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The map label for this gene is bioB [H]

Identifier: 218532263

GI number: 218532263

Start: 4616702

End: 4617709

Strand: Reverse

Name: bioB [H]

Synonym: Mchl_4375

Alternate gene names: 218532263

Gene position: 4617709-4616702 (Counterclockwise)

Preceding gene: 218532265

Following gene: 218532262

Centisome position: 79.92

GC content: 70.14

Gene sequence:

>1008_bases
ATGAGCGACACCGTAGTCTCCCTCACCGCCTCCCCCGCCGCCATCCGGCATGATTGGACCCTGGCTGAGATCCAGGCGAT
CCACGACATGCCGCTGCTCGATCTCGTGCATCGGGCGGGCGTCGTGCACCGGGCGCATAACGACCCCGCCGACATCCAGC
GGGCGGCCCTGCTGTCGATCAAGACCGGCGGCTGCCCCGAGGATTGCGCCTATTGCCCGCAATCGGCCCACCACAAGGGC
GCCAACCTGCCCCGCGAGCGGCTGATGCCGGTGGACGCCGTGCTGAAGGAGGCCGCCGCCGCCAAGGCCAACGGGGCGCA
CCGCTTCTGCATGGGCGCGGCGTGGCGCAAACCCAAGGATGGGCCGGATTTCGATGCGGTGCTGGAGATGGTGCGCGGCG
TGCGCGGGCTGGGCATGGAAGCCTGCGTGACGCTCGGCATGCTGACCCAGAGCCAGGCGCAGCGCCTCGCGGAAGCGGGG
CTCACCTCCTACAACCACAATCTCGACACCGGCCCTGAATTCTACGGCGACATCATCTCGACCCGGACCTACGACGACCG
GCTCCAGACGCTGGAGCATGTCCGGCAGGCCGGCATCGGCGTCTGCTGCGGCGGCATCGTCGGGATGGGCGAGCGGGTGC
GCGACAGGGCCGAGATGCTGCTGGTGCTCGCCAATCACGCCCCCCACCCGGAGAGCGTGCCGATCAACGCGCTCGTCGCC
GTCGAAGGCACGCCGCTGGAAGACCGCCCGCCGATCGATCCGCTCGATCTCGTGCGGATGTGCGCCACCGCCCGCATCGT
CATGCCCAAGGCCCGCGTGCGCCTCAGCGCCGGCCGCAAGAGCCTGACCCGCGAGGCGCAGATCCTGTGCTTCCTGGCTG
GCGCCAACTCGATCTTCTACGGCGAGCGCCTGCTCACCACCGCCAACAACGAGGCGGATGCCGACGCCGAGCTGCTGCGC
GACATCGGCGTGCCGGTGCCCGAGGTGACGCTGGCCGCCGCGGAGTAG

Upstream 100 bases:

>100_bases
CTGAATCAGGGCAGAGCGCCACGCGTGCCGGGCGCTTCGCGACCGGGCTCTCCCGCGGCAAGGCTTGATTTTTCCCGCGC
CTTCGCCCTCAAAGAAGCGC

Downstream 100 bases:

>100_bases
GGGCACGGCAAGCCGGCCCTCACCTTGACTTGAAACGCCTCCCGTGAATGGTGCCGGCTCATTCTCAAGAGCCCGCCATG
CACCGTTACCGTACCCATAC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MSDTVVSLTASPAAIRHDWTLAEIQAIHDMPLLDLVHRAGVVHRAHNDPADIQRAALLSIKTGGCPEDCAYCPQSAHHKG
ANLPRERLMPVDAVLKEAAAAKANGAHRFCMGAAWRKPKDGPDFDAVLEMVRGVRGLGMEACVTLGMLTQSQAQRLAEAG
LTSYNHNLDTGPEFYGDIISTRTYDDRLQTLEHVRQAGIGVCCGGIVGMGERVRDRAEMLLVLANHAPHPESVPINALVA
VEGTPLEDRPPIDPLDLVRMCATARIVMPKARVRLSAGRKSLTREAQILCFLAGANSIFYGERLLTTANNEADADAELLR
DIGVPVPEVTLAAAE

Sequences:

>Translated_335_residues
MSDTVVSLTASPAAIRHDWTLAEIQAIHDMPLLDLVHRAGVVHRAHNDPADIQRAALLSIKTGGCPEDCAYCPQSAHHKG
ANLPRERLMPVDAVLKEAAAAKANGAHRFCMGAAWRKPKDGPDFDAVLEMVRGVRGLGMEACVTLGMLTQSQAQRLAEAG
LTSYNHNLDTGPEFYGDIISTRTYDDRLQTLEHVRQAGIGVCCGGIVGMGERVRDRAEMLLVLANHAPHPESVPINALVA
VEGTPLEDRPPIDPLDLVRMCATARIVMPKARVRLSAGRKSLTREAQILCFLAGANSIFYGERLLTTANNEADADAELLR
DIGVPVPEVTLAAAE
>Mature_334_residues
SDTVVSLTASPAAIRHDWTLAEIQAIHDMPLLDLVHRAGVVHRAHNDPADIQRAALLSIKTGGCPEDCAYCPQSAHHKGA
NLPRERLMPVDAVLKEAAAAKANGAHRFCMGAAWRKPKDGPDFDAVLEMVRGVRGLGMEACVTLGMLTQSQAQRLAEAGL
TSYNHNLDTGPEFYGDIISTRTYDDRLQTLEHVRQAGIGVCCGGIVGMGERVRDRAEMLLVLANHAPHPESVPINALVAV
EGTPLEDRPPIDPLDLVRMCATARIVMPKARVRLSAGRKSLTREAQILCFLAGANSIFYGERLLTTANNEADADAELLRD
IGVPVPEVTLAAAE

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=311, Percent_Identity=58.1993569131833, Blast_Score=374, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321725, Length=315, Percent_Identity=47.9365079365079, Blast_Score=317, Evalue=2e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 36064; Mature: 35933

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTVVSLTASPAAIRHDWTLAEIQAIHDMPLLDLVHRAGVVHRAHNDPADIQRAALLSI
CCCCEEEEECCCCHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHEEE
KTGGCPEDCAYCPQSAHHKGANLPRERLMPVDAVLKEAAAAKANGAHRFCMGAAWRKPKD
CCCCCCHHHHHCCCHHCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
GPDFDAVLEMVRGVRGLGMEACVTLGMLTQSQAQRLAEAGLTSYNHNLDTGPEFYGDIIS
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
TRTYDDRLQTLEHVRQAGIGVCCGGIVGMGERVRDRAEMLLVLANHAPHPESVPINALVA
CCCHHHHHHHHHHHHHCCCCEEHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
VEGTPLEDRPPIDPLDLVRMCATARIVMPKARVRLSAGRKSLTREAQILCFLAGANSIFY
ECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCEEE
GERLLTTANNEADADAELLRDIGVPVPEVTLAAAE
CCCCEEECCCCCCHHHHHHHHCCCCCCCEEEEECC
>Mature Secondary Structure 
SDTVVSLTASPAAIRHDWTLAEIQAIHDMPLLDLVHRAGVVHRAHNDPADIQRAALLSI
CCCEEEEECCCCHHHCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHEEE
KTGGCPEDCAYCPQSAHHKGANLPRERLMPVDAVLKEAAAAKANGAHRFCMGAAWRKPKD
CCCCCCHHHHHCCCHHCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCC
GPDFDAVLEMVRGVRGLGMEACVTLGMLTQSQAQRLAEAGLTSYNHNLDTGPEFYGDIIS
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
TRTYDDRLQTLEHVRQAGIGVCCGGIVGMGERVRDRAEMLLVLANHAPHPESVPINALVA
CCCHHHHHHHHHHHHHCCCCEEHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
VEGTPLEDRPPIDPLDLVRMCATARIVMPKARVRLSAGRKSLTREAQILCFLAGANSIFY
ECCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCEEE
GERLLTTANNEADADAELLRDIGVPVPEVTLAAAE
CCCCEEECCCCCCHHHHHHHHCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA