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The map label for this gene is fixJ [H]

Identifier: 218531743

GI number: 218531743

Start: 4025580

End: 4026188

Strand: Reverse

Name: fixJ [H]

Synonym: Mchl_3813

Alternate gene names: 218531743

Gene position: 4026188-4025580 (Counterclockwise)

Preceding gene: 218531744

Following gene: 218531742

Centisome position: 69.68

GC content: 68.31

Gene sequence:

>609_bases
ATGGCCCCTGCCCTCGTGCATGTCATCGACGACGACCCCGCCATGCGCGACTCGGTCGCGTTCCTTCTCGACACGGCCGG
CTTCGACGTGCGGCTCTACGAGGCGGGAACGGCCCTGCTGGCGCGGCTCACGCGGCCGCTCCGAGGGTGCATCCTGACCG
ATATCCGTATGCCCGACCTCGACGGGTTGGCCCTGTTCCGCTCCCTCCGCTCCGCCGGTCACACGCTTCCCATCGTGATG
ATGACGGGGCATGGCGACGTGCCGCTCGCGGTCGAGGCGATGAAGCTCGGGGCCTGTGACTTCATCGAGAAGCCGTTCGA
TGGCGAGGCGCTCCTCCGGGCGCTGCAGGCCGCCCTGGAGCAGGGTGGGCCTAACGGCTCGGTCGATCCGATCCTGCAGG
ACTTCATGCGGCGGTTGGCGACCCTGAGCGAGCGCGAGCGCCAGGTTCTCGATCATCTGGTGACCGGGGGTACCAGCAAG
GAGATCGGGCGAAGGCTCCGGATCAGCCCGCGGACCGTCGAGATCTACCGGGCCAAGGTCATGGCCAAGACGCGGGCGGG
AAGTTTGCAGGAACTGGTCCGCTGGGCCGTGCTCGCCGGCTTCGTGTGA

Upstream 100 bases:

>100_bases
CGAGGCGCATGGCGGCCGCCTCTGGGTCGAGCCCAACGCCGCGGGCGGGGCGACCTTCCGGCTGACCCTGCCGACCGCAC
CCGAGAGAGGTCGGTAACCC

Downstream 100 bases:

>100_bases
CGCCGGCTCGCGGCCGACCACGGGATGGCCGCGTTCCTGCCCTCGCGATTTCCCCGAGCCGGCCATCCGAATGCGGAGCC
CCGTCACCGATGGCGGGAGC

Product: LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MAPALVHVIDDDPAMRDSVAFLLDTAGFDVRLYEAGTALLARLTRPLRGCILTDIRMPDLDGLALFRSLRSAGHTLPIVM
MTGHGDVPLAVEAMKLGACDFIEKPFDGEALLRALQAALEQGGPNGSVDPILQDFMRRLATLSERERQVLDHLVTGGTSK
EIGRRLRISPRTVEIYRAKVMAKTRAGSLQELVRWAVLAGFV

Sequences:

>Translated_202_residues
MAPALVHVIDDDPAMRDSVAFLLDTAGFDVRLYEAGTALLARLTRPLRGCILTDIRMPDLDGLALFRSLRSAGHTLPIVM
MTGHGDVPLAVEAMKLGACDFIEKPFDGEALLRALQAALEQGGPNGSVDPILQDFMRRLATLSERERQVLDHLVTGGTSK
EIGRRLRISPRTVEIYRAKVMAKTRAGSLQELVRWAVLAGFV
>Mature_201_residues
APALVHVIDDDPAMRDSVAFLLDTAGFDVRLYEAGTALLARLTRPLRGCILTDIRMPDLDGLALFRSLRSAGHTLPIVMM
TGHGDVPLAVEAMKLGACDFIEKPFDGEALLRALQAALEQGGPNGSVDPILQDFMRRLATLSERERQVLDHLVTGGTSKE
IGRRLRISPRTVEIYRAKVMAKTRAGSLQELVRWAVLAGFV

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=201, Percent_Identity=34.8258706467662, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788905, Length=122, Percent_Identity=34.4262295081967, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1790437, Length=173, Percent_Identity=30.0578034682081, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788550, Length=113, Percent_Identity=31.858407079646, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786747, Length=213, Percent_Identity=25.8215962441315, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1790102, Length=192, Percent_Identity=32.2916666666667, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788222, Length=167, Percent_Identity=28.1437125748503, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789809, Length=112, Percent_Identity=33.9285714285714, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22074; Mature: 21943

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPALVHVIDDDPAMRDSVAFLLDTAGFDVRLYEAGTALLARLTRPLRGCILTDIRMPDL
CCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCC
DGLALFRSLRSAGHTLPIVMMTGHGDVPLAVEAMKLGACDFIEKPFDGEALLRALQAALE
HHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHH
QGGPNGSVDPILQDFMRRLATLSERERQVLDHLVTGGTSKEIGRRLRISPRTVEIYRAKV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHH
MAKTRAGSLQELVRWAVLAGFV
HHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
APALVHVIDDDPAMRDSVAFLLDTAGFDVRLYEAGTALLARLTRPLRGCILTDIRMPDL
CCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCC
DGLALFRSLRSAGHTLPIVMMTGHGDVPLAVEAMKLGACDFIEKPFDGEALLRALQAALE
HHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHH
QGGPNGSVDPILQDFMRRLATLSERERQVLDHLVTGGTSKEIGRRLRISPRTVEIYRAKV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHH
MAKTRAGSLQELVRWAVLAGFV
HHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]