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The map label for this gene is rmlA2 [H]

Identifier: 218531917

GI number: 218531917

Start: 4211749

End: 4212633

Strand: Reverse

Name: rmlA2 [H]

Synonym: Mchl_4001

Alternate gene names: 218531917

Gene position: 4212633-4211749 (Counterclockwise)

Preceding gene: 218531918

Following gene: 218531916

Centisome position: 72.91

GC content: 65.65

Gene sequence:

>885_bases
ATGAAGGGCATCGTGCTCGCCGGCGGCTCCGGCACCAGGCTCCATCCGGCCACGCTGGCGATCAATAAGCAGCTGCTGCC
GGTCTACGATAAGCCGATGATCTACTACCCGATCTCGGTGCTGATGCTCGCCGGCATTCGCGAGATCCTCGTCATCTCCT
CGCCGGAGCATCTCGGCAACTACCAGCGCCTGCTCGGCACCGGTGAGCAGTTCGGCGTCACCTTCACCTACGCCGTGCAG
CCGCGGCCCGAAGGGCTGGCCCAGGCCTTCATCATCGGCCGGGACTTCGTCGGCAGCGACGACGTGGCGCTGGTGCTCGG
CGACAACCTGTTCTTCGGCAACGGCATGAGCGACCTGCTCGCCAAGGCGCGCACCCGCAAGAGCGGGGCAACGGTGTTTG
CCTACCACGTCGATCATCCGGAGGCCTACGGCGTCGTCACCCTCGACGAGTCCGGCCGGCCCTTGCGGCTGGTGGAGAAG
CCCAAGACCCCCGAGAGCCCCTGGGCGGTGACGGGGCTGTACTTCTACGACAATCAGGTGCTCGACATCGCCGCGGAGGT
GAAGCCCTCGGATCGGGGCGAACTCGAGATCACATCCGTCAACCAGGCCTATCTCGAACGCGGCCAGCTCCATGTCGAAC
GGATGAGCCGCGGCTACGCCTGGCTCGATACGGGGACGCATGACAGCTTGCTGGAGGCGGGTGAGTTCGTGCGGGTGCTG
CAGCGCCGGCAAGGACTTCAGGTCGCCTGCCTCGAAGAGATTGCCTACCTGCAGGGCTTCATCGGCCGCGAGCAGTTGCA
GGCGCGCGGTGAGCTGTTCGCCAAAACTAGCTACGGCCAGAACCTGCTGCGCCTCGCCCGCGAGGGCCACCTTAGCATAG
CCTAA

Upstream 100 bases:

>100_bases
GCCTACGGCCTCGCGCCGCGCTCCTGGGAGCCGGCGCTCGACGACATCCTCGACCGGCTGGTCGGGCCGGTTCGCTCCCA
CTGATCAGGACGTGTTTCGT

Downstream 100 bases:

>100_bases
GGGTGCTTCACACAACGTCGGGTCACCGATCGTCTACCCCTTCGGACGCAGTGACGCGGCGGGACTTTTTGTGAGTGATG
TTTAAAGCGCCTAACAAAAA

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: G1P-TT 2; dTDP-glucose pyrophosphorylase 2; dTDP-glucose synthase 2 [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MKGIVLAGGSGTRLHPATLAINKQLLPVYDKPMIYYPISVLMLAGIREILVISSPEHLGNYQRLLGTGEQFGVTFTYAVQ
PRPEGLAQAFIIGRDFVGSDDVALVLGDNLFFGNGMSDLLAKARTRKSGATVFAYHVDHPEAYGVVTLDESGRPLRLVEK
PKTPESPWAVTGLYFYDNQVLDIAAEVKPSDRGELEITSVNQAYLERGQLHVERMSRGYAWLDTGTHDSLLEAGEFVRVL
QRRQGLQVACLEEIAYLQGFIGREQLQARGELFAKTSYGQNLLRLAREGHLSIA

Sequences:

>Translated_294_residues
MKGIVLAGGSGTRLHPATLAINKQLLPVYDKPMIYYPISVLMLAGIREILVISSPEHLGNYQRLLGTGEQFGVTFTYAVQ
PRPEGLAQAFIIGRDFVGSDDVALVLGDNLFFGNGMSDLLAKARTRKSGATVFAYHVDHPEAYGVVTLDESGRPLRLVEK
PKTPESPWAVTGLYFYDNQVLDIAAEVKPSDRGELEITSVNQAYLERGQLHVERMSRGYAWLDTGTHDSLLEAGEFVRVL
QRRQGLQVACLEEIAYLQGFIGREQLQARGELFAKTSYGQNLLRLAREGHLSIA
>Mature_294_residues
MKGIVLAGGSGTRLHPATLAINKQLLPVYDKPMIYYPISVLMLAGIREILVISSPEHLGNYQRLLGTGEQFGVTFTYAVQ
PRPEGLAQAFIIGRDFVGSDDVALVLGDNLFFGNGMSDLLAKARTRKSGATVFAYHVDHPEAYGVVTLDESGRPLRLVEK
PKTPESPWAVTGLYFYDNQVLDIAAEVKPSDRGELEITSVNQAYLERGQLHVERMSRGYAWLDTGTHDSLLEAGEFVRVL
QRRQGLQVACLEEIAYLQGFIGREQLQARGELFAKTSYGQNLLRLAREGHLSIA

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761619, Length=289, Percent_Identity=24.2214532871972, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI11761621, Length=289, Percent_Identity=24.2214532871972, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1790224, Length=287, Percent_Identity=60.9756097560976, Blast_Score=366, Evalue=1e-103,
Organism=Escherichia coli, GI1788351, Length=289, Percent_Identity=58.1314878892734, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1787488, Length=294, Percent_Identity=27.5510204081633, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788355, Length=239, Percent_Identity=26.7782426778243, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI133931050, Length=183, Percent_Identity=26.2295081967213, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320148, Length=255, Percent_Identity=29.8039215686275, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355443, Length=231, Percent_Identity=25.5411255411255, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24644084, Length=231, Percent_Identity=25.5411255411255, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 32465; Mature: 32465

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIVLAGGSGTRLHPATLAINKQLLPVYDKPMIYYPISVLMLAGIREILVISSPEHLGN
CCCEEEECCCCCEECCEEEEECCCCCCCCCCCEEEHHHHHHHHHCCCEEEEECCCHHHCH
YQRLLGTGEQFGVTFTYAVQPRPEGLAQAFIIGRDFVGSDDVALVLGDNLFFGNGMSDLL
HHHHHCCCCHHCEEEEEEECCCCCHHHEEEEEECCCCCCCCEEEEEECCEEECCCHHHHH
AKARTRKSGATVFAYHVDHPEAYGVVTLDESGRPLRLVEKPKTPESPWAVTGLYFYDNQV
HHHHCCCCCCEEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEEEEEEECCEE
LDIAAEVKPSDRGELEITSVNQAYLERGQLHVERMSRGYAWLDTGTHDSLLEAGEFVRVL
EEEEEECCCCCCCCEEEEECCHHHHHHCHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHH
QRRQGLQVACLEEIAYLQGFIGREQLQARGELFAKTSYGQNLLRLAREGHLSIA
HHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHEEEECCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKGIVLAGGSGTRLHPATLAINKQLLPVYDKPMIYYPISVLMLAGIREILVISSPEHLGN
CCCEEEECCCCCEECCEEEEECCCCCCCCCCCEEEHHHHHHHHHCCCEEEEECCCHHHCH
YQRLLGTGEQFGVTFTYAVQPRPEGLAQAFIIGRDFVGSDDVALVLGDNLFFGNGMSDLL
HHHHHCCCCHHCEEEEEEECCCCCHHHEEEEEECCCCCCCCEEEEEECCEEECCCHHHHH
AKARTRKSGATVFAYHVDHPEAYGVVTLDESGRPLRLVEKPKTPESPWAVTGLYFYDNQV
HHHHCCCCCCEEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEEEEEEECCEE
LDIAAEVKPSDRGELEITSVNQAYLERGQLHVERMSRGYAWLDTGTHDSLLEAGEFVRVL
EEEEEECCCCCCCCEEEEECCHHHHHHCHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHH
QRRQGLQVACLEEIAYLQGFIGREQLQARGELFAKTSYGQNLLRLAREGHLSIA
HHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHEEEECCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]