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The map label for this gene is greA [H]

Identifier: 218532092

GI number: 218532092

Start: 4412149

End: 4412613

Strand: Reverse

Name: greA [H]

Synonym: Mchl_4186

Alternate gene names: 218532092

Gene position: 4412613-4412149 (Counterclockwise)

Preceding gene: 218532093

Following gene: 218532086

Centisome position: 76.37

GC content: 67.53

Gene sequence:

>465_bases
ATGAGCAGAGCATTCGTCCGTGAGCCGGAAGGTGGCGAGGCCTTCGAGGATCTTCCCGATCGGCCCATCTCCTCCCACAC
CAACTTCGTGACGCCTGAGGGCCTTGCGCAGATCGAGGCCGAGGTGGCGCGCTTGGAGGCGGAGCTGTCCGGCCTCTCGC
CGCAGCAGGACAAGGCCGACCACATCCGTGTCTCGCGCGATCTGCGCTATTGGACCGCGCGGAAGAACTCGGCCGAACCG
GTCGAAGCGTTCGAGGGCGATACCGTGCGTTTCGGCGCGACGGTGACCGTGCTGCGCGAGGACGACACGCGCCAGACCTT
CCGCATCGTCGGCGAGGACGAGGCCGACCCGTCCGCCGGGTCGATCTCCTACGTCGCGCCGCTGGCCCAAGCGCTCACCG
GCAAGGGCGTCGGCGACACCGTGACGGTCAACGACCACGAGTTCGAGATCGCCGAGATCCGTTGA

Upstream 100 bases:

>100_bases
CGGGCGGCCTCGCCGAAACCCTGCTCGACGGTCAGGTGCGGGGCCGGACCGTCGTCGCCATCGGTGGAACCGCGGCTTAG
GCTTGGGCATTTCGTATTTT

Downstream 100 bases:

>100_bases
GGTCGAAGGTTTCGAAAGGACGAGTCCTTTCGCGGGTCCAGGGCAGAGCCCTGGAGTAAGATCTGGGCGCTGCCCCGAGA
CCCCGCCAAAGGGATGATCC

Product: GreA/GreB family elongation factor

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MSRAFVREPEGGEAFEDLPDRPISSHTNFVTPEGLAQIEAEVARLEAELSGLSPQQDKADHIRVSRDLRYWTARKNSAEP
VEAFEGDTVRFGATVTVLREDDTRQTFRIVGEDEADPSAGSISYVAPLAQALTGKGVGDTVTVNDHEFEIAEIR

Sequences:

>Translated_154_residues
MSRAFVREPEGGEAFEDLPDRPISSHTNFVTPEGLAQIEAEVARLEAELSGLSPQQDKADHIRVSRDLRYWTARKNSAEP
VEAFEGDTVRFGATVTVLREDDTRQTFRIVGEDEADPSAGSISYVAPLAQALTGKGVGDTVTVNDHEFEIAEIR
>Mature_153_residues
SRAFVREPEGGEAFEDLPDRPISSHTNFVTPEGLAQIEAEVARLEAELSGLSPQQDKADHIRVSRDLRYWTARKNSAEPV
EAFEGDTVRFGATVTVLREDDTRQTFRIVGEDEADPSAGSISYVAPLAQALTGKGVGDTVTVNDHEFEIAEIR

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

None

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 16902; Mature: 16770

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRAFVREPEGGEAFEDLPDRPISSHTNFVTPEGLAQIEAEVARLEAELSGLSPQQDKAD
CCCCCCCCCCCCCHHHHCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
HIRVSRDLRYWTARKNSAEPVEAFEGDTVRFGATVTVLREDDTRQTFRIVGEDEADPSAG
CEEECCCCEEEECCCCCCCCHHHCCCCEEEECEEEEEEECCCCCCEEEEECCCCCCCCCC
SISYVAPLAQALTGKGVGDTVTVNDHEFEIAEIR
CCHHHHHHHHHHHCCCCCCEEEECCCCEEEEECC
>Mature Secondary Structure 
SRAFVREPEGGEAFEDLPDRPISSHTNFVTPEGLAQIEAEVARLEAELSGLSPQQDKAD
CCCCCCCCCCCCHHHHCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
HIRVSRDLRYWTARKNSAEPVEAFEGDTVRFGATVTVLREDDTRQTFRIVGEDEADPSAG
CEEECCCCEEEECCCCCCCCHHHCCCCEEEECEEEEEEECCCCCCEEEEECCCCCCCCCC
SISYVAPLAQALTGKGVGDTVTVNDHEFEIAEIR
CCHHHHHHHHHHHCCCCCCEEEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA