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The map label for this gene is gltX2 [H]

Identifier: 218533094

GI number: 218533094

Start: 5586678

End: 5588027

Strand: Reverse

Name: gltX2 [H]

Synonym: Mchl_5220

Alternate gene names: 218533094

Gene position: 5588027-5586678 (Counterclockwise)

Preceding gene: 218533095

Following gene: 218533088

Centisome position: 96.71

GC content: 72.96

Gene sequence:

>1350_bases
ATGGCCGCTTCCCCCCTCGTCCGCTTCGCTCCCTCGCCGACCGGCTTCCTGCATATCGGCAATGCCCGGCCGGCGCTGCT
GAACGCGTTGTTCGCGCGCCGCGAGGGCGGACGCTTCCTGCTGCGCCTCGACGACACCGACCGCGAGCGCTCGACCGAAG
AGTTCGCCACCGCCGCGGCCGAGGATCTCGGCTGGCTCGGCATCAAGCCGGACCTGTTCTTCCGCCAGAGCGATCGCACC
GCCCTCTACGACACGGCGGCCGAGCGGCTGAAGGCGGCCGGACGGCTCTATCCCTGCTACGAGACACCGGAGGAGCTGGA
TCGCCGCCGCAAGCGCCAGCTCGGCCGCGGCCTGCCGCCGATCTACGACCGCGCGGCGCTCGCGCTGACCGAGGCCGAGC
GCGCCGCGTTCGAGGCCGAGGGCCGGAAGCCGCATTGGCGGTTCAAGCTCGACCACCGCGTCGTTGCCTGGAACGACCTC
GTGCGGGGCGAGAGCCACGTCGATTGCGCCTCGCTCTCCGACCCCGTGCTGGTGCGTGCCGACGGGAGCTACCTCTACAC
GCTGCCTTCGGTGGTGGACGACGCGGAGGTCGGCGTGACCCACGTGATCCGCGGCGAGGATCACGTGACGAATACCGGCG
TGCAGGTGCAGCTCTTCGAAGCGCTCGCCGCCTCCGTGCCCGCCTTCGGCCACCACAACCTGCTGACGACCGCGGACGGG
GAGGGGCTGTCGAAGCGCCTCGGCCACCTGTCTCTGCGCTCGCTGCGCGAGGCCGGCTACGAACCCGCCGCCGTGCGCTC
ACTGGCGGTGCTGACCGGCTCGGCCGAGGCCGTGCGCCCGATCGCCTCGCTCGACGAACTGGCTGATCTCGTCGACCTCG
CCCACCTCTCCCGCGCCCCGGCCCGGTTCGACCCGGCCGAACTCGACGGGATGAACGCGCGCCTCGTCCACGACATGCCG
CTCGAGGACGTGCGCGAGCGGCTTTCCGCCCTCGGTGTCCCCGCCGAGGCGGCGGAAGCCTTCTGGCTGGCGGTGCGGGC
CAATCTCGGGCGCGTGTCGGACGCCGCCGCATGGTGGCAGGTGGTGAACGGGCCGGTGACGCCCGTGATCGCCGAGCCCG
ACTTTATCGCGAGGGCCGCCGACCTGCTGCCGGAGGCGCCGTTCGGCCCCGGTACGTGGAAGGCCTGGACCGACGCGGTG
AAGGCCGAGACCGGCGCCAAGGGCCGCGCCCTGTTCATGCCGCTGCGCCTCGCGCTTACCGGCCTCGACCACGGGCCGGA
CCTCGCGGCGCTGCTGCCGCTGATCGGGCGCGAGCGGGCCGTGCGGCGGCTCGCCGGCGAGACCGCCTAA

Upstream 100 bases:

>100_bases
GTCGTCCCGATCGGACCGAGCCCGGCGCGGCGCCGAAGAACTGAGGCCGCGACTGCCCGTGGACGGTGCGGCGTCTGCGA
AAATTCGGTAGAGCGGCTTC

Downstream 100 bases:

>100_bases
TCCGAAACCCCATCGGGTTCGGCCTCGATCATCATGACGTCGAGGGTGAAGCTGCTGTCGTCCTCCAGGCCGAAGTGGGA
TCGCACCGGCTCCGGCGCGC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2 [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MAASPLVRFAPSPTGFLHIGNARPALLNALFARREGGRFLLRLDDTDRERSTEEFATAAAEDLGWLGIKPDLFFRQSDRT
ALYDTAAERLKAAGRLYPCYETPEELDRRRKRQLGRGLPPIYDRAALALTEAERAAFEAEGRKPHWRFKLDHRVVAWNDL
VRGESHVDCASLSDPVLVRADGSYLYTLPSVVDDAEVGVTHVIRGEDHVTNTGVQVQLFEALAASVPAFGHHNLLTTADG
EGLSKRLGHLSLRSLREAGYEPAAVRSLAVLTGSAEAVRPIASLDELADLVDLAHLSRAPARFDPAELDGMNARLVHDMP
LEDVRERLSALGVPAEAAEAFWLAVRANLGRVSDAAAWWQVVNGPVTPVIAEPDFIARAADLLPEAPFGPGTWKAWTDAV
KAETGAKGRALFMPLRLALTGLDHGPDLAALLPLIGRERAVRRLAGETA

Sequences:

>Translated_449_residues
MAASPLVRFAPSPTGFLHIGNARPALLNALFARREGGRFLLRLDDTDRERSTEEFATAAAEDLGWLGIKPDLFFRQSDRT
ALYDTAAERLKAAGRLYPCYETPEELDRRRKRQLGRGLPPIYDRAALALTEAERAAFEAEGRKPHWRFKLDHRVVAWNDL
VRGESHVDCASLSDPVLVRADGSYLYTLPSVVDDAEVGVTHVIRGEDHVTNTGVQVQLFEALAASVPAFGHHNLLTTADG
EGLSKRLGHLSLRSLREAGYEPAAVRSLAVLTGSAEAVRPIASLDELADLVDLAHLSRAPARFDPAELDGMNARLVHDMP
LEDVRERLSALGVPAEAAEAFWLAVRANLGRVSDAAAWWQVVNGPVTPVIAEPDFIARAADLLPEAPFGPGTWKAWTDAV
KAETGAKGRALFMPLRLALTGLDHGPDLAALLPLIGRERAVRRLAGETA
>Mature_448_residues
AASPLVRFAPSPTGFLHIGNARPALLNALFARREGGRFLLRLDDTDRERSTEEFATAAAEDLGWLGIKPDLFFRQSDRTA
LYDTAAERLKAAGRLYPCYETPEELDRRRKRQLGRGLPPIYDRAALALTEAERAAFEAEGRKPHWRFKLDHRVVAWNDLV
RGESHVDCASLSDPVLVRADGSYLYTLPSVVDDAEVGVTHVIRGEDHVTNTGVQVQLFEALAASVPAFGHHNLLTTADGE
GLSKRLGHLSLRSLREAGYEPAAVRSLAVLTGSAEAVRPIASLDELADLVDLAHLSRAPARFDPAELDGMNARLVHDMPL
EDVRERLSALGVPAEAAEAFWLAVRANLGRVSDAAAWWQVVNGPVTPVIAEPDFIARAADLLPEAPFGPGTWKAWTDAVK
AETGAKGRALFMPLRLALTGLDHGPDLAALLPLIGRERAVRRLAGETA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=481, Percent_Identity=30.977130977131, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI62241042, Length=107, Percent_Identity=38.3177570093458, Blast_Score=76, Evalue=8e-14,
Organism=Escherichia coli, GI1788743, Length=471, Percent_Identity=30.5732484076433, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI226510925, Length=273, Percent_Identity=32.2344322344322, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71993394, Length=307, Percent_Identity=36.4820846905537, Blast_Score=169, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17511007, Length=207, Percent_Identity=27.536231884058, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324540, Length=479, Percent_Identity=29.4363256784969, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6321192, Length=217, Percent_Identity=32.7188940092166, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6324742, Length=112, Percent_Identity=34.8214285714286, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24665321, Length=477, Percent_Identity=30.8176100628931, Blast_Score=193, Evalue=3e-49,
Organism=Drosophila melanogaster, GI24649466, Length=206, Percent_Identity=29.6116504854369, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 48855; Mature: 48724

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASPLVRFAPSPTGFLHIGNARPALLNALFARREGGRFLLRLDDTDRERSTEEFATAAA
CCCCCCEEECCCCCCEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHH
EDLGWLGIKPDLFFRQSDRTALYDTAAERLKAAGRLYPCYETPEELDRRRKRQLGRGLPP
HHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCC
IYDRAALALTEAERAAFEAEGRKPHWRFKLDHRVVAWNDLVRGESHVDCASLSDPVLVRA
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEHHHHCCCCCCCCCCCCCCEEEEE
DGSYLYTLPSVVDDAEVGVTHVIRGEDHVTNTGVQVQLFEALAASVPAFGHHNLLTTADG
CCCEEEECCCHHCCCCCCEEEEECCCCCCCCCCCEEHHHHHHHHHCCCCCCCCEEEECCC
EGLSKRLGHLSLRSLREAGYEPAAVRSLAVLTGSAEAVRPIASLDELADLVDLAHLSRAP
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ARFDPAELDGMNARLVHDMPLEDVRERLSALGVPAEAAEAFWLAVRANLGRVSDAAAWWQ
CCCCCHHCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHEEHHCCCCCHHHHHHHH
VVNGPVTPVIAEPDFIARAADLLPEAPFGPGTWKAWTDAVKAETGAKGRALFMPLRLALT
HHCCCCCEEEECCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHH
GLDHGPDLAALLPLIGRERAVRRLAGETA
CCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AASPLVRFAPSPTGFLHIGNARPALLNALFARREGGRFLLRLDDTDRERSTEEFATAAA
CCCCCEEECCCCCCEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHH
EDLGWLGIKPDLFFRQSDRTALYDTAAERLKAAGRLYPCYETPEELDRRRKRQLGRGLPP
HHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCC
IYDRAALALTEAERAAFEAEGRKPHWRFKLDHRVVAWNDLVRGESHVDCASLSDPVLVRA
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEHHHHCCCCCCCCCCCCCCEEEEE
DGSYLYTLPSVVDDAEVGVTHVIRGEDHVTNTGVQVQLFEALAASVPAFGHHNLLTTADG
CCCEEEECCCHHCCCCCCEEEEECCCCCCCCCCCEEHHHHHHHHHCCCCCCCCEEEECCC
EGLSKRLGHLSLRSLREAGYEPAAVRSLAVLTGSAEAVRPIASLDELADLVDLAHLSRAP
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ARFDPAELDGMNARLVHDMPLEDVRERLSALGVPAEAAEAFWLAVRANLGRVSDAAAWWQ
CCCCCHHCCCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHEEHHCCCCCHHHHHHHH
VVNGPVTPVIAEPDFIARAADLLPEAPFGPGTWKAWTDAVKAETGAKGRALFMPLRLALT
HHCCCCCEEEECCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHH
GLDHGPDLAALLPLIGRERAVRRLAGETA
CCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA