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The map label for this gene is lpxK

Identifier: 218531145

GI number: 218531145

Start: 3374790

End: 3375773

Strand: Reverse

Name: lpxK

Synonym: Mchl_3195

Alternate gene names: 218531145

Gene position: 3375773-3374790 (Counterclockwise)

Preceding gene: 218531146

Following gene: 218531143

Centisome position: 58.43

GC content: 74.39

Gene sequence:

>984_bases
ATGCGTCCGCCCGGCTTCTGGACCCGGCCGCCCACGCATCCGCTCGCGCGGCTGCTGGCCCCCGTCGGCCGGGTCTATGG
CGGCCTGACCGCCGACCGGATGGACCGGCCCGGCGCGGAACCGCCCTACCCGGTCCTGTGCGTCGGCAACTTCACCCTCG
GCGGCGCCGGCAAGACGCCGACGGCCCTCGCCCTCGTCCGCCTGCTGCGCGATCTCGGCCGAACGCCCGCCCTGCTGAGC
CGGGGCTATGGCGGCCGGCTCGCCGGGCCGCTGGTCGTCGATCCCGCGCGGCACGCGGCGGCGGAGGTCGGCGACGAGCC
GCTGCTTCTGGCGCAGGCGGCGCCCACCATCGTCGCTCGTGACCGCCCGTCCGGCGCCCGCCTCTGCGCCGCGTCCGGTG
CCGACGTGATCGTTATGGATGACGGCCTGCAGAATCCGAGCCTGACCAAGTCGCTGTCACTCGCGGTGGTCGATGGGGGT
GTTGGCCTCGGCAACGGCCTGCCCTTCCCCGCCGGGCCGCTGCGCGCGCCGCTGGCCCGGCAATGGCCTCATGTCGCCGG
CCTCGTGCTGGTCGGCGAGGGCAGCCCCGGCGAGGCGATGGCGGCGGAGGCGGAGAGCCGTGGCCTACCGGTCCACCGCG
CCCGTCTCGTTCCCGAAGCCGGGTCGGACTGGGCCGGTCGCCGCGTCGTCGCCTTTGCCGGGATCGGTCGTCCGCAGAAG
TTCTTCGAGACGCTGCGGAGCCTGGGCGCCGAGATCGTGGCGGAATGGGCCTTTCCCGACCATCACCCCTATCGGCCGGG
GGATTGGACGGCTTTGTCGGCGCTCGCCGCGCGCGAGGGCGCAAGCCTCGTCACGACGGAGAAGGATGCGGTGCGTTTGC
CGGCCGAGGCGCGCACGGCGGTGGCGGTGCTGCGGGTCGCCCTTGCTTTTGCCGACGAAACCCGTCTTCGCCAGCAGCTC
GCGGCAGCCTTCCCACGCGCCTGA

Upstream 100 bases:

>100_bases
CTCCTGCCCTTCGAGGGCGCGGTTGGGCGCACCTTCGCGGTGCTCGAACCCTACGTCGCGCAGATGAAGCTCTCCGCGCG
CGAGCGCGACTGAGGCCGCG

Downstream 100 bases:

>100_bases
CGCGGGCGCCGTTCACCGGCAGGAACCGAAGGCGGTCCGACCCAGTCGGTCGAGGTCGGGCGGCGGACGGCCCGTCCTGC
CGTCAACGCGCCGCGCCGGT

Product: tetraacyldisaccharide 4'-kinase

Products: NA

Alternate protein names: Lipid A 4'-kinase

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MRPPGFWTRPPTHPLARLLAPVGRVYGGLTADRMDRPGAEPPYPVLCVGNFTLGGAGKTPTALALVRLLRDLGRTPALLS
RGYGGRLAGPLVVDPARHAAAEVGDEPLLLAQAAPTIVARDRPSGARLCAASGADVIVMDDGLQNPSLTKSLSLAVVDGG
VGLGNGLPFPAGPLRAPLARQWPHVAGLVLVGEGSPGEAMAAEAESRGLPVHRARLVPEAGSDWAGRRVVAFAGIGRPQK
FFETLRSLGAEIVAEWAFPDHHPYRPGDWTALSALAAREGASLVTTEKDAVRLPAEARTAVAVLRVALAFADETRLRQQL
AAAFPRA

Sequences:

>Translated_327_residues
MRPPGFWTRPPTHPLARLLAPVGRVYGGLTADRMDRPGAEPPYPVLCVGNFTLGGAGKTPTALALVRLLRDLGRTPALLS
RGYGGRLAGPLVVDPARHAAAEVGDEPLLLAQAAPTIVARDRPSGARLCAASGADVIVMDDGLQNPSLTKSLSLAVVDGG
VGLGNGLPFPAGPLRAPLARQWPHVAGLVLVGEGSPGEAMAAEAESRGLPVHRARLVPEAGSDWAGRRVVAFAGIGRPQK
FFETLRSLGAEIVAEWAFPDHHPYRPGDWTALSALAAREGASLVTTEKDAVRLPAEARTAVAVLRVALAFADETRLRQQL
AAAFPRA
>Mature_327_residues
MRPPGFWTRPPTHPLARLLAPVGRVYGGLTADRMDRPGAEPPYPVLCVGNFTLGGAGKTPTALALVRLLRDLGRTPALLS
RGYGGRLAGPLVVDPARHAAAEVGDEPLLLAQAAPTIVARDRPSGARLCAASGADVIVMDDGLQNPSLTKSLSLAVVDGG
VGLGNGLPFPAGPLRAPLARQWPHVAGLVLVGEGSPGEAMAAEAESRGLPVHRARLVPEAGSDWAGRRVVAFAGIGRPQK
FFETLRSLGAEIVAEWAFPDHHPYRPGDWTALSALAAREGASLVTTEKDAVRLPAEARTAVAVLRVALAFADETRLRQQL
AAAFPRA

Specific function: Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1- P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA)

COG id: COG1663

COG function: function code M; Tetraacyldisaccharide-1-P 4'-kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lpxK family

Homologues:

Organism=Escherichia coli, GI1787144, Length=296, Percent_Identity=36.1486486486487, Blast_Score=125, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPXK_METC4 (B7KT03)

Other databases:

- EMBL:   CP001298
- RefSeq:   YP_002421961.1
- GeneID:   7114194
- GenomeReviews:   CP001298_GR
- KEGG:   mch:Mchl_3195
- HOGENOM:   HBG663914
- OMA:   VILMDDG
- ProtClustDB:   CLSK2331442
- HAMAP:   MF_00409
- InterPro:   IPR003758
- TIGRFAMs:   TIGR00682

Pfam domain/function: PF02606 LpxK

EC number: =2.7.1.130

Molecular weight: Translated: 34167; Mature: 34167

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPPGFWTRPPTHPLARLLAPVGRVYGGLTADRMDRPGAEPPYPVLCVGNFTLGGAGKTP
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCEEEECCEEECCCCCCH
TALALVRLLRDLGRTPALLSRGYGGRLAGPLVVDPARHAAAEVGDEPLLLAQAAPTIVAR
HHHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEECCHHHHHHHCCCCCEEEEECCCEEEEC
DRPSGARLCAASGADVIVMDDGLQNPSLTKSLSLAVVDGGVGLGNGLPFPAGPLRAPLAR
CCCCCCEEEECCCCCEEEECCCCCCCCCHHEEEEEEEECCCCCCCCCCCCCCCCCCCHHH
QWPHVAGLVLVGEGSPGEAMAAEAESRGLPVHRARLVPEAGSDWAGRRVVAFAGIGRPQK
CCCCEEEEEEEECCCCCCHHHHHHHHCCCCHHHHEECCCCCCCCCCCEEEEEECCCCHHH
FFETLRSLGAEIVAEWAFPDHHPYRPGDWTALSALAAREGASLVTTEKDAVRLPAEARTA
HHHHHHHHHHHHEEHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCEECCHHHHHH
VAVLRVALAFADETRLRQQLAAAFPRA
HHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRPPGFWTRPPTHPLARLLAPVGRVYGGLTADRMDRPGAEPPYPVLCVGNFTLGGAGKTP
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCEEEECCEEECCCCCCH
TALALVRLLRDLGRTPALLSRGYGGRLAGPLVVDPARHAAAEVGDEPLLLAQAAPTIVAR
HHHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEECCHHHHHHHCCCCCEEEEECCCEEEEC
DRPSGARLCAASGADVIVMDDGLQNPSLTKSLSLAVVDGGVGLGNGLPFPAGPLRAPLAR
CCCCCCEEEECCCCCEEEECCCCCCCCCHHEEEEEEEECCCCCCCCCCCCCCCCCCCHHH
QWPHVAGLVLVGEGSPGEAMAAEAESRGLPVHRARLVPEAGSDWAGRRVVAFAGIGRPQK
CCCCEEEEEEEECCCCCCHHHHHHHHCCCCHHHHEECCCCCCCCCCCEEEEEECCCCHHH
FFETLRSLGAEIVAEWAFPDHHPYRPGDWTALSALAAREGASLVTTEKDAVRLPAEARTA
HHHHHHHHHHHHEEHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCEECCHHHHHH
VAVLRVALAFADETRLRQQLAAAFPRA
HHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA