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The map label for this gene is dacD [H]

Identifier: 218533141

GI number: 218533141

Start: 5639808

End: 5641292

Strand: Reverse

Name: dacD [H]

Synonym: Mchl_5267

Alternate gene names: 218533141

Gene position: 5641292-5639808 (Counterclockwise)

Preceding gene: 218533145

Following gene: 218533138

Centisome position: 97.64

GC content: 70.84

Gene sequence:

>1485_bases
ATGCGTCGCGTAGAAGGCCGACCCACCACGGGGTCCCGCGCTGTCGCCGTGCTGCTCCTGGCGACGAGTTTTCTCGTCGC
AGTGGCCGACCCGGCCGAGGCAAAACGCCGAGGCCACGCCCGCAAGGCGGTGAGCGGGTACAACCCGCCCTACGCCGCCA
TGGTCGTGGACGTGAAGTCGGGCCGCACGCTGCACGCGGTCAACGAGGACGCGCTGCGCCACCCGGCCTCGATCACCAAG
GTGATGACCCTCTACATGCTGTTCGAGCAGCTGGAGAAGGGCCGCTACGACCTCGACTCGCCGCTCTCGATCTCGGCCAA
CGCCGCCGCCCAGGCGCCGTCGAAGCTGGGGTTGCGCCCCGGCTCGACCGTCACGGTGGAGGAGGCGATCAAGGCGCTCG
TCACCAAGTCGGCCAACGACGTGGCCTGCGCCATCGGCGAGAACATCGCCGGCTCCGAGCCGCGCTTCGCCGAGATGATG
ACGCGCAAGGCCAAGGCGCTCGGCATGAGCCGCACCCACTACGCCAACGCTTCGGGCCTGCCCGACCCCGACCAGATCAC
CACGGCCCACGACCTCACTCTGCTGGCCCGTGCGATCCAGGATCGCTTCCCGCGCTACTACCGCTACTTCCAGACCCGCT
CCTTCGCCTTCCGCGGCCGGACCATCGGCAACCACAACCGCCTGCTCGGCAATGTCGAGGGCGTCGACGGCATCAAGACC
GGCTACACCCGCGATTCCGGCTTCAACCTGATGACCGCGGCCAAGCTCGGCGACCGGCAGATCGTCGCGATCGTGCTCGG
CGGCAAATCGGGCGCGAGCCGCGACCGGATCATGGCCGACCTCGTCCGCGCCAATCTGCCCCGCGCCTTCGCCGGCAGCC
GTCAGACGGCGCCCGTGGTCGAGGTGGCCGAGCGCGGTCGCCCGGCAGTCGTCGCCGAAGCCGATTCCCGCACCCGCACG
GTCCTTGCCTCCGCGGACGACGAAGACATCGAGACGACGAGCTCCACCAGCAACGCCCCGCTCGACATCCGGCCGGCCAC
CACCACGCCGGGATCGCGCCGCACCACCCTCCAGGCGCTGCCGGGCGCTGCCCAGGCCTATGCCGGCTCGGCGGGTCAGG
CGCCTTTCCCCGGCACGGCCGGCAAGTCGACCGCCCGCCTGCCCGCCGTCGAGGGCGTCGCCTCCCGCGCCGAGGGCCCG
CCGAAGGATGCCCGCGGCGAAAGCGCCAAGCCGGTCACGCCGAGCCCCTGGGTGATCCAGCTCGGTGCCATGGACGACGA
GGACAAGGCCAAGTCGATCCTCGCCGACGCCCGCAGCCGCACCGGCATGCTGTCCAAGGCCGCGCCCTACACGGTGCGCG
TGACCCATGGCGGCACCACGCTGTTCCGCGCCCGCTTCTCGGGATTTGCCGAACAGAGCACCGCGCAGGATGCCTGCGCC
GCGCTGAAGAAGAACGGCTTCAACTGCTTCGCGACCCGGAGCTGA

Upstream 100 bases:

>100_bases
CATCCTACCGTCTTCGACAGAGATTGCGGACCCGATGGCGGCGTTCGTGAAGGGTGCCAGCCGGGCTTGAACGTCCGACT
GTCGAGGCGAAGGTTTCGAG

Downstream 100 bases:

>100_bases
TCGCCTCCAACTGCCATCCTCGGTGCGGCTGCCGGCGCGTTCGGCCGGCGACCGCCCGATGACAAGAGCCGCAGGCAAAG
CGCTTTCCGACGAAGTGGCC

Product: serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6B; PBP-6B [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MRRVEGRPTTGSRAVAVLLLATSFLVAVADPAEAKRRGHARKAVSGYNPPYAAMVVDVKSGRTLHAVNEDALRHPASITK
VMTLYMLFEQLEKGRYDLDSPLSISANAAAQAPSKLGLRPGSTVTVEEAIKALVTKSANDVACAIGENIAGSEPRFAEMM
TRKAKALGMSRTHYANASGLPDPDQITTAHDLTLLARAIQDRFPRYYRYFQTRSFAFRGRTIGNHNRLLGNVEGVDGIKT
GYTRDSGFNLMTAAKLGDRQIVAIVLGGKSGASRDRIMADLVRANLPRAFAGSRQTAPVVEVAERGRPAVVAEADSRTRT
VLASADDEDIETTSSTSNAPLDIRPATTTPGSRRTTLQALPGAAQAYAGSAGQAPFPGTAGKSTARLPAVEGVASRAEGP
PKDARGESAKPVTPSPWVIQLGAMDDEDKAKSILADARSRTGMLSKAAPYTVRVTHGGTTLFRARFSGFAEQSTAQDACA
ALKKNGFNCFATRS

Sequences:

>Translated_494_residues
MRRVEGRPTTGSRAVAVLLLATSFLVAVADPAEAKRRGHARKAVSGYNPPYAAMVVDVKSGRTLHAVNEDALRHPASITK
VMTLYMLFEQLEKGRYDLDSPLSISANAAAQAPSKLGLRPGSTVTVEEAIKALVTKSANDVACAIGENIAGSEPRFAEMM
TRKAKALGMSRTHYANASGLPDPDQITTAHDLTLLARAIQDRFPRYYRYFQTRSFAFRGRTIGNHNRLLGNVEGVDGIKT
GYTRDSGFNLMTAAKLGDRQIVAIVLGGKSGASRDRIMADLVRANLPRAFAGSRQTAPVVEVAERGRPAVVAEADSRTRT
VLASADDEDIETTSSTSNAPLDIRPATTTPGSRRTTLQALPGAAQAYAGSAGQAPFPGTAGKSTARLPAVEGVASRAEGP
PKDARGESAKPVTPSPWVIQLGAMDDEDKAKSILADARSRTGMLSKAAPYTVRVTHGGTTLFRARFSGFAEQSTAQDACA
ALKKNGFNCFATRS
>Mature_494_residues
MRRVEGRPTTGSRAVAVLLLATSFLVAVADPAEAKRRGHARKAVSGYNPPYAAMVVDVKSGRTLHAVNEDALRHPASITK
VMTLYMLFEQLEKGRYDLDSPLSISANAAAQAPSKLGLRPGSTVTVEEAIKALVTKSANDVACAIGENIAGSEPRFAEMM
TRKAKALGMSRTHYANASGLPDPDQITTAHDLTLLARAIQDRFPRYYRYFQTRSFAFRGRTIGNHNRLLGNVEGVDGIKT
GYTRDSGFNLMTAAKLGDRQIVAIVLGGKSGASRDRIMADLVRANLPRAFAGSRQTAPVVEVAERGRPAVVAEADSRTRT
VLASADDEDIETTSSTSNAPLDIRPATTTPGSRRTTLQALPGAAQAYAGSAGQAPFPGTAGKSTARLPAVEGVASRAEGP
PKDARGESAKPVTPSPWVIQLGAMDDEDKAKSILADARSRTGMLSKAAPYTVRVTHGGTTLFRARFSGFAEQSTAQDACA
ALKKNGFNCFATRS

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Note=N-terminal lies in the periplasmic space (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=263, Percent_Identity=32.319391634981, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1787062, Length=233, Percent_Identity=33.9055793991416, Blast_Score=135, Evalue=9e-33,
Organism=Escherichia coli, GI1786851, Length=229, Percent_Identity=31.4410480349345, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI87082054, Length=226, Percent_Identity=25.6637168141593, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 52388; Mature: 52388

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRVEGRPTTGSRAVAVLLLATSFLVAVADPAEAKRRGHARKAVSGYNPPYAAMVVDVKS
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHHHHCCCCCCEEEEEEEECC
GRTLHAVNEDALRHPASITKVMTLYMLFEQLEKGRYDLDSPLSISANAAAQAPSKLGLRP
CCEEEECCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHCCCHHHCCCC
GSTVTVEEAIKALVTKSANDVACAIGENIAGSEPRFAEMMTRKAKALGMSRTHYANASGL
CCEEEHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
PDPDQITTAHDLTLLARAIQDRFPRYYRYFQTRSFAFRGRTIGNHNRLLGNVEGVDGIKT
CCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEECCCCCCCCCC
GYTRDSGFNLMTAAKLGDRQIVAIVLGGKSGASRDRIMADLVRANLPRAFAGSRQTAPVV
CCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCHHHCCCCCCCHHH
EVAERGRPAVVAEADSRTRTVLASADDEDIETTSSTSNAPLDIRPATTTPGSRRTTLQAL
HHHHCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHC
PGAAQAYAGSAGQAPFPGTAGKSTARLPAVEGVASRAEGPPKDARGESAKPVTPSPWVIQ
CCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEE
LGAMDDEDKAKSILADARSRTGMLSKAAPYTVRVTHGGTTLFRARFSGFAEQSTAQDACA
ECCCCCHHHHHHHHHHHHHHCCCHHCCCCEEEEEECCCCHHHHHHHHCHHHHHHHHHHHH
ALKKNGFNCFATRS
HHHHCCCEEEEECC
>Mature Secondary Structure
MRRVEGRPTTGSRAVAVLLLATSFLVAVADPAEAKRRGHARKAVSGYNPPYAAMVVDVKS
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHHHHCCCCCCEEEEEEEECC
GRTLHAVNEDALRHPASITKVMTLYMLFEQLEKGRYDLDSPLSISANAAAQAPSKLGLRP
CCEEEECCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHCCCHHHCCCC
GSTVTVEEAIKALVTKSANDVACAIGENIAGSEPRFAEMMTRKAKALGMSRTHYANASGL
CCEEEHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
PDPDQITTAHDLTLLARAIQDRFPRYYRYFQTRSFAFRGRTIGNHNRLLGNVEGVDGIKT
CCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEECCCCCCCCCC
GYTRDSGFNLMTAAKLGDRQIVAIVLGGKSGASRDRIMADLVRANLPRAFAGSRQTAPVV
CCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCHHHCCCCCCCHHH
EVAERGRPAVVAEADSRTRTVLASADDEDIETTSSTSNAPLDIRPATTTPGSRRTTLQAL
HHHHCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHC
PGAAQAYAGSAGQAPFPGTAGKSTARLPAVEGVASRAEGPPKDARGESAKPVTPSPWVIQ
CCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEE
LGAMDDEDKAKSILADARSRTGMLSKAAPYTVRVTHGGTTLFRARFSGFAEQSTAQDACA
ECCCCCHHHHHHHHHHHHHHCCCHHCCCCEEEEEECCCCHHHHHHHHCHHHHHHHHHHHH
ALKKNGFNCFATRS
HHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7737516; 11677609; 7751291 [H]