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The map label for this gene is potF [H]

Identifier: 218532276

GI number: 218532276

Start: 4639495

End: 4640598

Strand: Reverse

Name: potF [H]

Synonym: Mchl_4388

Alternate gene names: 218532276

Gene position: 4640598-4639495 (Counterclockwise)

Preceding gene: 218532277

Following gene: 218532271

Centisome position: 80.32

GC content: 64.58

Gene sequence:

>1104_bases
GTGATCCGGCGGCTCCTGCTGGGAGGGCTCGCCGCCCTCCCCTTCCTCCTCTGCGCGACCGGCGGGGTGCGGGCCGAGGA
GCGGGTCGTCAACATCTACAACTGGTCGGACTACATCGACCCGAAGGTGCTCGACGACTTCACCAAGGAGACGGGGATCA
AGGTCGTCTACGACACCTACGACAACAACGAGATCCTCGAGACGAAGCTCCTGGCCGGCAAGTCCGGCTACGACATCGTC
GTGCCCTCCGGCCCCTTCCTTCAGCGCCTGATCAAGGCGGGCGTGTTCCTGCCGCTCGACAAGGCGAAGATCCCAAACCT
CAAACATGCCTGGCCCGAGATCAGCACCCGGCTCCAGGCCTATGATCCGGGCAACACGTTCGCCGTCGATTACATGTGGG
GCACCACCGGCATCGGCGTGAACGTGTCGGCCGTGCGCGAGCGGCTGGGCGCCAACGCCCCCCTCAATAGCTGGAGCCTC
GTGCTCAATCCCGGCTCGATCGCGAAGCTGAAGGATTGCGGAGTCATGCTCCTCGACTCCCCCGAGGATCTGATTCCCTC
GATCCTGCCCTTCTACGGCTTCAAGTCGGATTCGAAGCGTTGGGACGACATCACCGCGGTGACCGATGCGCTCTACAAGG
TCCGCGGCGCGGTGCGGAAGTTCCACTCCTCGGAATACGTCAACGGGCTCGCCAACGGCGATGTCTGCCTCGCGGTCGGC
TATTCCGGCGACGTGATGCAGGCGAAGAAGCGCGCCGAGGAATCGAAGAACGGCATCGAGATCGCCTACTTCATCCCGAA
GGAAGGCGCGCTGATGTGGTTCGACGCCTTCGCGGTCCCGAAGGATGCCGCCCACCCGGCCGAGGCGCACGCCTTCATCG
ACTACATGATGCGTCCCGAAGTGGCGGCGGCCAACACCAACTTCGTTTCCTTCGCCAACGGCAACCTCGCCGCGCGCAAG
CTCGTGAAGCCGGAGCTTCTCAACAATCCCGGCATCTACCCCGACGAGACGACGATGCAGCGGCTCTCGGTCAACACCGC
CTGGAACGACTCGACCCAGCGCTTCGTCACCCGAGCCTGGACGCGGGTGCGCACCGGCCGCTAG

Upstream 100 bases:

>100_bases
TCGACAAGGGGAACCCGCCCGCCCGCGCCACCGTCGAGGCGCGGCTCGCCGGGCCGGAATACCTCGTCGAGATCGTCGTC
ACGGCCGTCCGGGGTAAATC

Downstream 100 bases:

>100_bases
AGCATCATCCGGAAGGGTGGTTGCTGGCTTTCGGAAAGAGATGATGCAAGAACAAAAGCTTAGAGCATCGTCCTGGATCT
GATATCCAGGACGATGCTCT

Product: extracellular solute-binding protein family 1

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MIRRLLLGGLAALPFLLCATGGVRAEERVVNIYNWSDYIDPKVLDDFTKETGIKVVYDTYDNNEILETKLLAGKSGYDIV
VPSGPFLQRLIKAGVFLPLDKAKIPNLKHAWPEISTRLQAYDPGNTFAVDYMWGTTGIGVNVSAVRERLGANAPLNSWSL
VLNPGSIAKLKDCGVMLLDSPEDLIPSILPFYGFKSDSKRWDDITAVTDALYKVRGAVRKFHSSEYVNGLANGDVCLAVG
YSGDVMQAKKRAEESKNGIEIAYFIPKEGALMWFDAFAVPKDAAHPAEAHAFIDYMMRPEVAAANTNFVSFANGNLAARK
LVKPELLNNPGIYPDETTMQRLSVNTAWNDSTQRFVTRAWTRVRTGR

Sequences:

>Translated_367_residues
MIRRLLLGGLAALPFLLCATGGVRAEERVVNIYNWSDYIDPKVLDDFTKETGIKVVYDTYDNNEILETKLLAGKSGYDIV
VPSGPFLQRLIKAGVFLPLDKAKIPNLKHAWPEISTRLQAYDPGNTFAVDYMWGTTGIGVNVSAVRERLGANAPLNSWSL
VLNPGSIAKLKDCGVMLLDSPEDLIPSILPFYGFKSDSKRWDDITAVTDALYKVRGAVRKFHSSEYVNGLANGDVCLAVG
YSGDVMQAKKRAEESKNGIEIAYFIPKEGALMWFDAFAVPKDAAHPAEAHAFIDYMMRPEVAAANTNFVSFANGNLAARK
LVKPELLNNPGIYPDETTMQRLSVNTAWNDSTQRFVTRAWTRVRTGR
>Mature_367_residues
MIRRLLLGGLAALPFLLCATGGVRAEERVVNIYNWSDYIDPKVLDDFTKETGIKVVYDTYDNNEILETKLLAGKSGYDIV
VPSGPFLQRLIKAGVFLPLDKAKIPNLKHAWPEISTRLQAYDPGNTFAVDYMWGTTGIGVNVSAVRERLGANAPLNSWSL
VLNPGSIAKLKDCGVMLLDSPEDLIPSILPFYGFKSDSKRWDDITAVTDALYKVRGAVRKFHSSEYVNGLANGDVCLAVG
YSGDVMQAKKRAEESKNGIEIAYFIPKEGALMWFDAFAVPKDAAHPAEAHAFIDYMMRPEVAAANTNFVSFANGNLAARK
LVKPELLNNPGIYPDETTMQRLSVNTAWNDSTQRFVTRAWTRVRTGR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=367, Percent_Identity=47.9564032697548, Blast_Score=370, Evalue=1e-104,
Organism=Escherichia coli, GI1787367, Length=340, Percent_Identity=31.1764705882353, Blast_Score=176, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40590; Mature: 40590

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRRLLLGGLAALPFLLCATGGVRAEERVVNIYNWSDYIDPKVLDDFTKETGIKVVYDTY
CHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEC
DNNEILETKLLAGKSGYDIVVPSGPFLQRLIKAGVFLPLDKAKIPNLKHAWPEISTRLQA
CCCCEEEHHEECCCCCCEEEECCCHHHHHHHHCCCEEECCCCCCCCCHHCCHHHHCEEEE
YDPGNTFAVDYMWGTTGIGVNVSAVRERLGANAPLNSWSLVLNPGSIAKLKDCGVMLLDS
ECCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCEEHHCCCEEEECC
PEDLIPSILPFYGFKSDSKRWDDITAVTDALYKVRGAVRKFHSSEYVNGLANGDVCLAVG
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
YSGDVMQAKKRAEESKNGIEIAYFIPKEGALMWFDAFAVPKDAAHPAEAHAFIDYMMRPE
CCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCC
VAAANTNFVSFANGNLAARKLVKPELLNNPGIYPDETTMQRLSVNTAWNDSTQRFVTRAW
CEECCCCEEEECCCCHHHHHHCCHHHCCCCCCCCCCHHHHHEEECCCCCCHHHHHHHHHH
TRVRTGR
HHHCCCC
>Mature Secondary Structure
MIRRLLLGGLAALPFLLCATGGVRAEERVVNIYNWSDYIDPKVLDDFTKETGIKVVYDTY
CHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEC
DNNEILETKLLAGKSGYDIVVPSGPFLQRLIKAGVFLPLDKAKIPNLKHAWPEISTRLQA
CCCCEEEHHEECCCCCCEEEECCCHHHHHHHHCCCEEECCCCCCCCCHHCCHHHHCEEEE
YDPGNTFAVDYMWGTTGIGVNVSAVRERLGANAPLNSWSLVLNPGSIAKLKDCGVMLLDS
ECCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCCEEHHCCCEEEECC
PEDLIPSILPFYGFKSDSKRWDDITAVTDALYKVRGAVRKFHSSEYVNGLANGDVCLAVG
CHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
YSGDVMQAKKRAEESKNGIEIAYFIPKEGALMWFDAFAVPKDAAHPAEAHAFIDYMMRPE
CCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCC
VAAANTNFVSFANGNLAARKLVKPELLNNPGIYPDETTMQRLSVNTAWNDSTQRFVTRAW
CEECCCCEEEECCCCHHHHHHCCHHHCCCCCCCCCCHHHHHEEECCCCCCHHHHHHHHHH
TRVRTGR
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]