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The map label for this gene is gyrB [H]

Identifier: 218531372

GI number: 218531372

Start: 3626604

End: 3628661

Strand: Reverse

Name: gyrB [H]

Synonym: Mchl_3440

Alternate gene names: 218531372

Gene position: 3628661-3626604 (Counterclockwise)

Preceding gene: 218531374

Following gene: 218531367

Centisome position: 62.8

GC content: 68.03

Gene sequence:

>2058_bases
GTGAGCGATCCGGCACGCGACCTGTTCGGGCCTGGTGCCAAGCCTCCCCCCGCGGAGGCCGAGCGCCGCCGCCGCCTCAC
CCCCGTGGCACCGTCTCCGGCGCCCGCGCCGGAAACGGCGGAGGCGGGTTACGACGCCTCGGCGATCGAGGTACTGGAGG
GGCTGGAGCCGGTGCGGCGCCGGCCGGGCATGTATATCGGCGGCACCGACGAGCGGGCGTTGCACCACCTCTTCGCCGAG
GTGATCGACAACTCAATGGACGAAGCGGTCGCGGGCCACGCGAGCTTCATCGAGGTGGAGCTGGAAGAATCGGGCTCGCT
CGTCGTCACCGATAACGGCCGCGGCATCCCCGTCGATCCGCATCCGAAATTCCCGGGCAAATCCGCACTCGAGGTCATCA
TGACCACGCTGCACGCGGGCGGTAAGTTCGACTCGAAGGTCTACGAGACCTCCGGCGGTCTGCACGGCGTCGGCATCTCC
GTCGTCAACGCGCTCTCGGACGTGCTCGAGGTCGAGGTCGCGCGCAATCAGACCCTCTACCGGCAGTGCTTTTCCCGCGG
TCATGCGCAGGGAAGCCTGGAGACGATCGGGCGGGTGCAGAACCGGCGCGGCACGCGGGTTCGCTTCCATCCCGACGCGC
AGATCTTCGGCTCGCTCAAGTTCGACCCGCGCCGCCTGTTCAAGATGGCCCGCTCCAAGGCCTATCTGTTCGGCGGCGTC
GAGATCCGCTGGCGCTGCGCCCCGGCCCTGCTCGAAGGGCTGGAGGATGTGCCGGCGGAGGCCGTGCACCGCTTCCCCGG
CGGGCTCTCGGACTATCTTGCCCGCGACATCGAGGGGAAGGAGCTCGTCCTCGATTCGATCTTCTCGGGGAAGATCACCA
AGCCCGGCTCGCACGGCTCGCTCGAATGGGCGGTCGCCTGGACGGTCGCCGATGACGGCGTCTCGCACTCGTACTGCAAC
ACGATCCCGACGCCGGAGGGCGGCACCCACGAGGCGGGCCTGCGCGTCGCGCTGCTGCGGGCGCTCCGCGAGCATGCCGA
GCGCGTGAACCAAGCCAAGCGCATGACGGCGGTGACCACCGACGACGTGATGGCGACCTGCGCCTCGATGCTTTCGGTCT
TCATCCGCGAGCCGGAATTCCAGGGGCAGACCAAGGACAAGCTCGCGACGGTGGAGGCCTCCCGCATCGTCGAGACGGCG
ATTCGCGATGCCTTCGACCACTGGCTCGCGGCCTCGCCCGCCCAGGCCAACAAGCTGCTCGACTGGGTGATCGACCGGGC
GGAGGAGCGCCTGCGCCGCCGCCAGGAAAAGGAGGTCGCCCGCAAGAGCGCCACCCGCAAGCTGCGCCTGCCCGGCAAGC
TCGCCGATTGCTCGGCCGCCGGGACGGTGGGCTCCGAGATCTTCATCGTCGAGGGCGACTCGGCCGGTGGCTCCGCCAAG
CAGGCCCGCGACCGCGCCACGCAAGCCGTGCTGCCGTTGCGAGGAAAAATCCTGAACGTGGCCTCGGCGAGCCGCGACAA
GCTCGGCGCCAACCAGCTCATCTCGGATCTGACGCTGGCGCTCGGCTGCGGCACCGGCGCGAACTTCCGCGAGGCGGACC
TTCGCTACGACCGGGTCATCATCATGACCGACGCGGATGTGGACGGCGCCCACATCGCCTCGCTCCTCATCACCTTCTTC
TACCGGCAGATGCCTAAGCTCATCGACAAGGGGCATCTCTACCTCGCGATCCCGCCGCTCTACCGGATCAGCCAGGGCGC
GAAATCGGCCTATGCCCGCGACGATGCGGACAAGGAGCGGATCATCAAGACCGTGTTCAAGAACGGCAAGGTCGAGATCG
GCCGCTTCAAGGGCCTCGGCGAGATGATGCCGGCGCAGTTGAAGGAGACGACGATGGACCCGAAGAAGCGCACGCTGCTG
CGCGTCGCGATCCTCGACGAGGCGCGGGAATCGACCGGCGATACGGTGGAGCGCTTGATGGGCAACAAGCCCGAAGCGCG
CTTCGCCTTCATTACCGAGCGGGCGTCGTTCGCGGATGGGACGGAGTTGGATATCTGA

Upstream 100 bases:

>100_bases
GGAAGCGGATAGGAGACTTTTTGTTTCCTGTTTGTTCCGCTACAGTGAGGGTATGAAGAGCCATGCAGCCCGTCTCCGAC
CCGATTCGAGGAGCCGTCGC

Downstream 100 bases:

>100_bases
TCCGCTCCGGCATCGCGACAGGCAGAACGAGCTGTCGCGATGGATGCGCCCGGAAACACCCTCCCCGTCAGCCCGGCCCG
TTCGAGCAGGTCAGGAGGGT

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 685; Mature: 684

Protein sequence:

>685_residues
MSDPARDLFGPGAKPPPAEAERRRRLTPVAPSPAPAPETAEAGYDASAIEVLEGLEPVRRRPGMYIGGTDERALHHLFAE
VIDNSMDEAVAGHASFIEVELEESGSLVVTDNGRGIPVDPHPKFPGKSALEVIMTTLHAGGKFDSKVYETSGGLHGVGIS
VVNALSDVLEVEVARNQTLYRQCFSRGHAQGSLETIGRVQNRRGTRVRFHPDAQIFGSLKFDPRRLFKMARSKAYLFGGV
EIRWRCAPALLEGLEDVPAEAVHRFPGGLSDYLARDIEGKELVLDSIFSGKITKPGSHGSLEWAVAWTVADDGVSHSYCN
TIPTPEGGTHEAGLRVALLRALREHAERVNQAKRMTAVTTDDVMATCASMLSVFIREPEFQGQTKDKLATVEASRIVETA
IRDAFDHWLAASPAQANKLLDWVIDRAEERLRRRQEKEVARKSATRKLRLPGKLADCSAAGTVGSEIFIVEGDSAGGSAK
QARDRATQAVLPLRGKILNVASASRDKLGANQLISDLTLALGCGTGANFREADLRYDRVIIMTDADVDGAHIASLLITFF
YRQMPKLIDKGHLYLAIPPLYRISQGAKSAYARDDADKERIIKTVFKNGKVEIGRFKGLGEMMPAQLKETTMDPKKRTLL
RVAILDEARESTGDTVERLMGNKPEARFAFITERASFADGTELDI

Sequences:

>Translated_685_residues
MSDPARDLFGPGAKPPPAEAERRRRLTPVAPSPAPAPETAEAGYDASAIEVLEGLEPVRRRPGMYIGGTDERALHHLFAE
VIDNSMDEAVAGHASFIEVELEESGSLVVTDNGRGIPVDPHPKFPGKSALEVIMTTLHAGGKFDSKVYETSGGLHGVGIS
VVNALSDVLEVEVARNQTLYRQCFSRGHAQGSLETIGRVQNRRGTRVRFHPDAQIFGSLKFDPRRLFKMARSKAYLFGGV
EIRWRCAPALLEGLEDVPAEAVHRFPGGLSDYLARDIEGKELVLDSIFSGKITKPGSHGSLEWAVAWTVADDGVSHSYCN
TIPTPEGGTHEAGLRVALLRALREHAERVNQAKRMTAVTTDDVMATCASMLSVFIREPEFQGQTKDKLATVEASRIVETA
IRDAFDHWLAASPAQANKLLDWVIDRAEERLRRRQEKEVARKSATRKLRLPGKLADCSAAGTVGSEIFIVEGDSAGGSAK
QARDRATQAVLPLRGKILNVASASRDKLGANQLISDLTLALGCGTGANFREADLRYDRVIIMTDADVDGAHIASLLITFF
YRQMPKLIDKGHLYLAIPPLYRISQGAKSAYARDDADKERIIKTVFKNGKVEIGRFKGLGEMMPAQLKETTMDPKKRTLL
RVAILDEARESTGDTVERLMGNKPEARFAFITERASFADGTELDI
>Mature_684_residues
SDPARDLFGPGAKPPPAEAERRRRLTPVAPSPAPAPETAEAGYDASAIEVLEGLEPVRRRPGMYIGGTDERALHHLFAEV
IDNSMDEAVAGHASFIEVELEESGSLVVTDNGRGIPVDPHPKFPGKSALEVIMTTLHAGGKFDSKVYETSGGLHGVGISV
VNALSDVLEVEVARNQTLYRQCFSRGHAQGSLETIGRVQNRRGTRVRFHPDAQIFGSLKFDPRRLFKMARSKAYLFGGVE
IRWRCAPALLEGLEDVPAEAVHRFPGGLSDYLARDIEGKELVLDSIFSGKITKPGSHGSLEWAVAWTVADDGVSHSYCNT
IPTPEGGTHEAGLRVALLRALREHAERVNQAKRMTAVTTDDVMATCASMLSVFIREPEFQGQTKDKLATVEASRIVETAI
RDAFDHWLAASPAQANKLLDWVIDRAEERLRRRQEKEVARKSATRKLRLPGKLADCSAAGTVGSEIFIVEGDSAGGSAKQ
ARDRATQAVLPLRGKILNVASASRDKLGANQLISDLTLALGCGTGANFREADLRYDRVIIMTDADVDGAHIASLLITFFY
RQMPKLIDKGHLYLAIPPLYRISQGAKSAYARDDADKERIIKTVFKNGKVEIGRFKGLGEMMPAQLKETTMDPKKRTLLR
VAILDEARESTGDTVERLMGNKPEARFAFITERASFADGTELDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=635, Percent_Identity=25.8267716535433, Blast_Score=150, Evalue=5e-36,
Organism=Homo sapiens, GI19913406, Length=641, Percent_Identity=25.1170046801872, Blast_Score=144, Evalue=3e-34,
Organism=Escherichia coli, GI1789408, Length=640, Percent_Identity=43.4375, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI48994957, Length=560, Percent_Identity=43.5714285714286, Blast_Score=421, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17535065, Length=592, Percent_Identity=23.4797297297297, Blast_Score=128, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI212645845, Length=579, Percent_Identity=24.8704663212435, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI212645657, Length=206, Percent_Identity=27.6699029126214, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=637, Percent_Identity=24.3328100470958, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17136538, Length=583, Percent_Identity=25.2144082332762, Blast_Score=127, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 74838; Mature: 74707

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPARDLFGPGAKPPPAEAERRRRLTPVAPSPAPAPETAEAGYDASAIEVLEGLEPVRR
CCCCCCHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHH
RPGMYIGGTDERALHHLFAEVIDNSMDEAVAGHASFIEVELEESGSLVVTDNGRGIPVDP
CCCEEECCCHHHHHHHHHHHHHHCHHHHHHCCCCEEEEEEEECCCCEEEECCCCCCCCCC
HPKFPGKSALEVIMTTLHAGGKFDSKVYETSGGLHGVGISVVNALSDVLEVEVARNQTLY
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHH
RQCFSRGHAQGSLETIGRVQNRRGTRVRFHPDAQIFGSLKFDPRRLFKMARSKAYLFGGV
HHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCHHHEECCCCCHHHHHHHHHCCEEEEECE
EIRWRCAPALLEGLEDVPAEAVHRFPGGLSDYLARDIEGKELVLDSIFSGKITKPGSHGS
EEEEEECHHHHHHHHHCCHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCC
LEWAVAWTVADDGVSHSYCNTIPTPEGGTHEAGLRVALLRALREHAERVNQAKRMTAVTT
EEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
DDVMATCASMLSVFIREPEFQGQTKDKLATVEASRIVETAIRDAFDHWLAASPAQANKLL
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
DWVIDRAEERLRRRQEKEVARKSATRKLRLPGKLADCSAAGTVGSEIFIVEGDSAGGSAK
HHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCHH
QARDRATQAVLPLRGKILNVASASRDKLGANQLISDLTLALGCGTGANFREADLRYDRVI
HHHHHHHHHHHHHCCHHEEHHHCCHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
IMTDADVDGAHIASLLITFFYRQMPKLIDKGHLYLAIPPLYRISQGAKSAYARDDADKER
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHCHHHHHCCCCCHHHH
IIKTVFKNGKVEIGRFKGLGEMMPAQLKETTMDPKKRTLLRVAILDEARESTGDTVERLM
HHHHHHHCCCEEEEHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
GNKPEARFAFITERASFADGTELDI
CCCCCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SDPARDLFGPGAKPPPAEAERRRRLTPVAPSPAPAPETAEAGYDASAIEVLEGLEPVRR
CCCCCHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHH
RPGMYIGGTDERALHHLFAEVIDNSMDEAVAGHASFIEVELEESGSLVVTDNGRGIPVDP
CCCEEECCCHHHHHHHHHHHHHHCHHHHHHCCCCEEEEEEEECCCCEEEECCCCCCCCCC
HPKFPGKSALEVIMTTLHAGGKFDSKVYETSGGLHGVGISVVNALSDVLEVEVARNQTLY
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHH
RQCFSRGHAQGSLETIGRVQNRRGTRVRFHPDAQIFGSLKFDPRRLFKMARSKAYLFGGV
HHHHHHCCCCCCHHHHHHHHCCCCCEEEECCCHHHEECCCCCHHHHHHHHHCCEEEEECE
EIRWRCAPALLEGLEDVPAEAVHRFPGGLSDYLARDIEGKELVLDSIFSGKITKPGSHGS
EEEEEECHHHHHHHHHCCHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCC
LEWAVAWTVADDGVSHSYCNTIPTPEGGTHEAGLRVALLRALREHAERVNQAKRMTAVTT
EEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
DDVMATCASMLSVFIREPEFQGQTKDKLATVEASRIVETAIRDAFDHWLAASPAQANKLL
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
DWVIDRAEERLRRRQEKEVARKSATRKLRLPGKLADCSAAGTVGSEIFIVEGDSAGGSAK
HHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCHH
QARDRATQAVLPLRGKILNVASASRDKLGANQLISDLTLALGCGTGANFREADLRYDRVI
HHHHHHHHHHHHHCCHHEEHHHCCHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEE
IMTDADVDGAHIASLLITFFYRQMPKLIDKGHLYLAIPPLYRISQGAKSAYARDDADKER
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHCHHHHHCCCCCHHHH
IIKTVFKNGKVEIGRFKGLGEMMPAQLKETTMDPKKRTLLRVAILDEARESTGDTVERLM
HHHHHHHCCCEEEEHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
GNKPEARFAFITERASFADGTELDI
CCCCCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9797224 [H]