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The map label for this gene is xthA [H]

Identifier: 218532288

GI number: 218532288

Start: 4651446

End: 4652255

Strand: Reverse

Name: xthA [H]

Synonym: Mchl_4400

Alternate gene names: 218532288

Gene position: 4652255-4651446 (Counterclockwise)

Preceding gene: 218532289

Following gene: 218532287

Centisome position: 80.52

GC content: 68.77

Gene sequence:

>810_bases
GTGCGCCTCACCGTCACGACCTGGAACATCAACTCGGTGCGTCTGCGCATCGAGTCCGTGCTGCGCTTCCTCCGCGAGGC
GCAGCCGGACGTGCTGTGCCTGCAGGAGACCAAGTGCCCCGACGACCTGTTCCCGCTCAAGGCGTTCAAGGGATCGGGCT
ACGAAAACATCGTCTTCGCCGGGCAGAAGGGCTATAACGGCGTCGCGATCATCTCGCGCTTTCCGCTGCTGACCCGCGAC
GTGATGAGCTTCTGCGAGCGGCCCGACGCGCGCCACATCTCGGCGGTGCTCGGGCCCGAGGCGGGACCGGCGGCGGGGAT
CGTGCTGCACGATTTCTACGTGCCGGCGGGCGGCGACATGCCCCACCGCGACCTCAACCCGAAATTCGCGCACAAGCTCG
ACTTCCTGTCCGAATTGCGGGGCTGGGGCGGACGGCGGGTCTCCGGGCCGGCGATCCTGGTGGGGGACCTCAACGTCGCG
CCGCTGGAGCACGACGTCTGGTCGCACAAGCAGCTCCTCGACGTGGTGAGCCACACCCCCGTCGAGACCGAAGCGCTCGA
GACGCTGCGGGGCGAGGCCGGCTGGATCGACGCGGCGCGGCTCCTCACCCCGGAGCCGGAAAAGATCTACACGTGGTGGA
GCTACCGCTCGCCGAACTGGGAACTCGCCAACAAGGGCCGGCGCCTCGACCATACCTGGGTTTCGCCCGATCTCGCCGGC
ACCGTGCGCCGGGTCGCGGTGTTCCGCGAGGCGCGCGGCTGGGAGAGGCCGTCCGATCACGTCCCGGTGACGTTGACGTT
GGAGCTCTGA

Upstream 100 bases:

>100_bases
TCCTGTTTCAACGCGCATTCTTTCGACGAACCGGTTTCCACTTCGTCGGAATGCGCTTTATCGAAGCGGCTCTTCACGCC
ACGCTTCTCAGGCAATCGCC

Downstream 100 bases:

>100_bases
GCCGGGAACGCGGAAGCCCGTCCAGGCATTGGGTCTCCAATCTGAGCTTTCGCAATGGAGTCCCACCGCATGCGCTCGAT
TCTGCGCCAGATCGTCGTCG

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MRLTVTTWNINSVRLRIESVLRFLREAQPDVLCLQETKCPDDLFPLKAFKGSGYENIVFAGQKGYNGVAIISRFPLLTRD
VMSFCERPDARHISAVLGPEAGPAAGIVLHDFYVPAGGDMPHRDLNPKFAHKLDFLSELRGWGGRRVSGPAILVGDLNVA
PLEHDVWSHKQLLDVVSHTPVETEALETLRGEAGWIDAARLLTPEPEKIYTWWSYRSPNWELANKGRRLDHTWVSPDLAG
TVRRVAVFREARGWERPSDHVPVTLTLEL

Sequences:

>Translated_269_residues
MRLTVTTWNINSVRLRIESVLRFLREAQPDVLCLQETKCPDDLFPLKAFKGSGYENIVFAGQKGYNGVAIISRFPLLTRD
VMSFCERPDARHISAVLGPEAGPAAGIVLHDFYVPAGGDMPHRDLNPKFAHKLDFLSELRGWGGRRVSGPAILVGDLNVA
PLEHDVWSHKQLLDVVSHTPVETEALETLRGEAGWIDAARLLTPEPEKIYTWWSYRSPNWELANKGRRLDHTWVSPDLAG
TVRRVAVFREARGWERPSDHVPVTLTLEL
>Mature_269_residues
MRLTVTTWNINSVRLRIESVLRFLREAQPDVLCLQETKCPDDLFPLKAFKGSGYENIVFAGQKGYNGVAIISRFPLLTRD
VMSFCERPDARHISAVLGPEAGPAAGIVLHDFYVPAGGDMPHRDLNPKFAHKLDFLSELRGWGGRRVSGPAILVGDLNVA
PLEHDVWSHKQLLDVVSHTPVETEALETLRGEAGWIDAARLLTPEPEKIYTWWSYRSPNWELANKGRRLDHTWVSPDLAG
TVRRVAVFREARGWERPSDHVPVTLTLEL

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=275, Percent_Identity=30.1818181818182, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI18375503, Length=275, Percent_Identity=30.1818181818182, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI18375501, Length=275, Percent_Identity=30.1818181818182, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1788046, Length=274, Percent_Identity=29.5620437956204, Blast_Score=109, Evalue=2e-25,
Organism=Drosophila melanogaster, GI221330655, Length=276, Percent_Identity=28.9855072463768, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI17136678, Length=276, Percent_Identity=28.9855072463768, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30289; Mature: 30289

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLTVTTWNINSVRLRIESVLRFLREAQPDVLCLQETKCPDDLFPLKAFKGSGYENIVFA
CEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHCCCCCCEEEEE
GQKGYNGVAIISRFPLLTRDVMSFCERPDARHISAVLGPEAGPAAGIVLHDFYVPAGGDM
CCCCCCCEEEEHHCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEEEEEECCCCCC
PHRDLNPKFAHKLDFLSELRGWGGRRVSGPAILVGDLNVAPLEHDVWSHKQLLDVVSHTP
CCCCCCHHHHHHHHHHHHHHCCCCCEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCC
VETEALETLRGEAGWIDAARLLTPEPEKIYTWWSYRSPNWELANKGRRLDHTWVSPDLAG
CCHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCHHHHCCCCCCCCCCCCCCHHH
TVRRVAVFREARGWERPSDHVPVTLTLEL
HHHHHHHHHHHCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MRLTVTTWNINSVRLRIESVLRFLREAQPDVLCLQETKCPDDLFPLKAFKGSGYENIVFA
CEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHCCCCCCEEEEE
GQKGYNGVAIISRFPLLTRDVMSFCERPDARHISAVLGPEAGPAAGIVLHDFYVPAGGDM
CCCCCCCEEEEHHCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEEEEEECCCCCC
PHRDLNPKFAHKLDFLSELRGWGGRRVSGPAILVGDLNVAPLEHDVWSHKQLLDVVSHTP
CCCCCCHHHHHHHHHHHHHHCCCCCEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCC
VETEALETLRGEAGWIDAARLLTPEPEKIYTWWSYRSPNWELANKGRRLDHTWVSPDLAG
CCHHHHHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCHHHHCCCCCCCCCCCCCCHHH
TVRRVAVFREARGWERPSDHVPVTLTLEL
HHHHHHHHHHHCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]