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The map label for this gene is ccsA [H]

Identifier: 218533021

GI number: 218533021

Start: 5508635

End: 5509924

Strand: Reverse

Name: ccsA [H]

Synonym: Mchl_5145

Alternate gene names: 218533021

Gene position: 5509924-5508635 (Counterclockwise)

Preceding gene: 218533022

Following gene: 218533008

Centisome position: 95.36

GC content: 63.33

Gene sequence:

>1290_bases
ATGAGCGCTTCCGCCAGCACCATCATCGTGGGCGACAAAAACGTCGAATTGCCCATCAAGACTGGAACGATCGGCCCCGA
CGTGGTCGATATCGGCAAGCTCTACGCCCAGACCGGGAAGTTCACCTTCGATCCGGGCTTCACCTCGACCGCCTCCTGCG
AGTCGAAGATCACTTATATCGACGGCGACGAGGGCGTGCTGCTCTATCGCGGCTACCCGATCGAGCAGCTCGCCGAGAAC
GGCGACTTCCTTGAGACCGCCTACCTGATGCTGTTCGGCGAGCTGCCCAGCTCGGCCCAGAAGGCGGATTTCGACTACCG
GGTCACGCGCCACACCATGGTGCACGACCAGATGAACCGCTTCTTCCAGGGTTTCCGCCGCGACGCCCACCCGATGGCGG
TGATGGTGGCCTGCGTCGGCGCGCTCTCGGCCTTCTATCATGACTCGACCGACATCTCGGACGAGTCGCAGCGGATGATC
GCCTCGCTGCGCATGATCGCGAAGATGCCGACGCTGGCGGCGATGGCCTACAAGTACACGATCGGCCAGCCCTTCGTGTA
TCCGAAGAACGACCTCGACTACACCTCGAACTTCCTGCGGATGTGCTTCGCGGTTCCGTGCGAGGAATACGTCGTCAACC
CGATCTACGCCCGCGCGCTGGACAAGATCTTCATCCTGCACGCCGACCACGAGCAGAACGCCTCGACCTCGACGGTGCGT
CTCGCCGGCTCCTCGGGCGCCAACCCGTTTGCCTGCATCGCCGCCGGCATCGCCTGCCTGTGGGGCCCGGCCCATGGCGG
CGCCAACGAGGCGGCGCTCAAGATGCTCATGGAGATCGGGCATCCCGAGAACGTGCAGAAATACGTCGCCAAGGCGAAGG
ACAAGAACGATCCCTTCCGCCTGATGGGCTTCGGCCACCGGGTCTACAAGAACTACGACCCGCGTGCGCGCATCATGCAG
AAGACGACCCACGAGGTTCTGAACGAACTCGGGATCAAGGACGATTTGCTGGAGGTCGCCGTCCAGCTCGAGAAGATCGC
CCTCGAGGACGAGTACTTCATCGAGAAGAAGCTCTACCCGAACATCGACTTCTACTCGGGCATCACCCTCAAGGCGCTCG
GCTTCCCGACCTCGATGTTCACGGTGCTGTTCGCGCTCGCCCGCACCGTCGGCTGGATCGCGCAGTGGGCCGAGATGATC
GAGGACCCGTCCCAGAAGATCGGCCGCCCGCGCCAGCTCTATATCGGCCCGGATCGCCGCGACTACACGCCGATCGGCCA
GCGGAGCTGA

Upstream 100 bases:

>100_bases
GTGAGCGGGCGCAGTGCTCTGCTGCGGCGCCACAACGGGGCGGGAGGCGATGCCTTCCGGTCCAGTGCTGTTCCCGTCGC
AAGAGAGGGTCCGCGATCCC

Downstream 100 bases:

>100_bases
GCTGGAGCATCATCCCGAAAGGTGGCCTCCGGCTTTCGGGATGATGCAAAACAAAAGCCTAGAGCATCGTCTTGGGTCCG
ATATCCAGGACGATGCTCTA

Product: citrate synthase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSASASTIIVGDKNVELPIKTGTIGPDVVDIGKLYAQTGKFTFDPGFTSTASCESKITYIDGDEGVLLYRGYPIEQLAEN
GDFLETAYLMLFGELPSSAQKADFDYRVTRHTMVHDQMNRFFQGFRRDAHPMAVMVACVGALSAFYHDSTDISDESQRMI
ASLRMIAKMPTLAAMAYKYTIGQPFVYPKNDLDYTSNFLRMCFAVPCEEYVVNPIYARALDKIFILHADHEQNASTSTVR
LAGSSGANPFACIAAGIACLWGPAHGGANEAALKMLMEIGHPENVQKYVAKAKDKNDPFRLMGFGHRVYKNYDPRARIMQ
KTTHEVLNELGIKDDLLEVAVQLEKIALEDEYFIEKKLYPNIDFYSGITLKALGFPTSMFTVLFALARTVGWIAQWAEMI
EDPSQKIGRPRQLYIGPDRRDYTPIGQRS

Sequences:

>Translated_429_residues
MSASASTIIVGDKNVELPIKTGTIGPDVVDIGKLYAQTGKFTFDPGFTSTASCESKITYIDGDEGVLLYRGYPIEQLAEN
GDFLETAYLMLFGELPSSAQKADFDYRVTRHTMVHDQMNRFFQGFRRDAHPMAVMVACVGALSAFYHDSTDISDESQRMI
ASLRMIAKMPTLAAMAYKYTIGQPFVYPKNDLDYTSNFLRMCFAVPCEEYVVNPIYARALDKIFILHADHEQNASTSTVR
LAGSSGANPFACIAAGIACLWGPAHGGANEAALKMLMEIGHPENVQKYVAKAKDKNDPFRLMGFGHRVYKNYDPRARIMQ
KTTHEVLNELGIKDDLLEVAVQLEKIALEDEYFIEKKLYPNIDFYSGITLKALGFPTSMFTVLFALARTVGWIAQWAEMI
EDPSQKIGRPRQLYIGPDRRDYTPIGQRS
>Mature_428_residues
SASASTIIVGDKNVELPIKTGTIGPDVVDIGKLYAQTGKFTFDPGFTSTASCESKITYIDGDEGVLLYRGYPIEQLAENG
DFLETAYLMLFGELPSSAQKADFDYRVTRHTMVHDQMNRFFQGFRRDAHPMAVMVACVGALSAFYHDSTDISDESQRMIA
SLRMIAKMPTLAAMAYKYTIGQPFVYPKNDLDYTSNFLRMCFAVPCEEYVVNPIYARALDKIFILHADHEQNASTSTVRL
AGSSGANPFACIAAGIACLWGPAHGGANEAALKMLMEIGHPENVQKYVAKAKDKNDPFRLMGFGHRVYKNYDPRARIMQK
TTHEVLNELGIKDDLLEVAVQLEKIALEDEYFIEKKLYPNIDFYSGITLKALGFPTSMFTVLFALARTVGWIAQWAEMIE
DPSQKIGRPRQLYIGPDRRDYTPIGQRS

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=394, Percent_Identity=25.6345177664975, Blast_Score=99, Evalue=8e-21,
Organism=Escherichia coli, GI1786939, Length=418, Percent_Identity=67.2248803827751, Blast_Score=604, Evalue=1e-174,
Organism=Escherichia coli, GI1786527, Length=363, Percent_Identity=29.2011019283747, Blast_Score=162, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17555174, Length=400, Percent_Identity=27.75, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319850, Length=333, Percent_Identity=26.4264264264264, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324328, Length=332, Percent_Identity=25, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6325257, Length=408, Percent_Identity=25.4901960784314, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24640126, Length=413, Percent_Identity=26.1501210653753, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24640124, Length=401, Percent_Identity=26.1845386533666, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356863, Length=364, Percent_Identity=27.1978021978022, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 47944; Mature: 47813

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASASTIIVGDKNVELPIKTGTIGPDVVDIGKLYAQTGKFTFDPGFTSTASCESKITYI
CCCCCCEEEEECCCEEEEEECCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCEEEEE
DGDEGVLLYRGYPIEQLAENGDFLETAYLMLFGELPSSAQKADFDYRVTRHTMVHDQMNR
ECCCCEEEECCCCHHHHHCCCCHHHHHHHHHHHCCCCCCHHCCCCEEHHHHHHHHHHHHH
FFQGFRRDAHPMAVMVACVGALSAFYHDSTDISDESQRMIASLRMIAKMPTLAAMAYKYT
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHEE
IGQPFVYPKNDLDYTSNFLRMCFAVPCEEYVVNPIYARALDKIFILHADHEQNASTSTVR
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCHHHHHHHHEEEEEECCCCCCCCCCEEE
LAGSSGANPFACIAAGIACLWGPAHGGANEAALKMLMEIGHPENVQKYVAKAKDKNDPFR
EECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEE
LMGFGHRVYKNYDPRARIMQKTTHEVLNELGIKDDLLEVAVQLEKIALEDEYFIEKKLYP
EEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCC
NIDFYSGITLKALGFPTSMFTVLFALARTVGWIAQWAEMIEDPSQKIGRPRQLYIGPDRR
CCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEEEEECCCCC
DYTPIGQRS
CCCCCCCCC
>Mature Secondary Structure 
SASASTIIVGDKNVELPIKTGTIGPDVVDIGKLYAQTGKFTFDPGFTSTASCESKITYI
CCCCCEEEEECCCEEEEEECCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCEEEEE
DGDEGVLLYRGYPIEQLAENGDFLETAYLMLFGELPSSAQKADFDYRVTRHTMVHDQMNR
ECCCCEEEECCCCHHHHHCCCCHHHHHHHHHHHCCCCCCHHCCCCEEHHHHHHHHHHHHH
FFQGFRRDAHPMAVMVACVGALSAFYHDSTDISDESQRMIASLRMIAKMPTLAAMAYKYT
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHEE
IGQPFVYPKNDLDYTSNFLRMCFAVPCEEYVVNPIYARALDKIFILHADHEQNASTSTVR
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHCCHHHHHHHHEEEEEECCCCCCCCCCEEE
LAGSSGANPFACIAAGIACLWGPAHGGANEAALKMLMEIGHPENVQKYVAKAKDKNDPFR
EECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEE
LMGFGHRVYKNYDPRARIMQKTTHEVLNELGIKDDLLEVAVQLEKIALEDEYFIEKKLYP
EEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCC
NIDFYSGITLKALGFPTSMFTVLFALARTVGWIAQWAEMIEDPSQKIGRPRQLYIGPDRR
CCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEEEEECCCCC
DYTPIGQRS
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8526514 [H]