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The map label for this gene is kefA [H]

Identifier: 218533174

GI number: 218533174

Start: 5676393

End: 5679122

Strand: Reverse

Name: kefA [H]

Synonym: Mchl_5300

Alternate gene names: 218533174

Gene position: 5679122-5676393 (Counterclockwise)

Preceding gene: 218533176

Following gene: 218533173

Centisome position: 98.29

GC content: 68.61

Gene sequence:

>2730_bases
ATGCTACGACACTCCCTTCTCACCCGTGTCCGCCACATGATGCGCAGCAAGGCCCTGATCGCGGCACTGACCGCGTCGGC
TCTGCTGCCGAATGCGGCCGTGGGGCAGGGGACTCCGCCCGGCGCGCCGGCGCCGGCCGCTCAGTCGGCACCGGCCCTGA
AGCACCAGACGGCGGCGCCGCCGGCGAACGGGCAACCGCCTGCGGCAGACGGGCAGGCCGCGCCGGCTCCCAAGCCGGCC
GCGCCCAAGGTGCCCGTCTCGGAGGCGCTGCAAACGCTCCGCGACCGGCTCGACGCCATCAAGGCGGATCTCGAATCGCG
GGAAAAGGCGATTTCCGGACAGAATGTCGGGGCAGGCGACCTGACCCGCGCTCGCGACGGGCTCGACCCGCTCGCCGATC
GGCTGCGGTCGGTGATCGAGCTGCTCGGGCCGCGGCTGGAAGCCGCGCGCGAGCGGCTGACGCAAATCGGCCCGAAGCCG
AAGGAGGGCGAGGAGGGCGAGGAGGTCGCGCGTGAGCGGGCCGAGCGCGAGCAGGCGGTCAACGACATCGACGGCACCCA
GCGGCTGGCCAAGTCGCTGCTGGTGCAGAGCGACCAGATCGTCGATCAAATCTCGAATCGCCGTCGCGCCGCCTTCACCC
GCGGCCTGTTCGAGCGCAGCTCCTCGCTGTTGAGCCCCGACCTGTGGATGCGGGTCGCTGCCGACATTCCACGGGACCTC
AGCTCGCTGCGCAGCGGCGTCGACGACACCGTCTCTCTGTTCCGCCGCAACGGCACCCTGTGGAACCTGCTGGTTCTCGG
CCTCGCCTTCGGTCTGTCCTTCGCCCTCTATTTCGGCCGCCGCAACATCGCCCCGGCGCTCGGCCGCCGGGACATCAACG
TCACCACGCCGTCGAAGCGTGCCAAGCTTCTGGGTGCGTGGCGCGTGCTGCTTCTCGGCACGCTGCCGGCGCTGGCGGGC
AGCTACGCGGTCTATTACGCCCTGGACGTCACCGAGTTGCTGCCCGCCCGGTTCCTGCCGGTCGCCAGCATCATTCTCGG
CGGCATCGCCTTCATCGCCTTCGTCGAGGCGACCGCCGACGCGCTGCTCGCCGTCAACAAGCCGGCTTGGCGTCCGGCGC
CGGTCTCGGACGCCGCGGCATGGCGGATCAACGCGCTTGCCGTCAGCATCGCCGTGGTCATCACGGTGTCGAAATCGACC
GAGGCGCTGAACTCGGCGATCTACGCCGCGCTGCCGATTTCCATCGCCACCCGCGGCATCGGGGCCGTCACGGCCGCGCT
GCTGCTGGCCATCGGCCTCCATCGCTTCGCCGACACCGCGGAGAAGGAGGAGGAGTGCTTCGGCCCCTATGTCGGCACCG
ATACCTCGGCCAATATCGGCGGGCCGCTGCGGTTGCTCGGCTGGGTCGCGGTCGCGGTGATCGCGGTGGCCCCCTTCGTG
GGCTACGTCGCCCTCTCGGCCTTCCTCGTCGATCAGCTGATCTGGAGCGCGAGCGTCCTCGTCCTGCTCTGGCTCCTCAT
CATGAGCGTCGATGTGCTGATCGGCGGCTCGCTGTGCGAGGAGACCCGCCTCGCCACCACGCTCCAGGCCAATACCGGGC
TGCGCAAGCGCTCGCTCAACCAGATCGCGGTGCTGGTGACAGGTTTCGGCCGGGTCGTGCTGATCGCCGTGGCGGCGCTC
CTGACGCTCGCGCCCTGGGGGCTCGATTCGACGGACGTGTTCTCCTCGATCCGCACGGCCTTCTTCGGCTTCAAGGTCGG
CGACGTCACGATCTCGCTGTCCTCCATCGCCTACGGCATCGGCATCCTCGGGGTCGGCGTGTTCATCACCCGCGGCGTGC
AGCGCTGGCTGGAGAACACCTACCTTCCGGCGACCGACCTCGATGCCGGCCTGCGCAACTCGATCTCGACGGTCGCGGGC
TATGTCGGCTTCCTGGTCGCGCTGGCGCTGTCCTTCTCGTATCTGGGCCTCAGCCTCGAGAAGCTCACCATCGTCGCCGG
TGCGCTCTCCGTCGGTATCGGTTTCGGTCTTCAGTCGATCGTCAACAACTTCGTCTCGGGCCTGCTGCTGCTCTGGGAGC
GTCCGATCCGGGTCGGCGATCAGGTGCTGATCGGCGACAGCGAGGGCATCGTGAAGCGGATCTCGGTGCGCTCCACCGAA
ATCCAGACCTTCGACCGCTCGGCCGTGATCGTGCCGAACTCGAACCTGATCTCCGGCATCGTGAAGAACCGCGTGCGCGG
CGACCGAACCGGGCGAGTCATCCTCTCCGTCAGCGTCCTGCGCAGCAAGGATCCGGTCCAGGCCGCCGAGATGATGGTCG
CCTGCGCCAAGGCCCATCCGGACGTGCTCAAGGAGCCGCCGCCGCGGGTGGTGTTCAAGAAGATCGGCGATCCCTTCCTC
GAGTTCGAGCTGGTCGCCATGGTGGTCGACGTCAACCTCGGCCAGAAGGTGCAGAGCGACCTCAATTTCTCGGTGTTCAA
GACCCTGGCGGATGCCGAGTTCATCCCGCCGATGGGCCCGGCTTCGAGCTTCATCACCGTCCAGGGTCTGGAACCGGTCC
GCGACGCGCTCGGACAGATCGCTCACGCGGTCGGCAGCTCGGCCACGCAGGTGATCGCGCCCGCGGCGTCCGGAGCGGAC
GATGCCAGGAAGAGTTCGGCGCCGGAGGAAACTGCTCCCCGCAGCCCGGCGAACGAATCGCAGGATGTGAACCGTCGGCG
CCACGGATAG

Upstream 100 bases:

>100_bases
CAGACGGCTGTGGTCGCGCGACACCATCCGCGAACCGGCCGGCCAGGCTGCCCTGGCGCCGATCACCCGACGCGATCCCG
TTCCCCCGCGAAGGACGACG

Downstream 100 bases:

>100_bases
GCACGAGCTTCCTTCGGAAAAAGAAGCGCCGGCCCTGAGGATATCGGGGCCGGCGGCACCGATACACTTGAGTTTACCGG
GCGCAGGGGCCACCTCATTC

Product: MscS Mechanosensitive ion channel

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 909; Mature: 909

Protein sequence:

>909_residues
MLRHSLLTRVRHMMRSKALIAALTASALLPNAAVGQGTPPGAPAPAAQSAPALKHQTAAPPANGQPPAADGQAAPAPKPA
APKVPVSEALQTLRDRLDAIKADLESREKAISGQNVGAGDLTRARDGLDPLADRLRSVIELLGPRLEAARERLTQIGPKP
KEGEEGEEVARERAEREQAVNDIDGTQRLAKSLLVQSDQIVDQISNRRRAAFTRGLFERSSSLLSPDLWMRVAADIPRDL
SSLRSGVDDTVSLFRRNGTLWNLLVLGLAFGLSFALYFGRRNIAPALGRRDINVTTPSKRAKLLGAWRVLLLGTLPALAG
SYAVYYALDVTELLPARFLPVASIILGGIAFIAFVEATADALLAVNKPAWRPAPVSDAAAWRINALAVSIAVVITVSKST
EALNSAIYAALPISIATRGIGAVTAALLLAIGLHRFADTAEKEEECFGPYVGTDTSANIGGPLRLLGWVAVAVIAVAPFV
GYVALSAFLVDQLIWSASVLVLLWLLIMSVDVLIGGSLCEETRLATTLQANTGLRKRSLNQIAVLVTGFGRVVLIAVAAL
LTLAPWGLDSTDVFSSIRTAFFGFKVGDVTISLSSIAYGIGILGVGVFITRGVQRWLENTYLPATDLDAGLRNSISTVAG
YVGFLVALALSFSYLGLSLEKLTIVAGALSVGIGFGLQSIVNNFVSGLLLLWERPIRVGDQVLIGDSEGIVKRISVRSTE
IQTFDRSAVIVPNSNLISGIVKNRVRGDRTGRVILSVSVLRSKDPVQAAEMMVACAKAHPDVLKEPPPRVVFKKIGDPFL
EFELVAMVVDVNLGQKVQSDLNFSVFKTLADAEFIPPMGPASSFITVQGLEPVRDALGQIAHAVGSSATQVIAPAASGAD
DARKSSAPEETAPRSPANESQDVNRRRHG

Sequences:

>Translated_909_residues
MLRHSLLTRVRHMMRSKALIAALTASALLPNAAVGQGTPPGAPAPAAQSAPALKHQTAAPPANGQPPAADGQAAPAPKPA
APKVPVSEALQTLRDRLDAIKADLESREKAISGQNVGAGDLTRARDGLDPLADRLRSVIELLGPRLEAARERLTQIGPKP
KEGEEGEEVARERAEREQAVNDIDGTQRLAKSLLVQSDQIVDQISNRRRAAFTRGLFERSSSLLSPDLWMRVAADIPRDL
SSLRSGVDDTVSLFRRNGTLWNLLVLGLAFGLSFALYFGRRNIAPALGRRDINVTTPSKRAKLLGAWRVLLLGTLPALAG
SYAVYYALDVTELLPARFLPVASIILGGIAFIAFVEATADALLAVNKPAWRPAPVSDAAAWRINALAVSIAVVITVSKST
EALNSAIYAALPISIATRGIGAVTAALLLAIGLHRFADTAEKEEECFGPYVGTDTSANIGGPLRLLGWVAVAVIAVAPFV
GYVALSAFLVDQLIWSASVLVLLWLLIMSVDVLIGGSLCEETRLATTLQANTGLRKRSLNQIAVLVTGFGRVVLIAVAAL
LTLAPWGLDSTDVFSSIRTAFFGFKVGDVTISLSSIAYGIGILGVGVFITRGVQRWLENTYLPATDLDAGLRNSISTVAG
YVGFLVALALSFSYLGLSLEKLTIVAGALSVGIGFGLQSIVNNFVSGLLLLWERPIRVGDQVLIGDSEGIVKRISVRSTE
IQTFDRSAVIVPNSNLISGIVKNRVRGDRTGRVILSVSVLRSKDPVQAAEMMVACAKAHPDVLKEPPPRVVFKKIGDPFL
EFELVAMVVDVNLGQKVQSDLNFSVFKTLADAEFIPPMGPASSFITVQGLEPVRDALGQIAHAVGSSATQVIAPAASGAD
DARKSSAPEETAPRSPANESQDVNRRRHG
>Mature_909_residues
MLRHSLLTRVRHMMRSKALIAALTASALLPNAAVGQGTPPGAPAPAAQSAPALKHQTAAPPANGQPPAADGQAAPAPKPA
APKVPVSEALQTLRDRLDAIKADLESREKAISGQNVGAGDLTRARDGLDPLADRLRSVIELLGPRLEAARERLTQIGPKP
KEGEEGEEVARERAEREQAVNDIDGTQRLAKSLLVQSDQIVDQISNRRRAAFTRGLFERSSSLLSPDLWMRVAADIPRDL
SSLRSGVDDTVSLFRRNGTLWNLLVLGLAFGLSFALYFGRRNIAPALGRRDINVTTPSKRAKLLGAWRVLLLGTLPALAG
SYAVYYALDVTELLPARFLPVASIILGGIAFIAFVEATADALLAVNKPAWRPAPVSDAAAWRINALAVSIAVVITVSKST
EALNSAIYAALPISIATRGIGAVTAALLLAIGLHRFADTAEKEEECFGPYVGTDTSANIGGPLRLLGWVAVAVIAVAPFV
GYVALSAFLVDQLIWSASVLVLLWLLIMSVDVLIGGSLCEETRLATTLQANTGLRKRSLNQIAVLVTGFGRVVLIAVAAL
LTLAPWGLDSTDVFSSIRTAFFGFKVGDVTISLSSIAYGIGILGVGVFITRGVQRWLENTYLPATDLDAGLRNSISTVAG
YVGFLVALALSFSYLGLSLEKLTIVAGALSVGIGFGLQSIVNNFVSGLLLLWERPIRVGDQVLIGDSEGIVKRISVRSTE
IQTFDRSAVIVPNSNLISGIVKNRVRGDRTGRVILSVSVLRSKDPVQAAEMMVACAKAHPDVLKEPPPRVVFKKIGDPFL
EFELVAMVVDVNLGQKVQSDLNFSVFKTLADAEFIPPMGPASSFITVQGLEPVRDALGQIAHAVGSSATQVIAPAASGAD
DARKSSAPEETAPRSPANESQDVNRRRHG

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=284, Percent_Identity=32.0422535211268, Blast_Score=142, Evalue=9e-35,
Organism=Escherichia coli, GI2367355, Length=269, Percent_Identity=33.0855018587361, Blast_Score=126, Evalue=7e-30,
Organism=Escherichia coli, GI1787591, Length=159, Percent_Identity=32.0754716981132, Blast_Score=66, Evalue=1e-11,
Organism=Escherichia coli, GI1789291, Length=208, Percent_Identity=24.0384615384615, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 96400; Mature: 96400

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS01246 UPF0003

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRHSLLTRVRHMMRSKALIAALTASALLPNAAVGQGTPPGAPAPAAQSAPALKHQTAAP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCC
PANGQPPAADGQAAPAPKPAAPKVPVSEALQTLRDRLDAIKADLESREKAISGQNVGAGD
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LTRARDGLDPLADRLRSVIELLGPRLEAARERLTQIGPKPKEGEEGEEVARERAEREQAV
HHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
NDIDGTQRLAKSLLVQSDQIVDQISNRRRAAFTRGLFERSSSLLSPDLWMRVAADIPRDL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHH
SSLRSGVDDTVSLFRRNGTLWNLLVLGLAFGLSFALYFGRRNIAPALGRRDINVTTPSKR
HHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHHH
AKLLGAWRVLLLGTLPALAGSYAVYYALDVTELLPARFLPVASIILGGIAFIAFVEATAD
HHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLAVNKPAWRPAPVSDAAAWRINALAVSIAVVITVSKSTEALNSAIYAALPISIATRGI
HHHEECCCCCCCCCCCCHHHHHHHHHHEEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCH
GAVTAALLLAIGLHRFADTAEKEEECFGPYVGTDTSANIGGPLRLLGWVAVAVIAVAPFV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GYVALSAFLVDQLIWSASVLVLLWLLIMSVDVLIGGSLCEETRLATTLQANTGLRKRSLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHH
QIAVLVTGFGRVVLIAVAALLTLAPWGLDSTDVFSSIRTAFFGFKVGDVTISLSSIAYGI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCEEECCEEEEHHHHHHHH
GILGVGVFITRGVQRWLENTYLPATDLDAGLRNSISTVAGYVGFLVALALSFSYLGLSLE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
KLTIVAGALSVGIGFGLQSIVNNFVSGLLLLWERPIRVGDQVLIGDSEGIVKRISVRSTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCHHH
IQTFDRSAVIVPNSNLISGIVKNRVRGDRTGRVILSVSVLRSKDPVQAAEMMVACAKAHP
HHCCCCCEEEECCCHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHCCC
DVLKEPPPRVVFKKIGDPFLEFELVAMVVDVNLGQKVQSDLNFSVFKTLADAEFIPPMGP
HHHCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHCCCCCCCCCC
ASSFITVQGLEPVRDALGQIAHAVGSSATQVIAPAASGADDARKSSAPEETAPRSPANES
CCCEEEEECCHHHHHHHHHHHHHHCCCCHHEEECCCCCCCHHHHCCCCCCCCCCCCCCCC
QDVNRRRHG
HHHHHHCCC
>Mature Secondary Structure
MLRHSLLTRVRHMMRSKALIAALTASALLPNAAVGQGTPPGAPAPAAQSAPALKHQTAAP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCC
PANGQPPAADGQAAPAPKPAAPKVPVSEALQTLRDRLDAIKADLESREKAISGQNVGAGD
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
LTRARDGLDPLADRLRSVIELLGPRLEAARERLTQIGPKPKEGEEGEEVARERAEREQAV
HHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
NDIDGTQRLAKSLLVQSDQIVDQISNRRRAAFTRGLFERSSSLLSPDLWMRVAADIPRDL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHH
SSLRSGVDDTVSLFRRNGTLWNLLVLGLAFGLSFALYFGRRNIAPALGRRDINVTTPSKR
HHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCHHH
AKLLGAWRVLLLGTLPALAGSYAVYYALDVTELLPARFLPVASIILGGIAFIAFVEATAD
HHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLAVNKPAWRPAPVSDAAAWRINALAVSIAVVITVSKSTEALNSAIYAALPISIATRGI
HHHEECCCCCCCCCCCCHHHHHHHHHHEEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCH
GAVTAALLLAIGLHRFADTAEKEEECFGPYVGTDTSANIGGPLRLLGWVAVAVIAVAPFV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
GYVALSAFLVDQLIWSASVLVLLWLLIMSVDVLIGGSLCEETRLATTLQANTGLRKRSLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHH
QIAVLVTGFGRVVLIAVAALLTLAPWGLDSTDVFSSIRTAFFGFKVGDVTISLSSIAYGI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCEEECCEEEEHHHHHHHH
GILGVGVFITRGVQRWLENTYLPATDLDAGLRNSISTVAGYVGFLVALALSFSYLGLSLE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
KLTIVAGALSVGIGFGLQSIVNNFVSGLLLLWERPIRVGDQVLIGDSEGIVKRISVRSTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCHHH
IQTFDRSAVIVPNSNLISGIVKNRVRGDRTGRVILSVSVLRSKDPVQAAEMMVACAKAHP
HHCCCCCEEEECCCHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHCCC
DVLKEPPPRVVFKKIGDPFLEFELVAMVVDVNLGQKVQSDLNFSVFKTLADAEFIPPMGP
HHHCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHCCCCCCCCCC
ASSFITVQGLEPVRDALGQIAHAVGSSATQVIAPAASGADDARKSSAPEETAPRSPANES
CCCEEEEECCHHHHHHHHHHHHHHCCCCHHEEECCCCCCCHHHHCCCCCCCCCCCCCCCC
QDVNRRRHG
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]