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The map label for this gene is fixK [H]

Identifier: 218531742

GI number: 218531742

Start: 4024354

End: 4025043

Strand: Reverse

Name: fixK [H]

Synonym: Mchl_3812

Alternate gene names: 218531742

Gene position: 4025043-4024354 (Counterclockwise)

Preceding gene: 218531743

Following gene: 218531740

Centisome position: 69.66

GC content: 69.86

Gene sequence:

>690_bases
ATGGGCACGAAGCCGACGCCGAGATCCATTCCGGTCGCTCCGGGCTCGCGCACGCCGCGCCGGAGCATCCACGAATGCGG
CCTCTTCGCCGATTGCCCCGATCTCGTCGGCACACCGTTCTCCTACGCCCGCGAGGAGGAGGTCTACGGCGAGGGCGAGG
ACGCCGAGTTCGTCTACCGGGTCGTCGAGGGGGCGGTGCGCTCCCACAAGATTCTGAGCGACGGCCGGCGCCAGATCACC
GGCTTCCACCTGGCGGGCAACCTGTTCGGGTTCGAGCAGGGCGACACCCACTGGCACACGGCAGAGGCGCTCTCGGACAC
CCGGGTGCTGATCTTCCAGCGGCGGCAGATCGAGCGGGCCGCCACCCGCAGCGCCGAAGTCGCCTGCCAGCTCTGGGGCA
TGGCGACGACCAGCCTGCGCGACGCCCAGGATCTCATGCTCCTGCTCGGCCGCCGTTCCGCCCAGGAGCGCGTCGCCGCC
TTTCTCATCGAGGTCGATGGCCGCTTCGGCGGCACCGGGGCGTTCGCCCTGCCGATGACCCGACGCGATATCGCCGACTA
TCTCGGCCTCACCATCGAGACCGTCTCACGCACCTTCTCGCAGCTCGAGGAGAACGGGGCGCTGCGGCGCTCTGGAAGCC
GGCAGATCACGCTTCACCGCGGCCGCCTCCGCCACGTGGTCGAGAACTGA

Upstream 100 bases:

>100_bases
AGTTTGCCTTAAGCAGGACTACTTAAGAGAGTATCCGGAATTTCGTCGGTGCCGTGGCGCGACCACTCTCGTCTCATCGA
GACGGATGAGGAGCGAACCG

Downstream 100 bases:

>100_bases
GACGGCCTCACGCCCGGCTTTCGTGTCCGGCCGCCGCGGACAGGGATGCGCCGTCCGCCACCGCTGCGACGACGCGCTCG
CGCATCGTCGCCACGGCCTC

Product: Crp/Fnr family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MGTKPTPRSIPVAPGSRTPRRSIHECGLFADCPDLVGTPFSYAREEEVYGEGEDAEFVYRVVEGAVRSHKILSDGRRQIT
GFHLAGNLFGFEQGDTHWHTAEALSDTRVLIFQRRQIERAATRSAEVACQLWGMATTSLRDAQDLMLLLGRRSAQERVAA
FLIEVDGRFGGTGAFALPMTRRDIADYLGLTIETVSRTFSQLEENGALRRSGSRQITLHRGRLRHVVEN

Sequences:

>Translated_229_residues
MGTKPTPRSIPVAPGSRTPRRSIHECGLFADCPDLVGTPFSYAREEEVYGEGEDAEFVYRVVEGAVRSHKILSDGRRQIT
GFHLAGNLFGFEQGDTHWHTAEALSDTRVLIFQRRQIERAATRSAEVACQLWGMATTSLRDAQDLMLLLGRRSAQERVAA
FLIEVDGRFGGTGAFALPMTRRDIADYLGLTIETVSRTFSQLEENGALRRSGSRQITLHRGRLRHVVEN
>Mature_228_residues
GTKPTPRSIPVAPGSRTPRRSIHECGLFADCPDLVGTPFSYAREEEVYGEGEDAEFVYRVVEGAVRSHKILSDGRRQITG
FHLAGNLFGFEQGDTHWHTAEALSDTRVLIFQRRQIERAATRSAEVACQLWGMATTSLRDAQDLMLLLGRRSAQERVAAF
LIEVDGRFGGTGAFALPMTRRDIADYLGLTIETVSRTFSQLEENGALRRSGSRQITLHRGRLRHVVEN

Specific function: FixK is a protein that regulates nitrogen fixation genes both positively and negatively. FixK appears to repress its own expression and that of nifA. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=173, Percent_Identity=32.9479768786127, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25530; Mature: 25399

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS50042 CNMP_BINDING_3 ; PS00042 HTH_CRP_1 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTKPTPRSIPVAPGSRTPRRSIHECGLFADCPDLVGTPFSYAREEEVYGEGEDAEFVYR
CCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHCCCHHHHHHHHHCCCCCCHHHHHH
VVEGAVRSHKILSDGRRQITGFHLAGNLFGFEQGDTHWHTAEALSDTRVLIFQRRQIERA
HHHHHHHHHHHHHCCHHHHEEEEEECCCCCCCCCCCCCHHHHHHCCCHHEEHHHHHHHHH
ATRSAEVACQLWGMATTSLRDAQDLMLLLGRRSAQERVAAFLIEVDGRFGGTGAFALPMT
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCEEECCCH
RRDIADYLGLTIETVSRTFSQLEENGALRRSGSRQITLHRGRLRHVVEN
HHHHHHHHCHHHHHHHHHHHHHHHCCCHHCCCCCEEEEEHHHHHHHHCC
>Mature Secondary Structure 
GTKPTPRSIPVAPGSRTPRRSIHECGLFADCPDLVGTPFSYAREEEVYGEGEDAEFVYR
CCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHCCCHHHHHHHHHCCCCCCHHHHHH
VVEGAVRSHKILSDGRRQITGFHLAGNLFGFEQGDTHWHTAEALSDTRVLIFQRRQIERA
HHHHHHHHHHHHHCCHHHHEEEEEECCCCCCCCCCCCCHHHHHHCCCHHEEHHHHHHHHH
ATRSAEVACQLWGMATTSLRDAQDLMLLLGRRSAQERVAAFLIEVDGRFGGTGAFALPMT
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCEEECCCH
RRDIADYLGLTIETVSRTFSQLEENGALRRSGSRQITLHRGRLRHVVEN
HHHHHHHHCHHHHHHHHHHHHHHHCCCHHCCCCCEEEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2663474; 11481432 [H]