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The map label for this gene is pecT [H]

Identifier: 218532037

GI number: 218532037

Start: 4353077

End: 4353946

Strand: Reverse

Name: pecT [H]

Synonym: Mchl_4131

Alternate gene names: 218532037

Gene position: 4353946-4353077 (Counterclockwise)

Preceding gene: 218532044

Following gene: 218532035

Centisome position: 75.36

GC content: 69.77

Gene sequence:

>870_bases
ATGAGCAACCTCAATGTCGACCAGATCCGCTCCTTCCTGGCCGTCCTTGAAAGCGGCAGCTTCACGGCCGCTGCGCAGAC
CCTCGGCCTGCAGCAATCCACGGTCAGCGGGCATATCGCGCGGCTGGAACAGGCGCTGGGCCGCTCCCTCCTCATGCGGG
ACACGCATCGAGTCGCCCTGACGCCGGACGGCCAAGCCATGATCGGCTTTGCGCGCGACGTGCTGGAGGCGCACGACCGG
CTTCGCGCCTTCTTCTCGCCGGGCGGCCTGCGCGGCCGCATCCGCCTCGGCGTCTCCGAGGACTACACGCTGTCGGCCCT
GGCCCAGGTTCTGGCGCGGTTCGCGGATGGCCACCCTTCCGTCGATCTGCAGCTGACGGTCGGTCTCAGCCGGGCCCTCT
ACCAGGGCTATGATGCGGGCGAACTGGACGTGATCTTCTGCAAGCGCCGACGCGGCGATCCGCGCGGTGAATTGGCCTGG
GCGGAAGAGCTGATCTGGGTGGGCCGGCCCGGCTTCGCGCCCGATCCGGCCTTGCCCCTGCCCTTGGTCCTCTATCCGCC
GCCCTCGATCACGCGCACCCTGGCGCTCGATGCCCTCGAAGCGGCGGGGCGTTCCTGGCGGATCGCCTGCACCAGCGGCA
GCCTAATGGGGCTGCGCGCCGCCGTCGAAGCCGGTCTGGGCATCGCGCCGCACTCGGCCCAGGTGACGCCACCGGGACTG
GCTCCGATCCCTGAGACGGCCGGATTGCCGCCGATCGGTGAGGTCGAGTTCGTGGTGCTCGGCTCGGGCCGACAGCATCC
CGCCGCGACGGCGTTACAGGATGCGATCCTCGCAAGCACGGCGGAGCTTCAAGCCGTTCCCGAAGGGTGA

Upstream 100 bases:

>100_bases
CACTGAGAGGCTCGGCGATGGTGCTACGAATCGGGTTCGGCCCGGACCCTAAGCCGGCCAGGCTCCCGAGCGGTAAGGAT
ATCGGCTATCACGAACCGTG

Downstream 100 bases:

>100_bases
GTCTCTCGATGCCGAGCCTCGCGCCGCACGTCCCTAGGGCGATGGCCGGTGGCAAGGAGAAATGGAAGTAAGACCGAAAC
AAACGCCTTCGGATGCGAGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Pectinase gene transcriptional regulator [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSNLNVDQIRSFLAVLESGSFTAAAQTLGLQQSTVSGHIARLEQALGRSLLMRDTHRVALTPDGQAMIGFARDVLEAHDR
LRAFFSPGGLRGRIRLGVSEDYTLSALAQVLARFADGHPSVDLQLTVGLSRALYQGYDAGELDVIFCKRRRGDPRGELAW
AEELIWVGRPGFAPDPALPLPLVLYPPPSITRTLALDALEAAGRSWRIACTSGSLMGLRAAVEAGLGIAPHSAQVTPPGL
APIPETAGLPPIGEVEFVVLGSGRQHPAATALQDAILASTAELQAVPEG

Sequences:

>Translated_289_residues
MSNLNVDQIRSFLAVLESGSFTAAAQTLGLQQSTVSGHIARLEQALGRSLLMRDTHRVALTPDGQAMIGFARDVLEAHDR
LRAFFSPGGLRGRIRLGVSEDYTLSALAQVLARFADGHPSVDLQLTVGLSRALYQGYDAGELDVIFCKRRRGDPRGELAW
AEELIWVGRPGFAPDPALPLPLVLYPPPSITRTLALDALEAAGRSWRIACTSGSLMGLRAAVEAGLGIAPHSAQVTPPGL
APIPETAGLPPIGEVEFVVLGSGRQHPAATALQDAILASTAELQAVPEG
>Mature_288_residues
SNLNVDQIRSFLAVLESGSFTAAAQTLGLQQSTVSGHIARLEQALGRSLLMRDTHRVALTPDGQAMIGFARDVLEAHDRL
RAFFSPGGLRGRIRLGVSEDYTLSALAQVLARFADGHPSVDLQLTVGLSRALYQGYDAGELDVIFCKRRRGDPRGELAWA
EELIWVGRPGFAPDPALPLPLVLYPPPSITRTLALDALEAAGRSWRIACTSGSLMGLRAAVEAGLGIAPHSAQVTPPGLA
PIPETAGLPPIGEVEFVVLGSGRQHPAATALQDAILASTAELQAVPEG

Specific function: Regulates pectinase gene expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788626, Length=266, Percent_Identity=29.3233082706767, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1790208, Length=143, Percent_Identity=32.1678321678322, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787601, Length=177, Percent_Identity=27.1186440677966, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI145693193, Length=124, Percent_Identity=33.0645161290323, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790262, Length=245, Percent_Identity=26.530612244898, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787589, Length=146, Percent_Identity=32.1917808219178, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 30546; Mature: 30415

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLNVDQIRSFLAVLESGSFTAAAQTLGLQQSTVSGHIARLEQALGRSLLMRDTHRVAL
CCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
TPDGQAMIGFARDVLEAHDRLRAFFSPGGLRGRIRLGVSEDYTLSALAQVLARFADGHPS
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCC
VDLQLTVGLSRALYQGYDAGELDVIFCKRRRGDPRGELAWAEELIWVGRPGFAPDPALPL
EEEEEEECHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHEEEECCCCCCCCCCCCC
PLVLYPPPSITRTLALDALEAAGRSWRIACTSGSLMGLRAAVEAGLGIAPHSAQVTPPGL
CEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
APIPETAGLPPIGEVEFVVLGSGRQHPAATALQDAILASTAELQAVPEG
CCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCEECCCC
>Mature Secondary Structure 
SNLNVDQIRSFLAVLESGSFTAAAQTLGLQQSTVSGHIARLEQALGRSLLMRDTHRVAL
CCCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
TPDGQAMIGFARDVLEAHDRLRAFFSPGGLRGRIRLGVSEDYTLSALAQVLARFADGHPS
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCC
VDLQLTVGLSRALYQGYDAGELDVIFCKRRRGDPRGELAWAEELIWVGRPGFAPDPALPL
EEEEEEECHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHEEEECCCCCCCCCCCCC
PLVLYPPPSITRTLALDALEAAGRSWRIACTSGSLMGLRAAVEAGLGIAPHSAQVTPPGL
CEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
APIPETAGLPPIGEVEFVVLGSGRQHPAATALQDAILASTAELQAVPEG
CCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626286 [H]