEFAP
ILILATNRGIARIRGTDIESPHGMPLDLLDRLLIIPTQPYTPDEIREIIKIRASEEEIALSNEALEKLVEIGGKTSLRYA
VQLLEPARIIAEERGSSRIEVEDVERARKLFIDVSISTEYLKQYERLFMK

Sequences:

>Translated_450_residues
MSGIRVEAPVRRRISAHSHITGLGLDEKGRAKIIGDGLVGQTEAREAAGIVVKMIREGRIAGRGILLVGPPGTGKTAIAV
AIARELGEETPFVIMSGSEIYSTEKKKTEVLMEALRKSLGIKLREVRKVYEGVVKELKIRRARHPMVPYLTVPVEARVVL
ATKDEELTLTVPEEVTQQLLEMGIRKGDVIWIDAETGRVHRVGRTREVEGARTYDVETKRIVEIPKGPVRKEKEIVNVLT
LYDLDMIYAAQRSVISFFGLTFEREIPSEVRRQVDETVKKWIDEKKAELLPGVLFIDDAHMLDIESFSFLTRAMESEFAP
ILILATNRGIARIRGTDIESPHGMPLDLLDRLLIIPTQPYTPDEIREIIKIRASEEEIALSNEALEKLVEIGGKTSLRYA
VQLLEPARIIAEERGSSRIEVEDVERARKLFIDVSISTEYLKQYERLFMK
>Mature_449_residues
SGIRVEAPVRRRISAHSHITGLGLDEKGRAKIIGDGLVGQTEAREAAGIVVKMIREGRIAGRGILLVGPPGTGKTAIAVA
IARELGEETPFVIMSGSEIYSTEKKKTEVLMEALRKSLGIKLREVRKVYEGVVKELKIRRARHPMVPYLTVPVEARVVLA
TKDEELTLTVPEEVTQQLLEMGIRKGDVIWIDAETGRVHRVGRTREVEGARTYDVETKRIVEIPKGPVRKEKEIVNVLTL
YDLDMIYAAQRSVISFFGLTFEREIPSEVRRQVDETVKKWIDEKKAELLPGVLFIDDAHMLDIESFSFLTRAMESEFAPI
LILATNRGIARIRGTDIESPHGMPLDLLDRLLIIPTQPYTPDEIREIIKIRASEEEIALSNEALEKLVEIGGKTSLRYAV
QLLEPARIIAEERGSSRIEVEDVERARKLFIDVSISTEYLKQYERLFMK

Specific function: Seems To Act As An ATP-Dependent Zinc Metallopeptidase. Involved In The Degradation Of Sigma-32. Degrades Carboxy- Terminal-Tagged Cytoplasmic Proteins. These Proteins Are Tagged With An 11-Amino-Acid Nonpolar Destabilizing Tail VIa A Mechanism Involving

COG id: COG1224

COG function: function code K; DNA helicase TIP49, TBP-interacting protein

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4506753, Length=446, Percent_Identity=47.9820627802691, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI5730023, Length=440, Percent_Identity=45.6818181818182, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17542510, Length=456, Percent_Identity=42.1052631578947, Blast_Score=358, Evalue=3e-99,
Organism=Caenorhabditis elegans, GI17558290, Length=432, Percent_Identity=40.2777777777778, Blast_Score=308, Evalue=5e-84,
Organism=Saccharomyces cerevisiae, GI6325021, Length=442, Percent_Identity=44.1176470588235, Blast_Score=378, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6320396, Length=437, Percent_Identity=46.4530892448513, Blast_Score=372, Evalue=1e-104,
Organism=Drosophila melanogaster, GI17737635, Length=440, Percent_Identity=46.8181818181818, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI21358125, Length=452, Percent_Identity=46.9026548672566, Blast_Score=363, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.4.24.- [C]

Molecular weight: Translated: 50706; Mature: 50575

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGIRVEAPVRRRISAHSHITGLGLDEKGRAKIIGDGLVGQTEAREAAGIVVKMIREGRI
CCCCCCCCHHHHHHHHCCCEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
AGRGILLVGPPGTGKTAIAVAIARELGEETPFVIMSGSEIYSTEKKKTEVLMEALRKSLG
CCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCHHHCCHHHHHHHHHHHHHHHHC
IKLREVRKVYEGVVKELKIRRARHPMVPYLTVPVEARVVLATKDEELTLTVPEEVTQQLL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEEEEEECCCCEEEECCHHHHHHHH
EMGIRKGDVIWIDAETGRVHRVGRTREVEGARTYDVETKRIVEIPKGPVRKEKEIVNVLT
HHCCCCCCEEEEECCCCCEEECCCCCCCCCCEEECCCCHHEEECCCCCCCHHHHHHHHHH
LYDLDMIYAAQRSVISFFGLTFEREIPSEVRRQVDETVKKWIDEKKAELLPGVLFIDDAH
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCC
MLDIESFSFLTRAMESEFAPILILATNRGIARIRGTDIESPHGMPLDLLDRLLIIPTQPY
EEEHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCCCHHHHCCEEEECCCCC
TPDEIREIIKIRASEEEIALSNEALEKLVEIGGKTSLRYAVQLLEPARIIAEERGSSRIE
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
VEDVERARKLFIDVSISTEYLKQYERLFMK
HHHHHHHHHEEEEEECCHHHHHHHHHHHCC
>Mature Secondary Structure 
SGIRVEAPVRRRISAHSHITGLGLDEKGRAKIIGDGLVGQTEAREAAGIVVKMIREGRI
CCCCCCCHHHHHHHHCCCEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
AGRGILLVGPPGTGKTAIAVAIARELGEETPFVIMSGSEIYSTEKKKTEVLMEALRKSLG
CCCEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCHHHCCHHHHHHHHHHHHHHHHC
IKLREVRKVYEGVVKELKIRRARHPMVPYLTVPVEARVVLATKDEELTLTVPEEVTQQLL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEEEEEECCCCEEEECCHHHHHHHH
EMGIRKGDVIWIDAETGRVHRVGRTREVEGARTYDVETKRIVEIPKGPVRKEKEIVNVLT
HHCCCCCCEEEEECCCCCEEECCCCCCCCCCEEECCCCHHEEECCCCCCCHHHHHHHHHH
LYDLDMIYAAQRSVISFFGLTFEREIPSEVRRQVDETVKKWIDEKKAELLPGVLFIDDAH
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCC
MLDIESFSFLTRAMESEFAPILILATNRGIARIRGTDIESPHGMPLDLLDRLLIIPTQPY
EEEHHHHHHHHHHHHCCCCCEEEEEECCCEEEEECCCCCCCCCCCHHHHCCEEEECCCCC
TPDEIREIIKIRASEEEIALSNEALEKLVEIGGKTSLRYAVQLLEPARIIAEERGSSRIE
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
VEDVERARKLFIDVSISTEYLKQYERLFMK
HHHHHHHHHEEEEEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Metalloendopeptidases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA