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The map label for this gene is rfbD [C]

Identifier: 218531918

GI number: 218531918

Start: 4212650

End: 4213540

Strand: Reverse

Name: rfbD [C]

Synonym: Mchl_4002

Alternate gene names: 218531918

Gene position: 4213540-4212650 (Counterclockwise)

Preceding gene: 218531919

Following gene: 218531917

Centisome position: 72.93

GC content: 73.29

Gene sequence:

>891_bases
ATGGACATCCTGATCCTCGGCGGCGCCGGCCAGGTCGGCACGGAGCTTCAGGCCTTGTCCTGGCCCGAGGGCGTGCGGGT
GCACGCACCCGACCGCCAGAGCCTCGACATCACCGACGAGGCGGCCGTTGCGGCTGCCCTCGATGCGCGCACCTACGCGG
CGGTGATCAACACGGCCGCCTACACCGCCGTCGATAAGGCCGAAAGCGAGGTCGTCGCCGCATGGCGCCTCAACGCGCTG
GCCCCGGCCATCCTCGCGGCCGAGACGAAGCGGCGGGCCATCCCGCTCGTCCATGTCTCGACCGACTACGTGTTCGATGG
GTCGGGCGAAGGCTTCTACGCCCCCGATGCGCCGGTGAACCCGCAGAGCGTCTACGGCGCCAGCAAGGCGGCCGGCGAGA
TGGCGGTGCGCTCCGGCAATCCGCGCCACGCGATCGTGCGCACCGCCTGGGTGGTGAGCCCGCACCGGGGCAACTTCGTC
AAGACGATGCTGCGGCTCGCGGGCGAACGGGATCGGCTCACCGTTGTCGAGGACCAGCACGGCTGCCCGACCTCCGCGGC
CGATCTCGCGGGCGCGCTCGCCGCGATCGCCCTGCGGCTGGCGGGCGACGAGGCCGCGCCCACCGGTACCTTCCACTGCG
TCAACGACGGCGCGACCACGTGGTGCGACTTCGCCCGCGCGATCGTCGCGGGGTCGGCCCGCCGCGGCGGACGCTCGGTG
CCGGTCGAGGGCATCCCGAGTTCCGCCTACCCGACGCCGGCCCGGCGCCCGGTGAACTCGCGCCTCTCCACCCAGAGCCT
GACCGACGCCTACGGCCTCGCGCCGCGCTCCTGGGAGCCGGCGCTCGACGACATCCTCGACCGGCTGGTCGGGCCGGTTC
GCTCCCACTGA

Upstream 100 bases:

>100_bases
CCGTGCGCTGGTATCTCGACAACGAGGCGTGGTGGCGCCCGATCCGCGAGGGCCGCTATTCCGGCGAGCGCCTCGGCCTC
GCGCCCAAGAGCGCCTGAGC

Downstream 100 bases:

>100_bases
TCAGGACGTGTTTCGTATGAAGGGCATCGTGCTCGCCGGCGGCTCCGGCACCAGGCTCCATCCGGCCACGCTGGCGATCA
ATAAGCAGCTGCTGCCGGTC

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MDILILGGAGQVGTELQALSWPEGVRVHAPDRQSLDITDEAAVAAALDARTYAAVINTAAYTAVDKAESEVVAAWRLNAL
APAILAAETKRRAIPLVHVSTDYVFDGSGEGFYAPDAPVNPQSVYGASKAAGEMAVRSGNPRHAIVRTAWVVSPHRGNFV
KTMLRLAGERDRLTVVEDQHGCPTSAADLAGALAAIALRLAGDEAAPTGTFHCVNDGATTWCDFARAIVAGSARRGGRSV
PVEGIPSSAYPTPARRPVNSRLSTQSLTDAYGLAPRSWEPALDDILDRLVGPVRSH

Sequences:

>Translated_296_residues
MDILILGGAGQVGTELQALSWPEGVRVHAPDRQSLDITDEAAVAAALDARTYAAVINTAAYTAVDKAESEVVAAWRLNAL
APAILAAETKRRAIPLVHVSTDYVFDGSGEGFYAPDAPVNPQSVYGASKAAGEMAVRSGNPRHAIVRTAWVVSPHRGNFV
KTMLRLAGERDRLTVVEDQHGCPTSAADLAGALAAIALRLAGDEAAPTGTFHCVNDGATTWCDFARAIVAGSARRGGRSV
PVEGIPSSAYPTPARRPVNSRLSTQSLTDAYGLAPRSWEPALDDILDRLVGPVRSH
>Mature_296_residues
MDILILGGAGQVGTELQALSWPEGVRVHAPDRQSLDITDEAAVAAALDARTYAAVINTAAYTAVDKAESEVVAAWRLNAL
APAILAAETKRRAIPLVHVSTDYVFDGSGEGFYAPDAPVNPQSVYGASKAAGEMAVRSGNPRHAIVRTAWVVSPHRGNFV
KTMLRLAGERDRLTVVEDQHGCPTSAADLAGALAAIALRLAGDEAAPTGTFHCVNDGATTWCDFARAIVAGSARRGGRSV
PVEGIPSSAYPTPARRPVNSRLSTQSLTDAYGLAPRSWEPALDDILDRLVGPVRSH

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=286, Percent_Identity=25.8741258741259, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI11034825, Length=286, Percent_Identity=25.8741258741259, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1788352, Length=297, Percent_Identity=39.3939393939394, Blast_Score=187, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 31093; Mature: 31093

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDILILGGAGQVGTELQALSWPEGVRVHAPDRQSLDITDEAAVAAALDARTYAAVINTAA
CEEEEECCCCCCCCCCEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YTAVDKAESEVVAAWRLNALAPAILAAETKRRAIPLVHVSTDYVFDGSGEGFYAPDAPVN
HHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHCCEEEEEEECCEEECCCCCCEECCCCCCC
PQSVYGASKAAGEMAVRSGNPRHAIVRTAWVVSPHRGNFVKTMLRLAGERDRLTVVEDQH
HHHHCCCHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCC
GCPTSAADLAGALAAIALRLAGDEAAPTGTFHCVNDGATTWCDFARAIVAGSARRGGRSV
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCC
PVEGIPSSAYPTPARRPVNSRLSTQSLTDAYGLAPRSWEPALDDILDRLVGPVRSH
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDILILGGAGQVGTELQALSWPEGVRVHAPDRQSLDITDEAAVAAALDARTYAAVINTAA
CEEEEECCCCCCCCCCEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YTAVDKAESEVVAAWRLNALAPAILAAETKRRAIPLVHVSTDYVFDGSGEGFYAPDAPVN
HHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHCCEEEEEEECCEEECCCCCCEECCCCCCC
PQSVYGASKAAGEMAVRSGNPRHAIVRTAWVVSPHRGNFVKTMLRLAGERDRLTVVEDQH
HHHHCCCHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCC
GCPTSAADLAGALAAIALRLAGDEAAPTGTFHCVNDGATTWCDFARAIVAGSARRGGRSV
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCC
PVEGIPSSAYPTPARRPVNSRLSTQSLTDAYGLAPRSWEPALDDILDRLVGPVRSH
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]