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The map label for this gene is purD [H]

Identifier: 218532086

GI number: 218532086

Start: 4406317

End: 4407624

Strand: Reverse

Name: purD [H]

Synonym: Mchl_4180

Alternate gene names: 218532086

Gene position: 4407624-4406317 (Counterclockwise)

Preceding gene: 218532092

Following gene: 218532085

Centisome position: 76.28

GC content: 72.63

Gene sequence:

>1308_bases
ATGACCGAGCCCTTCTCCATCCTGCTCATCGGCTCCGGCGGGCGCGAGCACGCGCTGGCCTGGGCCATCGCGAAATCGCC
GCTCTGCGCGCGGCTGTTCATCGCCCCCGGCAATCCGGGGACGGCGCAGCACGGCGAGAACCGGCCGGACCTCGCGGTGT
CCGATCACGCCGCCGTCGTCGCGTTCTGCCGGGCGGAAGGGGTCGGGCTGGTGGTGGTGGGGCCGGAGGCGCCGCTGGTG
GCGGGCCTCGTCGACGACCTTCAGGCCGCCGGCATCCCCGCCTTCGGGCCGACGAAGGCCGCGGCGCAGCTCGAAGGCTC
GAAGGGCTTCACCAAGGATCTGTGCGCCGAGCACGACATCCCGACCGCGGCCTTCGCCCGCTTCACCGACCTCGAGCCGG
CTTTGGCCTATCTCCGCGAGCGCGGTGCGCCGATCGTGGTCAAGGCCGACGGGCTCGCCGCCGGCAAGGGCGTGACCGTG
GCCGAGACGCTGCCCGAGGCCGAGGCCGCCGTGCGGGCGATCCTCGACGGAACGGAAGGCGGGGCGCTCGTCATCGAGGA
ATGCCTGTTCGGCGAGGAGGCGAGCTTCTTCGCGCTCTGCGACGGCACCCGTGCGGTTCCGATCGGCACGGCGCAGGACC
ACAAGCGCGTCCATGACGGCGACCTCGGCCCGAATACCGGGGGCATGGGCGCCTATTCCCCCGCCTCCATCGTCACGCCG
GAGATCACCGAGACGGTGATGACGCGGATCATCGCGCCGACGCTGGCCGGCATGGCCGCCCGCAGCACGCCGTTTTCCGG
CATCCTCTATGCCGGGCTGATGCTGACGGCAGAGGGCCCCAAGCTCATCGAGTACAACACCCGCTTCGGCGATCCGGAGG
CGCAGGTGTTGATGCCGCGCCTGACCGGCGATCTCGTGCCGGCGCTGCTGGCGGCGGCTCAAGGAGACCTCTCCGGCGTC
GCCATCGGTTTCGACGCCTCCCGCGCCGCGCTCACCGTGGTGATGGCGGCGCAGGGCTATCCCGGCGCGGTGACCCGCGG
CACGGAGATCCGCGGCGTCGAGGCGGCCGAGGACGAGGCGGGCGACGCGACGGTTCTGGTGTTCCAGGCCGGCACCCGCC
GCGACGGCGACCGGCTGCTCGCCGATGGCGGCCGGGTGCTCGCGGTCACGGCGCTCGGCGCCAGCGTCGGGCAGGCGAAA
GACCGCGCCTACGCGGCGGTGAAGCGCATCGACTGGCCGGACGGGTTCTTCCGCAGCGATATCGGCGGCCGGGAGGTCGC
CCGCGAAGCAGCTTCCAAGGGCGACTGA

Upstream 100 bases:

>100_bases
CGATCTGTCGGCGCCATGCGGCGGCTTCTCCCGTGCGTTGCCGGGGCAAACGCGAAACGCCCTTTCCCCGGCCCAAAATT
TTTTGGATAGGGTTCTTCTC

Downstream 100 bases:

>100_bases
TGAAGGGCGACTGATGCCCGATTTCCTCGACCTCGCCTTCGCCGCCGCCCGCGAGGCCGCGGCTCTGGGCGAGGTACCGG
TCGGCGCGGCCGTGGTGCGG

Product: phosphoribosylamine/glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MTEPFSILLIGSGGREHALAWAIAKSPLCARLFIAPGNPGTAQHGENRPDLAVSDHAAVVAFCRAEGVGLVVVGPEAPLV
AGLVDDLQAAGIPAFGPTKAAAQLEGSKGFTKDLCAEHDIPTAAFARFTDLEPALAYLRERGAPIVVKADGLAAGKGVTV
AETLPEAEAAVRAILDGTEGGALVIEECLFGEEASFFALCDGTRAVPIGTAQDHKRVHDGDLGPNTGGMGAYSPASIVTP
EITETVMTRIIAPTLAGMAARSTPFSGILYAGLMLTAEGPKLIEYNTRFGDPEAQVLMPRLTGDLVPALLAAAQGDLSGV
AIGFDASRAALTVVMAAQGYPGAVTRGTEIRGVEAAEDEAGDATVLVFQAGTRRDGDRLLADGGRVLAVTALGASVGQAK
DRAYAAVKRIDWPDGFFRSDIGGREVAREAASKGD

Sequences:

>Translated_435_residues
MTEPFSILLIGSGGREHALAWAIAKSPLCARLFIAPGNPGTAQHGENRPDLAVSDHAAVVAFCRAEGVGLVVVGPEAPLV
AGLVDDLQAAGIPAFGPTKAAAQLEGSKGFTKDLCAEHDIPTAAFARFTDLEPALAYLRERGAPIVVKADGLAAGKGVTV
AETLPEAEAAVRAILDGTEGGALVIEECLFGEEASFFALCDGTRAVPIGTAQDHKRVHDGDLGPNTGGMGAYSPASIVTP
EITETVMTRIIAPTLAGMAARSTPFSGILYAGLMLTAEGPKLIEYNTRFGDPEAQVLMPRLTGDLVPALLAAAQGDLSGV
AIGFDASRAALTVVMAAQGYPGAVTRGTEIRGVEAAEDEAGDATVLVFQAGTRRDGDRLLADGGRVLAVTALGASVGQAK
DRAYAAVKRIDWPDGFFRSDIGGREVAREAASKGD
>Mature_434_residues
TEPFSILLIGSGGREHALAWAIAKSPLCARLFIAPGNPGTAQHGENRPDLAVSDHAAVVAFCRAEGVGLVVVGPEAPLVA
GLVDDLQAAGIPAFGPTKAAAQLEGSKGFTKDLCAEHDIPTAAFARFTDLEPALAYLRERGAPIVVKADGLAAGKGVTVA
ETLPEAEAAVRAILDGTEGGALVIEECLFGEEASFFALCDGTRAVPIGTAQDHKRVHDGDLGPNTGGMGAYSPASIVTPE
ITETVMTRIIAPTLAGMAARSTPFSGILYAGLMLTAEGPKLIEYNTRFGDPEAQVLMPRLTGDLVPALLAAAQGDLSGVA
IGFDASRAALTVVMAAQGYPGAVTRGTEIRGVEAAEDEAGDATVLVFQAGTRRDGDRLLADGGRVLAVTALGASVGQAKD
RAYAAVKRIDWPDGFFRSDIGGREVAREAASKGD

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=427, Percent_Identity=44.9648711943794, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI209869995, Length=427, Percent_Identity=44.9648711943794, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI209869993, Length=427, Percent_Identity=44.9648711943794, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI28416899, Length=427, Percent_Identity=44.9648711943794, Blast_Score=346, Evalue=3e-95,
Organism=Escherichia coli, GI1790438, Length=432, Percent_Identity=54.1666666666667, Blast_Score=429, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17567511, Length=430, Percent_Identity=39.0697674418605, Blast_Score=273, Evalue=9e-74,
Organism=Saccharomyces cerevisiae, GI6321203, Length=452, Percent_Identity=39.6017699115044, Blast_Score=322, Evalue=7e-89,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=41.3953488372093, Blast_Score=281, Evalue=9e-76,
Organism=Drosophila melanogaster, GI24582400, Length=438, Percent_Identity=41.0958904109589, Blast_Score=280, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44622; Mature: 44491

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPFSILLIGSGGREHALAWAIAKSPLCARLFIAPGNPGTAQHGENRPDLAVSDHAAVV
CCCCEEEEEECCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCEEECCCCEEE
AFCRAEGVGLVVVGPEAPLVAGLVDDLQAAGIPAFGPTKAAAQLEGSKGFTKDLCAEHDI
EEEECCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHEECCCCCCHHHHHHCCCC
PTAAFARFTDLEPALAYLRERGAPIVVKADGLAAGKGVTVAETLPEAEAAVRAILDGTEG
CHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEHHHHCCHHHHHHHHHHCCCCC
GALVIEECLFGEEASFFALCDGTRAVPIGTAQDHKRVHDGDLGPNTGGMGAYSPASIVTP
CCEEEHHHHCCCCCCEEEEECCCEEEECCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
EITETVMTRIIAPTLAGMAARSTPFSGILYAGLMLTAEGPKLIEYNTRFGDPEAQVLMPR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEEECCCCEEEEECCCCCCCHHEEEEHH
LTGDLVPALLAAAQGDLSGVAIGFDASRAALTVVMAAQGYPGAVTRGTEIRGVEAAEDEA
HHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCEECCCCCCCCCC
GDATVLVFQAGTRRDGDRLLADGGRVLAVTALGASVGQAKDRAYAAVKRIDWPDGFFRSD
CCEEEEEEECCCCCCCCEEEECCCCEEEEEEECCHHCHHHHHHHHHHHHCCCCCCHHHCC
IGGREVAREAASKGD
CCHHHHHHHHHCCCC
>Mature Secondary Structure 
TEPFSILLIGSGGREHALAWAIAKSPLCARLFIAPGNPGTAQHGENRPDLAVSDHAAVV
CCCEEEEEECCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCEEECCCCEEE
AFCRAEGVGLVVVGPEAPLVAGLVDDLQAAGIPAFGPTKAAAQLEGSKGFTKDLCAEHDI
EEEECCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHEECCCCCCHHHHHHCCCC
PTAAFARFTDLEPALAYLRERGAPIVVKADGLAAGKGVTVAETLPEAEAAVRAILDGTEG
CHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEHHHHCCHHHHHHHHHHCCCCC
GALVIEECLFGEEASFFALCDGTRAVPIGTAQDHKRVHDGDLGPNTGGMGAYSPASIVTP
CCEEEHHHHCCCCCCEEEEECCCEEEECCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
EITETVMTRIIAPTLAGMAARSTPFSGILYAGLMLTAEGPKLIEYNTRFGDPEAQVLMPR
HHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEEECCCCEEEEECCCCCCCHHEEEEHH
LTGDLVPALLAAAQGDLSGVAIGFDASRAALTVVMAAQGYPGAVTRGTEIRGVEAAEDEA
HHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCEECCCCCCCCCC
GDATVLVFQAGTRRDGDRLLADGGRVLAVTALGASVGQAKDRAYAAVKRIDWPDGFFRSD
CCEEEEEEECCCCCCCCEEEECCCCEEEEEEECCHHCHHHHHHHHHHHHCCCCCCHHHCC
IGGREVAREAASKGD
CCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]