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The map label for this gene is rsmD [H]

Identifier: 218532075

GI number: 218532075

Start: 4395822

End: 4396376

Strand: Reverse

Name: rsmD [H]

Synonym: Mchl_4169

Alternate gene names: 218532075

Gene position: 4396376-4395822 (Counterclockwise)

Preceding gene: 218532076

Following gene: 218532074

Centisome position: 76.09

GC content: 75.14

Gene sequence:

>555_bases
GTGCGGATCGTCGGCGGCGAATGGCGCGGGCGCAGGCTGGTCGGACCGAAGGCGGAGGGCATCCGCCCGACCTCCGACCG
CCTGCGCGAGGCCCTGTTCAACGTGCTCGCCCACGCCTACGACGACGCGGTTGAGGATGCGGTGGTGCTCGACCTGTTCG
CGGGTACCGGCGCCCTCGGCTTCGAGGCGCTGTCCCGCGGCGCGGCCCGCGCGCTCTTCGTGGACGAGGGGCGCCAGGCC
GGCACCCTGATCCGCGCCGCGATCGCCGCCCTTGGCTGCGAGGACCGCGCCCGGCTGATCCCGCGCGACGCGACCCGGCT
CGGACCTGCCCTGGACCGGCCCGCGACGCTGGTCTTCTGCGATCCGCCCTACCGGAAGAACCTTGCGCCCGCGGCTCTCA
CGGCCGCGGCCGGGGGCGGCTGGCTCAGCCCCGGCGCGCTGGTCCTCGTCGAGGAGGCCGCCGAGGCCGGCCCGGTCCTG
CCACCGGGTTTCGAGGAACTGGAGCGGCGCAGCTACGGCGAGACGGTGGTGACCTTCGGTCGCTTTGCGGGTTGA

Upstream 100 bases:

>100_bases
TGCCCCCGGGGGACGCCCCGGCGGTGGCGGCAAGCCGGCACCGCGCGGGGGCGGCCGTCCGAGCCGTCCGCCGCGTGGCG
AGGGCTAAGGGGCTTCGCGC

Downstream 100 bases:

>100_bases
GCCCTTCGCCCGCGCCGTGAGGTCGCCCTATCTCGCTCGGGCCACGCCCGTCCGCGAGAGGAGCCCGCCTCCATGCCGCC
CGTCCCGCCGCTTTCCCGCC

Product: methyltransferase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MRIVGGEWRGRRLVGPKAEGIRPTSDRLREALFNVLAHAYDDAVEDAVVLDLFAGTGALGFEALSRGAARALFVDEGRQA
GTLIRAAIAALGCEDRARLIPRDATRLGPALDRPATLVFCDPPYRKNLAPAALTAAAGGGWLSPGALVLVEEAAEAGPVL
PPGFEELERRSYGETVVTFGRFAG

Sequences:

>Translated_184_residues
MRIVGGEWRGRRLVGPKAEGIRPTSDRLREALFNVLAHAYDDAVEDAVVLDLFAGTGALGFEALSRGAARALFVDEGRQA
GTLIRAAIAALGCEDRARLIPRDATRLGPALDRPATLVFCDPPYRKNLAPAALTAAAGGGWLSPGALVLVEEAAEAGPVL
PPGFEELERRSYGETVVTFGRFAG
>Mature_184_residues
MRIVGGEWRGRRLVGPKAEGIRPTSDRLREALFNVLAHAYDDAVEDAVVLDLFAGTGALGFEALSRGAARALFVDEGRQA
GTLIRAAIAALGCEDRARLIPRDATRLGPALDRPATLVFCDPPYRKNLAPAALTAAAGGGWLSPGALVLVEEAAEAGPVL
PPGFEELERRSYGETVVTFGRFAG

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=165, Percent_Identity=40.6060606060606, Blast_Score=117, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 19423; Mature: 19423

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIVGGEWRGRRLVGPKAEGIRPTSDRLREALFNVLAHAYDDAVEDAVVLDLFAGTGALG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
FEALSRGAARALFVDEGRQAGTLIRAAIAALGCEDRARLIPRDATRLGPALDRPATLVFC
HHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHCCCCCHHHCCCCCCCCCEEEEE
DPPYRKNLAPAALTAAAGGGWLSPGALVLVEEAAEAGPVLPPGFEELERRSYGETVVTFG
CCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECHHHCCCCCCCCHHHHHHHHCCCHHHHHH
RFAG
CCCC
>Mature Secondary Structure
MRIVGGEWRGRRLVGPKAEGIRPTSDRLREALFNVLAHAYDDAVEDAVVLDLFAGTGALG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
FEALSRGAARALFVDEGRQAGTLIRAAIAALGCEDRARLIPRDATRLGPALDRPATLVFC
HHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCHHHCCCCCHHHCCCCCCCCCEEEEE
DPPYRKNLAPAALTAAAGGGWLSPGALVLVEEAAEAGPVLPPGFEELERRSYGETVVTFG
CCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECHHHCCCCCCCCHHHHHHHHCCCHHHHHH
RFAG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]