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The map label for this gene is gltX1 [H]

Identifier: 218533022

GI number: 218533022

Start: 5510400

End: 5511824

Strand: Reverse

Name: gltX1 [H]

Synonym: Mchl_5146

Alternate gene names: 218533022

Gene position: 5511824-5510400 (Counterclockwise)

Preceding gene: 218533024

Following gene: 218533021

Centisome position: 95.39

GC content: 71.58

Gene sequence:

>1425_bases
ATGTCGTCACCGGTCGTCACGCGCTTTGCGCCTTCACCCACAGGCTTCCTGCATATCGGGGGAGCGCGCACGGCTCTGTT
CAATTGGCTCTACGCCCGCCGCTTCGGCGGGCGGATGCTCTTGCGCATCGAGGACACCGACCGGGAGCGATCGACGCAAG
GGGCGATCGACGCGATCCTCGACGGGATGCGCTGGCTCGGCCTCGATTGGGACGGGGACGTGATCTACCAGTTCGCCCGC
GCCGAGCGGCACCGCGAGGTCGCCGAGAGCCTGCTCGCCTCGGGCAACGCCTATCACTGCTACGCCAGTGCGGAAGAGCT
GACGCAGATGCGCGAGACCGCCCGCGCCGAGGGCCGCGCCCCGCGCTACGACGGCCGCTGGCGCGACCGCGACCCGTCCG
AGGCGCCGGCGGGCGTGAAGCCGGTGATCCGCCTGCGCGCGCCGAGCGAGGGCGAGACGGTGGTCGAGGACGCCGTGCAG
GGCCGCGTCACCTGGGCCAACCGCGATCTCGACGACCTCGTGCTGCTGCGCTCCGACGGCACGCCGACCTACATGCTCGC
CGTCGTGGTGGACGACCACGACATGGGCGTCACGCAGGTGATCCGCGGCGACGACCACCTCACCAACGCCGCCCGCCAGA
GCCAGATCTACCGGGCGCTCGGCTGGGACGTGCCCGCCATGGCGCATATCCCGCTCATCCATGGAGCGGACGGCGCCAAG
CTGTCGAAGCGCCACGGCGCGCTCGGCGTCGAGGAATATCGCGACCGCGGCTACCTCCCGGCGGCCCTGCGCAACTACCT
CGTGCGGCTCGGCTGGAGCCATGGCGATCAGGAAGTCTTCTCCACCGAGGAGATGATCGCCGCCTTCGACCTGAAGGCGA
TCGGTCGCTCGGCTGCGCGGTTCGACTTCACGAAGCTGGAAAGCCTCAACGGGCTCTACATCCGCGGCAGCGCCGATTCC
GTGCTGGTGGATGCGATCGACGCGATCCTGCCGGCCCGCGGCCCCGAGCGCGGCCTGCCGACCGCGCTGACGCCGGATCT
GCGCGAGAAGCTCGTATCGGCGATGCCCGGCCTGAAGGAGCGCGCCAAGACGCTCGTCGAACTCCTCGACAGCGCCTACT
ACCTCTACGCCGCGCGTCCGCTCGCCCTCGACGCCAAGGCCGAGGCCCTGCTCGCGGGGGATGCGCCCGAGCGGCTGCGC
GCGCTCCTGCCGGCGCTCGAAGCCCTCCCGGAATGGAACGCGGCGACGACGGAGGCTGCGGTACGCCAATTCGCCGACAG
TCAGGGGGTCAAGCTCGGCCAGGTCGCCCAGCCCCTCCGGGCGGCGCTCACGGGGCGCGCCACCTCGCCGCCGGTCTTCG
ACGTCCTGGCCGTGCTCGGCCGCGACGAGGCGCTTGCCCGCCTCCGGGATCGCCTGCCGGCCTGA

Upstream 100 bases:

>100_bases
TTCTTAACGCATCGCCTCGCTCGTGCTACACGCCCGCCAAGACCGGCCGCAGCCGCACGCGCGCCATCCCGCACTTTTTG
AAGAAGCCTCCCAGCCGCCG

Downstream 100 bases:

>100_bases
GCCGGCCACGCGACGGATGCCCGCAGAGAAGATCCGGGTGCATCGGCGAAGCCGGTGCACCGCGTGCCGTTTCGGCGCGC
TGATGGGCTTCGCGGCGCCT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1 [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSSPVVTRFAPSPTGFLHIGGARTALFNWLYARRFGGRMLLRIEDTDRERSTQGAIDAILDGMRWLGLDWDGDVIYQFAR
AERHREVAESLLASGNAYHCYASAEELTQMRETARAEGRAPRYDGRWRDRDPSEAPAGVKPVIRLRAPSEGETVVEDAVQ
GRVTWANRDLDDLVLLRSDGTPTYMLAVVVDDHDMGVTQVIRGDDHLTNAARQSQIYRALGWDVPAMAHIPLIHGADGAK
LSKRHGALGVEEYRDRGYLPAALRNYLVRLGWSHGDQEVFSTEEMIAAFDLKAIGRSAARFDFTKLESLNGLYIRGSADS
VLVDAIDAILPARGPERGLPTALTPDLREKLVSAMPGLKERAKTLVELLDSAYYLYAARPLALDAKAEALLAGDAPERLR
ALLPALEALPEWNAATTEAAVRQFADSQGVKLGQVAQPLRAALTGRATSPPVFDVLAVLGRDEALARLRDRLPA

Sequences:

>Translated_474_residues
MSSPVVTRFAPSPTGFLHIGGARTALFNWLYARRFGGRMLLRIEDTDRERSTQGAIDAILDGMRWLGLDWDGDVIYQFAR
AERHREVAESLLASGNAYHCYASAEELTQMRETARAEGRAPRYDGRWRDRDPSEAPAGVKPVIRLRAPSEGETVVEDAVQ
GRVTWANRDLDDLVLLRSDGTPTYMLAVVVDDHDMGVTQVIRGDDHLTNAARQSQIYRALGWDVPAMAHIPLIHGADGAK
LSKRHGALGVEEYRDRGYLPAALRNYLVRLGWSHGDQEVFSTEEMIAAFDLKAIGRSAARFDFTKLESLNGLYIRGSADS
VLVDAIDAILPARGPERGLPTALTPDLREKLVSAMPGLKERAKTLVELLDSAYYLYAARPLALDAKAEALLAGDAPERLR
ALLPALEALPEWNAATTEAAVRQFADSQGVKLGQVAQPLRAALTGRATSPPVFDVLAVLGRDEALARLRDRLPA
>Mature_473_residues
SSPVVTRFAPSPTGFLHIGGARTALFNWLYARRFGGRMLLRIEDTDRERSTQGAIDAILDGMRWLGLDWDGDVIYQFARA
ERHREVAESLLASGNAYHCYASAEELTQMRETARAEGRAPRYDGRWRDRDPSEAPAGVKPVIRLRAPSEGETVVEDAVQG
RVTWANRDLDDLVLLRSDGTPTYMLAVVVDDHDMGVTQVIRGDDHLTNAARQSQIYRALGWDVPAMAHIPLIHGADGAKL
SKRHGALGVEEYRDRGYLPAALRNYLVRLGWSHGDQEVFSTEEMIAAFDLKAIGRSAARFDFTKLESLNGLYIRGSADSV
LVDAIDAILPARGPERGLPTALTPDLREKLVSAMPGLKERAKTLVELLDSAYYLYAARPLALDAKAEALLAGDAPERLRA
LLPALEALPEWNAATTEAAVRQFADSQGVKLGQVAQPLRAALTGRATSPPVFDVLAVLGRDEALARLRDRLPA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=497, Percent_Identity=32.1931589537223, Blast_Score=230, Evalue=2e-60,
Organism=Escherichia coli, GI1788743, Length=469, Percent_Identity=44.7761194029851, Blast_Score=390, Evalue=1e-109,
Organism=Escherichia coli, GI226510925, Length=285, Percent_Identity=36.8421052631579, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1786895, Length=132, Percent_Identity=31.0606060606061, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=486, Percent_Identity=32.3045267489712, Blast_Score=213, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6324540, Length=492, Percent_Identity=31.9105691056911, Blast_Score=245, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6321192, Length=338, Percent_Identity=25.4437869822485, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324742, Length=108, Percent_Identity=34.2592592592593, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24665321, Length=495, Percent_Identity=33.1313131313131, Blast_Score=259, Evalue=3e-69,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 52022; Mature: 51890

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPVVTRFAPSPTGFLHIGGARTALFNWLYARRFGGRMLLRIEDTDRERSTQGAIDAIL
CCCCCEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHH
DGMRWLGLDWDGDVIYQFARAERHREVAESLLASGNAYHCYASAEELTQMRETARAEGRA
CCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCC
PRYDGRWRDRDPSEAPAGVKPVIRLRAPSEGETVVEDAVQGRVTWANRDLDDLVLLRSDG
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCEEECCCCCCCEEEEECCC
TPTYMLAVVVDDHDMGVTQVIRGDDHLTNAARQSQIYRALGWDVPAMAHIPLIHGADGAK
CCEEEEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCH
LSKRHGALGVEEYRDRGYLPAALRNYLVRLGWSHGDQEVFSTEEMIAAFDLKAIGRSAAR
HHHHCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHH
FDFTKLESLNGLYIRGSADSVLVDAIDAILPARGPERGLPTALTPDLREKLVSAMPGLKE
CCHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCHHH
RAKTLVELLDSAYYLYAARPLALDAKAEALLAGDAPERLRALLPALEALPEWNAATTEAA
HHHHHHHHHHHHHEEEECCCEEECCCCHHEECCCCHHHHHHHHHHHHHCCCCCCHHHHHH
VRQFADSQGVKLGQVAQPLRAALTGRATSPPVFDVLAVLGRDEALARLRDRLPA
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SSPVVTRFAPSPTGFLHIGGARTALFNWLYARRFGGRMLLRIEDTDRERSTQGAIDAIL
CCCCEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHH
DGMRWLGLDWDGDVIYQFARAERHREVAESLLASGNAYHCYASAEELTQMRETARAEGRA
CCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCC
PRYDGRWRDRDPSEAPAGVKPVIRLRAPSEGETVVEDAVQGRVTWANRDLDDLVLLRSDG
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCCEEECCCCCCCEEEEECCC
TPTYMLAVVVDDHDMGVTQVIRGDDHLTNAARQSQIYRALGWDVPAMAHIPLIHGADGAK
CCEEEEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCH
LSKRHGALGVEEYRDRGYLPAALRNYLVRLGWSHGDQEVFSTEEMIAAFDLKAIGRSAAR
HHHHCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHH
FDFTKLESLNGLYIRGSADSVLVDAIDAILPARGPERGLPTALTPDLREKLVSAMPGLKE
CCHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCHHH
RAKTLVELLDSAYYLYAARPLALDAKAEALLAGDAPERLRALLPALEALPEWNAATTEAA
HHHHHHHHHHHHHEEEECCCEEECCCCHHEECCCCHHHHHHHHHHHHHCCCCCCHHHHHH
VRQFADSQGVKLGQVAQPLRAALTGRATSPPVFDVLAVLGRDEALARLRDRLPA
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA